Abstract:
:Protein phosphorylation enables a rapid adjustment of cellular activities to diverse intracellular and environmental stimuli. Many phosphoproteins are targeted on more than one site, which allows the integration of multiple signals and the implementation of complex responses. However, the hierarchy and interplay between multiple phospho-sites are often unknown. Here, we study multi-site phosphorylation using the yeast trehalase Nth1 and its activator, the 14-3-3 protein Bmh1, as a model. Nth1 is known to be phosphorylated by the metabolic kinase PKA on four serine residues and by the cell cycle kinase CDK on one residue. However, how these five phospho-sites adjust Nth1 activity remains unclear. Using a novel reporter construct, we investigated the contribution of the individual sites for the regulation of the trehalase and its 14-3-3 interactor. In contrast to the constitutively phosphorylated S20 and S83, the weaker sites S21 and S60 are only phosphorylated by increased PKA activity. For binding Bmh1, S83 functions as the high-affinity "gatekeeper" site, but successful binding of the Bmh1 dimer and thus Nth1 activation requires S60 as a secondary site. Under nutrient-poor conditions with low PKA activity, S60 is not efficiently phosphorylated and the cell cycle dependent phosphorylation of S66 by Cdk1 contributes to Nth1 activity, likely by providing an alternative Bmh1 binding site. Additionally, the PKA sites S20 and S21 modulate the dephosphorylation of Nth1 on downstream Bmh1 sites. In summary, our results expand our molecular understanding of Nth1 regulation and provide a new aspect of the interaction of 14-3-3 proteins with their targets.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Dengler L,Örd M,Schwab LM,Loog M,Ewald JCdoi
10.1038/s41598-020-80357-3subject
Has Abstractpub_date
2021-01-13 00:00:00pages
962issue
1issn
2045-2322pii
10.1038/s41598-020-80357-3journal_volume
11pub_type
杂志文章abstract::In the 70's, Robert May demonstrated that complexity creates instability in generic models of ecological networks having random interaction matrices A. Similar random matrix models have since been applied in many disciplines. Central to assessing stability is the "circular law" since it describes the eigenvalue distri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26486-2
更新日期:2018-05-29 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-24189-2
更新日期:2018-04-12 00:00:00
abstract::In this paper, we present the design, fabrication and optical characterization of computer-generated holograms (CGH) encoding information for light beams carrying orbital angular momentum (OAM). Through the use of a numerical code, based on an iterative Fourier transform algorithm, a phase-only diffractive optical ele...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-18147-7
更新日期:2017-12-21 00:00:00
abstract::Vaginal delivery with obstetrical trauma is a risk factor for pelvic organ prolapse later in life. Loss of fibulin-5 (FBLN5), an elastogenesis-promoting cellular matrix protein, results in prolapse in mice. Here, we evaluated effects of pregnancy, parturition, and obstetrical injury on FBLN5 content, elastic fibers, b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63031-6
更新日期:2020-04-14 00:00:00
abstract::Hfq is a bacterial RNA binding protein that carries out several roles in genetic expression regulation, mainly at the post-transcriptional level. Previous studies have shown its importance in growth and virulence of bacteria. Here, we provide the direct observation of its ability to interact with membranes. This was e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11157-5
更新日期:2017-09-06 00:00:00
abstract::The photoactive Orange Carotenoid Protein (OCP) plays a key role in cyanobacterial photoprotection. In OCP, a single non-covalently bound keto-carotenoid molecule acts as a light intensity sensor, while the protein is responsible for forming molecular contacts with the light-harvesting antenna, the fluorescence of whi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68463-8
更新日期:2020-07-16 00:00:00
abstract::Nesfatin-1, a satiety molecule processed from nucleobindin2 (NUCB2), is implicated in visceral hypersensitivity in rats and colocalized with 5-hydroxytryptamine (5-HT) in the dorsal raphe nucleus (DRN). Maternal separation (MS) in rats contributes to visceral hypersensitivity via elevated expression of 5-HT in the DRN...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27592-x
更新日期:2018-06-19 00:00:00
