Abstract：
:Allograft rejection involves T-cell activation, requiring T-cell receptor interactions with major histocompatibility complex (MHC) molecules and costimulatory signals delivered through the B7-CD28 pathway. We evaluated the effect of blocking this pathway on graft rejection and survival, in a rat experimental model of small bowel transplantation. Heterotopic small bowel transplantation was performed between PVG donor rats and DA recipient rats. The recipient animals were treated with CTLA4-Ig or irrelevant immunoglobulin (Ig)G as control and followed for 18, 30 or 90 days. The survival rate and degree of inflammation and accumulation of CD4+ T cells and macrophages were determined in the transplanted bowels. We found that administration of CTLA4-Ig significantly improved the survival rate compared to control rats: after 30 days 73% of the treated rats had survived and at 90 days 5/8 rats were still living, whereas in the control group only 2/8 rats had survived. The grafts showed preserved mucosal structure with only a mild degree of subacute inflammation and the accumulation of CD4+ T cells and macrophages was noticeably reduced in treated animals as compared to control rats. Necrosis was extensive in control rats, whereas CTLA4-Ig treated animals had grafts with at least some preserved villus morphology and no necrotic tissue. Although small bowel transplantation has proven exceptionally difficult, in this study we have shown that CTLA4-Ig treatment may provide a promising strategy to prevent rejection and induce long term tolerance and graft survival.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Kurlberg G,Haglind E,Schön K,Törnqvist H,Lycke Ndoi
10.1046/j.1365-3083.2000.00725.xkeywords:
subject
Has Abstractpub_date
2000-03-01 00:00:00pages
224-30issue
3eissn
0300-9475issn
1365-3083pii
sji725journal_volume
51pub_type
杂志文章abstract：:Sera genetically deficient in either the alpha-gamma or the beta-subunit of complement component C8 virtually lack haemolytic activity. We have studied the formation and the structural organization of the soluble terminal complement complex (TCC) assembled in these sera following activation with cobra venom factor (CV...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.1998.00390.x
更新日期：1998-09-01 00:00:00
abstract：:TNF-alpha production has a central role in the development and progression of Pseudomonas aeruginosa septic shock. We have previously shown that P. aeruginosa slime-glycolipoprotein (slime-GLP) is the most potent stimulant compared to P. aeruginosa lipopolysaccharide (LPS), for TNF-alpha production and NF-kB activatio...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2007.02053.x
更新日期：2008-02-01 00:00:00
abstract：:In the sera of patients with acute bacterial infections specific autoantibodies (sIAPa) of the immunoglobulin class G (IgG) were found which bind to intestinal alkaline phosphatase (IAP) through the Fab portion. This was demonstrated using immunoaffinity (IA) isolation of sIAPa from patients' sera (particularly bacter...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1995.tb03586.x
更新日期：1995-04-01 00:00:00
abstract：:The J-chain content of 3 IgM and 24 IgA preparations was quantitated by an immunochemical technique after reduction with 20mM dithiothreitol. The amounts released ranged from undetectable (less than 0.1 mg) to 7.3 mg per 100 mg of Ig. Most of the J-chain-deficient proteins were monomeric, but four polymeric IgA prepar...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1976.tb00295.x
更新日期：1976-01-01 00:00:00
abstract：:The detection of mRNA in the murine model of tuberculosis for key cytokines involved in protective immunity in the lung tissues revealed a much faster emergence of the interferon (IFN)-gamma response in the intravenous route than in the aerosol route of inoculation. This slower response in the lungs was associated wit...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.1999.00522.x
更新日期：1999-04-01 00:00:00
abstract：:N-Formyl peptides are cleavage products of bacterial and mitochondrial proteins that have pro-inflammatory activities and play an important role in antibacterial host defence. FcgammaRI is a receptor for the Fc portion of immunoglobulin G expressed in monocytes that mediates cytotoxicity and is upregulated by interfer...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2003.01219.x
更新日期：2003-03-01 00:00:00
abstract：:Normal mouse spleen cells were cultured in different conditioned media (CM). Mixed lymphocyte culture supernatant (MLC SN) was shown to promote the proliferation of cytotoxic, Thy-1+, Lyt-1+, Lyt-2+, asialo-GM-1+, weakly adherent cells with numerous vacuoles and lysosome-like cytoplasmic granules. In contrast, the Con...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1983.tb00846.x
更新日期：1983-08-01 00:00:00
