Abstract:
:Passive immunotherapy may have particular benefits for the treatment of severe influenza infection in at-risk populations, however little is known of the impact of passive immunotherapy on the formation of memory responses to the virus. Ideally, passive immunotherapy should attenuate the severity of infection while still allowing the formation of adaptive responses to confer protection from future exposure. In this study, we sought to determine if administration of influenza-specific ovine polyclonal antibodies could inhibit adaptive immune responses in a murine model of lethal influenza infection. Ovine polyclonal antibodies generated against recombinant PR8 (H1N1) hemagglutinin exhibited potent prophylactic capacity and reduced lethality in an established influenza infection, particularly when administered intranasally. Surviving mice were also protected against reinfection and generated normal antibody and cytotoxic T lymphocyte responses to the virus. The longevity of ovine polyclonal antibodies was explored with a half-life of over two weeks following a single antibody administration. These findings support the development of an ovine passive polyclonal antibody therapy for treatment of severe influenza infection which does not affect the formation of subsequent acquired immunity to the virus.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Stevens NE,Hatjopolous A,Fraser CK,Alsharifi M,Diener KR,Hayball JDdoi
10.1038/srep29154subject
Has Abstractpub_date
2016-07-06 00:00:00pages
29154issn
2045-2322pii
srep29154journal_volume
6pub_type
杂志文章abstract::We developed a reporter system that can be used in a dual manner in visualizing mature osteoblast formation. The system is based on a helper-dependent adenoviral vector (HDAdV), in which a fluorescent protein, Venus, is expressed under the control of the 19-kb human osteocalcin (OC) genomic locus. By infecting human a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46105-y
更新日期:2019-07-04 00:00:00
abstract::Endothelium-dependent flow-mediated dilation (ED-FMD), a biophysical marker of endothelial dysfunction, is apparently impaired in patients with systemic lupus erythematosus (SLE) but such observation is inconsistent. Here, we assessed and compared the brachial artery ED-FMD (baED-FMD) using ultrasonography between SLE...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析
doi:10.1038/s41598-017-07574-1
更新日期:2017-08-04 00:00:00
abstract::We propose a new efficient algorithm to simulate magnetic structures numerically. It contains a generative model using a complex-valued neural network to generate k-space information. The output information is hermitized and transformed into real-space spin configurations through an inverse fast Fourier transform. The...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-53411-y
更新日期:2019-11-13 00:00:00
abstract::Computed tomography (CT) and magnetic resonance imaging (MRI) can quantify muscle mass and quality. However, it is still unclear if CT and MRI derived measurements can be used interchangeable. In this prospective study, fifty consecutive participants of a cancer screening program underwent same day low-dose chest CT a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68797-3
更新日期:2020-07-16 00:00:00
abstract::Attending a location in space facilitates responses to targets at that location when the time between cue and target is short. Certain types of exogenous cues - such as sudden peripheral onsets - have been described as reflexive and automatic. Recent studies however, have been showing many cases where exogenous cues a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31633-w
更新日期:2018-09-05 00:00:00
abstract::This study evaluated the 4-year results of 32 patients with T1N0 low-lying rectal adenocarcinoma treated solely with californium-252 (Cf-252) neutron intracavity brachytherapy (ICBT). Patients were solicited into the study from January 2008 to June 2011. All the patients had refused surgery or surgery was contraindica...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40619
更新日期:2017-01-17 00:00:00
abstract::Principal research on energy from thermonuclear fusion uses Deuterium-Tritium plasmas magnetically trapped in toroidal devices. As major scientific problem for an economic (i.e., really feasible) reactor, we must understand how to lead strongly heated plasmas to sustain a high fusion gain while large fraction of curre...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-27996-9
更新日期:2018-07-09 00:00:00
abstract::Demands for tomato local varieties are increasing worldwide, especially in organic farming mainly for their high sensory value and attractive appearance. This is the case of the "Moruno" tomato type, widely grown in the Mediterranean countries and greatly due to its highly appreciated organoleptic attributes but low y...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41018-2
更新日期:2019-03-14 00:00:00
abstract::A new species of fossil conifer wood, Podocarpoxylon donghuaiense sp. nov., is described from the late Eocene of Nadu Formation in Baise Basin of the Guangxi Province, South China. This fossil wood is characterized by distinct growth rings, circular to oval tracheids in cross section, 1-2-seriate opposite pits on radi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32294
更新日期:2016-08-30 00:00:00
abstract::Endocannabinoids are amphiphilic molecules that play crucial neurophysiological functions acting as lipid messengers. Antagonists and knockdown of the classical CB1 and CB2 cannabinoid receptors do not completely abolish many endocannabinoid activities, supporting the idea of a mechanism independent of receptors whose...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41362
更新日期:2017-01-27 00:00:00
abstract::Immune-evasion and immune checkpoints are promising new therapeutic targets for several cancer entities. In ovarian cancer, the clinical role of programmed cell death receptor ligand 1 (PD-L1) expression as mechanism to escape immune recognition has not been clarified yet. We analyzed PD-L1 expression of primary ovari...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42929
更新日期:2017-03-07 00:00:00
abstract::Reactive oxygen species (ROS) participate in various physiological and pathological functions following generation from different types of cells. Here we explore ROS functions on spontaneous tail regeneration using gecko model. ROS were mainly produced in the skeletal muscle after tail amputation, showing a temporal i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20752
更新日期:2016-02-08 00:00:00
