Abstract:
:Membrane-bound guanylyl cyclases (GCs) are peptide hormone receptors whereas the cytosolic isoforms are receptors for nitric oxide. In the inner ear, the membrane-bound GCs may be involved in the regulation of fluid homeostasis and the cytosolic forms possibly play a role in signal processing and regulation of local blood flow. In this comprehensive study, we examined, qualitatively and quantitatively, the transcription pattern of all known GC isoforms in the inner ear from rat by RT-PCR. The tissues used were endolymphatic sac, stria vascularis, organ of Corti, organ of Corti outer hair cells, cochlear nerve, Reissner's membrane, vestibular dark cells, and vestibular sensory cells. We show that multiple particulate (GC-A, GC-B, GC-D, GC-E, GC-F and GC-G) and several subunits of the heterodimeric cytosolic GCs (alpha1, alpha2, beta1 and beta2) are expressed, albeit at highly different levels. GC-C was not found. GC-A and the soluble subunits alpha1 and beta1 were transcribed ubiquitously. GC-B was present in all tissues except stria vascularis, which contained GC-A and traces of GC-E and GC-G. GC-B was by far the predominant membrane-bound isoform in the organ of Corti (86%), Reissner's membrane (75%) and the vestibulum (80%). Surprisingly, GC-E, a retinal isoform, was detected in significant amounts in the cochlear nerve (8%) and in the organ of Corti (4%). Although the cytosolic GC is a heterodimer composed of an alpha and a beta subunit, the mRNA transcription of these subunits was not stoichiometric. Particularly in the vestibulum, the transcription of the beta1 subunits was at least four-fold higher than of the alpha1 subunit. The data are compatible with earlier suggestions that membrane receptor GCs may be involved in the control of inner ear electrolyte and fluid composition whereas NO-stimulated GC isoforms mainly participate in the regulation of blood flow and supporting cell physiology.
journal_name
Hear Resjournal_title
Hearing researchauthors
Seebacher T,Beitz E,Kumagami H,Wild K,Ruppersberg JP,Schultz JEdoi
10.1016/s0378-5955(98)00176-2subject
Has Abstractpub_date
1999-01-01 00:00:00pages
95-102issue
1-2eissn
0378-5955issn
1878-5891pii
S0378-5955(98)00176-2journal_volume
127pub_type
杂志文章相关文献
HEARING RESEARCH文献大全abstract::Insight into cochlear mechanics can be obtained from semi-analytical and asymptotic solution methods of which the Liouville -Green (LG) method - in another context known as the WKB method - is the most important one. This paper describes the dispersion properties of fluid waves in general terms and develops the LG for...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(84)90101-1
更新日期:1984-02-01 00:00:00
abstract::The US Navy, through an Office of Naval Research (ONR) lead effort on Noise Induced Hearing Loss (NIHL), is investigating methods and techniques to mitigate hearing loss for the crews and warfighters. Hearing protection is a viable and increasingly popular method of reducing hearing exposure for many ship crew members...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2017.01.004
更新日期:2017-06-01 00:00:00
abstract::It is known that adrenocorticotrophic hormone (ACTH)-derived peptides, the so-called melanocortins, can reduce cisplatin-induced neurotoxicity. Recently, our group has found that cisplatin-induced ototoxicity can also be reduced or prevented by treatment with the synthetic melanocortin-like peptide, ORG 2766 (Hamers e...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(98)00194-4
更新日期:1999-02-01 00:00:00
abstract::Using single-unit electrophysiological methods we have mapped sound frequency (or cochleotopic) representation in the auditory cortex of the chinchilla. We describe the surgical approach to expose this area. We report on maps from six subjects and note a considerable variation in shape between individuals. In general,...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(96)00120-7
更新日期:1996-10-01 00:00:00
abstract::The effect of interaural time differences (ITDs) on stream segregation for successive tone bursts was investigated. Obligatory stream segregation was inferred from the threshold for detecting a rhythmic irregularity in an otherwise isochronous sequence of interleaved "A" and "B" tones (task 1). Subjective stream segre...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2012.06.006
更新日期:2012-09-01 00:00:00
abstract::The Federal Food and Drug Administration, or FDA is generally considered a powerful gatekeeper, able to deliver or withhold life-saving cures and create or destroy economic windfalls. As the decades go by, and technologies, diseases, public health demands, and politics evolve, we can identify patterns of change, actio...
