Abstract：
:The structural characteristics of a human plasma protein analogous to bovine conglutinin were studied. The protein was previously found to bind to complement-reacted IgG in a calcium-dependent and N-acetyl-D-glucosamine-inhibitable manner and it further shows cross-reactivity with anti-bovine conglutinin antibody. By gel permeation chromatography the conglutinin activity in human plasma was localized to fractions containing proteins of Mr at around 700,000. The conglutinin was localized by one ELISA for antigen determinants and by another for biological activity. When analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under non-reducing conditions these fractions were shown to contain proteins of about 300,000. When human conglutinin-like protein, partially purified by affinity chromatography, was analysed unreduced by SDS-PAGE followed by western blotting, the cross-reacting anti-bovine conglutinin antibody bound to a protein with an Mr of 330,000. When the sample was reduced and alkylated before electrophoresis a band of 66,000 was immunostained. The 330,000 and 66,000 proteins were shown to be collagenase sensitive. 125I-iC3b was seen to bind to the 330,000 band when incubated with western blots of partially purified human conglutinin.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Thiel S,Baatrup G,Friis-Christiansen P,Svehag SE,Jensenius JCdoi
10.1111/j.1365-3083.1987.tb02279.xsubject
Has Abstractpub_date
1987-11-01 00:00:00pages
461-8issue
5eissn
0300-9475issn
1365-3083journal_volume
26pub_type
杂志文章abstract：:alpha 2-macroglobulin (alpha 2M) preparations purified from the plasma of healthy persons and gastric carcinoma patients were further analysed by immunochemical techniques. It was found that only alpha 2M from the plasma of gastric carcinoma patients gave a positive reaction with E-receptor sheep antiserum in rocket i...
journal_title：Scandinavian journal of immunology
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journal_title：Scandinavian journal of immunology
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journal_title：Scandinavian journal of immunology
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journal_title：Scandinavian journal of immunology
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pub_type： 杂志文章
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
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更新日期：1987-10-01 00:00:00
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journal_title：Scandinavian journal of immunology
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journal_title：Scandinavian journal of immunology
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更新日期：1993-01-01 00:00:00
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journal_title：Scandinavian journal of immunology
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1982.tb00680.x
更新日期：1981-05-01 00:00:00
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