Abstract:
:This study compared bovine serum albumin (BSA) adsorption onto octacalcium phosphate (OCP) materials prepared from two wet preparations in the absence (w-OCP) and presence (c-OCP) of gelatin. Raman spectroscopy was used to analyze the BSA adsorption onto OCPs in a 150 mM Tris-HCl buffer containing 0.5 mM calcium and inorganic phosphate (Pi) ions at pH 7.4 and at 37°C. The degree of supersaturation of the supernatants after the adsorption was determined by measuring the ion composition. The results showed that BSA adsorption onto w-OCP was higher than that for c-OCP. The calcium ion concentration of the supernatant decreased for both w-OCP and c-OCP, whereas the Pi ion concentration increased, approaching OCP equilibria at different saturation levels. BSA adsorbed even onto c-OCP, which included a small amount of gelatin during c-OCP preparation. These results indicate that the biodegradability of w-OCP and c-OCP may be modulated through interactions with serum proteins.
journal_name
Dent Mater Jjournal_title
Dental materials journalauthors
Tsuchiya K,Hamai R,Sakai S,Suzuki Odoi
10.4012/dmj.2019-250subject
Has Abstractpub_date
2020-09-29 00:00:00pages
883-891issue
5eissn
0287-4547issn
1881-1361journal_volume
39pub_type
杂志文章abstract::The aim of this study was to evaluate the cytotoxicity of contemporary flowable and paste-like bulk-fill dental composites by using a real-time cell analysis. In the present paper, cytotoxicity levels of five flowable, five paste-like bulk-fill composite materials and one conventional flowable, one conventional paste-...
journal_title:Dental materials journal
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journal_title:Dental materials journal
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