abstract::Environmental cycles often influence the presence of animals, creating patterns at different temporal scales, which may mean that their effects overlap and/or interact. Interactions between diel and seasonal cycles have been reported to influence fish behaviour but little is known about such interactions in marine top...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-38900-4
更新日期:2019-02-22 00:00:00
abstract::The individual response of patients to propofol results from the influence of genetic factors. However, the state of knowledge in this matter still remains insufficient. The aim of our study was to determine genetic predictors of variable pharmacokinetics and pharmacodynamics of propofol within selected 9 genes coding...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-017-15657-2
更新日期:2017-11-13 00:00:00
abstract::Delivery of proteins has been regarded as the safest and most useful application in therapeutic application of stem cells, because proteins can regulate gene expression transiently without any genomic alteration. However, it is difficult to accurately measure efficiency or quantity of intracellular protein uptake. Her...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04378
更新日期:2014-03-14 00:00:00
abstract::As a nutritional factor, folic acid can prevent cardiac and neural defects during embryo development. Our previous study showed that arsenic impairs embryo development by down-regulating Dvr1/GDF1 expression in zebrafish. Here, we investigated whether folic acid could protect against arsenic-mediated embryo toxicity. ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16093
更新日期:2015-11-05 00:00:00
abstract::On standard electrocardiogram (ECG) PQ interval is known to be moderately heart rate dependent, but no physiologic details of this dependency have been established. At the same time, PQ dynamics is a clear candidate for non-invasive assessment of atrial abnormalities including the risk of atrial fibrillation. We studi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59480-8
更新日期:2020-02-13 00:00:00
abstract::Amine quantification is an important strategy in patient stratification and personalised medicine. This is because amines, including amino acids and methylarginines impact on many homeostatic processes. One important pathway regulated by amine levels is nitric oxide synthase (NOS). NOS is regulated by levels of (i) th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31055-8
更新日期:2018-09-18 00:00:00
abstract::A previous randomized, controlled trial had demonstrated that complete intraureteral stent placement (CIU-SP) was superior to conventional stent placement (C-SP) in terms of improvement of stent-related urinary symptoms. However, it is unclear as to which subdomain symptom and cohort could benefit the most from CIU-SP...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-020-72937-0
更新日期:2020-09-28 00:00:00
abstract::Groundwater aquifers are an essential source of drinking water, and must be protected against contamination. Phosphogypsum stacks originating from the processing of phosphate rock contain small amounts of radionuclides, such as 226Ra. In September 2016, a sinkhole located beneath a phosphogypsum stack collapsed under ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29541-0
更新日期:2018-07-30 00:00:00
abstract::There is widespread transcriptional dysregulation in Huntington's disease (HD) brain, but analysis is inevitably limited by advanced disease and postmortem changes. However, mutant HTT is ubiquitously expressed and acts systemically, meaning blood, which is readily available and contains cells that are dysfunctional i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44849
更新日期:2017-03-21 00:00:00
abstract::Guppy (Poecilia reticulata) is an ideal model for studying environmental estrogens, and its large caudal fin has a high capacity to regenerate. This study analyzed the feasibility of caudal fin for detecting vitellogenin (Vtg), the most commonly used biomarker of environmental estrogens. Firstly, a sandwich ELISA for ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06670-6
更新日期:2017-08-09 00:00:00
abstract::Confident characterization of the microheterogeneity of protein glycosylation through identification of intact glycopeptides remains one of the toughest analytical challenges for glycoproteomics. Recently proposed mass spectrometry (MS)-based methods still have some defects such as lack of the false discovery rate (FD...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25102
更新日期:2016-05-03 00:00:00
abstract::We have developed sensitive detection of cancer biomarker vascular endothelial growth factor (VEGF) using the p-n heterojunction comprised of p-type BiOI nanoflakes (NFs) array and n-type TiO2 nanotubes (NTs) array. Due to the unique arrayed structure and the synergy effect of photoelectrochemistry in the formed p-n j...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04426
