当前位置： SCI文献检索 > PLACENTA期刊下所有文献 > Establishment and characterization of a new human first trimester Trophoblast cell line, AL07.

Establishment and characterization of a new human first trimester Trophoblast cell line, AL07.

Abstract：

INTRODUCTION:The limited cell number of primary trophoblasts and contamination of trophoblast cell lines promote us to develop a novel stable trophoblast cell line. METHOD OF STUDY:Primary trophoblast cells were isolated from first-trimester placenta and telomerase-induced immortalization was used to immortalize these cells. Subsets of cells were then evaluated by flow cytometry using CK7, HLA-G, CD45 and CD14, specific markers for trophoblast cells, extra-villous trophoblast, pan leucocyte and monocyte/macrophage, respectively. Immunofluorescence staining and immunocytochemistry were used to detect CK7 expression in trophoblast cells. The level of secreted human Chorionic Gonadotropin (hCG) was measured by electrochemiluminescence (ECL). The Bio-Plex MAGPIX System was used to analyze the cytokines and chemokines produced by AL07 cell line. RESULTS:We were able to isolate primary trophoblast cells from several first-trimester placentas. One clone, AL07 trophoblast cells, isolated from a week 7 placenta, was morphologically stable and positive for the expression of CK7 by immunofluorescence and immunocytochemistry staining. Characterization of AL07 cells reveled that they are CD45 or CD14 negative and had constitutive secretion of hCG and low HLA-G expression. Furthermore, clone AL07 secret high levels of several cytokines and chemokines, including IL-6, IL-8 and VEGF, and moderately secreted MCP-1 IP-10 and RANTES. DISCUSSION:We report the successful isolation, immortalization and characterization of AL07 cells, a novel cell clone isolated from first trimester human placenta. The clone is free of contamination of immune cells, and exhibits similar cytokine profile as other trophoblast cell lines. This new cytotrophoblast-like AL07 cell, can be a valuable tool for in-vitro trophoblast studies in the future.

journal_name

Placenta

journal_title

Placenta

authors

Liu H,Wang L,Wang Y,Zhu Q,Aldo P,Ding J,Mor G,Liao A

doi

10.1016/j.placenta.2020.08.013

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

122-132

eissn

0143-4004

issn

1532-3102

pii

S0143-4004(20)30266-6

journal_volume

100

pub_type

杂志文章

相关文献

PLACENTA文献大全
  • Comparing human and macaque placental transcriptomes to disentangle preterm birth pathology from gestational age effects.

    abstract：INTRODUCTION:A major issue in the transcriptomic study of spontaneous preterm birth (sPTB) in humans is the inability to collect healthy control tissue at the same gestational age (GA) to compare with pathologic preterm tissue. Thus, gene expression differences identified after the standard comparison of sPTB and term ...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2016.03.006

    authors： Eidem HR,Rinker DC,Ackerman WE 4th,Buhimschi IA,Buhimschi CS,Dunn-Fletcher C,Kallapur SG,Pavličev M,Muglia LJ,Abbot P,Rokas A

    更新日期：2016-05-01 00:00:00

  • DNA content and ploidy level of bovine placentomal trophoblast giant cells.

    abstract：:Cytophotometric measurement of the DNA content of Feulgen-stained nuclei in touch preparations of bovine placentomes (n =5) revealed that 8C nuclei occurred in all, 16C nuclei in two, and 32C nuclei in one specimen. The determination of ploidy level by in situ hybridization with a Y-chromosome specific DNA probe showe...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1053/plac.1999.0402

    authors： Klisch K,Hecht W,Pfarrer C,Schuler G,Hoffmann B,Leiser R

    更新日期：1999-07-01 00:00:00

  • Apoptotic activity in villous trophoblast cells during B19 infection correlates with clinical outcome: assessment by the caspase-related M30 Cytodeath antibody.

