Abstract:
:We report here on the isolation of isogenic strains of Aeromonas hydrophila AB3 deleted for a segment of the aerolysin gene. All aer mutants obtained lacked the 49-kilodalton aerolysin gene product and were neither hemolytic for blood erythrocytes nor cytotoxic for Chinese hamster ovary tissue culture cells. One such mutant, AB3-5, was used in a mouse toxicity model to evaluate the role of aerolysin in the pathogenesis of A. hydrophila infections. The strain had a 50% lethal dose (LD50) of greater than 10(9) as compared with the parental strain which had an LD50 of 5 X 10(7). Reintegration of the deleted segment into AB3-5 resulted in an LD50 of 6 X 10(7) cells for this revertant. Furthermore, all mice injected with a sublethal dose of the parental strains developed necrotic lesions; this was never obtained with the aerolysin-deficient strain AB3-5. More importantly, specific neutralizing antibody to aerolysin was detected in mice surviving A. hydrophila infection, demonstrating that aerolysin is produced during the course of systemic A. hydrophila infections.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Chakraborty T,Huhle B,Hof H,Bergbauer H,Goebel Wdoi
10.1128/IAI.55.9.2274-2280.1987subject
Has Abstractpub_date
1987-09-01 00:00:00pages
2274-80issue
9eissn
0019-9567issn
1098-5522journal_volume
55pub_type
杂志文章abstract::In the present study, we have investigated the evolution and impact on virulence of a 350-kb genomic duplication present in the most recently evolved members of the Mycobacterium tuberculosis East Asian lineage. In a mouse model of infection, comparing HN878 subclones HN878-27 (no duplication) and HN878-45 (with the 3...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01791-14
更新日期:2014-07-01 00:00:00
abstract::Protozoan parasites of the genus Leishmania exist as obligatory intracellular amastigotes and invade macrophages and Langerhans cells, the dendritic cells of the skin. Langerhans cells are much more efficient in presenting Leishmania major antigen to T cells than macrophages are and have the unique ability to retain p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.8.3444-3450.1997
更新日期:1997-08-01 00:00:00
abstract::The ability of butyric acid, an extracellular metabolite from periodontopathic bacteria, to induce apoptosis in murine WEHI 231 cells, splenic B cells, and human RAJI cells was examined. The culture filtrate of Porphyromonas gingivalis, Prevotella loescheii, and Fusobacterium nucleatum, which contains high a percentag...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2587-2594.1998
更新日期:1998-06-01 00:00:00
abstract::Brucella organisms are intracellular stealth pathogens of animals and humans. The bacteria overcome the assault of innate immunity at early stages of an infection. Removal of polymorphonuclear neutrophils (PMNs) at the onset of adaptive immunity against Brucella abortus favored bacterial elimination in mice. This was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00118-19
更新日期:2019-04-23 00:00:00
abstract::Three MudJ prototrophs demonstrated that intracellular replication is a Salmonella virulence trait (K. Y. Leung and B. B. Finlay, Proc. Natl. Acad. Sci. USA, 88:11470-11474, 1991). mutS and mutH are disrupted in mutants 3-11 and 12-23, and ssaQ is disrupted in mutant 17-21. Further analysis revealed that loss of Salmo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.2247-2252.2003
更新日期:2003-04-01 00:00:00
abstract::Salmonella enterica subspecies I serovars are common bacterial pathogens causing diseases ranging from enterocolitis to systemic infections. Some serovars are adapted to specific hosts, whereas others have a broad host range. The molecular mechanisms defining the virulence characteristics and the host range of a given...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.1.632-644.2006
更新日期:2006-01-01 00:00:00
abstract::Neisseria meningitidis crosses the blood-brain barrier (BBB) following the activation of the β2-adrenergic receptor by the type IV pili (TFP). Two components of the type IV pili recruit the β2-adrenergic receptor, the major pilin PilE and the minor pilin PilV. Here, we report that a strain deleted of PilX, one of the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00369-12
更新日期:2012-09-01 00:00:00
abstract::Autotransporters of gram-negative bacteria are single-peptide secretion systems that consist of a functional N-terminal alpha-domain ("passenger") fused to a C-terminal beta-domain ("translocator"). How passenger proteins are translocated through the outer membrane has not been resolved, and at present essentially thr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.7.3851-3859.2005
