Abstract:
:Myocarditis, inflammation of the heart muscle, affects all demographics and is a major cause of sudden and unexpected death in young people. It is most commonly caused by viral infections of the heart, with coxsackievirus B3 (CVB3) being among the most prevalent pathogens. To understand the molecular pathogenesis of CVB3 infection and provide strategies for developing treatments, we examined the role of a key nuclear pore protein 98 (NUP98) in the setting of viral myocarditis. NUP98 was cleaved as early as 2 h post-CVB3 infection. This cleavage was further verified through both the ectopic expression of viral proteases and in vitro using purified recombinant CVB3 proteases (2A and 3C), which demonstrated that CVB3 2A but not 3C is responsible for this cleavage. By immunostaining and confocal imaging, we observed that cleavage resulted in the redistribution of NUP98 to punctate structures in the cytoplasm. Targeted siRNA knockdown of NUP98 during infection further increased viral protein expression and viral titer, and reduced cell viability, suggesting a potential antiviral role of NUP98. Moreover, we discovered that expression levels of neuregulin-1 (NRG1), a cardioprotective gene, and presenilin-1 (PSEN1), a cellular protease processing the tyrosine kinase receptor ERBB4 of NRG1, were reliant upon NUP98 and were downregulated during CVB3 infection. In addition, expression of these NUP98 target genes in myocardium tissue not only occurred at an earlier phase of infection, but also appeared in areas away from the initial inflammatory regions. Collectively, CVB3-induced cleavage of NUP98 and subsequent impairment of the cardioprotective NRG1-ERBB4/PSEN1 signaling cascade may contribute to increased myocardial damage in the context of CVB3-induced myocarditis. To our knowledge, this is the first study to demonstrate the link between NUP98 and the NRG1 signaling pathway in viral myocarditis.
journal_name
Front Cell Infect Microbioljournal_title
Frontiers in cellular and infection microbiologyauthors
Hanson PJ,Hossain AR,Qiu Y,Zhang HM,Zhao G,Li C,Lin V,Sulaimon S,Vlok M,Fung G,Chen VH,Jan E,McManus BM,Granville DJ,Yang Ddoi
10.3389/fcimb.2019.00265subject
Has Abstractpub_date
2019-07-24 00:00:00pages
265issn
2235-2988journal_volume
9pub_type
杂志文章abstract::Shigella is the major cause of bacillary dysentery world-wide. It is divided into four species, named S. flexneri, S. sonnei, S. dysenteriae, and S. boydii, which are distinct genomically and in their ability to cause disease. Shigellosis, the clinical presentation of Shigella infection, is characterized by watery dia...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2017.00064
更新日期:2017-03-24 00:00:00
abstract::Toxoplasma gondii has been found to infect almost all warm-blooded animals, including humans. In this study, a total of 3,275 human serum samples were collected from hospitals in five provinces of China. About 5.13% (168/3,275) (95% CI, 4.42-5.94) of the serum samples tested positive for T. gondii IgG antibody by a mo...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00302
更新日期:2020-06-23 00:00:00
abstract::Intestinal microbes are an important system in the human body, with significant effects on behavior. An increasing body of research indicates that intestinal microbes affect brain function and neurogenesis, including sensitivity to stress. To investigate the effects of microbial colonization on behavior, we examined b...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00489
更新日期:2017-11-30 00:00:00
abstract::Salmonellae are facultative intracellular pathogens that cause globally distributed diseases with massive morbidity and mortality in humans and animals. In the past decades, numerous studies were focused on host defenses against Salmonella infection. Autophagy has been demonstrated to be an important defense mechanism...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2018.00078
更新日期:2018-03-14 00:00:00
abstract::Rheumatoid Arthritis (RA) has been increasingly associated with perturbations to the microbial communities that reside in and on the body (the microbiome), in both human and animal studies. To date, such studies have mainly focused on the microbial communities that inhabit the gut and oral cavity. Mounting evidence su...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00440
更新日期:2020-01-22 00:00:00
abstract::Objectives:Citrobacter freundii is a frequent cause of nosocomial infections and a known cause of diarrheal infections, and has increasingly become multidrug resistant (MDR). In this study, we aimed to determine the genetic diversity, the antimicrobial resistance profiles and in vitro virulence properties of C. freund...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00233
更新日期:2018-07-10 00:00:00
abstract::The Asian tiger mosquito Aedes (Stegomya) albopictus is an invasive species that has spread across the world in the last two decades, showing a great capacity to adapt to contrasting climates and environments. While demonstrated in many insects, the contribution of bacterial symbionts in Aedes ecology is a challenging...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2014.00059
更新日期:2014-05-14 00:00:00
abstract::Toxoplasma gondii is a prevalent parasite of mammals and birds including up to 30% of humans world-wide. Primary infection of immunocompetent hosts leads to a robust cell-mediated immune response, which controls but does not clear the infection, thus enabling long-term parasite persistence in brain and muscle tissues....
