Abstract：
:The distribution of actin, alpha-actinin, fimbrin, tropomyosin and tubulin in the apical region of inner and outer hair cells was studied by immunofluorescent localization of antibodies to these proteins. The macromolecular distribution of actin and alpha-actinin was studied using post-embedding immunoelectron microscopic techniques. Actin is present in the stereocilia and cuticular plate of both inner and outer hair cells. Antibodies to actin were localized with fluorescence and colloidal gold. Colloidal gold particles were distributed uniformly over the stereocilia, stereocilia rootlets and cuticular plate. Fimbrin is present in the stereocilia and the cuticular plate. Immunofluorescent label was more intense over the cuticular plate of outer hair cells than over the cuticular plate of inner hair cells. Alpha-actinin is present in the cuticular plate only. At the ultrastructural level, antibodies to alpha-actinin were labeled throughout the cuticular plate, with larger accumulations of colloidal gold over the electron dense bodies in the cuticular plate, as well as over the electron dense region at the junctional complex. There was no label over the electron dense portion of the stereocilia rootlets. Tropomyosin is observed in the area of the stereocilia rootlets by immunofluorescent techniques, but like fimbrin, the antigenic sites of tropomyosin did not withstand processing for ultrastructural localization. Tubulin is not present in the apical region of inner or outer hair cells, although its presence could be documented in the hair cell body and in the supporting cells.
journal_name
Hear Resjournal_title
Hearing researchauthors
Slepecky N,Chamberlain SCdoi
10.1016/0378-5955(85)90029-2subject
Has Abstractpub_date
1985-01-01 00:00:00pages
245-60issue
3eissn
0378-5955issn
1878-5891pii
0378-5955(85)90029-2journal_volume
20pub_type
杂志文章相关文献
HEARING RESEARCH文献大全abstract：:We are studying the interconnections between the anteroventral cochlear nucleus (AVCN) and the dorsal cochlear nucleus (DCN). Biotinylated dextran was injected into the DCN, where the best frequency of responses was also recorded. Ventrotubercular neurons in AVCN were labeled, along with cochlear nerve fibers and the ...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(98)00224-x
更新日期：1999-04-01 00:00:00
abstract：:A series of organic acid transport inhibitors significantly reduced the endocochlear potential (EP) decline produced by furosemide in the chinchilla. Probenecid, sodium salicylate and penicillin G were much more effective than novobiocin, meclofenamate or diatrizoate. Inhibitors of organic base transport, choline and ...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(87)90037-2
更新日期：1987-01-01 00:00:00
abstract：OBJECTIVE:Molecular analysis using archival human inner ear specimens is challenging because of the anatomical complexity, long-term fixation, and decalcification. However, this method may provide great benefit for elucidation of otological diseases. Here, we extracted mRNA for RT-PCR from tissues dissected from archiv...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2013.04.008
更新日期：2013-08-01 00:00:00
abstract：:The known structural and functional features of the inferior colliculus provided an advantageous substrate for examining the outcome of neural transplantation. In the present study, tissue from the midbrain tectum of Sprague-Dawley rat fetuses was removed at 15, 16 and 19 days of gestation (E15, E16 and E19), incubate...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(90)90123-7
更新日期：1990-05-01 00:00:00
abstract：:Light microscopy and immunohistochemical analyses of a freshly prepared human cochlea, removed at meningioma skull base surgery, were performed with particular emphasis on synaptophysin (SY) reactivity. Synaptophysin, a 38-kDa glycoprotein, is one of the most abundant integral membrane proteins of small presynaptic ve...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(03)00228-4
更新日期：2003-11-01 00:00:00
abstract：:It is widely appreciated that the key predictor of the pitch of a sound is its periodicity. Neural structures which support pitch perception must therefore be able to reflect the repetition rate of a sound, but this alone is not sufficient. Since pitch is a psychoacoustic property, a putative cortical code for pitch m...