更新日期:2005-07-01 00:00:00
abstract::Antiprotein monoclonal antibodies derived from mice inoculated with Rickettsia rickettsii heated at 56 degrees C for 15 min are of two types: one is type specific for epitopes denatured by moderate temperatures, and the other is specific for epitopes resistant to 100 degrees C for 5 min. The heat-resistant epitopes ar...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.825-827.1987
更新日期:1987-03-01 00:00:00
abstract::The antigenicity of Erysipelothrix rhusiopathiae T28 (serotype 2) was investigated. Antigens were solubilized from the cell surface with detergents. By means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting it was shown that the molecular weight of the main antigenic component--a nonprot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.3.818-822.1986
更新日期:1986-06-01 00:00:00
abstract::Coxiella burnetii, phase I and II, cells cultivated in the yolk sac of chicken embryos were separated from host cell components by two cycles of isopycnic Renografin gradient centrifugation. Initial steps in the purification of viable C. burnetii involved differential centrifugation and sedimentation through an aqueou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.2.840-851.1981
更新日期:1981-05-01 00:00:00
abstract::The extracellular cysteine protease from Streptococcus pyogenes is a virulence factor that plays a significant role in host-pathogen interaction. Streptococcal protease is expressed as an inactive 40-kDa precursor that is autocatalytically converted into a 28-kDa mature (active) enzyme. Replacement of the single cyste...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4326-4333.1999
更新日期:1999-09-01 00:00:00
abstract::Coagulation and inflammation are interconnected, suggesting that coagulation plays a key role in the inflammatory response to pathogens. A phenome-wide association study (PheWAS) was used to identify clinical phenotypes of patients with a polymorphism in coagulation factor X. Patients with this single nucleotide polym...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00031-19
更新日期:2019-04-23 00:00:00
abstract::It was shown in this work that the infectivity of metacyclic forms of Trypanosoma cruzi was affected upon interaction with the monoclonal antibody (10D8), which reacts with a carbohydrate epitope of the 35- and 50-kilodalton (kDa) surface glycoconjugates. The invasion of Vero cells by metacyclic forms of strains Tulah...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.6.1663-1667.1989
更新日期:1989-06-01 00:00:00
abstract::Studies were carried out to determine whether the in vitro capacity of adherent peritoneal cells to replicate herpes simplex virus type 1 (HSV-1) might correlate with the in vivo susceptibility of mice genetically resistant, moderately susceptible, or very susceptible to HSV-1 infection. Unstimulated and proteose pept...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.2.432-437.1979
更新日期:1979-02-01 00:00:00
abstract::We compared the immune defense of mice with chronic granulomatous disease (CGD mice) with that of wild-type C57BL/6 mice for their response to Sporothrix schenckii. A subcutaneous injection of 5 x 10(4) CFU S. schenckii strain IFM41598 into CGD mice resulted in systemic infection and death within 84 days. In contrast,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5073-5079.2004
更新日期:2004-09-01 00:00:00
abstract::Administration of free muramyl tripeptide phosphatidylethanolamide (MTPPE) or liposome-encapsulated MTPPE (LE-MTPPE) in a twofold-lower dose at 24 h before bacterial inoculation resulted in clearance of intravenously inoculated Klebsiella pneumoniae by tissue macrophages, whereas in control mice, bacteria were not eff...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4891-4897.1992
更新日期:1992-11-01 00:00:00
abstract::Hematogenous infections caused by Candida krusei have been noted with increasing frequency, particularly in cancer patients receiving prophylaxis with antifungal triazoles. Progress in understanding the pathogenesis of this emerging infection has been limited by the lack of an animal model. We developed a CF1 mouse in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.4.1268-1271.1993
更新日期:1993-04-01 00:00:00
abstract::The effects of passive administration of neutralizing monoclonal antibodies (MAB) to respiratory syncytial virus glycoproteins (GP90 and VP70) was evaluated in cotton rats challenged with respiratory syncytial virus. Animals injected with MAB to VP70 had lower mean viral titers in lung tissues than did controls (log10...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.2.756-758.1984