    abstract：:Human parvovirus B19 (B19) infection during pregnancy can result in horizontal transmission of the virus and congenital infection. The main targets for B19 replication are the erythroid precursor cell of the colony and burst forming units. The cellular receptor necessary for B19 infectivity is globoside. Other non-ery...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1053/plac.2002.0843

    authors： Jordan JA,Butchko AR

    更新日期：2002-08-01 00:00:00

  • Distensible transtrophoblastic channels in the rat placenta.

    abstract：:Paracellular pathways in the haemotrichorial placenta of the rat were studied by electron microscopy using lanthanum hydroxide as an electron dense marker. Near term placentae were dually perfused in situ, adding lanthanum to the fetal perfusate. In some placentae outflow pressure on the fetal side was elevated (betwe...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1053/plac.2000.0558

    authors： Kertschanska S,Stulcová B,Kaufmann P,Stulc J

    更新日期：2000-09-01 00:00:00

  • What can comparative studies of placental structure tell us?--A review.

    abstract：:The diversity of placental structures in Eutherian mammals is such that drawing generalizations from the definitive forms is problematic. There are always areas of reduced interhaemal distance whether the placenta is epitheliochorial, synepitheliochorial, endotheliochorial or haemochorial. However, the thinning may be...

    journal_title：Placenta

    pub_type： 杂志文章,评审

    doi：10.1016/j.placenta.2004.01.011

    authors： Enders AC,Carter AM

    更新日期：2004-04-01 00:00:00

  • A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue.

    abstract：:A rapid method to determine quality for placental proteomic studies is required due to varying lengths of time between delivery and sampling in routine protocols. We developed a rapid 10 min LC-MS based scanning method to profile free peptides liberated from natural proteolytic degradation. The assay was applied to pl...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2016.04.014

    authors： Heywood WE,Pryce J,Virasami A,Preece RL,Dezateux C,Mills K,Sebire NJ

    更新日期：2016-08-01 00:00:00

  • Genomic imprinting and mammalian development.

    abstract：:Genomic imprinting, which results in the mono-allelic expression of certain genes in a parent of origin-dependent manner, represents a specialized form of gene regulation which may be vitally important for mammalian development. The mechanisms which underlie imprinting and the molecular nature of the imprint itself re...

    journal_title：Placenta

    pub_type： 杂志文章,评审

    doi：10.1016/s0143-4004(05)80638-1

    authors： Franklin GC,Adam GI,Ohlsson R

    更新日期：1996-01-01 00:00:00

  • Cryopreservation of placental biopsies for mitochondrial respiratory analysis.

    abstract：:Mitochondrial function is required to support energetically-demanding processes in the placenta. As such, a compromise in mitochondrial function could severely impact fetal growth and development. Respirometry is a highly useful method for studying mitochondrial function, but is not possible in freeze-thawed mitochond...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2011.11.019

    authors： Colleoni F,Morash AJ,Ashmore T,Monk M,Burton GJ,Murray AJ

    更新日期：2012-02-01 00:00:00

  • IFPA meeting 2008 workshops report.

    abstract：:Workshops are an important part of the IFPA annual meeting. At the IFPA meeting 2008 diverse topics were discussed in 12 themed workshops. Topics covered included: immunology of placentation; galectins and trophoblast invasion; signaling in implantation and invasion; markers to identify trophoblast subpopulations; pla...

    journal_title：Placenta

    pub_type：

    doi：10.1016/j.placenta.2008.11.014

    authors： Lash GE,Ansari T,Bischof P,Burton GJ,Chamley L,Crocker I,Dantzer V,Desoye G,Drewlo S,Fazleabas A,Jansson T,Keating S,Kliman HJ,Lang I,Mayhew T,Meiri H,Miller RK,Nelson DM,Pfarrer C,Roberts C,Sammar M,Sharma S,

    更新日期：2009-03-01 00:00:00

  • Decreased mitochondrial fatty acid oxidation in placentas from women with preeclampsia.

