Abstract：
:The aim of this study was to develop a novel approach allowing simultaneous enrichment as well as specific and fast detection of Escherichia coli O157:H7 by indirect ELISA using optimized support membrane based on chitosan (CHI), cellulose nanocrystals (CNCs), and glycerol (GLY). Therefore, combining the step of the capture of the pathogen and enrichment steps for the microbial growth led to a high detection signal at a low inoculation level without cross-reaction with Pseudomonas and Salmonella strains. The detection was performed by varying incubation periods and different level of inoculations. The signal of detection in samples incubated with the chitosan-based support reinforced with CNCs and directly from E. coli O157:H7 bacterial culture was much higher as compared to CNCs-free support with cell-free supernatant samples. The CCG support reinforced with 0.6% CNCs improved the detection signal of E. coli O157:H7 by 25% compared to control. The whole bacterial culture showed a higher immobilization signal than unfiltered and cell-free supernatant. The spider web trap approach (SWTA) detect E. coli O157:H7 after only 4 h of enrichment compared to 24 h with conventional methods. The adjustment of this innovative SWTA could minimize the risks of cross-contamination and consequently, food product recalls by facilitating significantly the detection of foodborne pathogens in samples collected from food surface, tools and work surfaces in food processing industries.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Baraketi A,D'Auria S,Shankar S,Fraschini C,Salmieri S,Menissier J,Lacroix Mdoi
10.1016/j.ijbiomac.2019.09.225subject
Has Abstractpub_date
2020-03-01 00:00:00pages
1009-1014eissn
0141-8130issn
1879-0003pii
S0141-8130(19)36750-9journal_volume
146pub_type
杂志文章abstract：:Creatine kinase (CK) is a key enzyme involved in intracellular energy homeostasis. The distinct tissue distribution of muscle CK (MMCK) and brain CK (BBCK) implies that they function under conditions facing dissimilar environmental stresses. We found that MMCK and BBCK were significantly different in their stability a...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2010.03.025
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abstract：:The research presented aims at developing Ropinirole hydrochloride (RHCl) nanoemulsion (NE) with nigella oil for Parkinson's disease (PD). In silico study was done to explore interactions of ropinirole and thymoquinone at receptor site (TNF-α and NFK-β). Ropinirole and Thymoquinone forms a hydrogen bond with residue A...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.11.207
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.12.038
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abstract：:To fabricate a biomimetic nanostructured bicomponent scaffolds, two types of chitin/silk fibroin (SF) nanofibrous scaffolds (blend scaffolds and hybrid scaffolds) were prepared by electrospinning or simultaneous electrospinning of chitin/SF solutions. The chitin/SF bicomponent scaffolds were after-treated with water v...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2007.12.004
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abstract：:2,2',4,4'-tetrahydroxybenzophenone (Uvinul D50), a sunscreen used in cosmetics, has two effects in the melanin biosynthesis pathway. On the one hand, it acts a weak inhibitor of tyrosinase and on the other, it accelerates the conversion of dopachrome to melanin. Uvinul D50 was seen to behave as a weak competitive inhi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.02.032
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abstract：:Three well-formed film combinations of chitosan, in the beta form and cellulose acetate biopolymer, having different proportions, have been synthesized and characterized by X-ray diffraction and infrared spectroscopy. The film having a 1.0/0.50 proportion presented 6.87 mmol of nitrogen atoms per gram of synthesized h...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2005.04.001
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abstract：:Several pectin derivatives were prepared by chemical modifications of the polysaccharide with natural fatty acids. The obtained biodegradable pectin-based materials, pectin-linoleate, pectin-oleate and pectin-palmitate, were investigated for their antimicrobial activity against several bacterial strains, Staphylococcu...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.04.011