abstract：:Thirty-two mycobacterial components were detected by antibodies contained in leprosy patients' sera across the clinical spectrum and rabbit anti-M. leprae hyperimmune sera by western blot analysis of armadillo-derived M. leprae antigen preparations. Sera of borderline tuberculoid patients were found to contain antibod...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1987.tb02303.x
更新日期：1987-12-01 00:00:00
abstract：:A solid-phase radioimmunoassay for detection of circulating C1q-binding immune complexes (IC) or heat-aggregated IgG (delta IgG) is described. Purified human C1q protein was adsorbed to a fixed area of polystyrene tube surface for 1 hr at 22 degrees C, pH 7.3, mu 0.15. Binding of delta IgG or IC to solid-phase C1q at ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1975.tb02677.x
更新日期：1975-01-01 00:00:00
abstract：:γδ T cells are characterized by recognizing conserved endogenous and stress-induced antigens without antigen presentation. It has been show that γδ T cells play an important role in anti-tumour/microbe responses, but their function in autoimmune diseases is yet not clear. Here, we reported the quantity and phenotype o...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2012.02705.x
更新日期：2012-07-01 00:00:00
abstract：:We have investigated the production of interleukin 2 (IL-2) by human T cells after their stimulation by phytohaemagglutinin (PHA). T cells isolated by rosetting with sheep erythrocytes produced high levels of IL-2. Further separation of rosette-forming cells, according to the expression of Fc receptors for IgG, showed...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1981.tb00570.x
更新日期：1981-09-01 00:00:00
abstract：:Peripheral blood monocyte (PBM) subsets play different roles in inflammatory response and tissue remodelling. The aim of this study was to investigate how allergen challenge affects the number of circulating PBMs in Dermatophagoides pteronyssinus (Dp) allergic patients (Dp-APs). Among 34 Dp-APs challenged, in 22 patie...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2012.02685.x
更新日期：2012-05-01 00:00:00
abstract：:To determine whether psoriatic keratinocytes differ from normal keratinocytes in their response to gamma interferon, epidermal cell suspensions from normal and from lesional and uninvolved psoriatic skin were cultured in the presence of gamma interferon and the induction of HLA-DR expression and inhibition of cell gro...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1988.tb01507.x
更新日期：1988-12-01 00:00:00
abstract：:The identification of a herd of goats with tuberculosis let us test a new treatment regimen against latent tuberculosis infection (LTBI). Using large animal experimental models allows a better approach to understanding human tuberculosis according to immunopathological parameters. Based on an initial study showing a c...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2009.02251.x
更新日期：2009-06-01 00:00:00
abstract：:The cellular and humoral natural immune response induced by hepatitis C virus (HCV) is commonly unable to eradicate the virus. HCV is a highly mutable, hepatotropic RNA virus that causes acute and chronic hepatitis, an infection that involves the production of various cytokines. The aim of the study is to analyse the ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2006.001761.x
更新日期：2006-06-01 00:00:00
abstract：:Crude preparations of platelet-activating factors (PAF) derived by methanolic extraction of supernatants from antigen-challenged basophils generally stimulated the secretion of histamine from human peripheral blood leucocytes when co-incubated with these cells in the absence of an additional stimulator. When these cru...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1981.tb00168.x
更新日期：1981-01-01 00:00:00
abstract：:Human mast cell carboxypeptidase (hMC-CP) is a unique product of mast cells. Unlike tryptase and chymase, its potential function and expression in diseased conditions remain largely unknown. To develop an assay for hMC-CP, the recombinant fusion protein of hMC-CP and purified native skin hMC-CP was prepared, and two n...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2006.01789.x
更新日期：2006-11-01 00:00:00
abstract：:With the aim of producing large quantities of mRNA-transfected monocyte-derived dendritic cells (DCs) to be used as cancer vaccines, a new clinical grade procedure has been developed. Peripheral blood mononuclear cells (PBMCs) obtained by leukapheresis were enriched for monocytes by immunomagnetic depletion of CD19+ B...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2003.01333.x
更新日期：2003-11-01 00:00:00
abstract：:Two cytokines, interferon (IFN) and interleukin 2 (IL-2), activate murine natural killer (NK) cells in vitro. Together both factors synergize considerably. Antibody against IFN eliminates the response of NK cells to IFN as well as to IL-2, whereas antibody against IL-2 blocks the effect of IL-2 but not of IFN. These f...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1985.tb01895.x
