Abstract:
:Both transforming growth factor-beta (TGF-beta) and platelet-derived growth factor (PDGF) have been shown to affect cell proliferation in vitro. The hypothesis being tested was that the effects of the 2 cytokines would be modulated by the presence of serum in the medium. Gingival fibroblasts, obtained from periodontally healthy patients, were maintained in primary culture. Dose response experiments were performed for each growth factor in serum-free medium and in medium containing natural or heat-inactivated fetal bovine serum (10% FBS). Changes in cell numbers were quantified by crystal violet staining. The optimal concentrations of the individual factors (10 ng/ml TGF-beta1, 20 ng/ ml PDGF-BB) were then used when the 2 factors were tested in various sequences. In serum-free medium or in medium with 10% natural serum, the response to PDGF-BB was dose-dependent up to 40 ng/ml; however, with 10% heat-inactivated serum, the maximal response was seen at 20 ng/ml. The largest increase in cell numbers was produced by the simultaneous exposure to the two cytokines, rather than a sequential presentation. The findings suggest that the 48-h growth response of human gingival fibroblasts to 10 ng/ml TGF-beta1 or 20 ng/ ml PDGF-BB in serum-free medium was equivalent to growth obtained in medium containing heat-inactivated 10% FBS without added growth factors.
journal_name
J Clin Periodontoljournal_title
Journal of clinical periodontologyauthors
Anderson TJ,Lapp CA,Billman MA,Schuster GSdoi
10.1111/j.1600-051x.1998.tb02363.xsubject
Has Abstractpub_date
1998-01-01 00:00:00pages
48-55issue
1eissn
0303-6979issn
1600-051Xjournal_volume
25pub_type
杂志文章abstract:AIM:The aim of the present experiment was to study if the quality of the titanium surface is a decisive factor for osseointegration and re-osseointegration. MATERIAL AND METHODS:2 Labrador dogs were used. The mandibular 1st molars and all premolars were removed bilaterally. 3 months later, 1 standard fixture and 3, 2-...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1034/j.1600-051x.2001.028003258.x
更新日期:2001-03-01 00:00:00
abstract:AIM:To analyse, through a pre-clinical in vivo model, the possible mechanisms linking depression and periodontitis at behavioural, microbiological and molecular levels. MATERIALS AND METHODS:Periodontitis (P) was induced in Wistar:Han rats (oral gavages with Porphyromonas gingivalis and Fusobacterium nucleatum) during...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/jcpe.13420
更新日期:2021-01-12 00:00:00
abstract:BACKGROUND, AIMS:The form of the molar roots and their furcated region influence both the pathogenesis of periodontal destruction and therapeutic attempts. METHOD:Using a specially-constructed measuring device, 359 1st and 2nd molars, both upper and lower, were analysed 3-dimensionally. The parameters measured were ho...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1034/j.1600-051x.2000.027012926.x
更新日期:2000-12-01 00:00:00
abstract::The purpose of this study was to assess the effect of rinsing and topical application of sanguinarine on labial surface plaque accumulation, compared with topically applied water, and compared with rinsing with chlorhexidine, while refraining from all oral hygiene measures for 5 days. Color photographs of the disclose...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1988.tb01002.x
更新日期:1988-02-01 00:00:00
abstract::Thirty-six subjects with no evidence of clinical gingival inflammation underwent a standardized toothbrushing procedure. Blood specimens, obtained from a vein in the antecubital fossa during the last 30 seconds of brushing, were cultured under aerobic and stringent anaerobic conditions. Three subjects exhibited detect...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1979.tb02288.x
更新日期:1979-02-01 00:00:00
abstract:OBJECTIVES:The pharmacodynamic properties of ibuprofen are related nearly exclusively to the S(+)enantiomer (dexibuprofen). This study investigated the effect of a 1.5% dexibuprofen mouth rinse in an experimentally induced gingivitis. MATERIALS AND METHODS:The trial was a randomized, double-blinded, placebo-controlled...
