Abstract:
:A rapid method to determine quality for placental proteomic studies is required due to varying lengths of time between delivery and sampling in routine protocols. We developed a rapid 10 min LC-MS based scanning method to profile free peptides liberated from natural proteolytic degradation. The assay was applied to placenta samples obtained following refrigeration for varying time periods post-delivery (12 h, +24 h, +48 h and +72 h). Analysis reveals time dependant overlapping profiles for groups <24 to +48 h with greatest variation in the +72 h group, indicating that significant proteolysis affects tissue integrity between 48 and 72 h.
journal_name
Placentajournal_title
Placentaauthors
Heywood WE,Pryce J,Virasami A,Preece RL,Dezateux C,Mills K,Sebire NJdoi
10.1016/j.placenta.2016.04.014subject
Has Abstractpub_date
2016-08-01 00:00:00pages
109-11eissn
0143-4004issn
1532-3102pii
S0143-4004(16)30065-0journal_volume
44pub_type
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