Abstract:
:The present investigation was designed to assess the effects of strips made of different materials on the recovery of enzymes in gingival crevicular fluid (GCF) (Experiment 1), and to examine a possible relationship between lysosomal enzyme activities and number of crevicular polymorphonuclear leukocytes (PMNs) (Experiment 2). In Experiment 1, GCF was collected with capillaries from 14 patients with periodontal disease, and applied on various test strips in microcentrifuged tubes or directly into tubes (controls). Strips were then shaken and centrifuged to elute GCF enzymes. The supernate was used for determinations of alkaline phosphatase (ALP), beta-glucuronidase (BG) and aspartate aminotransferase (AST) activities. The % recoveries were calculated as relative percentages to controls. When using saline as eluent, 90% or more ALP, BG and AST were found to be recovered from strips of durapore and papers. More than 35% of ALP and BG was found to remain on paper points. However, the % recoveries from paper points were improved using eluent with 0.1% (w/v) cetyl pyridinium chloride. In Experiment 2, 54 GCF samples were collected from 3 periodontitis patients by using durapore strips, in order to measure both PMN numbers and BG activities in the same samples. The 2 parameters showed strong and positive correlation with 0.847 (p < 0.001) of the Pearson's correlation coefficient. These findings suggest that durapore is a useful material not only for counting PMNs but also for measuring activities of GCF enzymes. Elevation of BG activities in GCF can be due to increasing numbers of PMNs.
journal_name
J Clin Periodontoljournal_title
Journal of clinical periodontologyauthors
Nakashima K,Demeurisse C,Cimasoni Gdoi
10.1111/j.1600-051x.1994.tb00411.xsubject
Has Abstractpub_date
1994-08-01 00:00:00pages
479-83issue
7eissn
0303-6979issn
1600-051Xjournal_volume
21pub_type
杂志文章abstract:OBJECTIVES:Aim of the study was to evaluate the predictive value of the modified periodontal risk assessment (PRA) in patients with aggressive periodontitis (AgP) for the first time. MATERIAL AND METHODS:A total of 86 Patients with AgP were re-examined 5-17 years after active periodontal treatment. Risk profile accord...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051X.2012.01895.x
更新日期:2012-07-01 00:00:00
abstract:BACKGROUND:It was demonstrated that a marginal defect of about 1 mm between the bone wall and the metal surface after implant installation can heal with a high degree of bone fill and osseointegration. OBJECTIVE:The aim of the present animal experiment was to study bone healing at implant sites with hard tissue defect...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051X.2004.00502.x
更新日期:2004-04-01 00:00:00
abstract::Several authors have determined clinically the width of attached gingiva (AG) over the deciduous and early permanent dentitions in children. They have noticed that when comparing the width of AG over deciduous teeth to the width of AG over the succeeding newly erupted permanent teeth, a clear diminishing in AG can be ...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1994.tb00784.x
更新日期:1994-11-01 00:00:00
abstract::Deposits of dental plaque or calculus are typically quantified using planimetric techniques. However, error is introduced into a quantitative analysis of plaque deposits using these methods, since they require a significant amount of human intervention. The purpose of this study is to describe and validate a computeri...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1992.tb00683.x
更新日期:1992-09-01 00:00:00
abstract::During a clinical trial for evaluating the antiplaque effect of two flavoured chlorhexidine rinses, the observation was made that bleeding after gentle massage of the gingival margin occurred more often after chemical than after mechanical oral hygiene measures. In order to ascertain the validity of this unexpected ob...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1982.tb02100.x
更新日期:1982-07-01 00:00:00
abstract:BACKGROUND:In this study, risk determinants were determined for periodontal disease in the representative population sample (n=3146) of the Study of Health in Pomerania. METHODS:After examining the net random sample (response 69%) and exclusion of edentulous cases and those with missing values, 2595 subjects remained....
