Abstract:
:Western blotting was used as a powerful alternative to immunoprecipitation for the detection of the simian virus 40 (SV40) large tumor (T) antigen. After resolution by electrophoresis on a SDS-polyacrylamide gel of a [35S]methionine labeled crude extract from SV40 infected monkey kidney cells, the separated proteins were transferred electrophoretically on nitrocellulose paper. T antigen was detected on nitrocellulose strips by using for the first time, specific, purified antipeptide monoclonal antibodies directed against the N- and C-terminal portions of the molecule, and 125I-labeled Protein A.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Dietrich JBdoi
10.1007/BF01117060subject
Has Abstractpub_date
1985-02-01 00:00:00pages
137-42issue
2eissn
0144-8463issn
1573-4935journal_volume
5pub_type
杂志文章abstract::This review focuses on the recent developments in the study of drug interactions with biological membranes and membrane-associated proteins using nuclear magnetic resonance (NMR) spectroscopy and other spectroscopic techniques. Emphasis is placed on a class of low-affinity neurological agents as exemplified by volatil...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1023/a:1020182404940
更新日期:2002-04-01 00:00:00
abstract::Since the Protein Data Bank (PDB) was founded in 1971, there are now over 120,000 depositions, the majority of which are from X-ray crystallography and 90% of those made use of synchrotron beamlines. At the Cambridge Structure Database (CSD), founded in 1965, there are more than 800,000 'small molecule' crystal struct...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20170204
更新日期:2017-07-04 00:00:00
abstract::The effect of carbamylcholine and the calcium ionophore A23187 on catecholamine release and intracellular free calcium, [Ca2+]i, in bovine adrenal chromaffin cells was determined. At 10(-4) M carbamylcholine maximal release occurred with an accompanying increase in [Ca2+]i from a basal level of 168 nM to less than 300...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121918
更新日期:1984-07-01 00:00:00
abstract:AIM:To explore the prognostic value of clusterin (CLU) in hepatocellular carcinoma (HCC) patients treated with oxaliplatin (OXA). METHODS:Relative expression of plasma CLU mRNA was examined via fluorescence quantitative real-time PCR (qRT-PCR), and CLU protein level in tissue samples was detected through immunohistoch...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20200071
更新日期:2020-02-28 00:00:00
abstract::Using a quantitative enzyme immunoassay, Thy-1 antigen expressed by a rat myoid cell line R615B2 was detected mainly on the cell surface at a single cell stage, whereas at the stage of forming myotubes, Thy-1 was found predominantly in the cytoplasm. The muscle specific creatine kinase activity also increased in assoc...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119804
更新日期:1989-10-01 00:00:00
abstract:AIM:IgA nephropathy (IgAN) is one of the most common chronic glomerulonephritis. Its etiology and pathogenesis remain unclear. We thus explored the immune repertoire of the B-cell receptor (BCR) and the heavy-chain complementarity-determining region 3 (CDR3) in renal tissue and peripheral blood of IgAN patients. METHO...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190482
更新日期:2019-10-30 00:00:00
abstract::Due to poor prognosis of glioblastoma (GBM), there is an urgent need to develop new therapeutic strategies. Besides eliminating GBM tumor cells and stem cells, a novel therapeutic approach aims to target Glioma-associated microglia/macrophages (GAMs). We investigated the molecular profile of GAMs correlated with patie...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20182361
更新日期:2019-06-20 00:00:00
abstract::Leishmaniasis is a group of infectious and non-contagious severe parasitic diseases, caused by protozoans of the Leishmania genus. Natural products characterize a rich source of prospective chemical entities for the development of new effective drugs for neglected diseases. Scientific evaluation of medicinal plants ha...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20202672
更新日期:2020-11-30 00:00:00
abstract::Citrullus lanatus (Cucurbitaceae) is conventionally used for the treatment of urinary tract infection, renal stones, hypertension, diabetes and diarrhoea. Current study evaluates acute and 28 days repeated toxicity ethanolic extract of C. lanatus seed (EECLS) in Wistar rats to measure its safety profile. The single do...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20202345
更新日期:2021-01-29 00:00:00
abstract::Heterotopic ossification (HO) is the aberrant formation of mature, lamellar bone in nonosseous tissue. Fibrodysplasia ossificans progressiva (FOP) is a rare and devastating genetic disorder that causes progressive HO in the ligaments, tendons, and muscles throughout the body. FOP is attributed to an autosomal mutation...
journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20190377
更新日期:2019-08-02 00:00:00
abstract::Antimicrobial peptides are a promising resource for developing novel antibiotic and even anticancer drugs. Here, a 28-mer polypeptide, Ranatuerin-2PLx (R2PLx), was identified from lyophilised skin secretions. The chemically synthetic replicates exhibited moderate and broadspectrum antimicrobial effect against various ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20180710
更新日期:2018-11-09 00:00:00
abstract::Common colds were shortened by 7 days in a 1984 clinical trial using zinc gluconate throat lozenges each 2 h. Between then and 2004, 10 other double-blind, placebo-controlled clinical trials showed widely varying results. This re-analysis of these trials presents solution chemistry methods to elucidate differences in ...
journal_title:Bioscience reports
pub_type: 杂志文章,meta分析
doi:10.1023/b:bire.0000037754.71063.41
更新日期:2004-02-01 00:00:00
abstract::Rabbit reticulocyte lysates, gel filtered on Sephadex G-25 with or without ATP (or its analogs), were preincubated at 37 degrees C and their subsequent binding to p3A4,3'-[32P]pCp was studied. Lysates filtered without ATP or in the presence of 0.1 mM 8-bromo-ATP, 1,N6-etheno-ATP, or ITP showed a time-dependent decreas...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01119625
更新日期:1985-12-01 00:00:00
abstract::Recent experimental evidence increasingly shows that the dysregulation of cellular bioenergetics is associated with a wide array of common human diseases, including cancer, neurological diseases and diabetes. Respiration provides a vital source of cellular energy for most eukaryotic cells, particularly high energy dem...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160083
更新日期:2016-06-30 00:00:00
abstract::Native bovine parathyroid hormone (bPTH) was found to be readily cleaved with human leukocyte elastase to yield the fragments bPTH(1-41) and bPTH(42-84). These were then isolated by reverse-phase HPLC and characterised by gas-phase sequencing and amino acid analysis. The biological activities of these fragments were a...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122726
更新日期:1987-01-01 00:00:00
abstract::Mouse Mex3c encodes RNA-binding proteins of variant length through alternative splicing. Its mutation results in multiple defects including growth retardation, perturbed energy balance, and defective antiviral innate immunity. Here we report that Mex3c mutation affects mammary gland development and lactation in female...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201285
更新日期:2020-12-23 00:00:00
abstract::Mice and rats are animals commonly used in research and laboratory testing. Compared with other animal species, they harbor many more zoonotic agents. Hymenolepis nana (H. nana) is a common tapeworm that parasitizes both humans and rodents. Although this tapeworm is of socio-economic importance worldwide, information ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181224
更新日期:2019-02-08 00:00:00
abstract::Objectives In the present study, we examined available articles from online databases to comprehensively investigate the effect of the XPC (xeroderma pigmentosum complementation group C) rs2228000 polymorphism on the risk of different types of clinical cancer. Methods We conducted a group of overall and subgroup pooli...