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00235
更新日期:2019-06-28 00:00:00
abstract::Herpes simplex virus-1 (HSV-1) is a significant human pathogen. Upon infection, HSV-1 expresses its immediate early (IE) genes, and the IE transcription factor ICP4 (infectious cell protein-4) plays a pivotal role in initiating the downstream gene-expression cascade. Using live-cell time-lapse fluorescence microscopy,...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00265
更新日期:2020-06-24 00:00:00
abstract::In the present study, we determined the in vitro characteristics and binding interactions of chicken PD-1 (chPD-1) and PD-L1 (chPD-L1) and developed a panel of specific monoclonal antibodies against the two proteins. ChPD-1 and chPD-L1 sequence identities and similarities were lower compared with those of humans and o...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00436
更新日期:2019-12-19 00:00:00
abstract::Human coronaviruses, which can cause a range of infectious diseases, have been studied for nearly 60 years. The field has gained renewed interest from researchers around the world due to the COVID-19 outbreak in late 2019. Despite a large amount of research, little is known about the knowledge structure and developing...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.581404
更新日期:2020-09-23 00:00:00
abstract::Colorectal adenocarcinoma (CRC) is a common tumor with high mortality rates. Interestingly, CRC was found to be colonized by the oral anaerobic bacteria Fusobacterium nucleatum, which accelerates tumor progression and enables immune evasion. The CRC-specific colonization by fusobacteria is mediated through the recogni...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00295
更新日期:2017-06-30 00:00:00
abstract::In this original study, we retrospectively reviewed the cases of nocardiosis diagnosed through culture and next-generation sequencing (NGS) methods between 2014 and 2018 in Huashan Hospital and found out that the latter way can not only improve the detection rate of Nocardia spp. but also greatly reduce the turnaround...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00013
更新日期:2020-02-18 00:00:00
abstract::Platycodin D, extract from the root of Platycodon grandiflorum, is one of the most important monomers of the Qinbaiqingfei pellets (Qinbai) that has already been approved as the first Traditional Chinese Medicine for clinic use as an anti-M. pneumoniae agent. Qinbai constituents Scutellaria baicalensis and Platycodon ...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2014.00192
更新日期:2015-01-13 00:00:00
abstract::Pet and EspC are toxins secreted by enteroaggregative (EAEC) and enteropathogenic (EPEC) diarrheagenic Escherichia coli pathotypes, respectively. Both toxins are members of the Serine Protease Autotransporters of Enterobacteriaceae (SPATEs) family. Pet and EspC are important virulence factors that produce cytotoxic an...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00334
更新日期:2019-09-25 00:00:00
abstract::Enteropathogenic Escherichia coli and enterohemorrhagic E. coli are diarrheagenic bacterial human pathogens that cause severe gastroenteritis. These enteric pathotypes, together with the mouse pathogen Citrobacter rodentium, belong to the family of attaching and effacing pathogens that form a distinctive histological ...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2016.00129
更新日期:2016-10-21 00:00:00
abstract::Streptococcus mutans is an oral species closely associated with dental caries. As an early oral colonizer, S. mutans utilizes interspecies coaggregation to promote the colonization of subsequent species and affect polymicrobial pathogenesis. Previous studies have confirmed several adhering partner species of S. mutans...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00344
更新日期:2020-07-07 00:00:00
abstract::Sepsis is a severe syndrome that arises when the host response to an insult is exacerbated, leading to organ failure and frequently to death. How a chronic infection that causes a prolonged Th1 expansion affects the course of sepsis is unknown. In this study, we showed that mice chronically infected with Toxoplasma go...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00116
更新日期:2017-04-07 00:00:00
abstract::Due to the importance of a rapid determination of patients infected by multidrug resistant bacteria, we evaluated two rapid diagnostic tests for the detection of third-generation cephalosporins (3GC)-resistant Enterobacterales directly from positive blood cultures within 1 h: BL-REDTM (electrochemical method) and β-LA...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00491
