The recovery efficiency of various materials for sampling enzymes and polymorphonuclear leukocytes from gingival crevices.

Abstract:

:The present investigation was designed to assess the effects of strips made of different materials on the recovery of enzymes in gingival crevicular fluid (GCF) (Experiment 1), and to examine a possible relationship between lysosomal enzyme activities and number of crevicular polymorphonuclear leukocytes (PMNs) (Experiment 2). In Experiment 1, GCF was collected with capillaries from 14 patients with periodontal disease, and applied on various test strips in microcentrifuged tubes or directly into tubes (controls). Strips were then shaken and centrifuged to elute GCF enzymes. The supernate was used for determinations of alkaline phosphatase (ALP), beta-glucuronidase (BG) and aspartate aminotransferase (AST) activities. The % recoveries were calculated as relative percentages to controls. When using saline as eluent, 90% or more ALP, BG and AST were found to be recovered from strips of durapore and papers. More than 35% of ALP and BG was found to remain on paper points. However, the % recoveries from paper points were improved using eluent with 0.1% (w/v) cetyl pyridinium chloride. In Experiment 2, 54 GCF samples were collected from 3 periodontitis patients by using durapore strips, in order to measure both PMN numbers and BG activities in the same samples. The 2 parameters showed strong and positive correlation with 0.847 (p < 0.001) of the Pearson's correlation coefficient. These findings suggest that durapore is a useful material not only for counting PMNs but also for measuring activities of GCF enzymes. Elevation of BG activities in GCF can be due to increasing numbers of PMNs.

journal_name

J Clin Periodontol

authors

Nakashima K,Demeurisse C,Cimasoni G

doi

10.1111/j.1600-051x.1994.tb00411.x

subject

Has Abstract

pub_date

1994-08-01 00:00:00

pages

479-83

issue

7

eissn

0303-6979

issn

1600-051X

journal_volume

21

pub_type

杂志文章
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    doi:10.1111/j.1600-051X.2004.00502.x

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    doi:10.1111/j.1600-051x.1994.tb00784.x

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    doi:10.1111/j.1600-051X.2004.00629.x

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    更新日期:2005-01-01 00:00:00

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    pub_type: 临床试验,杂志文章,随机对照试验

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    更新日期:1986-03-01 00:00:00

  • Epithelial remnants in the crestal periodontium of the deciduous and permanent dentition of beagle dogs.

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    pub_type: 杂志文章

    doi:10.1111/j.1600-051x.1994.tb00754.x

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    更新日期:1994-10-01 00:00:00

  • The role of RgpA in the pathogenicity of Porphyromonas gingivalis in the murine periodontitis model.

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    doi:10.1111/jcpe.12139

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    更新日期:2013-10-01 00:00:00

  • MMP3 and TIMP1 variants contribute to chronic periodontitis and may be implicated in disease progression.

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  • Plaque removal by dental floss or toothpicks. An intra-individual comparative study.

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    doi:10.1111/j.1600-051x.1980.tb02158.x

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    更新日期:1980-12-01 00:00:00

  • Comparison between polymerase chain reaction-based and checkerboard DNA hybridization techniques for microbial assessment of subgingival plaque samples.

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    pub_type: 杂志文章

    doi:10.1111/j.1600-051X.2009.01434.x

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    pub_type: 杂志文章

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  • Microscopic spirochete counts in untreated subjects with and without periodontal tissue destruction.

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    doi:10.1111/j.1600-051x.1995.tb00140.x

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    更新日期:1995-03-01 00:00:00

  • Antagonists of Wnt/β-catenin signalling in the periodontitis associated with type 2 diabetes and smoking.

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    pub_type: 杂志文章

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  • Increased release of elastase from in vitro activated peripheral neutrophils in patients with adult periodontitis.

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    journal_title:Journal of clinical periodontology

    pub_type: 杂志文章

    doi:10.1034/j.1600-051x.1999.260402.x

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    更新日期:1999-04-01 00:00:00

  • A systematic review on the effects of local antimicrobials as adjuncts to subgingival debridement, compared with subgingival debridement alone, in the treatment of chronic periodontitis.

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    journal_title:Journal of clinical periodontology

    pub_type: 杂志文章,meta分析,评审

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  • Post-extraction dimensional changes: A systematic review and meta-analysis.

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  • Benefits of early systemic antibiotics in localized aggressive periodontitis: a retrospective study.

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    更新日期:2012-11-01 00:00:00

  • A study to assess the plaque inhibitory action of a new zinc citrate toothpaste formulation.

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    pub_type: 临床试验,杂志文章,随机对照试验

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  • Root defects following air polishing.

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    pub_type: 杂志文章

    doi:10.1034/j.1600-051x.2003.300204.x

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    更新日期:2003-02-01 00:00:00

  • The comparative effect of acidified sodium chlorite and chlorhexidine mouthrinses on plaque regrowth and salivary bacterial counts.

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    pub_type: 临床试验,杂志文章,随机对照试验

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    authors: Yates R,Moran J,Addy M,Mullan PJ,Wade WG,Newcombe R

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  • A papillary-verrucous lesion in the gingiva, developing into squamous cell carcinoma.

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    doi:10.1111/j.1600-051x.1987.tb01515.x

    authors: Helldén L,Jonsson R

    更新日期:1987-01-01 00:00:00

  • Periodontitis and systemic diseases: a record of discussions of working group 4 of the Joint EFP/AAP Workshop on Periodontitis and Systemic Diseases.

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    更新日期:2013-04-01 00:00:00

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  • Phenytoin induced gingival overgrowth in institutionalized epileptics.

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  • Concepts determining statistical analysis of dental data.

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