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ELKS1 Captures Rab6-Marked Vesicular Cargo in Presynaptic Nerve Terminals.

Abstract：

:Neurons face unique transport challenges. They need to deliver cargo over long axonal distances and to many presynaptic nerve terminals. Rab GTPases are master regulators of vesicular traffic, but essential presynaptic Rabs have not been identified. Here, we find that Rab6, a Golgi-derived GTPase for constitutive secretion, associates with mobile axonal cargo and localizes to nerve terminals. ELKS1 is a stationary presynaptic protein with Golgin homology that binds to Rab6. Knockout and rescue experiments for ELKS1 and Rab6 establish that ELKS1 captures Rab6 cargo. The ELKS1-Rab6-capturing mechanism can be transferred to mitochondria by mistargeting ELKS1 or Rab6 to them. We conclude that nerve terminals have repurposed mechanisms from constitutive exocytosis for their highly regulated secretion. By employing Golgin-like mechanisms with anchored ELKS extending its coiled-coils to capture Rab6 cargo, they have spatially separated cargo capture from fusion. ELKS complexes connect to active zones and may mediate vesicle progression toward release sites.

journal_name

Cell Rep

journal_title

Cell reports

authors

Nyitrai H,Wang SSH,Kaeser PS

doi

10.1016/j.celrep.2020.107712

subject

Has Abstract

pub_date

2020-06-09 00:00:00

pages

107712

issue

10

issn

2211-1247

pii

S2211-1247(20)30689-6

journal_volume

31

pub_type

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