journal_title:Bioscience reports
pub_type: 杂志文章,meta分析
doi:10.1042/BSR20192452
更新日期:2019-12-20 00:00:00
abstract::The ubiquitin-proteasome system (UPS)-dependent proteolysis plays a major role in the muscle catabolic action of glucocorticoids (GCs). Atrogin-1 and muscle-specific RING finger protein 1 (MuRF1), two E3 ubiquitin ligases, are uniquely expressed in muscle. It has been previously demonstrated that GC treatment induced ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171056
更新日期:2017-11-17 00:00:00
abstract::Superoxide dismutase (SOD), a metal containing enzyme is present in parasite Leishmania donovani as well as in host macrophages both resident and activated in a detectable amount, although the level is much higher in the latter case. It is observed that at any particular protein concentration, the SOD activity is high...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116457
更新日期:1988-04-01 00:00:00
abstract::Microparticles (MPs) and miRNAs have been shown to play important roles in coronary artery disease (CAD) by monitoring endothelial dysfunction. The present study aims to investigate the diagnostic value of endothelial MPs (EMPs) and miRNAs (miR-92a or miR-23a) as biomarkers in distinguishing patients with acute myocar...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170047
更新日期:2017-03-27 00:00:00
abstract::Poly(A)+RNA was isolated from brown adipose tissue of cold acclimated rats and a fraction enriched for uncoupling protein mRNA was used to generate a cDNA library in pBR 322. Immunological screening of 1,500 colonies with an affinity-purified antiserum against the uncoupling protein yielded five positive clones, pUCPr...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01145183
更新日期:1986-01-01 00:00:00
abstract::The preadipocyte differentiation biological process involves a cascade of transcriptional events that culminates in the expression of peroxisome proliferator-activated receptor (PPAR) γ. The differentiation cocktail [insulin (INS), dexamethasone (DEX) and isobutylmethylxanthine (IBMX)] can induce preadipocyte differen...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160049
更新日期:2016-11-03 00:00:00
abstract::The concentration of Na+ and K+ and the pH in the cytoplasm of Lettré cells was measured by monitoring the net flux of H+, Na+, or K+ across the plasma membrane which had been rendered permeable to these ions by the action of Sendai virus. Ion flux was measured directly by analysis of cell composition, or indirectly b...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01172873
更新日期:1983-07-01 00:00:00
abstract::The purpose of the study is to investigate the correlation between the expression of C-reactive protein (CRP) and autophagy-related 9B (ATG9B) and pathological features of clear cell renal cell carcinoma (CCRCC) patients. We also intended to explore the effects of manipulated expression of CRP and ATG9B on the apoptos...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20171082
更新日期:2017-11-15 00:00:00
abstract::In the present study, we aimed to examine whether SET domain-containing methyltransferases are up-regulated in different classes of renal cell carcinoma. We immunoblotted against SET domain and quantified the expression of these modular domains. Furthermore, we examined the expression of Rad51, the key protein that co...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160122
更新日期:2016-06-30 00:00:00
abstract::To observe the effect of gene expression and tumorigenicity in hybrid cells of human embryonic stem cells (hESCs) and ovarian cancer cells in vitro and in vivo using a mouse model, and to determine its feasibility in reprogramming tumour cells growth and apoptosis, for a potential exploration of the role of hESCs and ...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20160104
更新日期:2016-09-16 00:00:00
abstract::eNOS (endothelial nitric oxide synthase) contains a MAPK (mitogen-activated protein kinase)-binding site associated with a major eNOS control element. Purified ERK (extracellular-signal-regulated kinase) phosphorylates eNOS with a stoichiometry of 2-3 phosphates per eNOS monomer. Phosphorylation decreases NO synthesis...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20140015
更新日期:2014-09-17 00:00:00
abstract::We aimed to confirm the role of miR-1296-5p in gastric cancer and to identify its target genes. The expression of miR-1296-5p was measured in gastric cancer tissues and cell lines. The function of miR-1296-5p was examined by the overexpression and inhibition of its expression in typical gastric cell lines as well as S...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181556
更新日期:2019-01-08 00:00:00
abstract::LncRNA PACER is a chondrocyte inflammation-associated long non-coding RNA (lncRNA), and chondrocyte inflammation is involved in osteoarthritis (OA). We observed that plasma PACER was down-regulated, while plasma HOTAIR was up-regulated in OA patients. Altered plasma levels of PACER and HOTAIR distinguished OA patients...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190404
更新日期:2019-06-07 00:00:00