Placental expression profiling in preeclampsia: local overproduction of hemoglobin may drive pathological changes.

Abstract:

OBJECTIVE:To create a library enriched in cDNAs from preeclamptic placentas to print onto microarrays for placental profiling of preeclampsia (PE) and high risk pregnancies. DESIGN:Prospective study. SETTING:University women's clinic and academic research laboratory. PATIENT(S):Ten patients with PE, 5 with PE and bilateral notching, 5 with bilateral notching without PE, and 15 normotensive patients were recruited. INTERVENTION(S):Placenta and placenta bed biopsies were collected after delivery. MAIN OUTCOME MEASURE(S):Subtracted libraries of PE transcripts were produced, and cDNAs from these libraries were used to make PE-specific cDNA arrays. Results were verified quantitatively using real-time polymerase chain reaction (PCR) and histologically using in situ hybridization and immunohistochemistry. RESULT(S):Thirty genes were significantly altered in at least one group comparison. Differences in two candidate genes were confirmed using quantitative real-time PCR. Hemoglobin alpha2 and gamma transcripts were significantly overexpressed in the PE placenta. Scattered cells in the placenta and placental blood vessels were shown to express genes encoding these hemoglobin chains. CONCLUSION(S):We demonstrate increased hemoglobin production in the PE placenta. The hemoglobin may be released into the placenta blood vessel lumen. Free heme and hemoglobin are potent toxins that cause endothelial damage and inflammation.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Centlow M,Carninci P,Nemeth K,Mezey E,Brownstein M,Hansson SR

doi

10.1016/j.fertnstert.2007.09.030

subject

Has Abstract

pub_date

2008-11-01 00:00:00

pages

1834-43

issue

5

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(07)03653-9

journal_volume

90

pub_type

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