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abstract：:Aggregation properties of Gelatin chains in neutral aqueous solutions, are reported in the temperature range T = 35-60 degrees C, from the measured intrinsic viscosity [eta], diffusion coefficient, D(o), molecular weight Mw, and radius of gyration (Rg) data. Gelatin chains doubled their size as the solution was cooled...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(98)00003-8
更新日期：1998-07-01 00:00:00
abstract：:Bacterial vaginosis (BV) is a common type of vaginal inflammation caused by a proliferation of pathogenic bacteria, among which Mobiluncus curtisii. In our previous studies on M. curtisii genome, we identified the presence of a genomic fragment encoding a 25 kDa pore-forming toxin, the CAMP factor, which is known to b...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2020-05-01 00:00:00
abstract：:The aim of this study was to develop a new bioactive gelatin-oxidized starch nanofibers containing Lawsonia Inermis (henna) for treating second-degree burn wounds. Continuous, smooth, and bead-free fibers were obtained when the gelatin-starch ratio was 70/30.Additionally, the fiber dimeter was reduced with increasing ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.10.061
更新日期：2018-02-01 00:00:00
abstract：:Modified biopolymer chitosan namely 2-hydroxy-1-naphthaldehyde chitosan (CTS-Nap) has been synthesized for the removal of toxic chromium from aqueous solutions. In an attempt to enhance the adsorption capacity of toxic chromium on the prepared modified biopolymer, magnetic Fe3O4 nanoparticles have been loaded on the m...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.12.188
更新日期：2021-02-15 00:00:00
abstract：:Xyloglucan endotransglycosylase/hydrolase (XTH) belongs to the GH16 subfamily of the glycoside hydrolases of carbohydrate active enzymes and plays an important role in the structure and function of plant cell walls. In this study, 11 members of the XTH gene family were cloned from Populus tomentosa. A bioinformatics a...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.11.099
更新日期：2020-07-15 00:00:00
abstract：:Three fractions of the endo-polysaccharides from the mycelium of Hericium erinaceus (HEP) grown on tofu whey were obtained by the fractional precipitation with gradient concentrations of ethanol (HEP40, HEP60 and HEP80). The chemical and physical characteristics of the three crude polysaccharides were investigated by ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.09.002
更新日期：2012-12-01 00:00:00
abstract：:Gamma radiation was used as the initiator/crosslinker agent for the synthesis of thermo-sensitive hydrogel networks, under the form of membranes, using dextran and N-isopropylacrylamide. The prepared membranes were loaded with Ondansetron™, a potent antiemetic drug and tested as drug delivery systems. The characteriza...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.07.002
更新日期：2013-10-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2015.12.004
更新日期：2016-03-01 00:00:00
abstract：:Large amounts of DNA-enriched biomaterials, such as salmon milts and shellfish gonads, are discarded as industrial waste around the world. Therefore, the utilizations of DNA with the specific function are important for the biomaterial science and the curce technology. We could convert the discarded DNA to an enzymatic...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2008.03.005
更新日期：2008-06-01 00:00:00
abstract：:A polyether-urethane based on polytetrahydrofuran containing hydroxypropyl cellulose for biomedical applications was tested for its biocompatibility. Ketoprofen was incorporated (3% and 6%) in the polyurethane matrix as an anti-inflammatory drug. Dynamic vapour sorption method was employed for testing the water sorpti...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.09.026
更新日期：2013-01-01 00:00:00
abstract：:In this work, various new nano-alkalinecellulose carboxylates (NACCs) were prepared by oxidative hydrolysis of microalkalinecellulose (MACp) given from prewashed cotton. After the optimization of the reaction time, temperature, oxidant system, and oxidant loading, the new flake-shape anionic NACC24 with base capacity ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.05.024
更新日期：2019-08-01 00:00:00
abstract：:This study reports encapsulation-vitrification of Leydig cells. The Leydig cells were encapsulated in sodium alginate beads of different sizes and cryopreserved by vitrification or slow freezing. Physico-chemical characterization of beads was done by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (X...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.02.233
