Induction of the stellate configuration in cultured iris epithelial cells by adenosine and compounds related to adenosine 3':5'-cyclic monophosphate.

abstract：:The heptadecapeptide orphanin FQ (OFQ) is a recently discovered neuropeptide that exhibits structural features reminiscent of the opioid peptides and that is an endogenous ligand to a G protein-coupled receptor sequentially related to the opioid receptors. We have cloned both the human and rat cDNAs encoding the OFQ p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nothacker HP,Reinscheid RK,Mansour A,Henningsen RA,Ardati A,Monsma FJ Jr,Watson SJ,Civelli O

更新日期：1996-08-06 00:00:00

abstract：:The coexistence of two phylogenetically distinct symbiont species within a single cell, a condition not previously known in any metazoan, is demonstrated in the gills of a Mid-Atlantic Ridge hydrothermal vent mussel (family Mytilidae). Large and small symbiont morphotypes within the gill bacteriocytes are shown to be ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Distel DL,Lee HK,Cavanaugh CM

更新日期：1995-10-10 00:00:00

abstract：:Aptamers recognize their targets with extraordinary affinity and specificity. The aptamer-based therapeutic, Macugen, is derived from a modified 2'fluoro pyrimidine RNA inhibitor to vascular endothelial growth factor (VEGF) and is now being used to treat the wet form of age-related macular degeneration. This VEGF(165)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee JH,Canny MD,De Erkenez A,Krilleke D,Ng YS,Shima DT,Pardi A,Jucker F

更新日期：2005-12-27 00:00:00

abstract：:Ciliary Neurotrophic Factor (CNTF) was first characterized as a trophic factor for motor neurons in the ciliary ganglion and spinal cord, leading to its evaluation in humans suffering from motor neuron disease. In these trials, CNTF caused unexpected and substantial weight loss, raising concerns that it might produce ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lambert PD,Anderson KD,Sleeman MW,Wong V,Tan J,Hijarunguru A,Corcoran TL,Murray JD,Thabet KE,Yancopoulos GD,Wiegand SJ

更新日期：2001-04-10 00:00:00

abstract：:125I-Labeled L-fucose-albumin complex and rat preputial beta-glucuronidase are rapidly cleared from plasma after intravenous infusion. L-Fucose-albumin retards the plasma clearance of beta-glucuronidase whereas D-fucose-albumin is inactive. In vitro, 125I-labeled L-fucose-albumin is taken up into rat or rabbit alveola...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shepherd VL,Lee YC,Schlesinger PH,Stahl PD

更新日期：1981-02-01 00:00:00

abstract：:Biological resurfacing of entire articular surfaces represents an important but challenging strategy for treatment of cartilage degeneration that occurs in osteoarthritis. Not only does this approach require anatomically sized and functional engineered cartilage, but the inflammatory environment within an arthritic jo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moutos FT,Glass KA,Compton SA,Ross AK,Gersbach CA,Guilak F,Estes BT

更新日期：2016-08-02 00:00:00

abstract：:Preaxial polydactyly (PPD) is a common limb malformation in human. A number of polydactylous mouse mutants indicate that misexpression of Shh is a common requirement for generating extra digits. Here we identify a translocation breakpoint in a PPD patient and a transgenic insertion site in the polydactylous mouse muta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lettice LA,Horikoshi T,Heaney SJ,van Baren MJ,van der Linde HC,Breedveld GJ,Joosse M,Akarsu N,Oostra BA,Endo N,Shibata M,Suzuki M,Takahashi E,Shinka T,Nakahori Y,Ayusawa D,Nakabayashi K,Scherer SW,Heutink P,Hill RE

更新日期：2002-05-28 00:00:00

abstract：:The conformation of L-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) has been derived from electron-density maps calculated at 2.8-A resolution with phases obtained from two heavy-atom derivatives and the bound coenzyme, NAD. Like other dehydrogenases, 3-hydroxyacyl-CoA dehydrogenase is a double-domain structure, but t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Birktoft JJ,Holden HM,Hamlin R,Xuong NH,Banaszak LJ

更新日期：1987-12-01 00:00:00

abstract：:The fate of two recently described human B lymphocyte-specific antigens (B1 and B2) was studied after B-cell activation in vivo and in vitro. Whereas both B1 and B2 were present on virtually all B cells from normal lymph nodes, B2 was absent from approximately 50% of B cells from hyperplastic lymph nodes. When B cells...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stashenko P,Nadler LM,Hardy R,Schlossman SF

