Abstract:
:Meniscal tears have a poor healing capacity, and damage to the meniscus is associated with significant pain, disability, and progressive degenerative changes in the knee joint that lead to osteoarthritis. Therefore, strategies to promote meniscus repair and improve meniscus function are needed. The objective of this study was to generate porcine meniscus-derived matrix (MDM) scaffolds and test their effectiveness in promoting meniscus repair via migration of endogenous meniscus cells from the surrounding meniscus or exogenously seeded human bone marrow-derived mesenchymal stem cells (MSCs). Both endogenous meniscal cells and MSCs infiltrated the MDM scaffolds. In the absence of exogenous cells, the 8% MDM scaffolds promoted the integrative repair of an in vitro meniscal defect. Dehydrothermal crosslinking and concentration of the MDM influenced the biochemical content and shear strength of repair, demonstrating that the MDM can be tailored to promote tissue repair. These findings indicate that native meniscus cells can enhance meniscus healing if a scaffold is provided that promotes cellular infiltration and tissue growth. The high affinity of cells for the MDM and the ability to remodel the scaffold reveals the potential of MDM to integrate with native meniscal tissue to promote long-term repair without necessarily requiring exogenous cells.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ruprecht JC,Waanders TD,Rowland CR,Nishimuta JF,Glass KA,Stencel J,DeFrate LE,Guilak F,Weinberg JB,McNulty ALdoi
10.1038/s41598-019-44855-3subject
Has Abstractpub_date
2019-06-18 00:00:00pages
8719issue
1issn
2045-2322pii
10.1038/s41598-019-44855-3journal_volume
9pub_type
杂志文章abstract::The digit ratio has been used as a retrospective noninvasive biomarker to investigate the putative effects of prenatal exposure to androgens. In recent years, many scholars have paid attention to the association between 2D:4D (the second and fourth digits) and prostatic cancer. This study explored the prognostic signi...
journal_title:Scientific reports
pub_type: 杂志文章,多中心研究
doi:10.1038/s41598-017-05638-w
更新日期:2017-07-12 00:00:00
abstract::Inspired by the well-documented tumor protecting ability of paullones, recently, we synthesized novel paullone-like scaffolds, indole-fused benzo-oxazepines (IFBOs), and screened them against hepatocellular carcinoma (HCC) specific Hep-G2 cells. Three of the synthesized compounds significantly attenuated the progressi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24288-0
更新日期:2018-04-12 00:00:00
abstract::Chlorpyrifos is one of the most widely used pesticides that acts on the nervous system by inhibiting acetylcholinesterase. Prolonged use of chlorpyrifos causes severe neurological, autoimmune, and persistent developmental disorders in humans. Therefore, in this study, a highly sensitive and robust biosensor platform w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36746-w
更新日期:2019-01-22 00:00:00
abstract::Safe management of high level nuclear waste is a worldwide significant issue for which vitrification has been selected by many countries. There exists a crucial need for improving our understanding of the ageing of the glass under irradiation. While external irradiation by ions provides a rapid simulation of damage in...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25499
更新日期:2016-05-05 00:00:00
abstract::Insight refers to the sudden conscious shift in the perception of a situation following a period of unconscious processing. The present study aimed to investigate the implicit neural mechanisms underlying insight-based recognition, and to determine the association between these mechanisms and the extent of pupil dilat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25207-z
更新日期:2018-05-02 00:00:00
abstract::The Near-infrared Fluorescence (NIRF) molecular imaging of cancer is known to be superior in sensitivity, deeper penetration, and low phototoxicity compared to other imaging modalities. In view of an increased need for efficient and targeted imaging agents, we synthesized a NAD(P)H quinone oxidoreductase 1 (NQO1)-acti...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44111-8
更新日期:2019-06-12 00:00:00
abstract::Mouse embryonic stem cells (ESCs) and epiblast stem cells (EpiSCs) represent naive and primed pluripotency states, respectively, and are maintained in vitro by specific signalling pathways. Furthermore, ESCs cultured in serum-free medium with two kinase inhibitors (2i-ESCs) are thought to be the ground naïve pluripote...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23822-4
更新日期:2018-04-10 00:00:00
abstract::An optical radon detection method is presented. Radon decay is directly measured by observing the secondary radiolumines cence light that alpha particles excite in air, and the selectivity of coincident photon detection is further enhanced with online pulse-shape analysis. The sensitivity of a demonstration device was...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21532
更新日期:2016-02-12 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-29056-8
更新日期:2018-07-18 00:00:00
abstract::Multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows simultaneous imaging of numerous RNA species in their native cellular environment and hence spatially resolved single-cell transcriptomic measurements. However, the relatively modest brightness of signals from single RNA molecules can become ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43943-8
更新日期:2019-05-22 00:00:00
abstract::Despite recent advances, the myocardial microstructure remains imperfectly understood. In particular, bundles of cardiomyocytes have been observed but their three-dimensional organisation remains debated and the associated mechanical consequences unknown. One of the major challenges remains to perform multiscale obser...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76820-w
更新日期:2020-11-25 00:00:00
abstract::The underlying reasons behind the unprecedented increase of the mortality rates due to the opioid epidemics in the United States are still not fully uncovered. Most efforts have been focused on targeting opioids, but there is little information about vulnerable populations at high risk of opioid abuse and death. In th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61281-y
更新日期:2020-03-09 00:00:00
abstract::Acanthamoeba can cause visually destructive Acanthamoeba keratitis (AK) in contact lens (CL) users. The purpose of this study was to determine whether Acanthamoeba was present in the CL cases of CL wearers and to develop techniques to prevent the contaminations. To accomplish this, 512 CL case samples were collected f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69554-2