更新日期：2020-06-15 00:00:00
abstract：:Bacterial cellulose (BC) derived materials represents major advances to the current regenerative and diagnostic medicine. BC is a highly pure, biocompatible and versatile material that can be utilized in several applications - individually or in the combination with different components (e.g. biopolymers and nanoparti...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2017.05.171
更新日期：2017-11-01 00:00:00
abstract：:In this study, we investigated the protective effect and possible mechanism of a polysaccharide (CCP) from Coptis chinensis against Amyloid-β protein (Aβ)-induced toxicity in PC12 cells. The results showed pretreatment with CCP significantly protected PC12 cells from Aβ25-35 induced cell death, lactate dehydrogenase (...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.05.041
更新日期：2019-08-01 00:00:00
abstract：:In this study, the fluid uptake (swelling) kinetics and disintegrant properties of gellan gum in metronidazole tablets were evaluated in both simulated gastric and intestinal fluids (SGF and SIF respectively) without enzymes. The mechanical properties as well as the disintegration and dissolution profile of the tablet...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2010.05.005
更新日期：2010-08-01 00:00:00
abstract：:In the present study, a bacterium resistance to heavy metals was isolated from contaminated areas. An eco-friendly and simple method was found to biosynthesis of silver nanoparticles (AgNPs) by reducing of aqueous Ag+ using the heavy metals resistance MKH1 bacterium. The biosynthesized AgNPs were characterized by UV-v...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.08.179
更新日期：2018-02-01 00:00:00
abstract：:Isomaltulose production by bacterial fermentation was limited, due to generation of undesirable products and reduced yields. Isomaltulose production using sucrose isomerase (SIase) catalyzed methods was expected to be more applicable, but was hampered by low SIase activity and lack of a secreted SIase producer. Here, ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.10.010
更新日期：2019-01-01 00:00:00
abstract：:A novel oligomeric SGNH-arylesterase (Sm23) from Sinorhizobium meliloti 1021 was characterized using biochemical and biophysical methods. A sequence comparison of Sm23 with other SGNH members confirmed the presence of catalytic triad (Ser(10), Asp(187), and His(190)) and oxyanion holes (Ser(10)-Gly(50)-Asn(90)). The w...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2009.12.010
更新日期：2010-03-01 00:00:00
abstract：:Understanding the molecular mechanisms of azo dye decolorization is important for the development of effective bioremediation for textile-colored wastewater. A halophilic bacterium Halomonas sp. strain GT was isolated, which could degrade the azo dye Acid Brilliant Scarlet GR at 10% NaCl. The complete genome sequence ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.11.175
更新日期：2019-02-15 00:00:00
abstract：:Biopolymers are widely used for the removal of chromium from aqueous medium but it possesses limitations like poor sorption capacity and low stability. To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chrom...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.10.010
更新日期：2016-02-01 00:00:00
abstract：:Bacillus licheniformis LMG 7559, which is capable of producing extracellular poly gamma- (glutamic acid) (PGA), was provided for the biopolymer synthesis. Using a modified PGA medium for PGA production, the isolated biopolymer, undergone dialysis process mainly for desalination and removal of other impurities. The bac...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.10.090
更新日期：2020-03-15 00:00:00
abstract：:Wound dressings require good antiseptic properties, mechanical strength and, more trustably, natural material ingredients. Antimicrobial properties of cerium ions and chitosan are known and alginate based wound dressings are commercially available. In this study, the advantages of these materials were combined and alg...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.07.144
更新日期：2017-12-01 00:00:00
abstract：:In the present work, the atomic force microscopy (AFM) studies and rheological properties of aqueous solutions of microcrystalline chitosan (MCCh), polyacrylamide (PAM) and their mixtures at different weight ratios have been investigated. Flow measurements were carried out using on solutions of native polymers and the...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.05.026
更新日期：2015-08-01 00:00:00