    abstract：:Preeclampsia is a leading cause of maternal and fetal morbidity and mortality in high and low-income countries. The aetiology of preeclampsia is multifactorial and remains obscure. Some evidences suggest that altered placental fatty acid oxidation might play a role in the pathogenesis of preeclampsia. To reveal if pla...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2011.11.027

    authors： Bartha JL,Visiedo F,Fernández-Deudero A,Bugatto F,Perdomo G

    更新日期：2012-02-01 00:00:00

  • Mononuclear phagocytes in the human placenta.

    abstract：:Hofbauer cells have been identified by morphological criteria in human placentae and are considered to be mononuclear phagocytes. An increasing recognition of the morphological heterogeneity of mononuclear phagocytes and of the considerable difficulty in their recognition in histological sections has suggested that th...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/s0143-4004(80)80019-1

    authors： Wood GW

    更新日期：1980-04-01 00:00:00

  • Expression of thioredoxin and thioredoxin reductase in placentae of pregnant mice exposed to lipopolysaccharide.

    abstract：:We have previously shown that thioredoxin and thioredoxin reductase were immunohistochemically localized in cytotrophoblasts, decidua and stromal cells in the stem villi of human placenta and that the addition of exogenous thioredoxin and thioredoxin reductase to mitochondrial fractions from human placenta displayed a...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1053/plac.1999.0415

    authors： Ejima K,Koji T,Nanri H,Kashimura M,Ikeda M

    更新日期：1999-09-01 00:00:00

  • Trophoblast debris extruded from preeclamptic placentae activates endothelial cells: a mechanism by which the placenta communicates with the maternal endothelium.

    abstract：INTRODUCTION:Preeclampsia is characterized by maternal endothelial dysfunction. While the mechanisms leading to preeclampsia are unclear, a factor(s) from the placenta is responsible for triggering the disease. One placental factor implicated in triggering preeclampsia is trophoblast debris which may transmit pathogeni...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2014.07.009

    authors： Shen F,Wei J,Snowise S,DeSousa J,Stone P,Viall C,Chen Q,Chamley L

    更新日期：2014-10-01 00:00:00

  • Nitrotyrosine immunostaining correlates with increased extracellular matrix: evidence of postplacental hypoxia.

    abstract：:Nitrotyrosine residues (NT), an index of oxidative stress arising from peroxynitrite formation and action, are found in placental vasculature of pregnancies complicated by pre-eclampsia (PE) or pregestational insulin-dependent diabetes mellitus (IDDM). This study correlates conventional placental pathology with NT imm...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1053/plac.2001.0634

    authors： Stanek J,Eis AL,Myatt L

    更新日期：2001-04-01 00:00:00

  • Villous trophoblast apoptosis is elevated and restricted to cytotrophoblasts in pregnancies complicated by preeclampsia, IUGR, or preeclampsia with IUGR.

    abstract：:Human placental villi are surfaced by an outer multinucleated syncytiotrophoblast and underlying mononucleated cytotrophoblasts. Conflicting data have attributed one, or the other, of these villous trophoblast phenotypes to undergo enhanced apoptosis in complicated pregnancies, compared to term, normotensive pregnanci...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2012.01.017

    authors： Longtine MS,Chen B,Odibo AO,Zhong Y,Nelson DM

    更新日期：2012-05-01 00:00:00

  • Secretory phospholipases and membrane polishing.

    abstract：:Although secretory phospholipase A2 (PLA2) isozymes have been identified in human gestational tissues, their role in homeostasis and pathophysiology during pregnancy has yet to be clearly established. The aims of this brief commentary are: (1) to review recent data concerning the expression of secretory PLA2 isozymes ...

    journal_title：Placenta

    pub_type： 杂志文章,评审

    doi：10.1016/s0143-4004(98)90093-5

    authors： Rice GE

    更新日期：1998-01-01 00:00:00

  • Comparative studies of mesenchymal stem cells derived from different cord tissue compartments - The influence of cryopreservation and growth media.