更新日期：1985-10-01 00:00:00
abstract：:One successful approach to generate dendritic cells (DC) is to cultivate peripheral blood monocytes in fetal calf serum (FCS)-containing medium in the presence of granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin (IL)-4. Because the use of xenogenic proteins has to be strictly avoided for clini...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2000.00690.x
更新日期：2000-04-01 00:00:00
abstract：:Previously reported experiments suggested that an esterase or a protease, or both, might participate in the expression of human leukocyte migration inhibitory factor (LIF). To clarify this further, a wide variety of simple ester were tested for the ability to protect LIF against inactivation by the serine esterase inh...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1977.tb00328.x
更新日期：1977-01-01 00:00:00
abstract：:Various surface molecules are expressed by activated T cells. Among them, the CD30 antigen has been proposed as a reproducible marker that identifies a subset of differentiated and/or activated T lymphocytes that produce T helper (Th)-2-type cytokines, i.e. interleukin (IL)-4 and IL-5. However, because CD30 has mainly...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.1997.d01-416.x
更新日期：1997-04-01 00:00:00
abstract：:The fibril in primary amyloidosis (AL) is composed of a monoclonal light chain or portions thereof. No unique primary structure has been identified that predisposes certain light chains to form amyloid fibrils. Currently, classification of amyloidosis is based on the biochemistry of the amyloid fibril. We determined t...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1985.tb01877.x
更新日期：1985-09-01 00:00:00
abstract：:The effect of anti-Igs in combination with the tumour promotor TPA on DNA and Ig synthesis in a human follicular germinal centre cell lymphoma carrying sIgM and sIgD was investigated. The lymphoma cells responded to both anti-mu and anti-delta chains and to anti-lambda light chains by DNA synthesis, as measured by met...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1983.tb00778.x
更新日期：1983-02-01 00:00:00
abstract：:The binding of human serum amyloid P component (SAP) to blood cells and monocyte-derived dendritic cells was investigated by flow cytometry. Monocytes bound biotinylated SAP with avidity in a dose-dependent and saturable manner. By contrast, the binding of SAP to monocyte-derived dendritic cells was weak. No binding t...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2006.01774.x
更新日期：2006-07-01 00:00:00
abstract：:CD8+ T cells are thought to play an important role in protective immunity against tuberculosis. We report the identification of three peptides derived from Rv1818c, Rv3812 and Rv3018c proteins of Mycobacterium tuberculosis that bound to HLA-A*0201 molecules and their ability to induce in vitro T-cell response in perip...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2008.02078.x
更新日期：2008-04-01 00:00:00
abstract：:Immunoglobulin G (IgG) is important in clearance and recognition of previously presented antigens and after activation, IgGs can interact with the Fc gamma receptors (FcγRs) on haematopoietic cells, including bone-resorbing osteoclasts. The pathogenicity of IgG, that is the ability to elicit stimulatory effects via Fc...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/sji.13009
更新日期：2020-12-15 00:00:00
abstract：:Experimental amyloidosis was induced in mice by intraperitoneal injections of endotoxin (lipopolysaccharide (LPS)). In addition to LPS, a group of mice received high-density lipoprotein (HDL)-SAA complexes isolated from human acute-phase serum, whereas a group of control mice received saline in addition to LPS. Isolat...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1985.tb01431.x
更新日期：1985-03-01 00:00:00
abstract：:L929, 3T12-3, B16, 3LL, and YAC1 cells with cytostatic factor (CF)-inducing activity from Lactobacillus casei-elicited murine peritoneal macrophages (LCEPM) were susceptible to the cytostatic activity of LCEPM and to LCEPM-produced CF, but L1210, P388D1, and Colon 26 cells, which have no CF-inducing activity, were res...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1988.tb02346.x
更新日期：1988-03-01 00:00:00
abstract：:The enhancement of the lytic capacity of mouse splenic 'natural killer' (NK) cells by interferon has been studied in vitro as a model for NK cell differentiation from inactive immediate precursors. We show that the increased cytotoxicity is a function of interferon concentration, and that two stages in the NK cell dif...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1980.tb00040.x
更新日期：1980-01-01 00:00:00