journal_title:Hearing research
pub_type: 杂志文章,评审
doi:10.1016/j.heares.2016.11.006
更新日期:2017-06-01 00:00:00
abstract::We confirm the report of Mountain (Mountain, D.C. (1980): Science 210, 77-72) that stimulating the crossed olivocochlear bundle (COCB) can change the magnitude of the distortion product (f2-f1) in the ear-canal sound pressure. Our results are extended to include (2f1-f2) as well as (f2-f1) from anesthetized chinchilla...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(82)90052-1
更新日期:1982-02-01 00:00:00
abstract:OBJECTIVE:Molecular analysis using archival human inner ear specimens is challenging because of the anatomical complexity, long-term fixation, and decalcification. However, this method may provide great benefit for elucidation of otological diseases. Here, we extracted mRNA for RT-PCR from tissues dissected from archiv...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2013.04.008
更新日期:2013-08-01 00:00:00
abstract::Most investigators place reflective beads on the basilar membrane to measure its vibration with optical methods. It is therefore important to find out if the beads faithfully follow the motion of the structures on which they are placed. Vibration of the beads on the basilar membrane and basilar membrane adjacent to th...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(97)00200-1
更新日期:1998-02-01 00:00:00
abstract::Electrically evoked compound action potentials (ECAPs) have been used to examine temporal response patterns of the auditory nerve in cochlear implant (CI) recipients. ECAP responses to individual pulses in a pulse train vary across stimulation rates for individual CI users. For very slow rates, auditory neurons have a...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2017.06.004
更新日期:2017-08-01 00:00:00
abstract::The acoustic input-admittance at the alligator lizard's tympanic membrane varies with stimulus level; the magnitude of the variation can be as much as a factor of three. At 1.6 kHz, the frequency of maximum admittance magnitude, the admittance varies when the stimulus level exceeds 65 dB SPL. At frequencies above or b...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(84)90110-2
更新日期:1984-12-01 00:00:00
abstract::Cochlear Implant (CI) sound coding strategies based on simultaneous stimulation lead to an increased loudness percept when compared to sequential stimulation using the same current levels. This is due to loudness summation as a result of channel interactions. Studying the loudness perception evoked by dual-channels co...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2020.108091
更新日期:2020-12-01 00:00:00
abstract::Until recently the responses of the mechanosensitive hair cells of the cochlea have been inferred from their morphology, morphological relationships with other structures in the cochlea, and by indirect electrophysiological measurements. With the advent of techniques for making intracellular recordings from hair cells...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(86)90096-1
更新日期:1986-01-01 00:00:00
abstract::Sustained vocalizations of vowels [a], [i], and syllable [mə] were collected in twenty normal-hearing individuals. On vocalizations, five conditions of different audio-vocal feedback were introduced separately to the speakers including no masking, wearing supra-aural headphones only, speech-noise masking, high-pass no...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2015.02.005
更新日期:2015-06-01 00:00:00
abstract::Compound action potentials (AP) were recorded under various stimulus conditions in 31 guinea pigs. Stimulus attenuation, decrease of inter-stimulus interval, increase of the level of a continuous wide-band noise maker, and lowering the animal's temperature all resulted in a drop of the AP amplitude and an increase in ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(81)90034-4
更新日期:1981-03-01 00:00:00
abstract::This study investigated the relation between the intelligibility of conversational and clear speech produced by older and younger adults and (a) the acoustic profile of their speech (b) communication effectiveness. Speech samples from 30 talkers from the elderLUCID corpus were used: 10 young adults (YA), 10 older adul...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2018.06.009
更新日期:2018-11-01 00:00:00
abstract::The f2-f1 distortion product otoacoustic emission (DPOAE) can be observed to undergo gradual alterations in amplitude during continuous ipsilateral stimulation with primary tones. In the present experiments, we characterized the dependence of these amplitude alterations on several stimulus variables (intensity, durati...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(95)00041-2
更新日期:1995-05-01 00:00:00
abstract::Normal-hearing listeners can perceptually segregate concurrent sound sources, but listeners with significant hearing loss or who wear a cochlear implant (CI) lag behind in this ability. Perceptual grouping mechanisms are essential to segregate concurrent sound sources and affect comodulation masking release (CMR). Thu...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2012.11.023