更新日期:2020-09-11 00:00:00
abstract::Bacteria of the Salmonella genus cause diseases ranging from gastroenteritis to life-threatening typhoid fever and are among the most successful intracellular pathogens known. After the invasion of the eukaryotic cell, Salmonella exhibits contrasting lifestyles with different replication rates and subcellular location...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2017.00432
更新日期:2017-10-04 00:00:00
abstract::Cryptosporidium spp. and Enterocytozoon bieneusi are two important zoonotic pathogens that can infect humans and a broad range of animal hosts. However, few studies have been conducted to study infection of the two pathogens in domestic geese until now. The aims of the present study were to determine the prevalence of...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00416
更新日期:2019-12-04 00:00:00
abstract::MiRNAs are important post-transcriptional regulators of gene expression. MiRNA expression is a crucial part of host responses to bacterial infection, however there is limited knowledge of their impact on the outcome of infections. We investigated the influence of miR-21 on macrophage responses during infection with Li...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00201
更新日期:2017-05-23 00:00:00
abstract::More than a decade ago a short tandem repeat-based typing method was developed for the fungus Aspergillus fumigatus. This STRAf assay is based on the analysis of nine short tandem repeat markers. Interpretation of this STRAf assay is complicated when there are only one or two differences in tandem repeat markers betwe...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00082
更新日期:2019-03-29 00:00:00
abstract::ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanni, Pseudomonas aeruginosa, and Enterobacter species) pathogens have characteristic multiple-drug resistance and cause an increasing number of nosocomial infections worldwide. Peptide-based therapeutics to treat ESKAPE infe...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00041
更新日期:2017-02-15 00:00:00
abstract::The black yeast Exophiala dermatitidis is a widespread polyextremophile and human pathogen, that is found in extreme natural habitats and man-made environments such as dishwashers. It can cause various diseases ranging from phaeohyphomycosis and systemic infections, with fatality rates reaching 40%. While the number o...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2016.00136
更新日期:2016-10-24 00:00:00
abstract::Due to increased travel, climatic, and environmental changes, the incidence of tick-borne disease in both humans and animals is increasing throughout Europe. Therefore, extended surveillance tools are desirable. To accurately screen tick-borne pathogens (TBPs), a large scale epidemiological study was conducted on 7050...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2014.00103
更新日期:2014-07-29 00:00:00
abstract::Dengue fever is an Aedes mosquito-borne illness caused by any one of the four different dengue virus (DENV) serotypes (1-4) and manifests in the form of symptoms ranging from mild or asymptomatic to severe disease with vascular leakage, leading to shock, and viral hemorrhagic syndrome. Increased risk of severe disease...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2020.580096
更新日期:2020-10-02 00:00:00
abstract::Sepsis is a life-threatening health condition caused by infectious pathogens of the respiratory tract, and accounts for 28-50% of annual deaths in the US alone. Current treatment regimen advocates the use of corticosteroids as adjunct treatment with antibiotics, for their broad inhibitory effect on the activity and pr...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00263
更新日期:2017-06-22 00:00:00
abstract::Eradication of Helicobacter pylori has been found to be effective for gastric cancer prevention, but uncertainties remain about the possible adverse consequences such as the potential microbial dysbiosis. In our study, we investigated the association between gut microbiota and H. pylori-related gastric lesions in 47 s...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00202
更新日期:2018-06-19 00:00:00
abstract::Vaccination has had a major impact on the control of infectious diseases. However, there are still many infectious diseases for which the development of an effective vaccine has been elusive. In many cases the failure to devise vaccines is a consequence of the inability of vaccine candidates to evoke appropriate immun...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2013.00013
更新日期:2013-03-25 00:00:00