Abstract:
OBJECTIVE:To search for differentially expressed genes in cumulus cells from two groups of oocytes with different developmental outcome in vitro. DESIGN:Analyses of gene expression in human cumulus cells from oocytes that failed to fertilize in vitro (group A) and those that developed into normal-appearing embryos on day 3 (group B). SETTING:University-based facilities for clinical service and research. PATIENT(S):Women undergoing IVF treatment for infertility. INTERVENTION(S):Cumulus cells were collected from oocytes that were aspirated from ovarian follicles for IVF. The oocytes were cultured individually for IVF and embryo development. Total RNA was extracted from the cumulus cells for gene expression analyses. MAIN OUTCOME MEASURE(S):General gene expression profiles and relative abundance of pentraxin 3 (Ptx3) mRNA. RESULT(S):DNA microarray analysis identified 160 genes, including Ptx3, that were differentially expressed between cumulus cells in group A and group B. Quantitative analysis confirmed that the relative abundance of Ptx3 mRNA in cumulus cells was highly associated with oocyte development. CONCLUSION(S):This study demonstrated that changes in the expression levels of 160 genes, including particularly Ptx3, in human cumulus cells may be indicative of the quality of their enclosed oocyte.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Zhang X,Jafari N,Barnes RB,Confino E,Milad M,Kazer RRdoi
10.1016/j.fertnstert.2004.11.030keywords:
subject
Has Abstractpub_date
2005-04-01 00:00:00pages
1169-79eissn
0015-0282issn
1556-5653pii
S0015-0282(04)03220-0journal_volume
83 Suppl 1pub_type
杂志文章abstract:OBJECTIVE:To evaluate the expression of chemokines that regulate natural killer (NK) and T-regulatory (T-reg) cell activity in eutopic and ectopic endometrial tissue samples from endometriosis patients. DESIGN:Case-control study (Canadian Task Force classification II-2). SETTING:Tertiary referral hospital. PATIENT(S...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.02.038
更新日期:2013-06-01 00:00:00
abstract::Recent observations claimed that growth hormone (GH) administration increased the sensitivity of the ovary to gonadotropin stimulation. These findings prompted us to assess whether ovarian response to human menopausal gonadotropin (hMG) is correlated to GH reserve. Before hMG administration, 25 patients were tested fo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53336-6
更新日期:1990-03-01 00:00:00
abstract::Significant advances have been made in our understanding of ovarian dysfunction in polycystic ovary syndrome (PCOS), and alterations in adipose tissue function are likely to play an important role in its pathophysiology. This review highlights the principal novel concepts presented at the 4th special scientific meetin...
journal_title:Fertility and sterility
pub_type: 共识发展会议,杂志文章,评审
doi:10.1016/j.fertnstert.2009.03.058
更新日期:2010-07-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to assess which of the basal ovarian reserve markers provides the best reflection of the changes occurring in ovarian function over time (i.e., reproductive aging). DESIGN:Prospective longitudinal study. SETTING:Healthy volunteers in an academic research center. PATIENT(S):Eighty-...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.11.029
更新日期:2005-04-01 00:00:00
abstract:OBJECTIVE:To demonstrate the pattern(s) of spermatogonial proliferation in different spermatogenic disorders. DESIGN:Retrospective case-control study. Teaching hospital. PATIENT(S):Azoospermic men who underwent testicular biopsy for sperm recovery and preparation for intracytoplasmic sperm injection. INTERVENTION(S)...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(03)02161-7
更新日期:2003-11-01 00:00:00
abstract:OBJECTIVE:To determine association between health-related quality of life (HRQOL) and psychosexual variables in patients with polycystic ovary syndrome (PCOS). DESIGN:Cross-sectional study. SETTING:Two private gynecology clinics. PATIENT(S):A sample of 300 women with PCOS were entered into the study and were subdivi...