更新日期：1981-06-01 00:00:00

abstract：:Studies by several investigators have shown that 12-0-tetradecanoylphorbol-13-acetate (TPA) is an extraordinarily potent stimulator of differentiation of cultured human promyelocytic leukemia cells in vitro. In the present study, TPA was administered to humans by i.v. infusion without irreversible toxicity, and it was...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han ZT,Zhu XX,Yang RY,Sun JZ,Tian GF,Liu XJ,Cao GS,Newmark HL,Conney AH,Chang RL

更新日期：1998-04-28 00:00:00

abstract：:Clostridium perfringens iota-toxin (Ia) mono-ADP ribosylates Arg177 of actin, leading to cytoskeletal disorganization and cell death. To fully understand the reaction mechanism of arginine-specific mono-ADP ribosyl transferase, the structure of the toxin-substrate protein complex must be characterized. Recently, we so...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsurumura T,Tsumori Y,Qiu H,Oda M,Sakurai J,Nagahama M,Tsuge H

更新日期：2013-03-12 00:00:00

abstract：:Much debate has revolved around the question of whether the mode of evolutionary and ecological turnover in the fossil record of African mammals was continuous or pulsed, and the degree to which faunal turnover tracked changes in global climate. Here, we assembled and analyzed large specimen databases of the fossil re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bibi F,Kiessling W

更新日期：2015-08-25 00:00:00

abstract：:As 3D culture has become central to investigation of tissue biology, mammary epithelial organoids have emerged as powerful tools for investigation of epithelial cell polarization and carcinogenesis. However, most current protocols start from single cells suspended in Matrigel, which can also restrict cell differentiat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Florian S,Iwamoto Y,Coughlin M,Weissleder R,Mitchison TJ

更新日期：2019-06-04 00:00:00

abstract：:We have studied the genetic interactions between mutant alleles in 12 genes, designated PER1-PER12, which are essential for peroxisome biogenesis in the yeast Hansenula polymorpha. Recessive mutations in any of these genes determined three different morphological phenotypes: (i) complete absence of peroxisomes (Per-);...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Titorenko VI,Waterham HR,Cregg JM,Harder W,Veenhuis M

更新日期：1993-08-15 00:00:00

abstract：:Isolated yeast mitochondria, which synthesize identifiable polypeptides identical to those made in vivo, have been used in an invitro system to study cytoplasmic control of mitochondrial protein synthesis. It has been found that protein synthesis in isolated mitochondria is dependent on an endogenous pool of cytoplasm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poyton RO,Kavanagh J

更新日期：1976-11-01 00:00:00

abstract：:A varying oxygen environment is known to affect cellular function in disease as well as activity of various therapeutics. For transient structures, whether they are unconstrained therapeutic transplants, migrating cells during tumor metastasis, or cell populations induced by an immunological response, the role of oxyg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spanoudaki V,Doloff JC,Huang W,Norcross SR,Farah S,Langer R,Anderson DG

更新日期：2019-03-12 00:00:00

abstract：:The triphenylmethane derivative aurintricarboxylic acid (ATA), but not aurin, selectively prevented the binding of OKT4A/Leu-3a monoclonal antibody (mAb) and, to a lesser extent, OKT4 mAb to the CD4 cell receptor for human immunodeficiency virus type 1 (HIV-1). The effect was seen within 1 min at an ATA concentration ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schols D,Baba M,Pauwels R,Desmyter J,De Clercq E

更新日期：1989-05-01 00:00:00

abstract：:Galectin-3 is a glycan-binding protein (GBP) that binds β-galactoside glycan structures to orchestrate a variety of important biological events, including the activation of hepatic stellate cells and regulation of immune responses. While the requisite glycan epitopes needed to bind galectin-3 have long been elucidated...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Joeh E,O'Leary T,Li W,Hawkins R,Hung JR,Parker CG,Huang ML

更新日期：2020-11-03 00:00:00

abstract：:An equation for the bond angles OC-M-CO for tetracarbonyl groups in which the transition metal atom M is enneacovalent, derived from the simple theory of hybrid sp(3)d(5) bond orbitals, is tested by comparison of the calculated values of the angles with the experimental values reported for many compounds containing M(...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pauling L

更新日期：1978-02-01 00:00:00

abstract：:Although de novo protein design is an important endeavor with implications for understanding protein folding, until now, structures have been determined for only a few 25- to 30-residue designed miniproteins. Here, the NMR solution structure of a complex 73-residue three-helix bundle protein, alpha3D, is reported. The...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walsh ST,Cheng H,Bryson JW,Roder H,DeGrado WF