journal_title:Journal of clinical periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1600-051X.2005.00721.x
更新日期:2005-06-01 00:00:00
abstract:AIM:To identify the molecular mechanisms mediating the persistent defensive functions of the self-renewing junctional epithelium (JE). MATERIALS AND METHODS:Two strains of Wnt reporter mice, Axin2CreErt2/+ ;R26RmTmG/+ and Axin2LacZ/+ , were employed, along with three clinically relevant experimental scenarios where th...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/jcpe.13371
更新日期:2020-12-01 00:00:00
abstract::Early plaque growth and plaque topography were studied in 15 young adults with a healthy periodontium during a 4-day period of no oral hygiene. After perfect tooth-cleaning, the recolonisation of the buccal surfaces of 48 teeth (front teeth and premolars) was recorded at the start and after 6, 12, 24, 30, 36, 48, 54, ...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1989.tb01655.x
更新日期:1989-05-01 00:00:00
abstract:BACKGROUND:The aim of this study was to report on the prevalence of Actinobacillus actinomycetemcomitans (Aa) and the periodontal clinical conditions in children and adolescents from a rural area of central Italy compared with the ones from an urban area of the same region. METHOD:The study population consisted of 780...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1034/j.1600-051x.2000.027008549.x
更新日期:2000-08-01 00:00:00
abstract::The purpose of the study was to examine the association between alignment conditions of anterior teeth and dental health in 15-year-old adolescents. The study group consisted of 74 females and 70 males with 6 anterior teeth in both jaws. The following parameters were assessed: plaque index (P1I), gingival index (GI), ...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1985.tb02297.x
更新日期:1985-04-01 00:00:00
abstract:BACKGROUND:Peri-implant soft tissue may be critical to prevent inflammation and promote gingival margin stability. The purpose of this randomized clinical trial (RCT) is to compare xenogeneic collagen matrix (XCM) versus connective tissue graft (CTG) to increase buccal soft tissue thickness at implant site. MATERIALS ...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章,随机对照试验
doi:10.1111/jcpe.12750
更新日期:2017-07-01 00:00:00
abstract:AIM:There is a paucity of up-to-date data regarding prevalence and risk indicators of periodontitis in Italy. Therefore, the aim of this study was to evaluate the prevalence of periodontitis and its risk indicators among adults from an urban area in North Italy. MATERIAL AND METHODS:This cross-sectional survey used a ...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/jcpe.12420
更新日期:2015-07-01 00:00:00
abstract::The purpose of this study was to investigate the validity and reproducibility of an oral rating index (ORI) as a conceptual measurement of "gingival health care" level in adults. The study was conducted on 163 patients who received medical examinations and attended for dental checkups at a hospital. The score of the O...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1034/j.1600-051x.2000.027006411.x
更新日期:2000-06-01 00:00:00
abstract:PRIMARY OBJECTIVE:To evaluate a multicentre clinical trial design for testing powered toothbrushes. SECONDARY OBJECTIVE:To compare the efficacy of a prototype brush head (N2.3) for the Philips Jordan Sensiflex 2000 powered toothbrush (PTB) to that of Braun Oral-B D15 PTB in removing dental plaque MATERIAL AND METHODS...
journal_title:Journal of clinical periodontology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1034/j.1600-051x.2002.291003.x
更新日期:2002-10-01 00:00:00
abstract::The purpose of the present investigation was to explore possible relationships between clinical periodontal status in periodontally involved teeth with and without endodontic infection. The investigation was conducted as a retrospective study on a consecutive referral population. The periapical conditions in endodonti...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1993.tb00325.x
更新日期:1993-02-01 00:00:00
abstract::The present investigation was performed in order to study the reaction of the gingiva in the deciduous and permanent dentition of dogs to 3 weeks of plaque accumulation. The study was carried out in 10 beagles, divided into 2 groups of 5 dogs each; group I and group II. When the dogs of group I were 10 weeks old, a me...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1989.tb01675.x
更新日期:1989-08-01 00:00:00
abstract::The possible effects of ultrasonic scaling of all maxillary teeth for a total of 5 min on the inner ears of 20 healthy young adults, 22-36 years of age, were investigated by means of pure tone audiometry. Audiograms were obtained just before and immediatley after scaling. Temporary shifts in hearing threshold (TTS) of...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1976.tb01858.x
更新日期:1976-05-01 00:00:00
abstract:BACKGROUND:Treatment of localized aggressive periodontitis (LAP) may include systemic antibiotics, yet it is unclear at what stage of treatment planning antibiotics are most effective. AIM:This retrospective analysis compared immediate versus delayed antibiotic therapy on clinical parameters and gingival crevicular fl...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/jcpe.12001
更新日期:2012-11-01 00:00:00
abstract::The purpose of the present investigation was to test the concept that a dentifrice containing zinc citrate and Triclosan could maintain gingival health. The gingival health of 101 young predominately female student nurses in Oslo was brought to a high level by professional cleaning supported by oral hygiene instructio...
