Abstract:
OBJECTIVE:To develop a reliable, reproducible, and sensitive method for investigating gene-expression profiles from individual human oocytes. DESIGN:Five commercially available protocols were investigated for their efficiency to amplify messenger RNA (mRNA) from 54 single human oocytes. Protocols resulting in sufficient yields were further validated using microarray technology. For the validation, mRNA was isolated from 25 human oocytes. To eliminate biological variation, RNA from 13 human oocytes was pooled together and split into 12 identical samples for further mRNA amplification. From 12 oocytes, mRNA was individually isolated. SETTING:University medical center and university microarray laboratory. PATIENT(S):Couples undergoing intracytoplasmic sperm injection treatment were asked to donate their immature oocytes for research, and written informed consent was obtained in all cases. Seventy-nine human oocytes were used in total. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Amplification efficiency and microarray profiles. RESULT(S):Two of the five protocols (WT-Ovation One-Direct and Arcturus RiboAMp HS Plus) resulted in sufficient yields and high success rates and were further validated for their performance in obtaining reliable, reproducible, and sensitive expression profiles from individual human oocytes. Evaluation of these two protocols demonstrated that they both displayed low technical variation and produced highly reproducible profiles (r ≥ 0.95). One of them identified significantly more transcripts but also had a higher number of false discoveries. CONCLUSION(S):Two protocols generated ample amounts of mRNA for (quantitative) polymerase chain reaction, microarray, and sequencing techniques. Further validation using a design that discriminates between biological and technical variation showed that both protocols can be used for gene-expression profiling of individual human oocytes.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Mantikou E,Bruning O,Mastenbroek S,Repping S,Breit TM,de Jong Mdoi
10.1016/j.fertnstert.2015.10.025subject
Has Abstractpub_date
2016-02-01 00:00:00pages
511-9.e4issue
2eissn
0015-0282issn
1556-5653pii
S0015-0282(15)02036-1journal_volume
105pub_type
杂志文章abstract::The present study was undertaken to investigate not only the effectiveness of bromoergocryptine therapy in 13 women with amenorrhea-galactorrhea and hyperprolactinemia without evidence of organic pathology, but also to assess the value of pretreatment evaluation in predicting the response to therapy. Sella turcica tom...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)42490-8
更新日期:1977-04-01 00:00:00
abstract::Human menopausal gonadotropins (hMG) and clomiphene citrate (CC), either alone or in combination, are frequently used for in vitro fertilization (IVF) in an attempt to maximize the number of oocytes recovered and the number of embryos transferred. However, direct comparison of the relative efficacy of these protocols ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)49889-4
更新日期:1986-12-01 00:00:00
abstract::Effects of single subcutaneous doses (1, 5, 20, and 100 micrograms) of nafarelin, a potent gonadotropin-releasing hormone agonist, on the physiologic events of the human menstrual cycle were studied in 28 normal women. Nafarelin entered the circulation rapidly after injection. Peak concentrations were observed within ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48432-3
更新日期:1985-03-01 00:00:00
abstract::This document will outline new delivery systems and contraceptive formulations, summarize recent advances in emergency contraception, and review the effects of hormonal contraception on cancer risks, cardiovascular disease, and bone. ...
journal_title:Fertility and sterility
pub_type: 指南,杂志文章,实务指引
doi:10.1016/j.fertnstert.2004.07.935
更新日期:2004-09-01 00:00:00
abstract:OBJECTIVE:To evaluate whether the efficacy of naltrexone administration in patients with hypothalamic amenorrhea correlates to the response to an acute naloxone test. DESIGN:Thirty patients with hypothalamic amenorrhea associated with weight loss were studied. After naloxone test (4 mg in bolus IV) patients were divid...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(16)57908-4
更新日期:1995-11-01 00:00:00
abstract::Websites for Society for Assisted Reproductive Technology-affiliated clinics fail to meet most American Medical Association health information guidelines. Professional organizations in reproductive medicine need to standardize the accuracy and appropriateness of online reproductive health information. ...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2004.11.024
更新日期:2005-03-01 00:00:00
abstract::The morphologic characteristics of 85 cryptorchid and 63 contralateral (scrotal) testes showing histologic changes of puberty were studied. Spermatogenesis was not only delayed on the cryptorchid side but also hypocellular in all cases. The scrotal testes appeared hypocellular in 82.5% of the cases. Repetitive biopsie...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)46346-6
更新日期:1982-06-01 00:00:00
abstract::Oocytes are complex cells comprising many cellular systems, each of which is essential for proper oocyte function. Methods to assess the diverse and critical cellular systems in oocytes derived from in vitro maturation are badly needed. ...