更新日期：1999-05-11 00:00:00

abstract：:Pressures of 200-1000 atmospheres (20-100 MPa) are found to decrease significantly the amount of phosphate (H32PO4-2)transported into the rabbit erythrocyte under otherwise physiological conditions. Such compression does not cause irreversible morphological changes or result in membrane disruption. The findings are in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murphy RB,Libby WF

更新日期：1976-08-01 00:00:00

abstract：:Homeobox 9 (HOXB9), a nontransforming transcription factor overexpressed in breast cancer, alters tumor cell fate and promotes tumor progression and metastasis. Here we show that HOXB9 confers resistance to ionizing radiation by promoting DNA damage response. In nonirradiated cells, HOXB9 induces spontaneous DNA damag...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chiba N,Comaills V,Shiotani B,Takahashi F,Shimada T,Tajima K,Winokur D,Hayashida T,Willers H,Brachtel E,Vivanco MD,Haber DA,Zou L,Maheswaran S

更新日期：2012-02-21 00:00:00

abstract：:Underacetylation of histone H4 is thought to be involved in the molecular mechanism of mammalian X chromosome inactivation, which is an important model system for large-scale genetic control in eukaryotes. However, it has not been established whether histone underacetylation plays a critical role in the multistep inac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wakefield MJ,Keohane AM,Turner BM,Graves JA

更新日期：1997-09-02 00:00:00

abstract：:Leukemia cell lines of the monocytic series (HL-60, U-937, and P388D1) produce a hepatocyte-stimulating factor (HSF) following induction of differentiation with phorbol diester. In 24-72 hr, these leukemia cells produce 2-30% the amount of HSF as human peripheral blood monocytes. Cells of the series at earlier stages ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woloski BM,Fuller GM

更新日期：1985-03-01 00:00:00

abstract：:LINEs are transposable elements found in various eukaryotes such as plants, protists, insects, and mammals. Their transposition is usually difficult to study, particularly in humans, where some diseases have been shown to result from LINE insertion mutations. This is due to the fact that most copies of any particular ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pélisson A,Finnegan DJ,Bucheton A

更新日期：1991-06-01 00:00:00

abstract：:Intermolecular electronic coupling dictates the optical properties of molecular aggregate systems. Of particular interest are photosynthetic pigment-protein complexes that absorb sunlight then efficiently direct energy toward the photosynthetic reaction center. Two-dimensional (2D) ultrafast spectroscopy has been used...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Read EL,Engel GS,Calhoun TR,Mancal T,Ahn TK,Blankenship RE,Fleming GR

更新日期：2007-09-04 00:00:00

abstract：:Molecular modeling and two-dimensional NMR techniques enable us to identify structural features in the third variable region (V3) loop of the human immunodeficiency virus (HIV) surface glycoprotein gp120, in particular the principal neutralizing determinant (PND), that remain conserved despite the sequence variation. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fontenot JD,Gatewood JM,Mariappan SV,Pau CP,Parekh BS,George JR,Gupta G

更新日期：1995-01-03 00:00:00

abstract：:Transcription in eukaryotic nuclei is carried out by DNA-dependent RNA polymerases I, II, and III. Human RNA polymerase III (Pol III) transcribes small untranslated RNAs that include tRNAs, 5S RNA, U6 RNA, and some microRNAs. Increased Pol III transcription has been reported to accompany or cause cell transformation. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haurie V,Durrieu-Gaillard S,Dumay-Odelot H,Da Silva D,Rey C,Prochazkova M,Roeder RG,Besser D,Teichmann M

更新日期：2010-03-02 00:00:00

abstract：:The torI gene has been identified by using a genetic multicopy approach as a negative regulator of the torCAD operon that encodes the trimethylamine N-oxide reductase respiratory system in Escherichia coli. The negative effect was due to a previously unidentified small ORF (66 aa) of phage origin that we called torI f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ansaldi M,Théraulaz L,Méjean V

更新日期：2004-06-22 00:00:00

abstract：:Phage phi 29 DNA cannot be phosphorylated with polynucleotide kinase and [gamma-32P]ATP because of the presence of a viral protein covalently linked to the 5' termini. The 5' ends can, however, be made susceptible to phosphorylation by treatment with alkali and alkaline phosphatase. Restriction fragments Hpa II C and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Escarmís C,Salas M

更新日期：1981-03-01 00:00:00