更新日期:1984-02-01 00:00:00
abstract::The interaction of the first component of complement with two serum-sensitive strains of Escherichia coli and Klebsiella pneumoniae was studied. It could be demonstrated that highly purified C1, free of immunoglobulin G and immunoglobulin M, binds to E. coli or K. pneumoniae. C1 binding was also found with specificall...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.1.5-9.1978
更新日期:1978-10-01 00:00:00
abstract::Streptococcus pneumoniae is a major cause of illness and death in children in developing countries. In these children, zinc deficiency is associated with an increased risk of acute respiratory tract infections, which can be reduced by daily zinc administration. Severe infections decrease zinc levels in plasma and may ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.2009-2013.2003
更新日期:2003-04-01 00:00:00
abstract::Neuronal dysfunction can occur in the course of sepsis without meningitis. Sepsis-associated neuronal damage (SAND) was observed in the hippocampus within hours in experimental pneumococcal bacteremia. Intravascular challenge with purified bacterial cell wall recapitulated SAND. SAND persisted in PAFr(-/-) mice but wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00022-06
更新日期:2006-07-01 00:00:00
abstract::Mutation of sarA resulted in a reduced capacity to form a biofilm in six of the eight Staphylococcus aureus strains we tested (UAMS-1, UAMS-601, SA113, SC-01, S6C, and DB). The exceptions were Newman, which formed a poor biofilm under all conditions, and RN6390, which consistently formed a biofilm only after mutation ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.4206-4211.2003
更新日期:2003-07-01 00:00:00
abstract::The Staphylococcus aureus repeat (STAR) element is a sequence identified in two intergenic regions in S. aureus. The element is found in 13 to 21 copies in individual S. aureus strains, and elements in the homologous intergenic location are variable in length. The element sequence consists of several small and unusual...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2344-2348.2000
更新日期:2000-04-01 00:00:00
abstract::Yersinia pestis evolved from Y. pseudotuberculosis to become the causative agent of bubonic and pneumonic plague. We identified a homolog of the Salmonella enterica serovar Typhimurium lipoprotein (lpp) gene in Yersinia species and prepared lpp gene deletion mutants of Y. pseudotuberculosis YPIII, Y. pestis KIM/D27 (p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01529-07
更新日期:2008-04-01 00:00:00
abstract::Exogenous ammonium ions (NH(4) (+)) and amine compounds had a profound influence on the antibacterial activity of the myeloperoxidase-hydrogen peroxide-chloride system against Escherichia coli. The rate of killing increased in the presence of NH(4) (+) and certain guanidino compounds and decreased in the presence of a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.1.110-116.1979
更新日期:1979-07-01 00:00:00
abstract::In a model of experimental Chagas' disease induced with metacyclic forms of Trypanosoma cruzi, CD4+ but not CD8+ T cells undergo T-cell receptor (TCR)-CD3-mediated activation-induced cell death (AICD) in vitro. CD4+ T cells from T. cruzi-infected mice also developed unresponsiveness in proliferative responses to TCR-C...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.5.1559-1564.1996
更新日期:1996-05-01 00:00:00
abstract::Arg-gingipain (Rgp) and Lys-gingipain (Kgp) are Porphyromonas gingivalis cysteine proteinases implicated as major virulence factors in pathologies of periodontitis. We purified a 660-kDa cell-associated gingipain complex existing as a homodimer of two catalytically active monomers which comprises their catalytic and a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.2.883-893.2005
更新日期:2005-02-01 00:00:00
abstract::Peroxidase-conjugated antibodies were found to be as sensitive as those conjugated to fluorescein-isothiocyanate (FITC) for identification of selected enterovirus types by the indirect technique. Peroxidase conjugates, however, were found to give fewer nonspecific reactions. The reasons for the higher specificity of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.220-226.1974
更新日期:1974-07-01 00:00:00
abstract::Lipopolysaccharide (LPS) is one of the most potent trigger substances for monocytes and macrophages causing secretion of tumor necrosis factor alpha (TNF-alpha) and other inflammatory mediators. The nature of the nuclear factors involved in human TNF-alpha gene regulation is still unknown. Nuclear factor kappa B (NF-k...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1529-1534.1995
更新日期:1995-04-01 00:00:00