Abstract:
:The therapeutic value of FK228 as a cancer treatment option is well known, and various types of cancer have been shown to respond to this drug. However, the complete mechanism of FK228 and the affect it has on histone lysine acetylation and the colon cancer cell proteome are largely unknown. In the present study, we used stable isotope labeling by amino acids in cell culture (SILAC) and affinity enrichment followed by high-resolution liquid chromatograph-mass spectrometer (LC-MS)/MS analysis to quantitate the changes in the lysine acetylome in HCT-8 cells after FK228 treatment. A total of 1,194 lysine acetylation sites in 751 proteins were quantified, with 115 of the sites in 85 proteins being significantly upregulated and 38 of the sites in 32 proteins being significantly downregulated in response to FK228 treatment. Interestingly, 47 histone lysine acetylation sites were identified in the core histone proteins. We also found a novel lysine acetylation site on H2BK121. These significantly altered proteins are involved in multiple biological functions as well as a myriad of metabolic and enzyme-regulated pathways. Taken together, the link between FK228 function and the downstream changes in the HCT-8 cell proteome observed in response to FK228 treatment is established.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Wang TY,Jia YL,Zhang X,Sun QL,Li YC,Zhang JH,Zhao CP,Wang XY,Wang Ldoi
10.1038/srep18443subject
Has Abstractpub_date
2015-12-17 00:00:00pages
18443issn
2045-2322pii
srep18443journal_volume
5pub_type
杂志文章abstract::Three dimensional (3D) microvascular imaging of cubic millimeter to centimeter size volumes often requires much time and expensive instruments. By combining optical clearing with a novel scatter-based optical coherence tomography (OCT) contrast agent, we have greatly extended OCT imaging depth in excised tissues while...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37313-z
更新日期:2019-01-29 00:00:00
abstract::Differential host responses may be critical determinants of distinct pathologies of West Nile virus (WNV) NY99 (pathogenic) and WNV Eg101 (non-pathogenic) strains. We employed RNA-seq technology to analyze global differential gene expression in WNV-infected mice brain and to identify the host cellular factors leading ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26350
更新日期:2016-05-23 00:00:00
abstract::The controversial anti-proliferative effects of Echinacea purpurea (L.) Moench (Asteraceae) might be related to different plant metabolites contained in plant samples, extracts and products. The influence of bacterial endophytes on the synthesis of bioactive compounds in the medicinal plants has been previously demons...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47329-8
更新日期:2019-07-26 00:00:00
abstract::This study aimed to determine ANGPTL3 serum levels in healthy young lean and obese non-diabetic men during an oral glucose tolerance test (OGTT) and correlate them with anthropometric, biochemical and hormonal parameters. A case-control study was carried out and 30 young obese non-diabetic (23.90 ± 3.84 years and BMI ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77961-8
更新日期:2020-12-03 00:00:00
abstract::The poor control of the adhesion of TiO2 nanotubes (TNTs) layers to a non-anodized titanium (Ti) substrate has limited their widespread application, because the stripping mechanism has not yet been revealed. Here, we report a novel method to control the detachment of TNTs by post-treatment of the as-fabricated samples...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07178
更新日期:2014-11-24 00:00:00
abstract::Recent work reveals that the extinction of conditioned fear depends upon the interval between conditioning and extinction. Extinction training that takes place within minutes to hours after fear conditioning fails to produce a long-term extinction memory, a phenomenon known as the immediate extinction deficit (IED). N...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46010-4
更新日期:2019-07-01 00:00:00
abstract::Photonic structure designs based on optimization algorithms provide superior properties compared to those using intuition-based approaches. In the present study, we numerically and experimentally demonstrate subwavelength focusing of light using wavelength scale absorption-free dielectric scattering objects embedded i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30871
更新日期:2016-08-01 00:00:00
abstract::Bacterial communities in wastewater treatment plants (WWTPs) affect plant functionality through their role in the removal of pollutants from wastewater. Bacterial communities vary extensively based on plant operating conditions and influent characteristics. The capacity of WWTPs can also affect the bacterial community...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50952-0
更新日期:2019-10-15 00:00:00
abstract::A three-dimensional multiscale modeling framework is developed to analyze the failure procedure of radially aligned zinc oxide (ZnO) enhanced single fiber composites (SFC) under tensile loading to understand the interfacial improvement between the fiber and the matrix. The model introduces four levels in the computati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56503-x
更新日期:2019-12-27 00:00:00
abstract::The present study evaluated the effects of exposing liquid-stored boar semen to different red light LED regimens on sperm quality and reproductive performance. Of all of the tested photo-stimulation procedures, the best pattern consisted of 10 min light, 10 min rest and 10 min of further light (10-10-10 pattern). This...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22569
更新日期:2016-03-02 00:00:00
abstract::Src kinase is known to regulate fibroblast migration. However, the contribution of integrin and Src kinase interaction to lung fibrosis has not been mechanistically investigated. Our data demonstrate that integrin alpha v (αV) recruited Src kinase and that leads to subsequent Src activation in fibroblasts plated on fi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46357
更新日期:2017-04-11 00:00:00
abstract::Several zebrafish strains such as AB, Tübingen (TU), Wild India Kolkata (WIK) and Tupfel long fin (TL) have been established for genetic study. Each strain has its morphological and behavioral traits. Motor traits, however, have not been explored in zebrafish strains. We here applied a treadmill for fish (swimmill) an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52592-w
更新日期:2019-11-08 00:00:00
abstract::The zebrafish has the capacity to regenerate its heart after severe injury. While the function of a few genes during this process has been studied, we are far from fully understanding how genes interact to coordinate heart regeneration. To enable systematic insights into this phenomenon, we generated and integrated a ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26822
更新日期:2016-05-31 00:00:00