更新日期:2020-07-28 00:00:00
abstract::The morbidity and mortality rates of human immunodeficiency virus (HIV)-hepatitis B virus (HBV) coinfection are higher than that of either infection alone. Outcomes and the virological response to antiretrovirals (combination antiretroviral therapy, cART) were explored in HIV/HBV subjects in a cohort of Italian patien...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46976-1
更新日期:2019-08-16 00:00:00
abstract::The activation and regulation of coagulation Factor XIII (FXIII) protein has been the subject of active research for the past three decades. Although discrete evidence exists on various aspects of FXIII activation and regulation a combinatorial structure/functional view in this regard is lacking. In this study, we pre...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30105
更新日期:2016-07-25 00:00:00
abstract::When making decisions, humans can maximize the positive outcome of their actions by choosing the option associated with the highest reward. We have recently shown that choices modulate effects of reward via a bias in spatial attention: Locations associated with a lower reward are anticipatorily suppressed, as indicate...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42255-1
更新日期:2019-04-08 00:00:00
abstract::The tibial tuberosity-trochlear groove (TT-TG) distance is a radiographic measurement that is used to quantify malalignment of the patellofemoral joint (PFJ) in cross-sectional imaging. There is an ongoing debate about the impact of the TT-TG-distance on lateral patellar instability and the initiating of cartilage deg...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72299-7
更新日期:2020-09-18 00:00:00
abstract::Fluorescent proteins (FP) are used to study various biological processes. Recently, a series of near-infrared (NIR) FPs based on bacterial phytochromes was developed. Finding ways to improve NIR FPs is becoming progressively important. By applying rational design and molecular evolution we have engineered R. palustris...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18348
更新日期:2015-12-18 00:00:00
abstract::The preferential degeneration of Substantia nigra dopamine midbrain neurons (SN DA) causes the motor-symptoms of Parkinson's disease (PD). Voltage-gated L-type calcium channels (LTCCs), especially the Cav1.3-subtype, generate an activity-related oscillatory Ca(2+) burden in SN DA neurons, contributing to their degener...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13688
更新日期:2015-09-18 00:00:00
abstract::This is the first pharmacological characterisation of a neuropeptide G protein-coupled receptor (GPCR) in a crustacean. We cloned the ORF of the red pigment-concentrating hormone from a German strain of Daphnia pulex (Dappu-RPCH), as well as that of the cognate receptor (Dappu-RPCHR). Dappu-RPCHR has the hallmarks of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06805-9
更新日期:2017-07-31 00:00:00
abstract::Previous studies reported that boreal winter precipitation in southeastern China (SEC) tends to increase during El Niño. In this study, however, we find that most weak El Niño events are accompanied by below-normal precipitation in SEC, although strong El Niño events are accompanied by above-normal precipitation in SE...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53496-5
更新日期:2019-11-22 00:00:00
abstract::Accumulating evidence indicates that cancer cells show specific alterations in phospholipid metabolism that contribute to tumour progression in several types of cancer, including colorectal cancer. Questions still remain as to what lipids characterize the outer edge of cancer tissues and whether those cancer outer edg...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep29935
更新日期:2016-07-20 00:00:00
abstract::Pigment organelles known as melanosomes disperse or aggregate in a melanophore in response to hormones. These movements are mediated by the microtubule motors kinesin-2 and cytoplasmic dynein. However, the force generation mechanism of dynein, unlike that of kinesin, is not well understood. In this study, to address t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41458-w
更新日期:2019-03-25 00:00:00
abstract::Drug metabolites usually have structures of split-ring resonators (SRRs), which might lead to negative permittivity and permeability in electromagnetic field. As a result, in the UV-vis region, the latent fingermarks images of drug addicts and non drug users are inverse. The optical properties of latent fingermarks ar...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20336
更新日期:2016-02-03 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-019-46785-6
更新日期:2019-07-17 00:00:00
abstract::The bronchoscopy, though usually safe, is occasionally associated with complications, such as pneumonia. However, the use of prophylactic antibiotics is not recommended by the guidelines of the British Thoracic Society. Thus far there are few reports of the risk factors for post-bronchoscopy pneumonia; the purpose of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76998-z
更新日期:2020-11-17 00:00:00
abstract::Recently, amorphous Hf-B-Si-C-N coatings found to demonstrate superior high-temperature oxidation resistance. The microstructure evolution of two coatings, Hf7B23Si22C6N40 and Hf6B21Si19C4N47, annealed to 1500 °C in air is investigated to understand their high oxidation resistance. The annealed coatings develop a two-...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-40428-6
更新日期:2019-03-05 00:00:00
abstract::Coral reefs across the world's oceans are in the midst of the longest bleaching event on record (from 2014 to at least 2016). As many of the world's reefs are remote, there is limited information on how past thermal conditions have influenced reef composition and current stress responses. Using satellite temperature d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38402
更新日期:2016-12-06 00:00:00
abstract::Climate influences vegetation directly and through climate-mediated disturbance processes, such as wildfire. Temperature and area burned are positively associated, conditional on availability of vegetation to burn. Fire is a self-limiting process that is influenced by productivity. Yet, many fire projections assume su...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39284-1
更新日期:2019-02-26 00:00:00
abstract::Recent evidence suggests that some seizures are preceded by preictal changes that start from minutes to hours before an ictal event. Nevertheless an adequate statistical evaluation in a large database of continuous multiday recordings is still missing. Here, we investigated the existence of preictal changes in long-te...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04545
更新日期:2014-04-01 00:00:00