Abstract:
:When cultured in keratinocyte growth medium, mononuclear cytotrophoblast cells aggregate into multicellular colonies which then fuse to form multinucleated syncytiotrophoblast. In an attempt to characterize better the mechanism of human trophoblast differentiation and to obtain information about the role of the cytoskeleton, experiments were performed using cytoskeletal-disrupting drugs and primary cultures of cytotrophoblast cells from term placentae. Addition of colchicine 6 h after plating permitted aggregation but the cells did not form syncytiotrophoblast, as revealed by staining for desmosomes and nuclei. Staining with an anti-tubulin antibody showed that microtubules were present in untreated control cells but absent in colchicine-treated cultures. If colchicine was added 24 h after plating, the cells also failed to differentiate. When cells were exposed to colchicine for the first 24 h after plating and then cultured in the absence of the drug, differentiation proceeded normally. Cells exposed to colchicine for 48 h and then incubated in the absence of the drug failed to form syncytiotrophoblast. The results suggest that a decision to differentiate is made between 24 and 48 h after plating. The effects of colchicine were observed between 2.5 and 250 microM. Beta-lumicolchicine blocked differentiation at 250 microM but was ineffective at lower concentrations. Colchicine also inhibited HCG secretion in a dose-dependent manner; beta-lumicolchicine only caused inhibition at 250 microM. Staining with antitubulin antibody revealed that lumicolchicine-treated cells had intact microtubules. These results suggest a role for microtubules in trophoblast differentiation.
journal_name
Placentajournal_title
Placentaauthors
Douglas GC,King BFdoi
10.1016/s0143-4004(05)80260-7subject
Has Abstractpub_date
1993-03-01 00:00:00pages
187-201issue
2eissn
0143-4004issn
1532-3102pii
S0143-4004(05)80260-7journal_volume
14pub_type
杂志文章相关文献
PLACENTA文献大全abstract::In the present study, we characterized the expression of lymphocyte antigen 6, locus E (Ly6e) in mouse placental trophoblast. We identified Ly6e mRNA expression in trophoblast stem (TS) cells by a gene expression screen. In vivo, Ly6e was first detectable by mRNA in situ hybridization in the chorion beginning at E8.5 ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2013.05.011
更新日期:2013-09-01 00:00:00
abstract::During pregnancy, leptin concentrations in the maternal circulation are elevated in both humans and rodents but decrease to pre-pregnancy levels at birth, suggesting a role for leptin in the maintenance of pregnancy. Synthesis of leptin by the human placenta is established but whether the murine placenta synthesizes l...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2004.03.009
更新日期:2005-01-01 00:00:00
abstract::We have investigated whether maternal peripheral blood from the first trimester of pregnancy is a reliable source of identifiable trophoblast cells. The cells were enriched from 30 ml of venous blood, with multiple antibodies shown previously to enrich trophoblasts and a new cocktail based on known trophoblast surface...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1053/plac.2001.0704
更新日期:2001-09-01 00:00:00
abstract::It will be apparent from this review that the exact role of IFN on human placental development remains largely undefined. However, there is now sufficient data available on the biological functions of this cytokine in other cellular systems to provide a conceptual framework upon which the influence of IFN on the human...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/s0143-4004(05)80220-6
更新日期:1990-07-01 00:00:00
abstract:INTRODUCTION:Feeding pregnant broodmares with cereal concentrates has been shown to increase maternal insulin resistance and affect foal metabolism in the short and long-term. These effects are likely to be mediated by the placenta. Here, we investigated feto-placental biometry and placental structure and function at t...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2018.02.003
更新日期:2018-04-01 00:00:00
abstract::This study examines middle and late gestational placentae from 13 Tayassu tajacu (collared peccary) and 3 Tayassu pecari (white-lipped peccary), which are Artiodactyla belonging to the Family Tayassuidae. The chorionic sac of Tayassu species is diffuse and chorioallantoic. These epitheliochorial placentae show no trop...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2005.02.017
更新日期:2006-02-01 00:00:00
abstract:INTRODUCTION:Chronic villitis of unknown etiology (CVUE) and massive chronic intervillositis (MCI) are placental lesions associated with infiltration of mononuclear cells in the chorionic villi and the intervillous spaces, respectively. It is not well known whether immune cells in CVUE and MCI have similar phenotypic c...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2015.03.008
