Abstract：
:Infertile couples who had failed to achieve fertilization of oocytes in previous trials of in vitro fertilization (IVF) were treated by IVF with zona pellucida drilling. Zona drilling entails use of micromanipulation to introduce a gap in the zona pellucida either mechanically or by localized application of a zona solvent from a microneedle. Ten couples were treated, from whom 63 oocytes were recovered for manipulation. Sixteen eggs were denuded of the cumulus oophorus only, and the remaining 47 eggs were subjected to zona drilling. Of the 16 eggs denuded but not drilled, 4 (25%) were fertilized. Of the 47 oocytes drilled, 31 survived (67%) and 10 of the surviving eggs (32%) were fertilized. The polyspermy rate for drilled eggs that fertilized was high (5/10, 50%), and polyspermic eggs were often penetrated by more than two spermatozoa. The remaining five eggs fertilized after drilling were diploid fertilizations, and in three cases cleavage was followed by embryo transfer, although pregnancies were not obtained. These data indicate that zona drilling has the potential for establishing pregnancies in instances where treatment by standard IVF would fail. In addition, results indicate that the block to polyspermy in human eggs occurs at the level of the zona pellucida.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Gordon JW,Grunfeld L,Garrisi GJ,Talansky BE,Richards C,Laufer Ndoi
10.1016/s0015-0282(16)60010-9subject
Has Abstractpub_date
1988-07-01 00:00:00pages
68-73issue
1eissn
0015-0282issn
1556-5653pii
S0015-0282(16)60010-9journal_volume
50pub_type
杂志文章abstract：OBJECTIVE:To report the results of 20 orthotopic retransplantations of cryopreserved ovarian tissue after cancer treatment. DESIGN:Retrospective analysis. SETTING:Tertiary gynecology department. PATIENT(S):Twenty patients with malignant disease: 11 with hematological malignancies (55%), four with breast cancer (20%)...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.10.045
更新日期：2015-02-01 00:00:00
abstract：OBJECTIVE:To determine the probability of detecting the gestational sac of a normal intrauterine pregnancy by vaginal ultrasound at different gestational ages and serum hCG concentrations. DESIGN:Prospective, randomized study. SETTING:Pregnant human volunteers in a university-based clinical research environment. PAT...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(16)56749-1
更新日期：1994-06-01 00:00:00
abstract：OBJECTIVE:To determine the effect of human sperm incubation at room temperature (20 degrees C) upon capacitation-related events. DESIGN:Prospective study. SETTING:Basic research laboratory. PATIENT(S):Semen samples were obtained from normozoospermic volunteers. Human follicular fluid (hFF) was collected from women u...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)02982-x
更新日期：2002-02-01 00:00:00
abstract：OBJECTIVE:To test the hypothesis that adrenal androgen (AA) secretion is an inherited trait in PCOS and that serum DHEAS concentrations, as a marker of AA secretion, will be correlated between women with PCOS and their sisters. DESIGN:Prospective case-control. SETTING:Tertiary care center. PATIENT(S):Sixty-two PCOS ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.05.045
更新日期：2006-12-01 00:00:00
abstract：OBJECTIVE:To examine the association between the midfollicular FSH-LH ratio and the number of follicles, and the multifollicular ovarian response to gonadotropin stimulation in patients with polycystic ovary syndrome (PCOS) with normal basal LH and FSH levels. SUBJECT(S):Eighteen patients who had an abandoned treatmen...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)80069-6
更新日期：1997-03-01 00:00:00
abstract：OBJECTIVE:To determine inhibin-B concentrations during ovarian reserve screening in women with normal and diminished ovarian reserve as determined by the clomiphene citrate challenge test. DESIGN:Retrospective. SETTING:Tertiary fertility center. PATIENT(S):Women undergoing ovarian reserve screening for a routine fer...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)00531-1
更新日期：1998-03-01 00:00:00
abstract：OBJECTIVE:To evaluate whether some ultrasound parameters of ovarian morphology can discriminate between control women and patients with polycystic ovary syndrome (PCOS). DESIGN:Retrospective data analysis. SETTING:Volunteers women in an academic research environment. PATIENT(S):Eighty amenorrheic or oligomenorrheic ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)01919-7
更新日期：2001-08-01 00:00:00
abstract：:Effective contraception and easily available abortion have contributed to the decline of adoptable children. This decline has resulted in a marked increase in the demand for AID. The provider of donor semen must establish minimal semen criteria to meet this demand effectively. During the past 8 years the authors have ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)45923-6
更新日期：1981-12-01 00:00:00
abstract：OBJECTIVE:To determine whether men with azoospermia are at an elevated risk of developing cancer in the years following an infertility evaluation. DESIGN:Cohort study. SETTING:United States andrology clinic. PATIENT(S):A total of 2,238 men with complete records were evaluated for infertility at a single andrology cl...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.05.022
