Abstract:
:While microbiome plays key roles in the etiology of multiple sclerosis (MS), its mechanism remains elusive. Here, we conducted a comprehensive metagenome-wide association study (MWAS) of the relapsing-remitting MS gut microbiome (ncase = 26, ncontrol = 77) in the Japanese population, by using whole-genome shotgun sequencing. Our MWAS consisted of three major bioinformatic analytic pipelines (phylogenetic analysis, functional gene analysis, and pathway analysis). Phylogenetic case-control association tests showed discrepancies of eight clades, most of which were related to the immune system (false discovery rate [FDR] < 0.10; e.g., Erysipelatoclostridium_sp. and Gemella morbillorum). Gene association tests found an increased abundance of one putative dehydrogenase gene (Clo1100_2356) and one ABC transporter related gene (Mahau_1952) in the MS metagenome compared with controls (FDR < 0.1). Molecular pathway analysis of the microbiome gene case-control comparisons identified enrichment of multiple Gene Ontology terms, with the most significant enrichment on cell outer membrane (P = 1.5 × 10-7). Interaction between the metagenome and host genome was identified by comparing biological pathway enrichment between the MS MWAS and the MS genome-wide association study (GWAS) results (i.e., MWAS-GWAS interaction). No apparent discrepancies in alpha or beta diversities of metagenome were found between MS cases and controls. Our shotgun sequencing-based MWAS highlights novel characteristics of the MS gut microbiome and its interaction with host genome, which contributes to our understanding of the microbiome's role in MS pathophysiology.
journal_name
Front Cell Infect Microbioljournal_title
Frontiers in cellular and infection microbiologyauthors
Kishikawa T,Ogawa K,Motooka D,Hosokawa A,Kinoshita M,Suzuki K,Yamamoto K,Masuda T,Matsumoto Y,Nii T,Maeda Y,Nakamura S,Inohara H,Mochizuki H,Okuno T,Okada Ydoi
10.3389/fcimb.2020.585973subject
Has Abstractpub_date
2020-12-11 00:00:00pages
585973issn
2235-2988journal_volume
10pub_type
杂志文章abstract::Intracellular bacterial pathogens have evolved distinct lifestyles inside eukaryotic cells. Some pathogens coexist with the infected cell in an obligate intracellular state, whereas others transit between the extracellular and intracellular environment. Adaptation to these intracellular lifestyles is regulated in both...
journal_title:Frontiers in cellular and infection microbiology
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pub_type: 杂志文章
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doi:10.3389/fcimb.2019.00271
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pub_type: 杂志文章
doi:10.3389/fcimb.2019.00472
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doi:10.3389/fcimb.2018.00081
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2013.00021
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2012.00115
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doi:10.3389/fcimb.2020.00265
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pub_type: 杂志文章
doi:10.3389/fcimb.2016.00144
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00169
更新日期:2017-05-05 00:00:00
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00357
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00265
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2016.00038
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00362
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2012.00018
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doi:10.3389/fcimb.2019.00295
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doi:10.3389/fcimb.2017.00201
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2014.00110
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00138
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journal_title:Frontiers in cellular and infection microbiology
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doi:10.3389/fcimb.2020.00276
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.3389/fcimb.2019.00031
更新日期:2019-02-19 00:00:00
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pub_type: 杂志文章
doi:10.3389/fcimb.2016.00008
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2016.00004
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pub_type: 杂志文章
doi:10.3389/fcimb.2019.00244
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pub_type: 杂志文章
doi:10.3389/fcimb.2017.00263
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