更新日期:2015-06-01 00:00:00
abstract::The aim of our work was to investigate the presence of non-selective cation channels (NSCC) in freshly isolated smooth muscle cells from the human umbilical artery (HUA), one of the vessels involved in fetal-placental circulation. We studied the electrophysiological properties of NSCC using the patch-clamp technique i...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2004.01.028
更新日期:2004-09-01 00:00:00
abstract::In primates and rodents, trophoblast cells synthesize and secrete into the maternal circulation a family of proteins known as pregnancy specific glycoproteins (PSG). The current study was undertaken to characterize the receptor for two members of the murine PSG family, PSG17 and PSG23. Binding of recombinant PSG17 and...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2011.05.008
更新日期:2011-08-01 00:00:00
abstract:INTRODUCTION:Little is known about the interaction between human placental multipotent mesenchymal stromal cell (hPMSC) and trophoblast. We hypothesize that hPMSCs produce hepatocyte growth factor (HGF) which may interact with trophoblasts and regulate their migration during placentation. METHODS:hPMSCs were isolated ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2013.06.311
更新日期:2013-10-01 00:00:00
abstract:OBJECTIVE:Pregestational maternal obesity (PGMO) associates with foetoplacental vascular endothelial dysfunction and higher risk for insulin resistance in the neonate. We characterised the PGMO consequences on the insulin response of the human foetoplacental vasculature. METHODS:Umbilical veins were from pregnancies w...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2019.07.007
更新日期:2019-10-01 00:00:00
abstract::There is inconsistent use of Matrigel for experiments with the HTR8/SVneo first trimester trophoblast and other cell lines. We quantified the effects of Matrigel on the expression of genes considered to be markers of extravillous cytotrophoblast (EVT) differentiation and invasive potential. Culture on Matrigel promote...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2012.04.003
更新日期:2012-07-01 00:00:00
abstract::The human placenta in vivo consumes large amounts of oxygen. After delivery the placenta is exposed to anoxia and under in vitro experimental conditions oxygen consumption is only a fraction of in vivo estimates. In spite of a reduced oxygen supply, structural and functional integrity of the tissue is surprisingly wel...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1053/plac.1999.0512
更新日期:2000-03-01 00:00:00
abstract:INTRODUCTION:In the placenta, the (pro)renin receptor (ATP6AP2) is localised to the syncytiotrophoblast. ATP6AP2 can activate the placental renin-angiotensin system (RAS), producing Angiotensin II (Ang II) which, acting via the angiotensin II type 1 receptor (AGTR1), is important for placental development and function....
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2020.05.009
更新日期:2020-08-01 00:00:00
abstract::Recent demonstrations of transferrin (TF) mRNA in placental tissue raised the possibility that the placenta may serve as an extra-hepatic source of this iron-binding protein during development. In this study, we first confirmed these findings using cRNA probes for TF, and then adapted the TF reverse haemolytic plaque ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(05)80419-9
更新日期:1994-07-01 00:00:00
abstract:INTRODUCTION:Knockout mouse model has shown a relationship between Slit2/Robo1 signalling and altered fertility. Altered expression by endometrial epithelium and trophoblast and is associated with the pathogenesis of pregnancy complications but few studies have investigated the expression of decidual Slit2 in miscarria...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2020.10.008
更新日期:2020-10-09 00:00:00
abstract::Placental ultrasonographic, bio- and histochemical studies were performed on four unrelated fetuses affected with Niemann-Pick disease Type A, following prostaglandin-induced abortion at about the 19th week of gestation. An accumulation of sphingomyelin in the placentae of affected fetuses indicates the essential role...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(85)80030-8
更新日期:1985-01-01 00:00:00
abstract::Placental development is a highly regulated process requiring signals from both fetal and maternal uterine compartments. Within this complex system, trophoblasts, placental cells of epithelial lineage, form the maternal-fetal interface controlling nutrient, gas and waste exchange. The commitment of progenitor villous ...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/j.placenta.2014.12.016
更新日期:2015-04-01 00:00:00
