Identification of peptide motif that binds to the surface of zirconia.

Abstract:

:A zirconia-binding peptide motif was identified using a peptide phage display system. Yttria stabilized zirconia beads and discs were used as the target. Quartz crystal microbalance was used to monitor the binding of phages to zirconia. Starting from a library of phages displaying random sequences of 12-mer peptides, we repeated cycles of biopanning against zirconia beads. After four cycles of biopanning, we isolated a phage clone Φ#17. DNA sequencing of the corresponding portion of Φ#17 unexpectedly revealed that it displayed a 58-mer peptide (amino acid sequence: WMPSDVDINDPQGGGSRPNLHQPKPAAEAASKKKSENRKVPFYSHSWY-SSMSEDKRGW). We found that Φ#17 had a 300-fold, significantly higher binding affinity for zirconia discs than phages displaying no peptide. In quartz crystal microbalance assay, a rapid increase in energy dissipation was observed from Φ#17 but not from the control phages, indicating that Φ#17 binds to the surface of zirconia via its displayed peptide. We successfully identified a peptide motif that binds zirconia.

journal_name

Dent Mater J

journal_title

Dental materials journal

authors

Hashimoto K,Yoshinari M,Matsuzaka K,Shiba K,Inoue T

doi

10.4012/dmj.2011-161

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

935-40

issue

6

eissn

0287-4547

issn

1881-1361

pii

JST.JSTAGE/dmj/2011-161

journal_volume

30

pub_type

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