journal_title：Hearing research
pub_type： 杂志文章,评审
doi：10.1016/j.heares.2010.04.015
更新日期：2011-01-01 00:00:00
abstract：:Previous studies have shown a reduction of the ototoxicity of furosemide in chinchillas pretreated with organic acid transport inhibitors. The current studies were designed to investigate whether such a protective effect could be observed in chinchillas receiving ethacrynic acid. Chinchillas weighing 400-600 g were in...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(90)90142-c
更新日期：1990-06-01 00:00:00
abstract：:Stress can be a significant factor influencing ear pathologies and is often reported to trigger the symptoms of Meniere's disease. Both physiological and psychological stress provokes the release of prolactin from the pituitary thus allowing the classification of prolactin as a major stress hormone. We investigated th...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2004.11.015
更新日期：2005-05-01 00:00:00
abstract：:We performed transient evoked otoacoustic emission (TEOAE) measurements on 29 ears of myasthenia gravis (MG) patients. The purpose of the study was to support the role of acetylcholine (ACh) in the efferent innervation of cochlear outer hair cells (OHCs). Another aim was to establish additional diagnostic tools for th...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(98)00154-3
更新日期：1998-12-01 00:00:00
abstract：:The IgG1 mouse monoclonal antibody (MAb) KHRI-3, binds to an antigen of 65-68 kDa expressed on inner ear supporting cells in guinea pigs. We previously showed [Nair et al. (1995) Monoclonal antibody induced hearing loss. Hear. Res. 83, 101-113] that mice carrying the KHRI-3 hybridoma develop high frequency hearing los...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(97)00024-5
更新日期：1997-05-01 00:00:00
abstract：:The cochlear microphonic (CM) is produced by a change in standing currents during the motion of the cochlear partition. The motion of the partition and associated hair cell transduction processes are nonlinear and are reflected in the variation of the instantaneous frequency (IF) of the CM. Although the CM is dominate...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(02)00642-1
更新日期：2002-12-01 00:00:00
abstract：:Sound-induced displacement responses in the plane of the organ of Corti were studied in the apical turn in the isolated temporal-bone preparation of the guinea-pig cochlea. Swept sinusoidal sound stimuli (100-500 Hz) were delivered closed-field to the external auditory meatus. The surface of the organ of Corti was con...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(95)00002-l
更新日期：1995-03-01 00:00:00
abstract：:The tension fibroblasts of the spiral ligament of the mammalian cochlea are thought to create radial tension on the basilar membrane. Their postnatal development was investigated in the gerbil (Meriones unguiculatus) with confocal fluorescence microscopy using phallotoxin as a specific marker for F-actin. In the adult...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(97)00051-8
更新日期：1997-06-01 00:00:00
abstract：:Membranous vestibular labyrinths from the oyster toadfish, Opsanus tau, were fixed, dissected from the animal, stained, and embedded in rectangular blocks of clear histological resin. Photomicrographs of complete embedded labyrinths were taken from six orthogonal directions and used to construct three-dimensional (3D)...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(98)00108-7
更新日期：1998-10-01 00:00:00
abstract：:Membrane-bound guanylyl cyclases (GCs) are peptide hormone receptors whereas the cytosolic isoforms are receptors for nitric oxide. In the inner ear, the membrane-bound GCs may be involved in the regulation of fluid homeostasis and the cytosolic forms possibly play a role in signal processing and regulation of local b...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(98)00176-2
更新日期：1999-01-01 00:00:00
abstract：:We report the changes in auditory function that occurred at selected intervals following exposure to an intense pure-tone stimulus. One day old chicks were exposed to a 0.9 kHz tone for 48 h at 120 dB. At 0, 1, 3, 6, 9, 12 and 15 days after exposure, cochlear nucleus sound-evoked potentials were used to assess thresho...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(89)90012-9
更新日期：1989-09-01 00:00:00
abstract：:Normal-hearing listeners can perceptually segregate concurrent sound sources, but listeners with significant hearing loss or who wear a cochlear implant (CI) lag behind in this ability. Perceptual grouping mechanisms are essential to segregate concurrent sound sources and affect comodulation masking release (CMR). Thu...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2012.11.023
更新日期：2013-02-01 00:00:00
abstract：:Noise trauma, aging, and ototoxicity preferentially damage the outer hair cells of the inner ear, leading to increased hearing thresholds and poorer frequency resolution. Whereas outer hair cells make synaptic connections with less than 10% of afferent auditory nerve fibers (type-II), inner hair cells make connections...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2013.03.012
更新日期：2013-08-01 00:00:00
abstract：:Songbirds spend much of their time learning, producing, and listening to complex vocal sequences we call songs. Songs are learned via cultural transmission, and singing, usually by males, has a strong impact on the behavioral state of the listeners, often promoting affiliation, pair bonding, or aggression. What is it ...