journal_title:Fertility and sterility
pub_type: 杂志文章,多中心研究
doi:10.1016/j.fertnstert.2013.06.043
更新日期:2013-11-01 00:00:00
abstract::Suppression of the pituitary-gonadal axis by the administration of gonadotropin-releasing hormone agonists (GnRH-a) has been used for a variety of endocrinological and gynecological disorders. The suppressive effect of GnRH-a on luteinizing hormone, follicle-stimulating hormone, and sex steroid production is well docu...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53639-5
更新日期:1990-07-01 00:00:00
abstract:OBJECTIVE:To investigate whether temporarily withholding FSH and adding androgen could improve follicular response during a microdose flare protocol in women with slow follicular growth or asynchronous follicular development. DESIGN:Observational pilot study. SETTING:University-affiliated private fertility center. P...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2015.09.038
更新日期:2016-01-01 00:00:00
abstract::Examination of 12 paired peritoneal ectopic and eutopic endometria for histochemical binding of Dolichos biflorus agglutinin, normally found in the mid-late secretory part of the cycle, showed a failure of lectin binding in 9 of 11 secretory-phase lesions although the eutopic specimens generally stained normally. This...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.11.032
更新日期:2009-04-01 00:00:00
abstract:OBJECTIVE:To explore the effectiveness of percutaneous vasal sperm aspiration (PVSA) in combination with intrauterine insemination (IUI) to treat infertile men with anejaculation. DESIGN:Clinical study. SETTING:Department of reproductive endocrinology and andrology of a family planning research clinic. PATIENT(S):Tw...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)04697-6
更新日期:2003-03-01 00:00:00
abstract::The effect of administration of a superactive and long-acting analog of luteinizing hormone-releasing hormone (LH-RH), D-Trp6-LH-RH, in doses of 0.05 or 1 microgram/day for 10 days on the hypothalamic-pituitary-gonadal axis was studied in immature female rats. Treatment with a 0.05-microgram dose of analog produced fe...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)44061-6
更新日期:1979-06-01 00:00:00
abstract:OBJECTIVE:To evaluate the use of sonohysterography for uterine screening before IVF. DESIGN:Prospective screening with sonohysterography and comparison with available hysterosalpingographic and hysteroscopic evaluations. SETTING:Private practice. PATIENT(S):Seventy-two women undergoing IVF-ET using their own or dono...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00045-4
更新日期:1998-05-01 00:00:00
abstract:OBJECTIVE:To study the oocyte quality in patients with ovarian hyperstimulation syndrome (OHSS). DESIGN:Retrospective study. SETTING:The Egyptian IVF-ET Center. PATIENT(S):Forty-two patients who developed severe OHSS (group A) were studied for the mean number of oocytes retrieved, percentage of high-quality oocytes,...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(97)00409-3
更新日期:1997-12-01 00:00:00
abstract:OBJECTIVE:To evaluate the consistency and objectivity of deep neural networks in embryo scoring and making disposition decisions for biopsy and cryopreservation in comparison to grading by highly trained embryologists. DESIGN:Prospective double-blind study using retrospective data. SETTING:U.S.-based large academic f...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2019.12.004
更新日期:2020-04-01 00:00:00
abstract::Daily early morning urine samples were collected from women receiving donor artificial insemination and were evaluated for estrone conjugates, pregnanediol-3-glucuronide, and human chorionic gonadotropin. All hormone concentrations in urine were indexed by creatinine levels and expressed as the concentration of hormon...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48613-9
更新日期:1985-06-01 00:00:00
abstract:OBJECTIVE:To study the changes in semen parameters, gonadotropic and sex hormones, and serum leptin in obese fertile and infertile oligozoospermic men. DESIGN:Prospective. SETTING:University hospital. PATIENT(S):Forty-two obese fertile and 80 obese infertile oligozoospermic men (body mass index [BMI] >30). INTERVEN...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.03.085
更新日期:2010-07-01 00:00:00
abstract:OBJECTIVE:To determine whether NR5A1 (SF-1) variants are a cause of primary ovarian insufficiency (POI) in 26 young women with similar genetic background. DESIGN:Genetic and functional mutation study. SETTING:University hospitals. PATIENT(S):Genetic analysis of the NR5A1 gene in 26 XX girls with POI. INTERVENTION(S...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2012.10.026
更新日期:2013-02-01 00:00:00
abstract:OBJECTIVE:To compare the oocyte-activating ability of whole human round spermatids and their isolated nuclei. DESIGN:Prospective study using sibling oocytes from patients undergoing spermatid conception and intracytoplasmic sperm injection treatment cycles. SETTING:Private assisted reproduction laboratories and a uni...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(00)01598-3