    abstract：INTRODUCTION:We have identified some critical aspects concerning umbilical cord tissue mesenchymal stem cells: the lack of standards for cell isolation, expansion and cryopreservation, the lack of unanimous opinions upon their multilineage differentiation potential and the existence of very few results related to the f...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2015.08.011

    authors： Dulugiac M,Moldovan L,Zarnescu O

    更新日期：2015-10-01 00:00:00

  • Upregulated ARRDC3 limits trophoblast cell invasion and tube formation and is associated with preeclampsia.

    abstract：OBJECTIVE:Bioinformatics analysis indicated that the arrestin ARRDC3 was upregulated in placental tissue from patients with preeclampsia (PE). The study aimed to confirm the finding by examining placenta samples from women with and without early-onset PE and to investigate ARRDC3 roles in trophoblast function. METHODS...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2019.10.009

    authors： Lei D,Deng N,Wang S,Huang J,Fan C

    更新日期：2020-01-01 00:00:00

  • Sequential expression of VEGF and its receptors in human placental villi during very early pregnancy: differences between placental vasculogenesis and angiogenesis.

    abstract：:Vascularization within the human placenta is the result of the de novo formation of vessels derived from pluripotent precursor cells in the mesenchymal core of the villi. Vascularization of placental villi starts at around day 21 post conception (p.c.) with a four somite embryo. At this stage progenitors of haemangiog...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2003.11.011

    authors： Demir R,Kayisli UA,Seval Y,Celik-Ozenci C,Korgun ET,Demir-Weusten AY,Huppertz B

    更新日期：2004-07-01 00:00:00

  • Redness discordance in monochorionic twin placentas: Correlation with clinical and placental findings.

    abstract：INTRODUCTION/OBJECTIVES:Recent studies suggest redness (color) discordance of the placental basal plate may be a marker for twin anemia-polycythemia sequence (TAPS), a recently described complication of diamniotic-monochorionic twinning characterized by marked intertwin hemoglobin (Hb) discordance in the absence of oli...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2017.10.007

    authors： De Paepe ME,Gündoğan F,Mao Q,Chu S,Shapiro S

    更新日期：2017-12-01 00:00:00

  • Expression and distribution of tight junction proteins in human amnion during late pregnancy.

    abstract：:Amnion is the innermost layer of the fetal membrane and has been suggested to regulate the volume of amniotic fluid via the amniotic epithelium. The transepithelial pathway is generally restricted by tight junctions (TJs). Thus far, human amniotic TJs have not been identified. In this study, we determined whether the ...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2009.11.014

    authors： Kobayashi K,Kadohira I,Tanaka M,Yoshimura Y,Ikeda K,Yasui M

    更新日期：2010-02-01 00:00:00

  • Immunohistochemical detection of human placental cytochrome P-450-associated mono-oxygenase system inducible by maternal cigarette smoking.

    abstract：:Several xenobiotic-metabolizing mono-oxygenase activities, in conjunction with the immunohistochemical localization of the respective cytochrome P-450 forms, were investigated in placentae from smoking and non-smoking women. The antibodies used in the immunohistochemical analyses were monoclonal antibodies (MAbs) 1-7-...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/0143-4004(88)90034-3

    authors： Pasanen M,Stenbäck F,Park SS,Gelboin HV,Pelkonen O

    更新日期：1988-05-01 00:00:00

  • Regulation of placental efficiency for nutrient transport by imprinted genes.

    abstract：:Intrauterine growth and development can impact upon the long-term health of an individual. The fetus is dependent upon the placenta for its supply of nutrients and oxygen from the mother. In turn, the functional capacity of the placenta to supply that demand is under the control of the fetal and maternal genomes. Rece...

    journal_title：Placenta

    pub_type： 杂志文章,评审

    doi：10.1016/j.placenta.2005.12.008

    authors： Angiolini E,Fowden A,Coan P,Sandovici I,Smith P,Dean W,Burton G,Tycko B,Reik W,Sibley C,Constância M

    更新日期：2006-04-01 00:00:00

  • Patterns of expression of Bax, Bcl-2 and Bcl-x(L) in the implantation site in rat during pregnancy.