abstract::Genes with similar roles in the cell cluster on chromosomes, thus benefiting from coordinated regulation. This allows gene function to be inferred by transferring annotations from genomic neighbors, following the guilt-by-association principle. We performed a systematic search for co-occurrence of >1000 gene functions...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55984-0
更新日期:2019-12-20 00:00:00
abstract::Cerebral lateralization is expressed at both the structural and functional levels, and can exist as either a stable characteristic or as a dynamic feature during behavior and development. The anatomically relatively simple olfactory system demonstrates lateralization in both human and non-human animals. Here, we explo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15109-x
更新日期:2017-11-06 00:00:00
abstract::Culture expanded mesenchymal stromal cells (MSCs) are being extensively studied for therapeutic applications, including treatment of graft-versus-host disease, osteogenesis imperfecta and for enhancing engraftment of hematopoietic stem cells after transplantation. Thus far, clinical trials have shown that the therapeu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50955-x
更新日期:2019-10-07 00:00:00
abstract::Memory function deficits induced by Alzheimer's disease (AD) are believed to be one of the causes of an increased risk of tripping in patients. Working memory contributes to accurate stepping over obstacles during locomotion, and AD-induced deficits of this memory function may lead to an increased risk of contact with...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07220
更新日期:2014-11-27 00:00:00
abstract::The gene encoding the cytosolic β-glucosidase GBA3 shows pseudogenization due to a truncated allele (rs358231) that is polymorphic in humans. Since this enzyme is involved in the transformation of many plant β-glycosides, this particular case of gene loss may have been influenced by dietary adaptations during evolutio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68106-y
更新日期:2020-07-14 00:00:00
abstract::Grafting of commercial tomato varieties and hybrids on the tomato ecotype Manduria resulted in high levels of tolerance to the infection of Sw5 resistance-breaking strains of tomato spotted wilt virus and of severe cucumber mosaic virus strains supporting hypervirulent satellite RNAs that co-determine stunting and nec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59421-5
更新日期:2020-02-13 00:00:00
abstract::Due to their small dimensions, electrophysiology on thin and intricate axonal branches in support of understanding their role in normal and diseased brain function poses experimental challenges. To reduce experimental complexity, we coupled microelectrode arrays (MEAs) to bi-level microchannel devices for the long-ter...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09033-3
更新日期:2017-08-17 00:00:00
abstract::Although most of the near non-equilibrium microstructures of alloys produced by laser powder bed fusion (LPBF) additive manufacturing (AM) are being reported at a rapid rate, the accountable thermokinetics of the entire process have rarely been studied. In order to exploit the versatility of this AM process for the de...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63281-4
更新日期:2020-05-05 00:00:00
abstract::BMPs are multifunctional growth factors implicated in regulating the ovarian function as key intra-ovarian factors. Biological effects of BMPs are mediated through binding with membrane bound receptors like BMPR-IB and initiating downstream Smad signaling pathway. FecB mutation, regarded as a loss of function mutation...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-77596-9
更新日期:2020-11-24 00:00:00
abstract::Förster Resonance Energy Transfer (FRET) allows for the visualization of nanometer-scale distances and distance changes. This sensitivity is regularly achieved in single-molecule experiments in vitro but is still challenging in biological materials. Despite many efforts, quantitative FRET in living samples is either r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62924-w
更新日期:2020-04-16 00:00:00
abstract::We report on a new colorimetric DNA detection method that takes advantage of the power of polymerase chain reaction (PCR) and the simplicity of the classic litmus test. The strategy makes use of a modified set of primers for PCR to facilitate ensuing manipulations of resultant DNA amplicons: their tagging with urease ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03009-z
更新日期:2017-06-08 00:00:00
abstract::The 'brain regionalization genes' Six3/6, Otx, Pax2/5/8, Gbx, and Hox1 are expressed in a similar fashion in the deuterostome, ecdysozoan, and the cephalopod brain, questioning whether this holds also true for the remaining Mollusca. We investigated developmental Gbx-expression in representatives of both molluscan sis...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05605-5
更新日期:2017-07-14 00:00:00
abstract::The aesthetic value of landscapes contributes to human well-being. However, studies which have investigated the link between biodiversity and ecosystem services have not taken aesthetic value into account. In this study we evaluated how the aesthetics of coralligenous reefs, a key marine ecosystem in the Mediterranean...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34229
更新日期:2016-09-28 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-30083-8
更新日期:2018-08-02 00:00:00
abstract::Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease. We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15717-7
更新日期:2017-11-10 00:00:00
abstract::The goal of this study was to develop a cheap and simple medium and to optimize fermentation parameters for fibrinolytic enzyme production by Bacillus subtilis WR350. A low-cost medium containing 35 g/L sucrose, 20 g/L corn steep powder and 2 g/L MgSO4·7H2O was developed via single-factor and orthogonal experiments. A...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-43371-8
更新日期:2019-05-02 00:00:00
abstract::Candida albicans (C. albicans) is the most common cause of candidiasis in humans and animals. This study was established to a new experimental infection model for systemic candidiasis using partridge and embryonated partridge egg. First, we tested the induction of systemic candidiasis in partridge and embryonated part...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81592-y
更新日期:2021-01-22 00:00:00
abstract::The natural bacterial diversity is regarded as a treasure trove for natural products. However, accessing complex cell mixtures derived from environmental samples in standardized high-throughput screenings is challenging. Here, we present a droplet-based microfluidic platform for ultrahigh-throughput screenings able to...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31263-2
更新日期:2018-08-30 00:00:00