更新日期:2014-03-21 00:00:00
abstract::Liver repopulation by transplanted hepatocytes has not been achieved previously in a normal liver microenvironment. Here we report that adult rat hepatocytes transduced ex vivo with a lentivirus expressing a human YapERT2 fusion protein (hYapERT2) under control of the hepatocyte-specific transthyretin (TTR) promoter r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19275
更新日期:2016-01-14 00:00:00
abstract::Bacteriophage capsids constitute icosahedral shells of exceptional stability that protect the viral genome. Many capsids display on their surface decoration proteins whose structure and function remain largely unknown. The decoration protein pb10 of phage T5 binds at the centre of the 120 hexamers formed by the major ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41662
更新日期:2017-02-06 00:00:00
abstract::Hydrogen has therapeutic and preventive effects against various diseases. Although animal and clinical studies have reported promising results, hydrogen distribution in organs after administration remains unclear. Herein, the sequential changes in hydrogen concentration in tissues over time were monitored using a high...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-38180-4
更新日期:2019-02-04 00:00:00
abstract::Glaucoma is a group of progressive optic neuropathies that share common biological and clinical characteristics including irreversible changes to the optic nerve and visual field loss caused by the death of retinal ganglion cells (RGCs). The loss of RGCs manifests as characteristic cupping or optic nerve degeneration,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68811-8
更新日期:2020-07-16 00:00:00
abstract::Daily sunlight exposure damages the epidermal basement membrane (BM) and disrupts epidermal homeostasis. Inter-follicular epidermal stem cells (IFE-SCs) regulate epidermal proliferation and differentiation, which supports epidermal homeostasis. Here, we examine how photoaging affects the function of IFE-SCs and we ide...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69558-y
更新日期:2020-07-28 00:00:00
abstract::Compact and power-efficient plastic electronic synapses are of fundamental importance to overcoming the bottlenecks of developing a neuromorphic chip. Memristor is a strong contender among the various electronic synapses in existence today. However, the speeds of synaptic events are relatively slow in most attempts at...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01619
更新日期:2013-01-01 00:00:00
abstract::The aim of this study was to identify genes for which the expression within carotid atherosclerosis was reproducibly associated with the symptoms of cerebral embolization. Two publically available microarray datasets E-MEXP-2257 and GSE21545 were analysed using GeneSpring 11.5. The two datasets utilized a total of 22 ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04497-9
更新日期:2017-06-28 00:00:00
abstract::Transformation optics (TO) concept well known for its huge possibility in patterning the path of electromagnetic waves is exploited to design a beam steering lens. The broadband directive in-phase emission in a desired off-normal direction from an array of equally fed radiators is numerically and experimentally report...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18819
更新日期:2016-01-05 00:00:00
abstract::Free-living animals must make dietary choices in terms of chemical and physical properties, depending on their digestive physiology and availability of food resources. Here we comprehensively evaluated the dietary choices of proboscis monkeys (Nasalis larvatus) consuming young leaves. We analysed the data for leaf tou...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42774
更新日期:2017-02-17 00:00:00
abstract::To investigate the concentrations of rare earth elements in cereals and assess human health risk through cereal consumption, a total of 327 cereal samples were collected from rare earth mining area and control area in Shandong, China. The contents of 14 rare earth elements were determined by Inductively Coupled Plasma...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-10256-7
更新日期:2017-08-29 00:00:00
abstract::Some studies have demonstrated interactions of AD-risk single nucleotide polymorphisms (SNPs) in non-APOE regions with APOE genotype. Nevertheless, no study reported interactions of expression quantitative trait locus (eQTL) for APOE with APOE genotype. In present study, we included 9286 unrelated AD patients and 8479...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-26398-1
更新日期:2018-05-23 00:00:00