    abstract：:The uterine endometrium responds to blastocyst implantation with extensive proliferation and differentiation of stromal cells into decidual cells, forming the antimesometrial and mesometrial decidua. These undergo regression by apoptosis but as this process occurs at different time periods suggest that there is spatia...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2004.09.008

    authors： Correia-da-Silva G,Bell SC,Pringle JH,Teixeira NA

    更新日期：2005-11-01 00:00:00

  • Advanced oxidation protein products enhances soluble Fms-like tyrosine kinase 1 expression in trophoblasts: a possible link between oxidative stress and preeclampsia.

    abstract：:Accumulation of advanced oxidation protein products (AOPPs) is prevalent in obesity, advanced maternal age, diabetes mellitus, and polycystic ovary syndrome. Alterations in the regulation and signaling of angiogenic pathways have been recognized as a link between these conditions and pre-eclampsia. To investigate the ...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2013.06.308

    authors： Huang QT,Wang SS,Zhang M,Huang LP,Tian JW,Yu YH,Wang ZJ,Zhong M

    更新日期：2013-10-01 00:00:00

  • Potential clinical applications of placental stem cells for use in fetal therapy of birth defects.

    abstract：:Placental stem cells are of growing interest for a variety of clinical applications due to their multipotency and ready availability from otherwise frequently discarded biomaterial. Stem cells derived from the placenta have been investigated in a number of disease processes, including wound healing, ischemic heart dis...

    journal_title：Placenta

    pub_type： 杂志文章,评审

    doi：10.1016/j.placenta.2017.05.010

    authors： Vanover M,Wang A,Farmer D

    更新日期：2017-11-01 00:00:00

  • 2005 Trophoblast Research Award Lecture: Defects in the keratin cytoskeleton disrupt normal murine placental development and trophoblast cell function.

    abstract：:The keratin cytoskeleton is present in all trophoblast cell subtypes of the mouse and human placenta and is required to maintain the structural integrity of these cells. Recently, various genetic mouse models have shown that a normal keratin network is necessary for placental development. Keratin-deficiency leads to t...

    journal_title：Placenta

    pub_type：

    doi：10.1016/j.placenta.2007.01.006

    authors： Watson ED

    更新日期：2007-04-01 00:00:00

  • Placental magnetic resonance imaging in chronic hypertension: A case-control study.

    abstract：INTRODUCTION:We aimed to explore the use of magnetic resonance imaging (MRI) in vivo as a tool to elucidate the placental phenotype in women with chronic hypertension. METHODS:In case-control study, women with chronic hypertension and those with uncomplicated pregnancies were imaged using either a 3T Achieva or 1.5T I...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2020.12.006

    authors： Ho A,Hutter J,Slator P,Jackson L,Seed PT,Mccabe L,Al-Adnani M,Marnerides A,George S,Story L,Hajnal JV,Rutherford M,Chappell LC

    更新日期：2020-12-13 00:00:00

  • Non-selective cationic channels (NSCC) in smooth muscle cells from human umbilical arteries.

    abstract：:The aim of our work was to investigate the presence of non-selective cation channels (NSCC) in freshly isolated smooth muscle cells from the human umbilical artery (HUA), one of the vessels involved in fetal-placental circulation. We studied the electrophysiological properties of NSCC using the patch-clamp technique i...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2004.01.028

    authors： Raingo J,Rebolledo A,Iveli F,Grassi de Gende AO,Milesi V

    更新日期：2004-09-01 00:00:00

  • Three-dimensional arrangement of the capillary bed and its relationship to microrheology in the terminal villi of normal term placenta.

    abstract：:Spatial arrangement of the capillary bed, manifestations of its growth and symmetry of capillary branching were studied in peripheral villi of normal human placenta at term using confocal microscopy and image analysis. Unlike the model that has been accepted so far, it was shown that the arrangement of the capillary b...

    journal_title：Placenta

    pub_type： 杂志文章

    doi：10.1016/j.placenta.2008.07.004

    authors： Jirkovská M,Janácek J,Kaláb J,Kubínová L

    更新日期：2008-10-01 00:00:00