abstract::Transient ischemia produces postischemic tingling sensation. Ischemia also produces nerve conduction block that may modulate spinal neural circuits. In the present study, reduced mechanical thresholds for hindpaw-withdrawal reflex were found in mice after transient hindpaw ischemia, which was produced by a high pressu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11191
更新日期:2015-07-13 00:00:00
abstract::Cardiovascular diseases are directly linked to smoking habits, which has both physiological and anatomical effects on the systemic and retinal circulations, and these changes can be detected with fundus photographs. Here, we aimed to 1- design a Convolutional Neural Network (CNN), using retinal photographs, to differe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43670-0
更新日期:2019-05-09 00:00:00
abstract::Electrochemical capacitors (EC) have received tremendous interest due to their high potential to satisfy the urgent demand in many advanced applications. The development of new electrode materials is considered to be the most promising approach to enhance the EC performance substantially. Herein, we present a high-cap...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep06083
更新日期:2014-08-15 00:00:00
abstract::Hydrocarbons fuel our economy. Furthermore, intermediate goods and consumer products are often hydrocarbon-based. Beside all the progress they made possible, hydrogen-containing substances can have severe detrimental effects on materials exposed to them. Hydrogen-assisted failure of iron alloys has been recognised mor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60370-2
更新日期:2020-02-27 00:00:00
abstract::Real-time impairment of ocular blood flow (OBF) under common carotid artery stenosis (CCAS) has not been ascertained. We aimed to longitudinally assess the impact of CCAS on OBF using a rabbit model. About 75% stenosis was created by tying the common carotid artery with a plastic mandrel using a nylon suture. The plas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72556-9
更新日期:2020-09-28 00:00:00
abstract::Recent studies suggest that glypican-1 (GPC-1) is a biomarker for prostate cancer, but there are few studies elucidating the role of GPC-1 in prostate cancer progression. We observed high expression of GPC-1 in more aggressive prostate cancer cell lines such as PC-3 and DU-145. While inhibition of GPC-1 expression in ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47874-2
更新日期:2019-08-07 00:00:00
abstract::Fungus-growing termites forage dead plant materials from the field to cultivate symbiotic Termitomyces fungi in the nest. Termite foraging behavior and the entry of symbiotic arthropod inquilines may transfer nematodes into a nest and adversely affect fungus production. To test whether nematodes were transferred to fu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44993-8
更新日期:2019-06-20 00:00:00
abstract::The fabrication of Ru nanostructures by focused electron beam induced deposition (FEBID) requires suitable precursor molecules and processes to obtain the pure metal. So far this is problematic because established organometallic Ru precursors contain large organic ligands, such as cyclopentadienyl anions, that tend to...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67803-y
更新日期:2020-07-02 00:00:00
abstract::Lattice dynamics and elasticity for the high-temperature ε phase (body-centered cubic; bcc) of plutonium is predicted utilizing first-principles electronic structure coupled with a self-consistent phonon method that takes phonon-phonon interaction and strong anharmonicity into account. These predictions establish the ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01034-6
更新日期:2017-04-25 00:00:00
abstract::As an emerging sterilization technology, cold atmospheric plasma offers a dry, non-thermal, rapid process that is minimally damaging to a majority of substrates. However, the mechanisms by which plasma interacts with living cells are poorly understood and the plasma generation apparatuses are complex and resource-inte...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-35166-0
更新日期:2018-11-12 00:00:00
abstract::The catalytic and optical properties of metal nanoparticles can be combined to create platforms for light-driven chemical energy storage and enhanced in-situ reaction monitoring. However, the heavily damped plasmon resonances of many catalytically active metals (e.g. Pt, Pd) prevent this dual functionality in pure nan...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17431
更新日期:2015-11-30 00:00:00
abstract::Garcinia L. (Clusiaceae) fruits are a rich source of (-)-hydroxycitric acid, and this has gained considerable attention as an anti-obesity agent and a popular weight loss food supplement. In this study, we assessed adulteration of morphologically similar samples of Garcinia using DNA barcoding, and used NMR to quantif...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28635-z
更新日期:2018-07-12 00:00:00
abstract::Routers, switches, and repeaters are essential components of modern information-processing systems. Similar devices will be needed in future superconducting quantum computers. In this work we investigate experimentally the time evolution of Autler-Townes splitting in a superconducting phase qubit under the application...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00645
更新日期:2012-01-01 00:00:00
abstract::Drug addiction remains a prevalent and fatal disease worldwide that carries significant social and economic impacts. Recent reports suggest illicit pregabalin (Lyrica) use may be increasing among youth, however the addictive potential of pregabalin has not been well established. Drug seeking behavior and chronic drug ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51556-4
更新日期:2019-10-22 00:00:00
abstract::Acute-on-chronic liver failure (ACLF) is a dynamic syndrome, and sequential assessments can reflect its prognosis more accurately. Our aim was to build and validate a new scoring system to predict short-term prognosis using baseline and dynamic data in ACLF. We conducted a retrospective cohort analysis of patients wit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81431-0
更新日期:2021-01-19 00:00:00
abstract::Etomoxir (ETO) is a widely used small-molecule inhibitor of fatty acid oxidation (FAO) through its irreversible inhibitory effects on the carnitine palmitoyl-transferase 1a (CPT1a). We used this compound to evaluate the role of fatty acid oxidation in rapidly proliferating T cells following costimulation through the C...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-24676-6
更新日期:2018-04-19 00:00:00
abstract::A carbon fibre is a rod-like microstructure, the longitudinal axis of which is aligned with the orientation of the director in a nematic liquid crystal. A nematic liquid crystal with negative dielectric anisotropy is mixed with carbon fibres. By applying an electric field perpendicular to the director, the carbon fibr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56594-6
更新日期:2019-12-27 00:00:00