journal_title:Journal of clinical periodontology
pub_type: 临床试验,杂志文章
doi:10.1111/j.1600-051x.1987.tb02251.x
更新日期:1987-09-01 00:00:00
abstract::In a cross-sectional, epidemiological study of phenytoin induced gingival overgrowth in 77 institutionalized persons with epilepsy, the severity of the gum lesions was quantified by means of a precise new technique. Lesion severity was then compared statistically to other clinical and laboratory parameters. Positive c...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1984.tb02214.x
更新日期:1984-04-01 00:00:00
abstract::The present investigation was designed to assess the effects of strips made of different materials on the recovery of enzymes in gingival crevicular fluid (GCF) (Experiment 1), and to examine a possible relationship between lysosomal enzyme activities and number of crevicular polymorphonuclear leukocytes (PMNs) (Exper...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1994.tb00411.x
更新日期:1994-08-01 00:00:00
abstract:AIM:To evaluate the local immunoinflammatory profiles in localized aggressive periodontitis patients (LAP) before and after periodontal treatment and maintenance. METHODS:Sixty-six African-Americans with LAP (7-21 years old) were included. After periodontal examination, all patients received periodontal treatment with...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/jcpe.13404
更新日期:2021-02-01 00:00:00
abstract:BACKGROUND:The aim of this randomized clinical trial was to evaluate the efficacy of Apically Positioned Flap with Fibre Retention Osseous Resective Surgery (FibReORS) or Osseous Resective Surgery (ORS) to treat periodontal pockets associated with infrabony defect ≤3 mm at posterior natural teeth. MATERIAL AND METHODS...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章,随机对照试验
doi:10.1111/jcpe.12042
更新日期:2013-02-01 00:00:00
abstract::This comparative clinical 9-month study was designed to examine the efficacy of amine/stannous fluoride (AmF/SnF2) (Meridol) and sodium fluoride (NaF). 150 probands with chronic gingivitis or early signs of periodontitis were divided into 3 randomised groups. Group 1 was given a NaF toothpaste and an NaF mouthrinse, g...
journal_title:Journal of clinical periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1600-051x.1996.tb00560.x
更新日期:1996-04-01 00:00:00
abstract::This study examines the incidence and severity of postoperative pain after gingivectomy using one non-eugenol-containing periodontal dressing, Coe-pak (n = 76) and 2 eugenol-containing periodontal dressings, Wondrpak (n = 64) and Nobetec (n = 86). All patients were subjected to gingivectomy using 1 type of local anaes...
journal_title:Journal of clinical periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1600-051x.1990.tb00028.x
更新日期:1990-07-01 00:00:00
abstract:AIM:To develop and validate a condition specific measure of oral health-related quality of life for dentine hypersensitivity (Dentine Hypersensitivity Experience Questionnaire, DHEQ). MATERIALS AND METHODS:Questionnaire construction used a multi-staged impact approach and an explicit theoretical model. Qualitative and...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051X.2010.01618.x
更新日期:2010-11-01 00:00:00
abstract:OBJECTIVE:To assess the efficacy of the novel ultrasonic Vector -system system for subgingival debridement and to compare the results with conventional periodontal instrumentation in vitro and in vivo. MATERIAL AND METHODS:Forty extracted human teeth were treated in vitro: Vector -system with polishing (VP) and abrasi...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1600-051X.2006.00960.x
更新日期:2006-08-01 00:00:00
abstract:OBJECTIVES:Thrombotic thrombocytopenic purpura (TTP) is a rare haematological disease of unknown aetiology. This thrombotic microangiopathy is characterized by microvascular lesions with platelet aggregation. It is found in adults and can be associated with pregnancy, cancer, autoimmune diseases, bone marrow transplant...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051X.2004.00617.x
更新日期:2004-11-01 00:00:00
abstract:BACKGROUND:Only a few dental procedures have been reported to cause life-threatening bleeding. All of these cases followed surgical intervention. MATERIAL AND METHODS:In this paper, we report a case of severe bleeding following non-surgical periodontal procedures in a patient treated with a dual anti-platelet regimen ...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051X.2008.01206.x
更新日期:2008-04-01 00:00:00
abstract:AIM:To obtain salivary interleukin (IL) 1β-based models to predict the probability of the occurrence of periodontitis, differentiating by smoking habit. MATERIALS/METHODS:A total of 141 participants were recruited, 62 periodontally healthy controls and 79 subjects affected by periodontitis. Fifty of the diseased patie...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/jcpe.13285
更新日期:2020-06-01 00:00:00