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVE:To present four cases of twin tubal pregnancies and discuss possible etiologies. Twin tubal pregnancies are a rare event, with incidence rates estimated as 1 out of 725-1,580 of tubal pregnancies. DESIGN:Case series. SETTING:Department of obstetrics and gynecology at a tertiary health care facility. PATIEN...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.03.017
更新日期：2010-10-01 00:00:00
abstract：OBJECTIVE:To reanalyze the results of using FSH alone and hMG during IVF treatment, taking into account the different protocols of administration of superactive GnRH agonist analogs. DESIGN:Meta-analysis. SETTING:The London Women's Clinic. PATIENT(S):Women undergoing IVF treatment. INTERVENTION(S):A meta-analysis o...
journal_title：Fertility and sterility
pub_type： 杂志文章,meta分析
doi：10.1016/s0015-0282(99)00519-1
更新日期：2000-02-01 00:00:00
abstract：:A recent survey of American Society for Reproductive Medicine (ASRM) members indicates that they express a commitment to professional standards and a desire for advocacy. Their attitudes echo traditional values of professionalism, upon which ASRM should capitalize. ...
journal_title：Fertility and sterility
pub_type： 评论,杂志文章
doi：10.1016/j.fertnstert.2004.01.052
更新日期：2004-09-01 00:00:00
abstract：:The beta-adrenergic agonist isoproterenol was infused intravenously for 4 hours during the midluteal phase of the cycle in six normal women. A rapid increase in cardiac output occurred for the entire duration of the isoproterenol infusion and serum estrogen and progesterone levels (but not luteinizing hormone levels) ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)47712-5
更新日期：1984-03-01 00:00:00
abstract：:To assess the risk of introducing microorganisms into the peritoneal cavity during intracervical or intrauterine insemination, we cultured the cervix and semen from 19 couples before insemination and the peritoneal fluid from the female partner after insemination. The peritoneal cultures taken before hydrotubation gre...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)49458-6
更新日期：1986-07-01 00:00:00
abstract：OBJECTIVE:To analyze the effectiveness of autologous endometrial coculture by the cycle day of the endometrial biopsy. DESIGN:Retrospective study. SETTING:University-based IVF center. PATIENT(S):Two hundred eight patients with multiple IVF failures. INTERVENTION(S):Embryos were split and randomly allocated to growt...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(02)03134-5
更新日期：2002-06-01 00:00:00
abstract：OBJECTIVE:To enable serodiscordant couples to reproduce while minimizing the risk of infecting the female partner and to demonstrate the feasibility of including these couples in an insemination program. DESIGN:Prospective follow-up study. SETTING:Ambulatory IUIs. PATIENT(S):Fifty-six HIV-1-serodiscordant couples wi...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2004.02.128
更新日期：2004-10-01 00:00:00
abstract：:The authors review the current approach to management of the adolescent varicocele which has evolved over the past two decades. Principles of observational, surgical and adjunctive management are discussed relative to significant clinical findings. A selective approach to surgical intervention is advocated with the go...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2011.10.034
更新日期：2011-12-01 00:00:00
abstract：OBJECTIVE:To assess vaginal development, sexual activity, and the efficacy of vaginal dilator therapy in women with vaginal agenesis (Mayer-Rokitansky-Kuster-Hauser syndrome). DESIGN:Retrospective case review. SETTING:Tertiary referral university teaching hospital clinic for disorders of sexual development and differ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.05.060
更新日期：2011-08-01 00:00:00
abstract：OBJECTIVE:To assess the variability of antimüllerian hormone (AMH) concentrations in women with "adequate ovarian reserve" during unstimulated menstrual cycles and to determine the impact on clinical classifications. DESIGN:Pilot cohort study. SETTING:Private fertility clinic. PATIENT(S):Twelve consecutive women (ag...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.01.132
更新日期：2013-05-01 00:00:00
abstract：OBJECTIVE:To develop an experimental model for embryos with a defect specific to hatching, with the purpose of clarifying the mammalian embryo hatching mechanism. DESIGN:A microneedle was inserted under the zona pellucida (ZP) of mouse embryos, and either 1/2 or 1/4 of the blastomeres were mechanically destroyed. The ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)55993-7
更新日期：1993-06-01 00:00:00
abstract：STUDY OBJECTIVES:To assess a single (donor) sample by the use of five computer-assisted semen analysis (CASA) systems. SETTING:British Andrology Society advanced course on computer-assisted semen analysis techniques, September 1992. PARTICIPANTS:Clinical, technical and scientific personnel with mixed experience of CA...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
abstract：:Obesity seems to exert a small but important extraovarian effect that may influence cycle outcome during assisted reproduction. Future investigation should focus on elucidating whether the endometrium is truly responsible for this deficit, and if it is, determining the mechanisms involved. ...
