Abstract：
:The hepatitis B virus (HBV) is a major cause of hepatocellular carcinoma (HCC), partly driven by viral integration and specific oncogenic HBV variants. However, the biological significance of HBV genomes within lymphoid cells (i.e., peripheral blood mononuclear cells, PBMCs) is unclear. Here, we collected available plasma, PBMC, liver, and tumor from 52 chronic HBV (CHB) carriers: 32 with HCC, 19 without HCC, and one with dendritic cell sarcoma, DCS. Using highly sensitive sequencing techniques, next generation sequencing, and AluPCR, we demonstrate that viral genomes (i.e., HBV DNA, RNA, and cccDNA), oncogenic variants, and HBV-host integration are often found in all sample types collected from 52 patients (including lymphoid cells and a DCS tumor). Viral integration was recurrently identified (n = 90 such hits) in genes associated with oncogenic consequences in lymphoid and liver cells. Further, HBV genomes increased in PBMCs derived from 7 additional (treated or untreated) CHB carriers after extracellular mitogen stimulation. Our study shows novel HBV molecular data and replication not only liver, but also within 63.8% of lymphoid cells analysed (including a representative lymphoid cell malignancy), that was enhanced in ex vivo stimulated PBMC.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Lau KC,Joshi SS,Gao S,Giles E,Swidinsky K,van Marle G,Bathe OF,Urbanski SJ,Terrault NA,Burak KW,Osiowy C,Coffin CSdoi
10.1016/j.canlet.2020.03.022subject
Has Abstractpub_date
2020-06-28 00:00:00pages
39-47eissn
0304-3835issn
1872-7980pii
S0304-3835(20)30155-5journal_volume
480pub_type
杂志文章相关文献
CANCER LETTERS文献大全abstract：:The comparative effects of the subchronic administration to rats of ethionine-supplemented and of chemically defined methyl-deficient diets on the hepatic levels of S-adenosylmethionine (SAM) and of ornithine decarboxylase (ODC), an enzyme marker of cell proliferation, were studied. Both treatments led to decreased he...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(81)90018-5
更新日期：1981-08-01 00:00:00
abstract：:Not-targeted effects represent a paradigm shift from the "DNA centric" view that ionizing radiation only elicits biological effects and subsequent health consequences as a result of an energy deposition event in the cell nucleus. While this is likely true at higher radiation doses (>1 Gy), at low doses (<100 mGy) non-...
journal_title：Cancer letters
pub_type： 杂志文章,评审
doi：10.1016/j.canlet.2013.09.009
更新日期：2015-01-01 00:00:00
abstract：:The antitumor effects of 2'-C-cyano-2'-deoxy-1-beta-D- arabinofuranosylcytosine (CN-DAC), a synthetic 1-beta-D-arabinofuranosyl-cytosine (ara-C) derivative, were examined and compared with that of ara-C in murine tumors and in various human tumors using three different chemosensitivity tests. CNDAC extended the life s...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(92)90024-p
更新日期：1992-05-30 00:00:00
abstract：:Tumor angiogenesis is a critical step for the growth and metastasis of solid tumors. Vascular endothelial growth factor (VEGF) is the most important angiogenic molecule associated with tumor-induced neovascularization. VEGF exerts its activity through binding to its receptor tyrosine kinase, KDR/Flk-1, expressed on th...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2003.11.008
更新日期：2004-05-10 00:00:00
abstract：:Cancer stem cells (CSCs) are a sub-population of cancer cells that possess characteristics associated with normal stem cells but with the peculiarity that they are tumourigenic. This property allows them to persist in the tumour population, causing relapse and metastasis by giving rise to new tumours. Accordingly, if ...
