Abstract：
:The activation of the majority of AGC kinases is regulated by two phosphorylation events on two conserved serine/threonine residues located on the activation loop and on the hydrophobic motif, respectively. In AGC kinase family, phosphomimetic substitutions with aspartate or glutamate, leading to constitutive activation, have frequently occurred at the hydrophobic motif site. On the contrary, phosphomimetic substitutions in the activation loop are absent across the evolution of AGC kinases. This observation is explained by the failure of aspartate and glutamate to mimic phosphorylatable serine/threonine in this regulatory site. By detailed 3D structural simulations of RSK2 and further biochemical evaluation in cells, we show that the phosphomimetic residue on the activation loop fails to form a critical salt bridge with R114, necessary to reorient the αC-helix and to activate the protein. By a phylogenetic analysis, we point at a possible coevolution of a phosphorylatable activation loop and the presence of a conserved positively charged amino acid on the αC-helix. In sum, our analysis leads to the unfeasibility of phosphomimetic substitution in the activation loop of RSK and, at the same time, highlights the peculiar structural role of activation loop phosphorylation.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Somale D,Di Nardo G,di Blasio L,Puliafito A,Vara-Messler M,Chiaverina G,Palmiero M,Monica V,Gilardi G,Primo L,Gagliardi PAdoi
10.1038/s41598-019-56937-3subject
Has Abstractpub_date
2020-01-17 00:00:00pages
591issue
1issn
2045-2322pii
10.1038/s41598-019-56937-3journal_volume
10pub_type
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