Abstract：
:Myocardial lipoprotein lipase (LPL) activity during ischaemia has not been fully understood, although it plays an important role in regulating myocardial fatty acid metabolism. In this experiment, the effects of ischaemia (Experiment A) and anoxia (Experiment B) on two distinct fractions of LPL, i.e. functional and nonfunctional forms were investigated in isolated, perfused rat heart. In Experiment A, hearts were perfused by Neely-Morgan working heart mode with Krebs Henseleit bicarbonate (KHB) buffer (95% O2:5% CO2), then whole heart ischaemia was induced by using a one-way aortic valve, simultaneously switching to the same buffer but containing heparin (5 mu/ml) for 20 min. In Experiment B, the hearts were perfused by Langendorff method with KHB buffer (95% O2:5% CO2) and the buffer was switched to KHB (95% N2:5% CO2) containing heparin for 20 min. Coronary effluent was collected at 5 min intervals and used for the measurement of functional LPL activities using 3H-glyceryltrioleate. The hearts were quickly frozen at the end of perfusion and homogenized. The suspension was used for the measurement of non-functional LPL activities. In Experiment A, functional LPL activities in ischaemia were significantly lower than in the aerobic condition. On the contrary, the nonfunctional LPL activity in ischaemia was significantly higher than in the aerobic condition. In Experiment B these values were also significantly lower than in the aerobic condition. However, there was no significant difference in non-functional LPL activity between the anoxia and aerobic condition. These results indicate that there is a conversion defect from the precursor of LPL to the functional form of LPL in ischaemia, whereas disturbed LPL synthesis might be involved in anoxic myocardium.
journal_name
Basic Res Cardioljournal_title
Basic research in cardiologyauthors
Mochizuki S,Murase T,Yamaoka H,Ishiki M,Tada N,Nagano Mdoi
10.1007/978-3-662-08390-1_6subject
Has Abstractpub_date
1987-01-01 00:00:00pages
45-52eissn
0300-8428issn
1435-1803journal_volume
82 Suppl 1pub_type
杂志文章abstract：:Long QT syndrome is a potentially life-threatening disease characterized by delayed repolarization of cardiomyocytes, QT interval prolongation in the electrocardiogram, and a high risk for sudden cardiac death caused by ventricular arrhythmia. The genetic type 3 of this syndrome (LQT3) is caused by gain-of-function mu...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-016-0530-0
更新日期：2016-03-01 00:00:00
abstract：:The influence of cardiac cholinergic activation was studied in rats and cats on the induction and maintenance of ventricular fibrillation (VF). Acetylcholine (ACH 2-25 micrograms/kg), in doses which did not cause bradycardia or hypotension, induced appearance of spontaneous VF (duration 2-60 sec.) in 9/20 rats which h...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01908386
更新日期：1984-11-01 00:00:00
abstract：:Hemodynamics and myocardial contractility were evaluated in five unanesthetized calves during acute hypocapnic and isocapnic hypoxia and during acute hypocapnic hypoxia with beta-adrenergic blockade. Both hypocapnic and isocapnic hypoxia, with mean PaO2 levels of 33.1 and 39.1 mm Hg respectively, produced a decline in...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01909762
更新日期：1976-09-01 00:00:00
abstract：:The flow pattern and the paths of fluid particles in a saccular aneurysm located at the bifurcation of an intracranial arterial segment are investigated with a numerical method. A normal physiological flow pattern was assumed as input to the studied segment. The theoretical study is carried out for two different Reyno...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907101
更新日期：1988-01-01 00:00:00
abstract：:Verapamil alleviates symptoms in patients with hypertrophic cardiomyopathy (HCM), but the underlying mechanism of improvement remains speculative. Baseline and dipyridamole myocardial blood flow (MBF) were measured in 15 HCM patients (14 men, 42 +/- 10 years), before and after 4 weeks of verapamil SR 480 mg daily, usi...