更新日期:2013-02-01 00:00:00
abstract::This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2009.12.012
更新日期:2010-01-30 00:00:00
abstract::The role of the endolymphatic sac (ES) in endolymph volume homeostasis is speculative. The present study investigates changes of the ES's epithelia and luminal filling after induction of an acute endolymphatic hydrops. After microinjection of 1.1 mul artificial endolymph into scala media of the cochlea, guinea pigs we...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2004.10.010
更新日期:2005-04-01 00:00:00
abstract::The effects of eliminating the olivocochlear bundle (OCB) on cochlear electromechanical properties were examined by measuring cochlear microphonics (CM) and distortion product otoacoustic emissions (DPOAEs) in chronically de-efferented chinchillas. The OCB fibers to the right ears were successfully sectioned in six ou...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(99)00217-8
更新日期:2000-05-01 00:00:00
abstract::Harmonic complex tones are a particularly important class of sounds found in both speech and music. Although these sounds contain multiple frequency components, they are usually perceived as a coherent whole, with a pitch corresponding to the fundamental frequency (F0). However, when two or more harmonic sounds occur ...
journal_title:Hearing research
pub_type: 杂志文章,评审
doi:10.1016/j.heares.2009.09.012
更新日期:2010-07-01 00:00:00
abstract::Although pulmonary infections caused by Pseudomonas aeruginosa can hardly be eradicated in patients with cystic fibrosis (CF, the most common genetic disease among Caucasians), these patients are mainly treated with intravenous and nebulized tobramycin. Long-term treatment with tobramycin, however, may induce ototoxic...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(97)00191-3
更新日期:1998-01-01 00:00:00
abstract::The primary goal of this study was to characterize the variability in auditory-nerve temporal response patterns obtained with the electrically evoked compound action potential (ECAP) within and across a relatively large group of cochlear-implant recipients. ECAPs were recorded in response to each of 21 pulses in a pul...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2012.01.010
更新日期:2012-03-01 00:00:00
abstract::Three budgerigars and three zebra finches were tested for their ability to detect sinusoidal stimuli in the presence of broadband noise. Masked thresholds for 1, 2, and 4 KHz pure tones were measured with a fixed frequency condition, in which only one test frequency was presented in a session, and with an uncertain fr...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(90)90043-o
更新日期:1990-12-01 00:00:00
abstract::The mastoid air cell system has traditionally been considered to have a passive role in gas exchange and pressure regulation of the middle ear possibly with some acoustic function. However, more evidence has focused on the mucosa of the mastoid, which may play a more active role in regulation of middle ear pressure. I...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2013.03.002
更新日期:2013-07-01 00:00:00
abstract::Older adults, even with clinically normal hearing sensitivity, have auditory perceptual deficits relative to their younger counterparts. This difficulty may in part, be related to a decline in the neural representation of frequency. The purpose of this study was to examine the effect of age on behavioral and physiolog...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2009.11.010
更新日期:2010-06-01 00:00:00
abstract::To investigate the audio-vocal feedback responses of (F0) to hearing amplification in severe-to-profound prelingual hearing loss (SPHL) using power spectral analysis of F0 contour of sustained vowels. Sustained phonations of vowel/a/of seventeen participants with SPHL were acquired with and without hearing-aid amplifi...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2013.04.010
更新日期:2013-08-01 00:00:00
abstract::A model for cochlear mechanics is proposed to take account of its two systems, one passive and one active. The classical passive system stimulates the inner hair cells directly at levels above about 40 dB SL. At intensities below about 60 dB an active process, the 'cochlear amplifier' (CA), somehow provides additional...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(83)90136-3
更新日期:1983-01-01 00:00:00
abstract::Regrowth of peripheral spiral ganglion neuron (SGN) fibers is a primary objective in efforts to improve cochlear implant outcomes and to potentially reinnervate regenerated hair cells. Cyclic adenosine monophosphate (cAMP) regulates neurite growth and guidance via activation of protein kinase A (PKA) and Exchange Prot...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2011.11.010
更新日期:2012-01-01 00:00:00