Abstract:
OBJECTIVE:Molecular analysis using archival human inner ear specimens is challenging because of the anatomical complexity, long-term fixation, and decalcification. However, this method may provide great benefit for elucidation of otological diseases. Here, we extracted mRNA for RT-PCR from tissues dissected from archival FFPE human inner ears by laser microdissection. METHODS:Three human temporal bones obtained at autopsy were fixed in formalin, decalcified by EDTA, and embedded in paraffin. The samples were isolated into spiral ligaments, outer hair cells, spiral ganglion cells, and stria vascularis by laser microdissection. RNA was extracted and heat-treated in 10 mM citrate buffer to remove the formalin-derived modification. To identify the sites where COCH and SLC26A5 mRNA were expressed, semi-nested RT-PCR was performed. We also examined how long COCH mRNA could be amplified by semi-nested RT-PCR in archival temporal bone. RESULTS:COCH was expressed in the spiral ligament and stria vascularis. However, SLC26A5 was expressed only in outer hair cells. The maximum base length of COCH mRNA amplified by RT-PCR was 98 bp in 1 case and 123 bp in 2 cases. CONCLUSION:We detected COCH and SLC26A5 mRNA in specific structures and cells of the inner ear from archival human temporal bone. Our innovative method using laser microdissection and semi-nested RT-PCR should advance future RNA study of human inner ear diseases.
journal_name
Hear Resjournal_title
Hearing researchauthors
Kimura Y,Kubo S,Koda H,Shigemoto K,Sawabe M,Kitamura Kdoi
10.1016/j.heares.2013.04.008subject
Has Abstractpub_date
2013-08-01 00:00:00pages
26-31eissn
0378-5955issn
1878-5891pii
S0378-5955(13)00101-9journal_volume
302pub_type
杂志文章相关文献
HEARING RESEARCH文献大全abstract::We investigated the effects of intracochlear electrical stimulation (ICES) on auditory pathways of neonatal rat deafened by daily amikacin injections. Expression of mRNAs encoding ionotropic glutamate receptor subunits such as alpha-amino-3-hydroxy-5-methyl-4-isoxazole (AMPA) and N-methyl-D-aspartate (NMDA), and gamma...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(00)00167-2
更新日期:2000-12-01 00:00:00
abstract::In our companion paper (Le Calvez et al., 1998), the levels of distortion product otoacoustic emissions (DPOAE) were collected in the ears of CD1 mice with progressive degeneration of cochlear outer hair cells (OHC). Their comparison to standard functional measurements such as auditory-evoked brainstem responses (ABR)...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(98)00051-3
更新日期:1998-06-01 00:00:00
abstract::Noninvasive auditory brainstem responses (ABRs) are commonly used to assess cochlear pathology in both clinical and research environments. In the current study, we evaluated the relationship between ABR characteristics and more direct measures of cochlear function. We recorded ABRs and auditory nerve (AN) single-unit ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2011.06.002
更新日期:2011-10-01 00:00:00
abstract::Eye color may be an indicator of inner ear melanin content and has been associated with hearing loss. There is controversy as to whether eye color has an effect on acquired causes of sensorineural hearing loss. This review was conducted to analyze the literature evaluating the relationship between eye color and causes...
journal_title:Hearing research
pub_type: 杂志文章,评审
doi:10.1016/j.heares.2014.12.002
更新日期:2015-02-01 00:00:00
abstract::This paper discusses how ion transport proteins in the hair cells of the mammalian cochlea work to produce a sensitive but stable hearing organ. The transport proteins in the inner and outer hair cells are summarized (including their current voltage characteristics), and the roles of these proteins in determining intr...
