Molecular Characterization of Circulating Microbiome Signatures in Rheumatoid Arthritis.


:Rheumatoid Arthritis (RA) has been increasingly associated with perturbations to the microbial communities that reside in and on the body (the microbiome), in both human and animal studies. To date, such studies have mainly focused on the microbial communities that inhabit the gut and oral cavity. Mounting evidence suggests that microbial DNA can be detected in the blood circulation using a range of molecular methods. This DNA may represent an untapped pool of biomarkers that have the potential to report on changes to the microbiome of distant sites (e.g., example, the gut and oral cavity). To this end, through amplification and sequencing of the bacterial 16S rRNA variable region four, we evaluated the presence and identity of microbial DNA in blood samples obtained from RA patients (both prior to and 3 months following the instigation of treatment) in comparison to a small number of healthy control subjects and samples obtained from patients with ankylosing spondylitis (AS) and psoriatic arthritis (PA). Bacterial-derived DNA was identified in the majority of our patient samples. Taxonomic classification revealed that the microbiome community in RA was distinct from AS, PA, and the healthy state. Through analysis of paired patient samples obtained prior to and 3 months following treatment (V0 vs. V3), we found the microbiome to be modulated by treatment, and in many cases, this shift reduced the distance between these samples and the healthy control samples, suggesting a partial normalization following treatment in some patients. This effect was especially evident in seronegative arthritis patients. Herein, we provide further evidence for the existence of a blood microbiome in health and identify specific taxa modulated in disease and following treatment. These blood-derived signatures may have significant utility as disease biomarkers and suggest this area warrants further investigation.


Hammad DBM,Hider SL,Liyanapathirana VC,Tonge DP




Has Abstract


2020-01-22 00:00:00








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  • Alterations to the Lung Microbiome in Idiopathic Pulmonary Fibrosis Patients.

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    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章


    authors: Tong X,Su F,Xu X,Xu H,Yang T,Xu Q,Dai H,Huang K,Zou L,Zhang W,Pei S,Xiao F,Li Y,Wang C

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  • Targeting Natural Killer Cells for Improved Immunity and Control of the Adaptive Immune Response.

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    pub_type: 杂志文章,评审


    authors: Pierce S,Geanes ES,Bradley T

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  • 3-Hydroxyphenylacetic acid induces the Burkholderia cenocepacia phenylacetic acid degradation pathway - toward understanding the contribution of aromatic catabolism to pathogenesis.

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    journal_title:Frontiers in cellular and infection microbiology

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  • The Transcriptome of Exophiala dermatitidis during Ex-vivo Skin Model Infection.

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  • Live Attenuated Tularemia Vaccines for Protection Against Respiratory Challenge With Virulent F. tularensis subsp. tularensis.

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    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审


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    journal_title:Frontiers in cellular and infection microbiology

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    pub_type: 杂志文章


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    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章


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    journal_title:Frontiers in cellular and infection microbiology

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    pub_type: 杂志文章


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    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章


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  • Host Iron Nutritional Immunity Induced by a Live Yersinia pestis Vaccine Strain Is Associated with Immediate Protection against Plague.

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    pub_type: 杂志文章


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    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章


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    更新日期:2012-06-12 00:00:00

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    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审


    authors: Ganesan S,Sajjan US

    更新日期:2012-01-11 00:00:00

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    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章


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    更新日期:2020-06-16 00:00:00

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    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章


    authors: Zhou Y,Chen C,Yu H,Yang Z

    更新日期:2020-09-29 00:00:00

  • Gene Duplication and Protein Evolution in Tick-Host Interactions.

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    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审


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    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章,评审


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  • Porcine Reproductive and Respiratory Syndrome Virus Infection Induces Stress Granule Formation Depending on Protein Kinase R-like Endoplasmic Reticulum Kinase (PERK) in MARC-145 Cells.

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    journal_title:Frontiers in cellular and infection microbiology

    pub_type: 杂志文章


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    更新日期:2017-04-04 00:00:00