journal_title:Fertility and sterility
pub_type: 评论,杂志文章,评审
doi:10.1016/j.fertnstert.2005.12.009
更新日期:2006-04-01 00:00:00
abstract:OBJECTIVE:To investigate whether treatment with progestogens in the first trimester of pregnancy would decrease the incidence of miscarriage in women with a history of unexplained recurrent miscarriage. DESIGN:Systematic review and meta-analysis. SETTING:Not applicable. PATIENT(S):Women with a history of unexplained...
journal_title:Fertility and sterility
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.fertnstert.2016.10.031
更新日期:2017-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the role of recipient age on the outcome of donor egg cycles. DESIGN:Retrospective cohort study of aggregated national cycles of donor egg therapy that are collected by Society for Assisted Reproductive Technology and the Centers for Disease Control and Prevention. SETTING:Assisted reproductive ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)03371-x
更新日期:2002-11-01 00:00:00
abstract::Modern technologies applied to the field of preimplantation genetic diagnosis for aneuploidy screening (PGD-A) have improved the ability to identify the presence of mosaicism. Consequently, new questions can now be addressed regarding the potential impact of embryo mosaicism on diagnosis accuracy and the feasibility o...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2017.03.019
更新日期:2017-05-01 00:00:00
abstract:OBJECTIVE:To investigate c-fos and matrix metalloproteinase-9 (MMP-9) expression in the endometrium from women with or without endometriosis throughout the menstrual cycle, and to explore the correlation of c-fos expression with MMP-9 expression and 17beta-E(2) levels in serum. DESIGN:Molecular studies in human tissue...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.07.1386
更新日期:2008-10-01 00:00:00
abstract:OBJECTIVE:To investigate the relationship between endogenous serum levels of human growth hormone (hGH) and ovarian response to human menopausal gonadotropins (hMG). DESIGN:Retrospective analysis of patient response to hMG. SETTING:Center for assisted reproductive technology. PATIENTS:Eighty women who had undergone ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)55133-4
更新日期:1992-07-01 00:00:00
abstract:OBJECTIVE:To search for differentially expressed genes in cumulus cells from two groups of oocytes with different developmental outcome in vitro. DESIGN:Analyses of gene expression in human cumulus cells from oocytes that failed to fertilize in vitro (group A) and those that developed into normal-appearing embryos on ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.11.030
更新日期:2005-04-01 00:00:00
abstract::Fifteen subfertile males with absent or atrophic right testes underwent ligation of left varicoceles. Sperm concentration rose significantly with correction of scrotal thermographic abnormality and nine wives (60%) became pregnant. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)55098-5
更新日期:1992-06-01 00:00:00
abstract:OBJECTIVE:To determine whether there is an association between abnormal semen parameters and occupational exposures to organic solvents, metals, and pesticides. DESIGN:Case-control study using three case groups based on different cutoff values for semen parameters and one standard reference group. SETTING:University ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00542-1
更新日期:1999-04-01 00:00:00
abstract:OBJECTIVE:To examine whether a relationship exists between genetic polymorphisms of glutathione S-transferase (GST) M1 and T1, CYP1A1(*)2C, and male factor infertility. DESIGN:Genetic polymorphism analysis, case-control study. SETTING:University research laboratory and andrology clinic. PATIENT(S):One hundred ten me...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.07.017
更新日期:2009-08-01 00:00:00
abstract:OBJECTIVE:To compare the outcomes of intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) with fresh and cryopreserved testicular spermatozoa in patients with obstructive and nonobstructive azoospermia. DESIGN:Retrospective analysis of consecutive ICSI cycles. SETTING:Large urban reproductive medi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(00)00416-7
更新日期:2000-05-01 00:00:00
abstract:OBJECTIVE:To study the controversial association between human leukocyte antigen-G (HLA-G) 14 bp polymorphism and recurrent pregnancy loss (RPL). We performed a meta-analysis of studies in the literature that enrolled only women of European countries who experienced RPL spontaneously or after undergoing IVF. DESIGN:Sy...
