当前位置： SCI文献检索 > DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY期刊下所有文献 > The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis.

The involvement of ecdysone and ecdysone receptor in regulating the expression of antimicrobial peptides in Chinese mitten crab, Eriocheir sinensis.

Abstract：

:The ecdysone, 20-hydroxyecdysone (20E) and ecdysone receptor (EcR), are regarded as the key regulators of development, metamorphosis, and growth in arthropods. In the present study, the role of 20E and EsEcR in regulating the expression of antimicrobial peptides (AMPs) was investigated in Chinese mitten crab, Eriocheir sinensis. The concentration of 20E in plasma was significantly (p < 0.05) up-regulated from 3 h to 12 h after lipopolysaccharide (LPS) stimulation. The mRNA expression level of EsEcR-4 in hemocytes was significantly (p < 0.01) up-regulated from 6 h to 24 h after LPS stimulation, while no significant changes of EsEcR-2 and EsEcR-3 transcripts were observed. After 20E injection, EsEcR-4 expression level was significantly increased from 12 h to 48 h with the highest level at 24 h (4.34-fold compared to the control group, p < 0.01), and the mRNA expression levels of AMPs (EsALF-2, EsLYZ and EsCrus) in hemocytes were significantly increased from 6 h to 24 h with the peak level of 2.93-fold (p < 0.01), 2.33-fold (p < 0.01) and 2.75-fold (p < 0.01) at 12 h, respectively. After EsEcR-4 expression was interfered with specific dsRNA, a significant reduction of EsALF-2 (0.56-fold compared to the control group, p < 0.01), EsLYZ (0.27-fold, p < 0.01) and EsCrus (0.41-fold, p < 0.01) mRNA expression level was observed in dsEsEcR-4+LPS group at 12 h post LPS stimulation. Moreover, the mRNA expression levels of EsDorsal and EsJNK in hemocytes were significantly (p < 0.05) increased from 6 h to 24 h post 20E injection, and the phosphorylation of Dorsal and JNK in the hemocytes were significantly (p < 0.01) up-regulated at 3 h post 20E injection, while that in dsEsEcR-4+LPS group were significantly decreased after LPS stimulation compared to dsEsEGFP+LPS group. Taken together, these results suggested that 20E and EsEcR-4 play important roles in regulating the expression level of AMPs in the immune responses of E. sinensis by regulating the mRNA expression level and phosphorylation of Dorsal and JNK.

journal_name

Dev Comp Immunol

journal_title

Developmental and comparative immunology

authors

Huang S,Yi Q,Lian X,Xu S,Yang C,Sun J,Wang L,Song L

doi

10.1016/j.dci.2020.103757

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

103757

eissn

0145-305X

issn

1879-0089

pii

S0145-305X(20)30099-9

journal_volume

111

pub_type

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