Abstract：
:Although the use of metabarcoding to identify taxa in DNA mixtures is widely approved, its reliability in quantifying taxon abundance is still the subject of debate. In this study we investigated the relationships between the amount of pollen grains in mock solutions and the abundance of high-throughput sequence reads and how the relationship was affected by the pollen counting methodology, the number of PCR cycles, the type of markers and plant species whose pollen grains have different characteristics. We found a significant positive relationship between the number of DNA sequences and the number of pollen grains in the mock solutions. However, better relationships were obtained with light microscopy as a pollen grain counting method compared with flow cytometry, with the chloroplastic trnL marker compared with ribosomal ITS1 and with 30 when compared with 25 or 35 PCR cycles. We provide a list of recommendations to improve pollen quantification.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Baksay S,Pornon A,Burrus M,Mariette J,Andalo C,Escaravage Ndoi
10.1038/s41598-020-61198-6subject
Has Abstractpub_date
2020-03-06 00:00:00pages
4202issue
1issn
2045-2322pii
10.1038/s41598-020-61198-6journal_volume
10pub_type
杂志文章abstract：:In the present work, a catalyst-free physical vapour deposition method is used to synthesize high yield of Bi2Se3 nanoribbons. By replacing standard glass or quartz substrates with aluminium covered with ultrathin porous anodized aluminium oxide (AAO), the number of synthesized nanoribbons per unit area can be increas...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47547-0
更新日期：2019-08-05 00:00:00
abstract：:The objective was to measure the association between badger culling and bovine tuberculosis (TB) incidents in cattle herds in three areas of England between 2013-2017 (Gloucestershire and Somerset) and 2015-2017 (Dorset). Farming industry-selected licensed culling areas were matched to comparison areas. A TB incident ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49957-6
更新日期：2019-10-11 00:00:00
abstract：:An aberrant expression of integrin β1 has been implicated in breast cancer progression. Here, we compared the cell behaviors of wild-type (WT), β1 gene deleted (KO), and β1 gene restored (Res) MDA-MB-231 cells. Surprisingly, the expression of β1 exhibited opposite effects on cell proliferation. These effects were depe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18430
更新日期：2016-01-05 00:00:00
abstract：:The therapeutic value of FK228 as a cancer treatment option is well known, and various types of cancer have been shown to respond to this drug. However, the complete mechanism of FK228 and the affect it has on histone lysine acetylation and the colon cancer cell proteome are largely unknown. In the present study, we u...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18443
更新日期：2015-12-17 00:00:00
abstract：:Near-critical plasmas irradiated at ultra-high laser intensities (I > 1018W/cm2) allow to improve the performances of laser-driven particle and radiation sources and to explore scenarios of great astrophysical interest. Near-critical plasmas with controlled properties can be obtained with nanostructured low-density ma...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22147-6
更新日期：2018-03-01 00:00:00
abstract：:Optimized mesoporous photocatalyst endowed with high specific surface area and large pore size was synthesized by sol-gel method. These large pore mesoporous materials (33.39 nm) were conducive to the movement of larger molecules or groups in pore path and for effective use of active sites. The high specific surface a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29902
更新日期：2016-07-19 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-62442-9
更新日期：2020-04-02 00:00:00
abstract：:Acute leukemia (AL) patients undergoing intensive induction chemotherapy develop severe gut dysbiosis, placing them at heightened risk for infectious complications. Some AL patients will undergo "repeat therapy" (re-induction or salvage) due to persistent or relapsed disease. We hypothesized that prior injury to the m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42652-6
更新日期：2019-04-15 00:00:00
abstract：:The topotactic phase transition in SrCoO x (x = 2.5-3.0) makes it possible to reversibly transit between the two distinct phases, i.e. the brownmillerite SrCoO2.5 that is a room-temperature antiferromagnetic insulator (AFM-I) and the perovskite SrCoO3 that is a ferromagnetic metal (FM-M), owing to their multiple valen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16246-z
更新日期：2017-11-22 00:00:00
abstract：:Nanomedicine development currently suffers from a lack of efficient tools to predict pharmacokinetic behavior without relying upon testing in large numbers of animals, impacting success rates and development costs. This work presents dendPoint, the first in silico model to predict the intravenous pharmacokinetics of d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51789-3
更新日期：2019-10-29 00:00:00
abstract：:In this study, we made a comparative efficacy and safety assessment of two different fixed combinations of drugs, viz., tafluprost/timolol (TAF/TIM) and latanoprost/carteolol (LAT/CAR), by determining their effects on intraocular pressure (IOP) in ocular normotensive monkeys and examining their toxic effects on ocular...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44028-2
更新日期：2019-05-16 00:00:00
abstract：:Zinc oxy-selenide Zn(O,Se) is a novel material, that can replace the toxic CdS buffer layer in thin film solar cells and other optoelectronic devices. In this paper a systematic study of the structural, optical and electrical properties of Zn(O,Se) layers, grown by pulsed laser deposition under 50 mTorr of nitrogen ba...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54008-1
更新日期：2019-11-25 00:00:00
abstract：:Antimicrobial resistant infections are a serious public health threat worldwide. Whole genome sequencing approaches to rapidly identify pathogens and predict antibiotic resistance phenotypes are becoming more feasible and may offer a way to reduce clinical test turnaround times compared to conventional culture-based m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18972-w
更新日期：2018-01-11 00:00:00
abstract：:Parasitic lice (order Phthiraptera) infest birds and mammals. The typical animal mitochondrial (mt) genome organization, which consists of a single chromosome with 37 genes, was found in chewing lice in the suborders Amblycera and Ischnocera. The sucking lice (suborder Anoplura) known, however, have fragmented mt geno...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17389