journal_title：Cancer letters
pub_type： 杂志文章,评审
doi：10.1016/j.canlet.2012.04.020
更新日期：2013-09-10 00:00:00
abstract：:Neoxanthin and fucoxanthin, which have the characteristic structure of 5,6-monoepoxide and an allenic bond, were previously found to reduce the viability of human prostate cancer cells most intensively among 15 dietary carotenoids tested. In the present study, the induction of apoptosis in PC-3 cells by these two caro...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2004.07.048
更新日期：2005-03-18 00:00:00
abstract：:In this study we demonstrate the involvement of ras oncogenes in bladder cancer at the level of RNA overexpression. We examined 26 bladder specimens, consisting of paired tumor and adjacent normal tissue and found that H-ras transcripts were overexpressed in 39% of the specimens while K-ras and N-ras in 58% of total s...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(96)04372-8
更新日期：1996-10-22 00:00:00
abstract：:N-ethyl-N-nitrosourea-induced rat gliomas showed a stimulation of cytosolic spermidine N-acetyltransferase activity compared with normal brain, with an increased formation of N1 and N8-acetylspermidine, suggesting the activation of two enzymes acetylating the polyamine in N1 and N8 position, respectively. The enhancem...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(94)90387-5
更新日期：1994-01-30 00:00:00
abstract：:The effects of glucocorticoids and cholesterol on morphological transformation have been studied using hamster embryo cells. The cells were exposed sequentially to benzo[a]pyrene (BP) (3 days) and the tumor promotor 12-O-tetradecanoylphorbol-13-acetate (TPA) (4 days) before scoring for morphologically transformed colo...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(82)90039-8
更新日期：1982-02-01 00:00:00
abstract：:In the current study we examined the regulation of Bak, a death promoter of an apoptotic pathway, in the human breast cancer cell line MCF-7. We observed a time-dependent increase in both Bak mRNA and protein levels which appeared to correlate well with the increase in cell density. We also found that treatment of cel...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(97)00430-8
更新日期：1998-02-13 00:00:00
abstract：:The rationale for melanoma specific antitumor agents containing phenolic amines is based in part upon the ability of the enzyme tyrosinase to oxidize these prodrugs to toxic intermediates. Two phenolic amine compounds, N-acetyl-4-S-cysteaminylphenol (N-Ac-4-S-CAP) and 4-S-cysteaminylphenol (4-S-CAP), demonstrated grow...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(92)90092-a
更新日期：1992-03-31 00:00:00
abstract：:Many of the polycyclic aromatic hydrocarbons (e.g., benzo[a]pyrene (B[a]P), benzanthracene (BA), 3-methylcholanthrene (3-MC)) are not only carcinogenic, but also induce AHH in human tissues. Recently, chrysene has been implicated as an etiologic determinant of chemical carcinogenesis. Here we describe the ability of c...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(79)80059-2
更新日期：1979-10-01 00:00:00
abstract：:Post-transplant lymphoproliferative disorders (PTLDs) represent the most severe complication of both solid organ and hematopoietic stem cell transplantation. The Epstein-Barr Virus (EBV) is the main driver of PTLD, particularly those occurring early after transplantation. EBV-driven malignancies are associated with se...
journal_title：Cancer letters
pub_type： 杂志文章,评审
doi：10.1016/j.canlet.2015.08.007
更新日期：2015-12-01 00:00:00
abstract：:Deregulation of the cell-cycle G1-restriction point control via abnormalities of Rb-pathway components is a frequent event in the formation of cancer. The aim of this study was to evaluate numerical aberrations of the Cyclin D1 (CCND1, PRAD1, bcl-1) gene locus at chromosome 11q13 in basal cell carcinomas (BCCs) and sq...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2004.07.011
更新日期：2005-03-10 00:00:00
abstract：:Metabolic reprogramming such as the aerobic glycolysis or Warburg effect is well recognized as a common feature of tumorigenesis. However, molecular mechanisms underlying metabolic alterations for tumor therapeutic resistance are poorly understood. Through gene expression profiling analysis we found that histone H3K36...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2016.05.004
更新日期：2016-08-10 00:00:00
abstract：:Cell migration and invasion leading to metastasis is a major cause of death from endometrial cancer (EC). We have shown that the rate of EC cell migration is inversely related to the level of insulin-like growth factor protein-3 (IGFBP-3). Down-regulation of IGFBP-3 by siRNA in EC cells accelerated migration without a...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2011.11.011
更新日期：2012-04-01 00:00:00
abstract：:Oleanolic acid (OA, 3β-hydroxyolean-12-en-28-oic acid) is a ubiquitous pentacyclic multifunctional triterpenoid, widely found in several dietary and medicinal plants. Natural and synthetic OA derivatives can modulate multiple signaling pathways including nuclear factor-κB, AKT, signal transducer and activator of trans...