journal_title：Basic research in cardiology
pub_type： 临床试验,杂志文章
doi：10.1007/s003950050126
更新日期：1999-02-01 00:00:00
abstract：:A reproducible tourniquet-shock has been produced in hind limbs of dogs by unilateral and bilateral extremity ischemia. The following parameters have been measured for analysing the function of the cardiovascular system: mean aortic pressure, heart rate, cardiac output, intraventricular pressure and left ventricular p...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01927866
更新日期：1976-03-01 00:00:00
abstract：:Transformation of structural, functional, metabolic and molecular characteristics is induced by stimulating fast-twitch muscles with the frequency pattern of a motoneuron normally innervating a slow-twitch muscle. These changes correspond to a transition of a "fast-white" into a "slow-red" muscle. Intermittent stimula...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01906369
更新日期：1977-03-01 00:00:00
abstract：:The purpose of this study was to determine if decreased heart function after hypoxia followed by reoxygenation (H-R) is associated with increased degradation of cardiac myosin light chain 1 (MLC1) by matrix metalloproteinase-2 (MMP-2), and to investigate the effects of the increased level of peroxynitrite in the heart...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-009-0035-1
更新日期：2009-11-01 00:00:00
abstract：:To determine the effect of magnesium on myocardial function and oxidative metabolism after reperfusion, isolated rat hearts perfused retrogradely with erythrocyte-enriched medium (0.4 mM palmitate bound to 0.4 mM albumin, 11 mM glucose) were subjected to 60 minutes of no-flow ischemia followed by 60 minutes of reperfu...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00795204
更新日期：1994-07-01 00:00:00
abstract：BACKGROUND:The phytosterol guggulsterone is a potent anti-inflammatory mediator with less side effects than classic steroids. This study assesses the impact of guggulsterone on tissue factor (TF) expression and thrombus formation. METHODS AND RESULTS:Guggulsterone inhibited TNF-alpha-induced endothelial TF protein exp...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-008-0757-5
更新日期：2009-05-01 00:00:00
abstract：:The purpose of this study was to obtain more information about the role of the plasma membrane in cellular uptake of tracers used in myocardial scintigraphy (radioactive thallium chloride and palmitic acid). This knowledge is of importance for an adequate interpretation of the results obtained under clinical condition...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/978-3-662-11041-6_12
更新日期：1985-01-01 00:00:00
abstract：:The mechanism of ergonovine-provoked coronary vasospasm is poorly understood. We tested the effect of ergonovine in perfused hearts from normal and cholesterol-fed (18 weeks, 2% cholesterol diet) rabbits in a constant-flow Langendorff perfusion. Aortic perfusion pressure was monitored to measure coronary vascular resi...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF02070165
更新日期：1983-07-01 00:00:00
abstract：:Autologous bone marrow mononuclear cell (BM-MNC) therapy for patients with ST-segment elevation myocardial infarction (STEMI) has produced inconsistent results, possibly due to BM-MNC product heterogeneity. Patient-specific cardiovascular risk factors (CRFs) may contribute to variations in BM-MNC composition. We sough...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-016-0592-z
更新日期：2017-01-01 00:00:00
abstract：:Local photodynamic therapy was used to prevent neointimal hyperplasia after stenting. Slotted tube stents were placed with 16 atmospheres in porcine femoral arteries with the external diameter to match the diameter at 10 atmospheres. Animals were randomly allocated to Group 1 (stenting only); or Group 2 with stenting,...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950200003
更新日期：2002-03-01 00:00:00
abstract：:The concept of metabolic coronary flow control provides a rationale for the close relationship of coronary flow and myocardial metabolic rate of oxygen. The concept is based on the presence of an oxygen (metabolic) sensor coupled functionally to effector mechanisms, which control vascular tone. Four modes of metabolic...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s00395-006-0621-4
更新日期：2006-11-01 00:00:00
abstract：:We chronically implanted hygroscopic occluders around the left circumflex coronary artery in 49 anesthetized young male domestic pigs and we studied the development of a collateral circulation at 4, 8, 12, and 26 weeks after implantation. At these time intervals groups of animals were again anesthetized, the hearts we...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01908204
更新日期：1989-09-01 00:00:00
abstract：:Atherosclerotic plaque rupture is the key event in the pathogenesis of acute coronary syndromes and it also occurs during coronary interventions. Atherosclerotic plaque rupture does not always result in complete thrombotic occlusion of the epicardial coronary artery with subsequent impending myocardial infarction, but...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s00395-006-0616-1
更新日期：2006-09-01 00:00:00