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051X.2004.00629.x
更新日期:2005-01-01 00:00:00
abstract::23 patients with pockets greater than or equal to 4 mm and evidence of bone loss on radiographs received thorough scaling and root planing followed by instruction in Bass brushing. They refrained from routine interdental cleaning for the first 28 days. A pulsating jet irrigator was used once daily supragingivally with...
journal_title:Journal of clinical periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1600-051x.1986.tb01466.x
更新日期:1986-03-01 00:00:00
abstract::The purpose of this study was to document and characterize epithelial remnants (EPRs) of the crestal periodontium of the deciduous dentition of a diphyodont and compare them with EPR units found in the corresponding area of the permanent dentition. 7 beagle dogs were used. At the age of 10 weeks (deciduous dentition) ...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1994.tb00754.x
更新日期:1994-10-01 00:00:00
abstract:AIM:To investigate the in vivo role of gingipains in Porphyromonas gingivalis' virulence, and suggest a possible host mechanisms through which the bacteria cause alveolar bone loss. MATERIALS AND METHODS:Mice were orally infected with P. gingivalis wild type, or the gingipains mutants (RgpA⁻, Kgp⁻, RgpA⁻/Kgp⁻). Mice w...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/jcpe.12139
更新日期:2013-10-01 00:00:00
abstract:AIM:Matrix metalloproteinases (MMPs) play a key role in the tissue destruction characteristic of chronic periodontitis. The purpose of this study was to investigate the association of MMP and TIMP polymorphisms with chronic periodontitis in two populations. MATERIAL AND METHODS:A total of 34 polymorphisms spanning 12 ...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051X.2012.01902.x
更新日期:2012-08-01 00:00:00
abstract::The amount of plaque accumulation was assessed in an intraindividual study comprising 10 individuals. During different 2-week periods, the test subjects used nylon floss (unwaxed, waxed and specially treated), silk floss (unwaxed and waxed), Superfloss or triangular toothpicks for interdental tooth cleaning. Only teet...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1980.tb02158.x
更新日期:1980-12-01 00:00:00
abstract:AIM:To compare polymerase chain reaction (PCR) with subsequent reverse hybridization (micro-IDent test) and checkerboard DNA-DNA hybridization for the identification of 13 bacterial species in subgingival plaque samples. MATERIAL AND METHODS:Subgingival plaque samples were taken using paper points and curettes from tw...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051X.2009.01434.x
更新日期:2009-08-01 00:00:00
abstract:AIM:To study the effect of the dose and type of calcium channel blockers (CCBs) on the risk of gingival hyperplasia and to quantify this association. METHODS:The study was conducted within the Integrated Primary Care Information Project in The Netherlands. A nested case-control study was designed within a cohort of al...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051X.2010.01574.x
更新日期:2010-07-01 00:00:00
abstract::The purpose of the study was to determine microscopically the %s of spirochetes at sites without periodontal destruction in subjects with destructive periodontal diseases (cases) and in subjects free of it (controls), who had not received professional prophylaxis. From a sample of 164 individuals aged between 30-44 ye...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1995.tb00140.x
更新日期:1995-03-01 00:00:00
abstract:AIM:This study evaluated the levels of sclerostin (SOST) and Dickkopf (DKK)-1 in the chronic periodontitis (CP) associated with type 2 diabetes (DM) and/or smoking. Relationships between SOST, DDK1, RANKL, OPG, IL-1ß, IL-6 and TNF-α, and pathogens were assessed. MATERIAL AND METHODS:The study population included non-d...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/jcpe.12854
更新日期:2018-03-01 00:00:00
abstract::The main object of this study was to determine if there was a difference between patients with adult periodontitis and healthy controls in the release of elastase. We also wanted to test the release of alpha-1-antitrypsin and lactoferrin from in vitro-activated peripheral neutrophils. A leukocyte-rich preparation from...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1034/j.1600-051x.1999.260402.x
更新日期:1999-04-01 00:00:00
abstract:AIMS:To update the existing scientific evidence on the efficacy of local antimicrobials as adjuncts to subgingival debridement in the treatment of chronic periodontitis. MATERIAL AND METHODS:Fifty-six papers were selected, reporting data from 52 different investigations. All the studies reported changes in probing poc...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章,meta分析,评审
doi:10.1111/jcpe.12026
更新日期:2013-03-01 00:00:00
abstract:AIM:To analyse the evidence pertaining to post-extraction dimensional changes in the alveolar ridge after unassisted socket healing. MATERIALS AND METHODS:The protocol of this PRISMA-compliant systematic review (SRs) was registered in PROSPERO (CRD42020178857). A literature search to identify studies that fulfilled th...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章,评审
doi:10.1111/jcpe.13390
更新日期:2021-01-01 00:00:00
abstract:BACKGROUND:Treatment of localized aggressive periodontitis (LAP) may include systemic antibiotics, yet it is unclear at what stage of treatment planning antibiotics are most effective. AIM:This retrospective analysis compared immediate versus delayed antibiotic therapy on clinical parameters and gingival crevicular fl...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/jcpe.12001
更新日期:2012-11-01 00:00:00
abstract:BACKGROUND, AIMS:Zinc-citrate-containing toothpastes have previously been suggested to be of value at inhibiting plaque and gingival inflammation. Present formulations have included triclosan which is thought to contribute major antibacterial/antiplaque effects. Alternative antimicrobial agents and other ingredients su...