journal_title：Cancer letters
pub_type： 杂志文章,评审
doi：10.1016/j.canlet.2014.01.016
更新日期：2014-05-01 00:00:00
abstract：:In the present study, the question of whether the ubiquitous environmental pollutant, 1-nitropyrene (1-NP), can induce gamma-glutamyltranspeptidase (GGT)-positive foci, an early lesion occurring during hepatocarcinogenesis, when given orally to F344 rats was examined. Significant induction of GGT-positive foci was obs...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(89)90031-1
更新日期：1989-04-01 00:00:00
abstract：:Much of the interest on the chemopreventive properties of licorice has been focused on the plant genius Glycyrrhiza glabra. In this study the ethanol extract of Chinese licorice root, Glycyrrhiza uralensis (G. uralensis) was investigated for its estrogenic effect and the ability to inhibit cell proliferation in the MC...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2004.12.038
更新日期：2005-12-18 00:00:00
abstract：:Inhibition of DNA synthesis was observed and quantitated in hamster buccal pouch epithelium exposed in vivo and in vitro to indirect acting carcinogens. Topical application of a 0.5% solution of the potent hamster buccal pouch carcinogen 7,12-dimethylbenz[a]-anthracene (DMBA) acutely inhibited epithelial DNA synthesis...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(90)90210-o
更新日期：1990-09-01 00:00:00
abstract：:Protease inhibitors (PIs) of synthetic, bacterial or soybean origin have been shown to suppress carcinogen or radiation-induced rat mammary carcinogenesis. We report, for the first time, the effect of year-long feeding of Field bean meal, a rich source of PIs with a 24% protein content, at different protein levels in ...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(97)04721-6
更新日期：1997-06-03 00:00:00
abstract：:In experimental cancer chemoprevention studies, the doses of the agent used to produce an inhibitory response are generally very high, sometimes bordering on levels that will result in toxicity to the test animals. The present study was designed to test the hypothesis that low doses of two or more agents may be just a...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(88)90066-3
更新日期：1988-04-01 00:00:00
abstract：:Growing epidemiological evidence points out the carcinogenic hazard of inhaled aflatoxin B1 (AFB1) to the pulmonary system. Metabolism of AFB1 by lung microsomes and its binding to calf thymus DNA are reported for the first time in this paper. In addition, the ability of dietary butylated hydroxyanisole (BHA) to modul...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/0304-3835(88)90261-3
更新日期：1988-05-01 00:00:00
abstract：:Currently, animal models are used to test the efficacy of tumor treatment. A significant reduction of tumor mass is lauded as great improvement. As we begin the 21st century, one wonders if this is sufficient and acceptable for cancer treatment. Although the presence of cancer stem cell (CSCs) is not a new phenomenon,...
journal_title：Cancer letters
pub_type： 杂志文章,评审
doi：10.1016/j.canlet.2014.03.002
更新日期：2015-01-28 00:00:00
abstract：:In the present study, Tbx3, a member of the T-box family of transcription factors, was identified as an up-regulated gene by mRNA differential display in the regression (apoptosis) stage after uracil-induction of papillomatosis in the rat urinary bladder. Immunohistochemical analysis revealed that apoptosis cells are ...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2004.07.051
更新日期：2005-02-28 00:00:00
abstract：:Apoptosis plays a role in the elimination of DNA-damaged cells thus protecting the host from cancer development. Some data indicate that normal variations within the sequence of apoptotic genes may lead to suboptimal apoptotic capacity and therefore increased cancer risk. We tested 19 coding apoptotic gene SNPs in 2-s...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2009.01.012
更新日期：2009-06-18 00:00:00
abstract：:Osteosarcoma is the most common primary bone tumour in children and adolescents and advanced osteosarcoma patients with evidence of metastasis share a poor prognosis. Osteosarcoma frequently gains resistance to standard therapies highlighting the need for improved treatment regimens and identification of novel therape...
journal_title：Cancer letters
pub_type： 杂志文章,评审
doi：10.1016/j.canlet.2016.11.019
更新日期：2017-02-01 00:00:00
abstract：:The molecular chaperone Hsp90 is not only of major current interest in fundamental biological research but also recognised as an exciting new target for the treatment of cancer and other diseases. In addition to playing an important role in response to proteotoxic heat shock and others stresses, Hsp90 is also critical...
journal_title：Cancer letters
pub_type： 杂志文章,评审
doi：10.1016/j.canlet.2003.08.032
更新日期：2004-04-08 00:00:00
abstract：:Glypican-5 (GPC5) may be a potential tumor suppressor gene in non-small cell lung cancer (NSCLC). The present study aimed to clarify the GPC5 expression pattern and to explore its potential functions in NSCLC. The expression of GPC5 gene was lower in lung cancer tissues compared with adjacent noncancerous tissues. The...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/j.canlet.2013.08.020
更新日期：2013-12-01 00:00:00
abstract：:In this study we combined flow cytometry with fluorescence in situ hybridization to detect numerical aberrations in chromosomes. Fifty-nine human malignant gliomas were examined by flow cytometry for DNA-content and cell cycle analysis and for numerical aberrations of chromosome 1 by in situ hybridization using a chro...
journal_title：Cancer letters
pub_type： 杂志文章
doi：10.1016/s0304-3835(98)00383-8
更新日期：1999-04-26 00:00:00
