Repetitive DNA landscape in essential A and supernumerary B chromosomes of Festuca pratensis Huds.

Abstract:

:Here, we characterized the basic properties of repetitive sequences in essential A and supernumerary B chromosomes of Festuca pratensis Huds. This was performed by comparative analysis of low-pass Illumina sequence reads of B chromosome lacking (-B) and B chromosome containing (+B) individuals of F. pratensis. 61% of the nuclear genome is composed of repetitive sequences. 43.1% of the genome are transposons of which DNA transposons and retrotransposons made up 2.3% and 40.8%, respectively. LTR retrotransposons are the most abundant mobile elements and contribute to 40.7% of the genome and divided into Ty3-gypsy and Ty1-copia super families with 32.97% and 7.78% of the genome, respectively. Eighteen different satellite repeats were identified making up 3.9% of the genome. Five satellite repeats were used as cytological markers for chromosome identification and genome analysis in the genus Festuca. Four satellite repeats were identified on B chromosomes among which Fp-Sat48 and Fp-Sat253 were specific to the B chromosome of F. pratensis.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Ebrahimzadegan R,Houben A,Mirzaghaderi G

doi

10.1038/s41598-019-56383-1

subject

Has Abstract

pub_date

2019-12-27 00:00:00

pages

19989

issue

1

issn

2045-2322

pii

10.1038/s41598-019-56383-1

journal_volume

9

pub_type

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