journal_title:Journal of clinical periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1034/j.1600-051x.2001.028002157.x
更新日期:2001-02-01 00:00:00
abstract:AIM:Air-polishing devices (APDs) are highly effective in removing plaque and extrinsic staining. Their application on root surfaces, however, may result in clinically relevant substance removal, limiting the use in patients with periodontitis, where denuded root surfaces are frequently found. Therefore, the purpose of ...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1034/j.1600-051x.2003.300204.x
更新日期:2003-02-01 00:00:00
abstract::Acidified sodium chlorite (ASC) is recognised as a highly potent, broad spectrum antimicrobial system that has been successfully developed for uses in veterinary, food processing and medical device fields. The current studies aimed to investigate the persistence of antimicrobial action and plaque inhibitory properties...
journal_title:Journal of clinical periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1600-051x.1997.tb00236.x
更新日期:1997-09-01 00:00:00
abstract::This report will focus attention on the diagnostic and therapeutic difficulties involved in a patient who presented with a gingival papillary-verrucous lesion, which subsequently developed into a squamous cell carcinoma. ...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1987.tb01515.x
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND:There has been an explosion in research into possible associations between periodontitis and various systemic diseases and conditions. AIM:To review the evidence for associations between periodontitis and various systemic diseases and conditions, including chronic obstructive pulmonary disease (COPD), pneum...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/jcpe.12091
更新日期:2013-04-01 00:00:00
abstract:BACKGROUND AND AIM:Tobacco users and especially cigarette smokers are at higher risk than non-smokers for periodontal disease. The pathogenic mechanism has been proposed to be the vasoconstrictive properties of nicotine, with reduced gingival blood flow (GBF) as a contributing factor in the development of periodontal d...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1034/j.1600-051x.2002.290911.x
更新日期:2002-09-01 00:00:00
abstract::In a cross-sectional, epidemiological study of phenytoin induced gingival overgrowth in 77 institutionalized persons with epilepsy, the severity of the gum lesions was quantified by means of a precise new technique. Lesion severity was then compared statistically to other clinical and laboratory parameters. Positive c...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1984.tb02214.x
更新日期:1984-04-01 00:00:00
abstract::A cross-sectional study was designed to evaluate the periodontal status of 85 12-18 year-old French adolescents with insulin-dependent diabetes (IDDM) and 38 healthy controls in the same age group. The clinical examination consisted of plaque control and gingival inflammation evaluation and probing attachment level. T...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/j.1600-051x.1992.tb01710.x
更新日期:1992-10-01 00:00:00
abstract:AIM:This study evaluated the effects of 5-fluorouracil (5-FU) and cisplatin (CIS) in healthy periodontal tissues and in the early stages of experimental periodontitis (EP) in rats. METHODS:One hundred and eighty male rats were divided into three groups, which were submitted to the following systemic treatments: physio...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章
doi:10.1111/jcpe.13101
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:The aim of this Systematic Review (SR) was to assess the clinical efficacy of periodontal plastic surgery procedures in the treatment of localized gingival recessions (Rec) with or without inter-dental clinical attachment loss (iCAL). MATERIAL AND METHODS:Electronic and hand searches were performed to ident...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章,评审
doi:10.1111/jcpe.12182
更新日期:2014-04-01 00:00:00
abstract::This paper reviews some approaches to statistical analysis of dental data. Often dental data consist of more than one observation per patient which may not be independent, and consequently some standard statistical methods based on independence of all observations are inappropriate. This paper seeks to make the dental...
journal_title:Journal of clinical periodontology
pub_type: 杂志文章,评审
doi:10.1111/j.1600-051x.1990.tb01079.x
更新日期:1990-03-01 00:00:00