journal_title：Hearing research
pub_type： 杂志文章,评审
doi：10.1016/j.heares.2013.08.016
更新日期：2014-02-01 00:00:00
abstract：:Six utricular hair bundles from a red-eared turtle are modeled using 3-D finite element analysis. The mechanical model includes shear deformable stereocilia, realignment of all forces during force load increments, and tip and lateral link inter-stereocilia connections. Results show that there are two distinct bundle t...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2004.06.006
更新日期：2004-11-01 00:00:00
abstract：:Severe damage to the organ of Corti leads to degeneration of the spiral ganglion cells (SGCs) which form the auditory nerve. This degeneration starts at the level of synaptic connection of the peripheral processes (PPs) of SGCs with the cochlear hair cells. It is generally thought that from this point SGC degeneration...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2020.107928
更新日期：2020-05-01 00:00:00
abstract：:Certain characteristics of the extracellular cochlear microphonic (CM) recorded by intracochlear electrodes change in a bimodal manner as a function of prior acoustic exposure, intensity of stimulation, or stimulus frequency. In the present study, it is shown that biasing the basilar membrane position toward scala tym...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(80)90036-2
更新日期：1980-03-01 00:00:00
abstract：:The human auditory system is comprised of specialized but interacting anatomic and functional pathways encoding object, spatial, and temporal information. We review how learning-induced plasticity manifests along these pathways and to what extent there are common mechanisms subserving such plasticity. A first series o...
journal_title：Hearing research
pub_type： 杂志文章,评审
doi：10.1016/j.heares.2010.03.086
更新日期：2011-01-01 00:00:00
abstract：:Laser scanning confocal microscopy was used to determine the distribution of actin, spectrin and tubulin in whole mounts of the organ of Corti of guinea pig, monkey, rat and chinchilla. Actin, spectrin and tubulin were localized in all cell types in the auditory epithelium. No specialized cytoskeletal organization of ...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(94)90098-1
更新日期：1994-06-01 00:00:00
abstract：:In order to determine the place-frequency map of the rat cochlea, iontophoretic HRP-injections were made into the cochlear nucleus at electrophysiologically characterized positions. Distribution of retrograde HRP transport in cochlear spiral ganglion cells was analysed by means of a three dimensional reconstruction of...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(91)90041-7
更新日期：1991-02-01 00:00:00
abstract：:Potassium channels play a critical role in defining the electrophysiological properties accounting for the unique response patterns of auditory neurons. Serial analysis of gene expression (SAGE), microarrays, RT-PCR, and real-time RT-PCR were used to generate a broad profile of potassium channel expression in the rat ...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2007.01.024
更新日期：2007-06-01 00:00:00
abstract：:We investigated the effects of intracochlear electrical stimulation (ICES) on auditory pathways of neonatal rat deafened by daily amikacin injections. Expression of mRNAs encoding ionotropic glutamate receptor subunits such as alpha-amino-3-hydroxy-5-methyl-4-isoxazole (AMPA) and N-methyl-D-aspartate (NMDA), and gamma...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(00)00167-2
更新日期：2000-12-01 00:00:00
abstract：BACKGROUND:The heterogeneity of tinnitus is substantial. Its numerous pathophysiological mechanisms and clinical manifestations have hampered fundamental and treatment research significantly. A decade ago, the Tinnitus Research Initiative introduced the Tinnitus Sample Case History Questionnaire, a case history instrum...
journal_title：Hearing research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.heares.2019.02.017
更新日期：2019-06-01 00:00:00
abstract：:Previous investigations have shown that a subset of inferior colliculus neurons, which have been designated type O units, respond selectively to isolated features of the cat's head-related transfer functions (HRTFs: the directional transformation of a free-field sound as it propagates from the head to the eardrum). Ba...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2008.01.008
更新日期：2008-04-01 00:00:00
abstract：:In Menière's disease, the increase of extracellular potassium concentration in the perilymph is thought to play a key role in determining the progressive loss of cochlear hair cells. In this paper, we describe a serum-free culture preparation of hair cells from 5 day-old rat and report the release by the cochlea, in r...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(90)90168-o
更新日期：1990-08-01 00:00:00