journal_title:Hearing research
pub_type: 杂志文章,评审
doi:10.1016/j.heares.2011.04.006
更新日期:2011-10-01 00:00:00
abstract::Interaural time and intensity differences (ITD and IID) are important cues in binaural hearing and allow for sound localization, improving speech understanding in noise and reverberation, and integrating sound sources in the auditory scene. Whereas previous research showed that the upper-frequency limit for ITD detect...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2018.09.001
更新日期:2018-12-01 00:00:00
abstract::Single-unit recordings from neurons in the torus semicircularis of Rana ridibunda were analyzed to determine the degree to which these neurons can detect acoustic stimuli superimposed on continuous, broad-band noise. In the first series of trials the stimuli were tone pulses 25 ms long, 5 dB above threshold, and at th...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(88)90117-7
更新日期:1988-09-15 00:00:00
abstract::Adenosine plays a major cytoprotective role during ischemia and conditions of oxidative stress. Previous studies in our laboratory indicate that oxidative stress induces expression of the A1 adenosine receptor (A1AR) via activation of nuclear factor (NF)-kappaB. In this study, we tested whether noise exposure could in...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/S0378-5955(03)00344-7
更新日期:2004-02-01 00:00:00
abstract::The otoprotective peptide AM-111, a cell-permeable inhibitor of JNK mediated apoptosis, was tested for its efficacy as a rescue agent following impulse noise trauma. Single dose administrations of AM-111 at 1h or 4h post-impulse noise exposure (155 dB peak SPL) via systemic or local routes were evaluated with a total ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2006.05.006
更新日期:2007-04-01 00:00:00
abstract::We have studied the noradrenergic and serotonergic changes induced by white noise stimulation at 70, 90 or 110 dB SPL for 45 min, in cochlear nuclei, inferior colliculus (IC), primary auditory cortex (PAC) and as a comparison in locus coeruleus (LC) and raphe dorsalis using HPLC. Both noradrenergic and serotonergic pa...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(98)00031-8
更新日期:1998-04-01 00:00:00
abstract::Long-term experience enhances neural representation of temporal attributes of pitch in the brainstem and auditory cortex in favorable listening conditions. Herein we examine whether cortical pitch mechanisms shaped by language experience are more resilient to degradation in background noise, and exhibit greater binaur...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2017.09.006
更新日期:2017-11-01 00:00:00
abstract::The suppression tuning properties of the oto-acoustic distortion product emission, 2f1-f2 have been measured in the ear canal of gerbil and man. The results show the acoustic response to be suppressible in a similar, frequency-dependent manner in both species. Frequencies near to those of the stimulating tones are mos...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(84)90092-3
更新日期:1984-01-01 00:00:00
abstract::The effects of endogenous and exogenous Ca(2+) buffers on Ca(2+) current kinetics have been investigated by patch clamp in hair cells mechanically isolated from frog semicircular canals. This preparation displays at least three different Ca(2+) channel types: transient currents flow through a drug-resistant channel ("...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2004.05.009
更新日期:2004-09-01 00:00:00
abstract::Sharp spatial selectivity is critical to auditory performance, particularly in pitch-related tasks. Most contemporary cochlear implants have employed monopolar stimulation that produces broad electric fields, which presumably contribute to poor pitch and pitch-related performance by implant users. Bipolar or tripolar ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2011.11.005
更新日期:2012-01-01 00:00:00
abstract::Auditory brainstem responses (ABRs) were evoked in developing wallabies by click and tone burst stimuli delivered by bone conduction and air conduction, at progressive stages of post-natal (pouch) life. ABRs were recorded through the onset of auditory responses (95-110 days), the opening of the external ear canal (125...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(97)00211-6
更新日期:1998-03-01 00:00:00
abstract::Cochleotopic representation in cortex (AI) is extensively reorganized in cats having neonatal, bilateral high frequency cochlear hearing loss. Anterior areas of AI, normally devoted to high frequencies, contain neurons which are almost all tuned to one lower frequency. This frequency corresponds, at the level of the c...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(91)90131-r
更新日期:1991-07-01 00:00:00
abstract::The phase angle, alpha, between a tonal signal and a tonal masker was varied from 0 degrees to 135 degrees in simultaneous masking, forward masking, and pulsation-threshold paradigms. In all conditions the frequency of the signal and masker was 500 Hz. For forward masking both diotic (MOSO) and dichotic (MOS pi) liste...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(82)90039-9
更新日期:1982-08-01 00:00:00
abstract::Recent evidence suggests that the monkey's short-term memory in audition depends on a passively retained sensory trace as opposed to a trace reactivated from long-term memory for use in working memory. Reliance on a passive sensory trace could render memory particularly susceptible to confusion between sounds that are...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2013.01.011
更新日期:2013-04-01 00:00:00
abstract::Binaural temporal resolution was measured using the discrimination of brief interaural time delays (ITDs). In experiment 1, three listeners performed a 2I-2AFC, ITD-discrimination procedure. ITD changes of 8 to 1024micros were applied to brief probe noises. These probes, with durations of 16 to 362ms, were placed symm...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2008.12.001
更新日期:2009-02-01 00:00:00
abstract::This chapter reviews evidence for functional connections of the somatosensory and auditory systems at the very lowest levels of the nervous system. Neural inputs from the dosal root and trigeminal ganglia, as well as their brain stem nuclei, cuneate, gracillis and trigeminal, terminate in the cochlear nuclei. Terminat...