更新日期:2000-12-01 00:00:00
abstract::Eighteen premenopausal women with symptomatic leiomyomata uteri were enrolled in a stratified, randomized, double-blind, placebo-controlled study evaluating the efficacy of leuprolide acetate (LA) depot treatment before myomectomy. Stratification was based on pretreatment uterine volume (less than 600 cm3 versus great...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1989-11-01 00:00:00
abstract:OBJECTIVE:To investigate the possibility of genetic contribution of CYP1A1, CYP19, GSTM1, and GSTT1 polymorphisms to endometriosis. DESIGN:Genetic polymorphism analysis. SETTING:Case-control study. PATIENT(S):A group of 275 women with sporadic endometriosis was compared with a group of 346 fertile, endometriosis-fre...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)04817-3
更新日期:2003-03-01 00:00:00
abstract:OBJECTIVE:To test the efficacy of endometrial preparation with exogenous steroids, without pretreatment with gonadotropin-releasing hormone (GnRH) agonist, in women with normal ovarian function. DESIGN:Prospective randomized study. SETTING:Private outpatient infertility clinic. PATIENT(S):Two hundred ninety-six wome...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(02)02960-6
更新日期:2002-05-01 00:00:00
abstract::Two-cell mouse embryos were cultured in Ham's F-10 medium (Gibco, Grand Island, NY) supplemented with various concentrations of transferrin, insulin, epidermal growth factor, platelet-derived growth factor, or fibroblast growth factor. Rates of development and the cell numbers per blastocyst were compared between embr...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54082-5
更新日期:1991-01-01 00:00:00
abstract:OBJECTIVE:To determine whether men with azoospermia are at an elevated risk of developing cancer in the years following an infertility evaluation. DESIGN:Cohort study. SETTING:United States andrology clinic. PATIENT(S):A total of 2,238 men with complete records were evaluated for infertility at a single andrology cl...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.05.022
更新日期:2013-09-01 00:00:00
abstract:OBJECTIVE:To demonstrate the laparoscopic neuroanatomy of the autonomic nerves of the pelvis using the laparoscopic neuronavigation technique, as well as the technique for a nerve-sparing radical endometriosis surgery. DESIGN:Step-by-step explanation of the technique using videos and pictures (educational video) to de...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2015.07.1138
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVE:To determine the optimal GnRH agonist dose for triggering of oocyte maturation in oocyte donors. DESIGN:Single-center, randomized, parallel, investigator-blinded trial. SETTING:IVFMD, My Duc Hospital, Ho Chi Minh City, Vietnam. PATIENT(S):One hundred sixty-five oocyte donors (aged 18-35 years, body mass in...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2015.10.014
更新日期:2016-02-01 00:00:00
abstract::We have microsurgically extracted a pronucleus from 11 tripronuclear human zygotes under two different experimental conditions. One group of six zygotes was incubated in cytochalasin D for 30 minutes before manipulation. The remaining five zygotes were manipulated without preincubation in cytoskeletal relaxing agents....
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60901-9
更新日期:1989-09-01 00:00:00
abstract:OBJECTIVE:To evaluate the safety profile of endometrial ablation performed with a thermal balloon as defined by serosal temperature elevation and depth of injury. DESIGN:Observational study with histopathologic correlation conducted in ex vivo and in vivo phases. SETTING:Academic medical center. PATIENT(S):Twenty pa...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00245-3
更新日期:1998-10-01 00:00:00
abstract:OBJECTIVE:To study the daily levels of salivary progesterone (P) during the menstrual cycles of adolescent girls 14 to 19 years of age and the occurrence of anovulations and defective luteal phases. DESIGN:The salivary P levels were analyzed by radioimmunoassay from three age groups of girls (n = 65) and compared with...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::This monograph, written by the pioneers of IVF and reproductive medicine, celebrates the history, achievements, and medical advancements made over the last 40 years in this rapidly growing field. ...
journal_title:Fertility and sterility
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.fertnstert.2018.06.005
更新日期:2018-07-15 00:00:00
abstract:OBJECTIVE:To describe two cases of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) with testicular sperm in men with immotile cilia syndromes. DESIGN:Case report. SETTING:A university-based male infertility clinic and assisted reproduction unit. PATIENT(S):Two couples with male factor infert...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(01)01974-4
更新日期:2001-09-01 00:00:00