abstract::Bovine nuclear transfer pregnancies are characterized by a high incidence of placental abnormalities, notably, increased placentome size and deficiencies in trophoblast cell function and establishment of placental vasculature. Alterations in gene expression during placental growth and development may contribute to the...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2005.03.002
更新日期:2006-02-01 00:00:00
abstract::The ability of three streptococcal isolates of different pathogenicity to multiply in the tissues of pregnant mice was investigated following intravenous injection. The highly pathogenic isolate multiplied most rapidly whilst the isolate of low pathogenicity showed the least rapid growth within the observation period ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(81)80001-x
更新日期:1981-07-01 00:00:00
abstract::A protein immunologically identical to the major basic protein (MBP) of eosinophil granules is present in the plasma of pregnant women and has been localized to human placental X cells using immunofluorescence. This study demonstrates the presence of similar immunoreactive molecules in the placental X cells from sever...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/0143-4004(87)90052-x
更新日期:1987-05-01 00:00:00
abstract::The fetus has a substantial demand for glycine, which is satisfied in part by placental formation. The ability to form glycine through the activity of alanine:glyoxylate aminotransferase enzyme was measured in placentae from normal term human pregnancies and placentae from rats at day 20 of gestation. There was no det...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(98)90066-2
更新日期:1998-05-01 00:00:00
abstract::Pregnancy-associated plasma protein-A was assayed in the blood of 347 women during pregnancy, using a new primary standard of PAPP-A as reference. The protein was assayed by antibody-antigen crossed electrophoresis with the lower limit of confident assay being 9.5 micrograms PAPP-A/ml (13 pmol/ml). PAPP-A was first de...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(82)80020-9
更新日期:1982-01-01 00:00:00
abstract::11β-Hydroxysteroid dehydrogenase 1 and 2 (11β-HSD1 and 11β-HSD2) are involved in the complex mechanism of human parturition. The present study examined mRNA expression and activity of membrane 11β-HSD1 and placental 11β-HSD2 in postdate pregnancies according to response of labor induction. In comparison to postdate wo...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2013.08.011
更新日期:2013-11-01 00:00:00
abstract::The implantation of the blastocyst and early development of the placenta are crucial for the success of implantation and pregnancy. However, the formative stages of human placental development are largely unknown because of their existence in a 'black box' where access to samples is extremely limited for ethical reaso...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/j.placenta.2012.01.020
更新日期:2012-05-01 00:00:00
abstract::Confocal and conventional indirect immunofluorescence and immunogold electron microscopic methods were applied to examine the distribution of extracellular matrix constituents (collagens types III and IV) in the villi of immature and term human placentae. The immunofluorescence study revealed that collagen type III is...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/0143-4004(91)90493-y
更新日期:1991-11-01 00:00:00
abstract:INTRODUCTION:Perfluorinated alkyl acids (PFAAs) are widely used in industry and consumer products. Pregnant women are exposed to PFAAs and their presence in umbilical cord blood represents fetal exposure. Interestingly, PFAAs are substrates for organic anion transporters (OAT) of which OAT4 is expressed in human placen...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2015.07.119
更新日期:2015-10-01 00:00:00
abstract::Human placental fragments concentrate 86Rb 10--20-fold during a two-hour incubation period. Inhibition of ouabain is dose-dependent, reaching 90 + per cent at a concentration of 5 x 10(-5) M. The clearance index of 86Rb across the perfused human placenta is 0.34 +/- 0.08, comparing to previously reported indices for N...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(83)80038-1
更新日期:1983-10-01 00:00:00
abstract::This report compares the relative levels of messenger RNA species coding for plasma proteins in rat visceral yolk sac and fetal liver from 12.5 days to 21.5 days gestation. Transthyretin, retinol-binding protein, transferrin and alpha 1-fetoprotein mRNAs were detected in both tissues, although relative levels were muc...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(05)80216-4
更新日期:1990-09-01 00:00:00
abstract:INTRODUCTION:Placental thickness in the second trimester of pregnancy has been associated with risks of placenta-mediated complications of pregnancy. We aimed to estimate the association between first-trimester maximum placental thickness and the subsequent risk of preeclampsia and/or the delivery of small-for-gestatio...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2017.06.016
更新日期:2017-09-01 00:00:00