abstract：:The aim of the present study was to evaluate the expression of hedgehog (Hh) signaling molecules and the chemotactic activity of Sonic hedgehog (Shh) in monocytes from control (CTR) and diabetic patients with or without coronary artery disease (CAD). Previously several studies demonstrated that exogenous administratio...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-009-0047-x
更新日期：2010-01-01 00:00:00
abstract：:Complete length-tension diagrams of myocardium were measured on isolated papillary muscles of cat right ventricle. These diagrams clearly demonstrate that irrespective of loading conditions there is no uniform maxima curve. As a function of preload the curves of afterloaded isotonic maxima obey totally separate nonlin...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907862
更新日期：1985-11-01 00:00:00
abstract：:Increase of Ca2+ concentration to 7.5 mM in the presence of 10 mM or 20 mM caffeine shifted length-tension relationships of 5 rat trabecular muscles and 5 cat papillary muscles to higher tensions. At 1max diastolic tension was enhanced from (formula: see text), for all measured points of stress-strain relationships af...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01906525
更新日期：1978-09-01 00:00:00
abstract：:Hypertension and resulting left ventricular hypertrophy was induced in young male Wistar rats (60 to 70 days old) by narrowing of one renal artery (Goldblatt II). 8 and 24 weeks after operation, myocardial oxygen consumption was measured on a modified in situ heart-lung preparation with nearly isovolumetric left ventr...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01908041
更新日期：1982-05-01 00:00:00
abstract：:The assessment of endothelium-mediated modulation of coronary vasomotor tone in the intact human circulation under physiologic conditions requires very precise determination of both epicardial artery diameters, reflecting effects within the conduit vessels, as well as coronary blood flow, reflecting effects within the...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/978-3-642-72461-9_18
更新日期：1991-01-01 00:00:00
abstract：:We studied TTX-sensitive Na channels in dissociated single ventricular cells from neonatal rats using the patch clamp method for single channel and whole cell recording. In both cases, slowly inactivating or window currents were observed that decayed in a biexponential fashion. Customary models of Na current kinetics ...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:The hypothesis was tested that vardenafil, a PDE5 inhibitor, specifically enhances coronary vasodilation during acidosis. In isolated constant pressure perfused guinea pig hearts, infusion of vardenafil ( journal_title：Basic research in cardiology pub_type： 杂志文章 doi：10.1007/s00395-006-0630-3 更新日期：2007-03-01 00:00:00
abstract：AIMS:There is a need for better identification of patients at high risk for malignant arrhythmias who would benefit from implantable cardioverter defibrillators (ICD). The purpose of this study was to assess whether the C825T polymorphism in the G-protein beta3 subunit gene, GNB3, might serve as a genetic marker for re...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s00395-006-0600-9
更新日期：2006-09-01 00:00:00
abstract：:Experiments were performed to determine the relative contributions of direct Ca(2+)-entry through the L-type Ca(2+)-current and of Ca(2+)-release from the sarcoplasmic reticulum (s.r.) to the intracellular [Ca2+]i-transient in isolated human atrial and ventricular myocytes from patients with severe heart failure and f...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF00794074
更新日期：1997-01-01 00:00:00
abstract：:In the human ventricle two isoforms of the phosphorylatable myosin light chain (MPLC) are expressed. These two forms are designated with increasing acidity as LC-2 and LC-2*. In the normal human heart the relation between LC-2/LC-2*-expression is 70/30, suggesting the existence of three different myosin isoenzymes (MP...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/978-3-642-72474-9_11
更新日期：1992-01-01 00:00:00
abstract：:Verapamil, at a dose of 1 mg/kg, was given intravenously to anesthetized cats one hour after coronary artery occlusion. Verapamil significantly reduced mean arterial blood pressure, but produced an increase in heart rate, partially offsetting the reduction in myocardial oxygen demand resulting from the reduction in pr...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/BF01907648
更新日期：1979-09-01 00:00:00
abstract：:Light in the near-infrared (NIR) region between 700-900 nm can penetrate deep into living tissue, thereby offering a unique opportunity to use near-infrared fluorescence (NIRF) imaging techniques to detect and visualize fluorescent probes in-vivo. In the past few years, many novel NIR fluorescent probes have been desi...
journal_title：Basic research in cardiology
pub_type： 杂志文章,评审
doi：10.1007/s00395-008-0702-7
更新日期：2008-03-01 00:00:00
abstract：:Nitric oxide (NO) exerts several effects on myocardial contraction, including enhancement of relaxation and diastolic function, modulation of beta-adrenergic inotropic responses, and inotropic effects in the absence of agonist pre-stimulation. Different effects have been observed in different species and preparations,...
journal_title：Basic research in cardiology
pub_type： 杂志文章
doi：10.1007/s003950050096
更新日期：1998-08-01 00:00:00