journal_title:Fertility and sterility
pub_type: 杂志文章,meta分析
doi:10.1016/j.fertnstert.2019.05.003
更新日期:2019-09-01 00:00:00
abstract::Of 5,341 infertile couples seen over an 8-year period, 76 (1.4%) had primary vaginismus, of whom 63 were treated with the use of a simplified sensate focus technique. There was complete symptomatic resolution of vaginismus in 60 women, and pregnancy was achieved in 33. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.03.071
更新日期:2010-11-01 00:00:00
abstract::Infertile couples who had failed to achieve fertilization of oocytes in previous trials of in vitro fertilization (IVF) were treated by IVF with zona pellucida drilling. Zona drilling entails use of micromanipulation to introduce a gap in the zona pellucida either mechanically or by localized application of a zona sol...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60010-9
更新日期:1988-07-01 00:00:00
abstract::Antiphospholipid syndrome patients with recurrent miscarriage have an impairment in fibrinolysis demonstrated by prolonged clot lysis time (CLT) that cannot be attributed to differences in thrombin activatable fibrinolysis inhibitor (TAFI) antigen levels. Patients with unexplained recurrent miscarriage have an impairm...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.02.032
更新日期:2010-11-01 00:00:00
abstract:OBJECTIVE:To examine the relationship between sperm chromatin defects, evaluated by the flow cytometric (FCM) sperm chromatin structure assay (SCSA), and the probability of a pregnancy in a menstrual cycle (fecundability). DESIGN:Follow-up study. SETTING:The Section of Toxicology and Biomedical Sciences, ENEA Casacci...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(99)00462-8
更新日期:2000-01-01 00:00:00
abstract::In the early years of in vitro fertilization, overall pregnancy rates were low, and it was considered necessary to transfer more than one embryo to increase the chances of pregnancy. It was not until advances in assisted reproductive technologies resulting in increased pregnancy rates that the concept of transferring ...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2020.09.003
更新日期:2020-10-01 00:00:00
abstract::The tenet that a combination of human follicle-stimulating hormone (hFSH)/human menopausal gonadotropin (hMG) improves follicular recruitment was assessed by randomly treating ovulatory women either with hFSH/hMG on days 3 and 4 of the cycle followed by two ampules of hMG daily or with a constant daily dose of 2 ampul...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(16)60315-1
更新日期:1988-11-01 00:00:00
abstract::The authors studied the effects of 4-hydroxyandrostene-3,17-dione (4-OHA) on progesterone (P), 17 beta-estradiol (E2), and 20 alpha-hydroxy-4-pregnen-3-one synthesis and pregnenolone accumulation in cultured human midluteal cells. A dose-dependent inhibition with and without human chorionic gonadotropin (hCG) of E2 an...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54300-3
更新日期:1991-05-01 00:00:00
abstract:OBJECTIVE:To describe a woman with a nonmosaic (45,X) form of Turner's syndrome who gave birth to a girl with 45,X Turner syndrome. DESIGN:Patient report. SETTING:Outpatient clinic of a university hospital. PATIENT(S):A woman with typical phenotypic features of Turner syndrome and a 45,X karyotype and her daughter w...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.02.129
更新日期:2004-10-01 00:00:00
abstract:OBJECTIVE:To evaluate the effectiveness of sublingual misoprostol for cervical ripening before diagnostic hysteroscopy in premenopausal women. DESIGN:Placebo-controlled, double-blind, randomized trial. SETTING:University hospital. PATIENT(S):Fifty-two women with an indication for diagnostic hysteroscopy. INTERVENTI...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2009.01.073
更新日期:2010-05-01 00:00:00
abstract::Twelve human uteri containing intrauterine contraceptive devices (IUDs) and ten uteri without IUDs were obtained at hysterectomy. Samples of fluid were collected from the uterine lumina by absorbing the fluid in small pieces of lens paper. In the samples of luminal fluid we measured the concentration of beta-galactosi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)42078-9
更新日期:1976-09-01 00:00:00
abstract:STUDY OBJECTIVES:To assess a single (donor) sample by the use of five computer-assisted semen analysis (CASA) systems. SETTING:British Andrology Society advanced course on computer-assisted semen analysis techniques, September 1992. PARTICIPANTS:Clinical, technical and scientific personnel with mixed experience of CA...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00