Formation and regeneration of a Wnt-responsive junctional epithelium.

Abstract:

AIM:To identify the molecular mechanisms mediating the persistent defensive functions of the self-renewing junctional epithelium (JE). MATERIALS AND METHODS:Two strains of Wnt reporter mice, Axin2CreErt2/+ ;R26RmTmG/+ and Axin2LacZ/+ , were employed, along with three clinically relevant experimental scenarios where the function of the JE is disrupted: after tooth extraction, after a partial gingivectomy, and after a complete circumferential gingivectomy. RESULTS:Using transgenic Wnt reporter strains of mice, we established the JE is a Wnt-responsive epithelium beginning at the time of its formation and that it maintains this status into adulthood. After tooth extraction, progeny of the initial Wnt-responsive JE population directly contributed to healing and ultimately adopted an oral epithelium (OE) phenotype. In the traditional partial gingivectomy model, the JE completely regenerated and did so via progeny of the original Wnt-responsive population. However, following circumferential gingivectomy, the OE was incapable of re-establishing a functional JE. CONCLUSIONS:A Wnt-responsive niche at the interface between tooth and oral epithelia is required for a functional JE.

journal_name

J Clin Periodontol

authors

Yuan X,Chen J,Van Brunt LA,Grauer J,Xu Q,Pei X,Wang L,Zhao Y,Helms JA

doi

10.1111/jcpe.13371

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

1476-1484

issue

12

eissn

0303-6979

issn

1600-051X

journal_volume

47

pub_type

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