更新日期：2015-11-30 00:00:00
abstract：:The chronic unpredictable stress (CUS) paradigm is extensively used in preclinical research. However, CUS exhibits translational inconsistencies, some of them resulting from the use of adult rodents, despite the evidence that vulnerability for many psychiatric disorders accumulates during early life. Here, we assessed...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53624-1
更新日期：2019-11-22 00:00:00
abstract：:Hypertension is the most common and lethal risk factor for cardiovascular disease (CVD). Numerous variants have been associated with hypertension, however, most of which failed to get replication due to ethnic differences. In this study, we analyzed associations of 10 newly reported single nucleotide polymorphisms (SN...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15245
更新日期：2015-10-21 00:00:00
abstract：:Meniscal tears have a poor healing capacity, and damage to the meniscus is associated with significant pain, disability, and progressive degenerative changes in the knee joint that lead to osteoarthritis. Therefore, strategies to promote meniscus repair and improve meniscus function are needed. The objective of this s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44855-3
更新日期：2019-06-18 00:00:00
abstract：:The objective of this study was to assess the diagnosis value of urinary inflammatory index (UII) and systemic immune-inflammation index (SII) for UTI. Nine inflammatory indexes including neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, SII and six UIIs were calculated for Receiver operating characteristi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76352-3
更新日期：2020-11-09 00:00:00
abstract：:In this work, the circular dichroisms (CD) of nanorice heterodimers consisting of two parallel arranged nanorices with the same size but different materials are investigated theoretically. Symmetry-breaking is introduced by using different materials and oblique incidence to achieve strong CD at the vicinity of Fano re...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16069
更新日期：2015-11-05 00:00:00
abstract：:This study was aimed to assess the relationship of intraocular forward scattering, corneal backward scattering, and corneal higher-order aberrations (HOAs) with corrected distance visual acuity (CDVA) in eyes with granular corneal dystrophy (GCD). We retrospectively examined forty two eyes of 42 consecutive patients w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24677
更新日期：2016-04-18 00:00:00
abstract：:Sea surface temperature (SST) patterns can - as surface climate forcing - affect weather and climate at large distances. One example is El Niño-Southern Oscillation (ENSO) that causes climate anomalies around the globe via teleconnections. Although several studies identified and characterized these teleconnections, ou...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45423-5
更新日期：2019-06-19 00:00:00
abstract：:Chemokine receptor 5 (CCR5) belongs to G protein coupled receptors (GPCRs) and plays an important role in treatment of human immunodeficiency virus (HIV) infection since HIV uses CCR5 protein as a co-receptor. Recently, the crystal structure of CCR5-bound complex with an approved anti-retroviral drug (maroviroc) was r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13180
更新日期：2015-08-24 00:00:00
abstract：:Protein phosphorylation is one of the most prevalent and well-understood protein modifications. Oxidation of protein-bound methionine, which has been traditionally perceived as an inevitable damage derived from oxidative stress, is now emerging as another modification capable of regulating protein activity during stre...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40403
更新日期：2017-01-12 00:00:00
abstract：:Ovarian cancer is a highly aggressive disease and novel treatments are required. Thyroid hormones binding to αvβ3 integrin produced growth-promoting activities in ovarian cancer and we hypothesized that natural thyroid hormone derivatives may antagonize these actions. The effect of three antagonists, tetraiodoacetic a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16593-x
更新日期：2017-11-28 00:00:00
abstract：:Conventional crystalline β-MnO2 usually exhibits poor electrochemical activities due to the narrow tunnels in its rutile-type structure. In this study, we synthesized a novel 2D β-MnO2 network with long-range order assembled by β-MnO2 nanowires and demonstrated that the novel 2D β-MnO2 network exhibits enhanced electr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02193
更新日期：2013-01-01 00:00:00
abstract：:The addition of a small amount of dopant impurities to crystals is a common method to tune the properties of materials. Usually the doping grade is restricted by the low solubility of the dopants; increasing the doping concentration beyond this solubility limit leads to supersaturated solutions in which dopant cluster...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12730-8
更新日期：2017-10-03 00:00:00
abstract：:Alcohol consumption before or during pregnancy poses serious health risks to the fetus; however, the underlying mechanisms involved remain obscure. Here, we investigated whether ethanol consumption before pregnancy affects maternal or fetal health and whether pharmacological inhibition of CYP2E1, a major ethanol oxida...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66971-1
更新日期：2020-06-22 00:00:00
abstract：:Drought is one of the major impediments in wheat productivity. Traditional breeding and marker assisted QTL introgression had limited success. Available wheat genomic and RNA-seq data can decipher novel drought tolerance mechanisms with putative candidate gene and marker discovery. Drought is first sensed by root tiss...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49915-2
更新日期：2019-09-26 00:00:00
abstract：:Botulinum neurotoxins (BoNTs) are highly potent toxins that cleave neuronal SNARE proteins required for neurotransmission, causing flaccid paralysis and death by asphyxiation. Currently, there are no clinical treatments to delay or reverse BoNT-induced blockade of neuromuscular transmission. While aminopyridines have ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16064-3
更新日期：2017-11-20 00:00:00
abstract：:The phase behaviour of a system composed of amino acid-based surfactant (sodium N-lauroylsarcosinate hydrate), 1-decanol and deionised water was investigated for vesicle formation. Changing the molar ratio of the amphiphiles, two important aggregate structures were observed in the aqueous corner of the phase diagram. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00071
更新日期：2011-01-01 00:00:00