journal_title:Hearing research
pub_type: 杂志文章,评审
doi:10.1016/j.heares.2011.05.001
更新日期:2011-11-01 00:00:00
abstract::Cochlear implant (CI) users typically have excellent speech recognition in quiet but struggle with understanding speech in noise. It is thought that broad current spread from stimulating electrodes causes adjacent electrodes to activate overlapping populations of neurons which results in interactions across adjacent c...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2013.02.004
更新日期:2013-05-01 00:00:00
abstract::Using transgenic fluorescent reporter mice in combination with an established tissue clearing method, we detail heretofore optically opaque regions of the spiral lamina and spiral limbus where the auditory peripheral nervous system is located and provide insight into changes in cochlear vascular density with ageing. W...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2018.11.012
更新日期:2019-01-01 00:00:00
abstract::Immunolabeling of heregulin, a growth factor that enhances cell proliferation in damaged utricles, and one of its binding receptors, ErbB-2, has been briefly described in the P3 rat cochlea and utricle [Zheng et al. (1999) J. Neurocytol. 28, 901-912]. However, little is known about the distribution of heregulin and it...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(02)00339-8
更新日期:2002-07-01 00:00:00
abstract::Can the vestibular periphery of warm-blooded vertebrates recover functionally from severe sensory hair cell loss? Recent findings in birds suggest a mechanism for recovery but in fact no direct functional evidence has been reported. We produced vestibular hair cell lesions using the ototoxic agent streptomycin sulfate...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(92)90184-o
更新日期:1992-10-01 00:00:00
abstract::Voltage-dependent K+ channels in the basolateral membrane of hair cells in guinea-pig cochlea might contribute to the non-linear current-voltage relationships in these hair cells and, thereby, to generation of the extracellular summating potential (SP). To evaluate the role of K+ channels in the generation of the SP t...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(95)00095-l
更新日期:1995-08-01 00:00:00
abstract::Validity of the neurophysiological model of tinnitus (Jastreboff, 1990), outlined in this paper, was tested on data from multicenter trial of tinnitus masking (Hazell et al., 1985). Minimal masking level, intensity match of tinnitus, and the threshold of hearing have been evaluated on a total of 382 patients before an...
journal_title:Hearing research
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/0378-5955(94)90113-9
更新日期:1994-11-01 00:00:00
abstract::We have estimated the transfer curve relating instantaneous sound pressure in the ear canal to instantaneous receptor current through the outer hair cells (OHCs) in the basal turn of the guinea pig cochlea using the cochlear microphonic (CM) elicited by continuous 200 Hz tones. The transfer curve is well approximated ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(98)00125-7
更新日期:1998-11-01 00:00:00
abstract::A number of studies have shown that the ear can be protected from sound over-exposure, either by activating the cochlear efferent system, or by sound 'conditioning' in which the role of the efferent system is less certain. To study more definitively the molecular basis of deliberately induced cochlear protection from ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(01)00265-9
更新日期:2001-06-01 00:00:00
abstract::In developing neural prostheses, particular success has been realized with cochlear implants. These devices bypass damaged hair cells in the auditory system and electrically stimulate the auditory nerve directly. In contemporary cochlear implants, however, the injected electric current spreads widely along the scala t...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2008.01.011
更新日期:2008-08-01 00:00:00
abstract::Spiral ganglion cell (SGC) degeneration following hair cell loss can be prevented by administration of exogenous neurotrophic factors. Many of these neurotrophic factors, in particular the neurotrophins brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), have been described to be involved in the develo...
journal_title:Hearing research
pub_type: 杂志文章,评审
doi:10.1016/j.heares.2012.03.002
更新日期:2012-06-01 00:00:00