journal_title：Fertility and sterility
pub_type： 评论,杂志文章
doi：10.1016/j.fertnstert.2007.03.079
更新日期：2008-06-01 00:00:00
abstract：OBJECTIVE:To review the literature to determine the magnitude of the effect of 150 micrograms desogestrel-30 micrograms ethinyl E2, an oral contraceptive (OC) formulation, on plasma lipid concentrations in healthy women using meta-analysis techniques. DATA SOURCES:All English-language published reports (1981 to 1991) ...
journal_title：Fertility and sterility
pub_type： 杂志文章,meta分析
doi：
更新日期：1996-06-01 00:00:00
abstract：:The steroidogenic activity of cumulus oophorus granulosa cells obtained from unstimulated rabbits in estrus has been investigated at closely timed intervals in short-term cultures. The presence of viable, structurally well preserved oocytes and the addition of large follicle follicular fluid had no apparent inhibitory...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1975-05-01 00:00:00
abstract：:A single injection of the luteinizing hormone (LH)-releasing hormone (LHRH) agonist [D-Ala6,des-Gly-NH2(10)]LHRH ethylamide to female rats on diestrus I produced a marked reduction in ovarian LH/human chorionic gonadotropin (hCG) and follicle-stimulating hormone (FSH) receptor levels, uterine weight, and plasma proges...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)43524-7
更新日期：1978-09-01 00:00:00
abstract：OBJECTIVE:To design protein phosphatase 1 (PP1)-disrupting peptides covalently coupled to inert cell-penetrating peptides (CPPs) as sychnologically organized bioportide constructs as a strategy to modulate sperm motility. DESIGN:Experimental study. SETTING:Academic research laboratory. PATIENT(S)/ANIMAL(S):Normozoos...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.08.013
更新日期：2020-09-22 00:00:00
abstract：:Three groups of women with different types of ovulatory dysfunction who had failed to conceive on conventional therapy were treated with pulsatile gonadotropin-releasing hormone (GnRH). Group A consisted of nine patients with luteal phase defect; group B included four patients with apparently normal menstrual cycles b...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48971-5
更新日期：1985-11-01 00:00:00
abstract：:In response to large numbers of requests for laparoscopic sterilization, the University of North Carolina began a training program to provide this service on a regional basis throughout the state. This report reviews the final distribution of centers providing these services: one within 50 miles of every person in the...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)42312-5
更新日期：1977-01-01 00:00:00
abstract：OBJECTIVE:To assess the potential involvement of vascular endothelial growth factor in the hyperpermeability characterizing the ovarian hyperstimulation syndrome (OHSS). DESIGN:A controlled clinical study that followed the kinetics of vascular endothelial growth factor in the plasma of patients with severe OHSS from t...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/S0015-0282(97)81908-5
更新日期：1997-02-01 00:00:00
abstract：:To explore the abnormal steroidogenesis in subjects with primary testicular failure, we measured serum levels of testosterone (T) and its precursors androstenedione (delta 4A) and 17-hydroxyprogesterone (17-OHP) in the basal state and after stimulation by human chorionic gonadotropin (hCG) for 4 hours (acute reserve) ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)46401-0
更新日期：1982-07-01 00:00:00
