Overexpression of secreted sucrose isomerase in Yarrowia lipolytica and its application in isomaltulose production after immobilization.


:Isomaltulose production by bacterial fermentation was limited, due to generation of undesirable products and reduced yields. Isomaltulose production using sucrose isomerase (SIase) catalyzed methods was expected to be more applicable, but was hampered by low SIase activity and lack of a secreted SIase producer. Here, we aimed to obtain high levels of secreted SIase by overexpressing the SIase gene from Pantoea dispersa UQ68J in Yarrowia lipolytica, a successful host for efficient secretory expression, with a newly characterized strong constitutive promoter. After optimization of the culture medium, the engineered strain JD secreted SIase with an activity of 49.3 U/mL. The recombinant SIase was effectively immobilized onto polyvinyl alcohol-alginate, and the enzymatic activity recovery rate was up to 82.4%. The stability of the SIase was significantly improved by immobilization. Batch production of isomaltulose catalyzed by the immobilized SIase was performed under optimal conditions, generating 620.7 g/L isomaltulose with a yield of 0.96 g/g. The conversion rate of sucrose after 13 batches remained above 90%. These results demonstrated that the proposed SIase expression and immobilization method was promising in the industrial production of isomaltulose.


Int J Biol Macromol


Zhang P,Wang ZP,Liu S,Wang YL,Zhang ZF,Liu XM,Du YM,Yuan XL




Has Abstract


2019-01-01 00:00:00












  • Rapid self-association of highly amyloidogenic H-fragments of insulin: Experiment and molecular dynamics simulations.

    abstract::The so-called 'H-fragment' of insulin is an extremely amyloidogenic double chain peptide consisting of the N-terminal parts of A-chain and B-chain linked by a disulfide bond between Cys-7A and Cys-7B. Here, we conduct a detailed investigation of the self-association behavior of H-fragment monomers into amyloid-like fi...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Dec R,Koliński M,Kouza M,Dzwolak W

    更新日期:2020-05-01 00:00:00

  • Preparation and characterization of chitosan/polyacrylic acid/copper nanocomposites and their impact on onion production.

    abstract::Chitosan nanoparticles (CS-NPs) and chitosan/polyacrylic acid hydrogel nanoparticles (CS/PAA-HNPs) were obtained by ionic gelation with tripolyphosphate anions and copolymerization of CS with acrylic acid (AA), respectively. The prepared NPs were loaded by different concentrations of copper (1, 2 and 3% with respect t...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Abd El-Aziz ME,Morsi SMM,Salama DM,Abdel-Aziz MS,Abd Elwahed MS,Shaaban EA,Youssef AM

    更新日期:2019-02-15 00:00:00

  • Influences of different drying methods on the structural characteristics and multiple bioactivities of polysaccharides from okra (Abelmoschus esculentus).

    abstract::In this study, in order to evaluate the influences of drying methods on the chemical structures and bioactivities of polysaccharides from okra (OPPs), four drying methods, including microwave drying at 400 W, 600 W, and 800 W, freezing drying, hot air drying, and vacuum drying, were applied to dry okra fruits. Six dif...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Yuan Q,He Y,Xiang PY,Huang YJ,Cao ZW,Shen SW,Zhao L,Zhang Q,Qin W,Wu DT

    更新日期:2020-03-15 00:00:00

  • Radiation-mediated molecular weight reduction and structural modification in carrageenan potentiates improved photosynthesis and secondary metabolism in peppermint (Mentha piperita L.).

    abstract::In an attempt to gain insights into the possible relationship between the irradiation-mediated molecular weight reduction and structural modification and the growth-promotion activity, characterization of the polysaccharide before and after irradiation was carried out through Fourier Transform Infrared (FT-IR), Ultrav...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Ahmad B,Jahan A,Sadiq Y,Shabbir A,Jaleel H,Khan MMA

    更新日期:2019-03-01 00:00:00

  • Cell wall metabolism and autolytic activities of the yeast Saccharomyces exiguus.

    abstract::Autolytic activities were measured in cell walls prepared from the yeast Saccharomyces exiguus. Walls of yeast cells exhibited higher autolytic activities directed toward glucans at the exponential phase of growth when compared to cells at the stationary phase, while glucanase activities in the soluble extract fractio...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Inouhe M,Sugo E,Tohoyama H,Joho M,Nevins DJ

    更新日期:1997-08-01 00:00:00

  • Efficient adsorption of methylene blue and lead ions in aqueous solutions by 5-sulfosalicylic acid modified lignin.

    abstract::As a kind of biomass that exists widely in plants, lignin shows much diversity as a functional material. In order to improve the adsorption ability, lignin was chemically modified by 5-sulfosalicylic acid and then used to adsorb methylene blue (MB) and Pb2+ from aqueous solutions. The results showed that the 5-sulfosa...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Jin Y,Zeng C,Lü QF,Yu Y

    更新日期:2019-02-15 00:00:00

  • The differences between fungal α-glucan synthase determining pullulan synthesis and that controlling cell wall α-1,3 glucan synthesis.

    abstract::The fungal α-glucan synthases (Agss) are multi-domain proteins catalyzing biosynthesis of cell wall α-1,3-glucan which determines cell wall integrity or fungal pathogenicity and pullulan which is a maltotriosyl polymer made of α-1,4 and α-1,6 bound glucose units. The Agss family can be divided into 11 groups, some of ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Qi CY,Jia SL,Wei X,Yang G,Chi Z,Liu GL,Hu Z,Chi ZM

    更新日期:2020-11-01 00:00:00

  • Fabrication of Z-scheme photocatalysts g-C3N4/Ag3PO4/chitosan for the photocatalytic degradation of ciprofloxacin.

    abstract::In the present study, we have fabricated a novel chitosan based nanocomposite (g-C3N4/Ag3PO4/CS) containing g-C3N4 and Ag3PO4 nanoparticles. The fabricated nanocomposite was characterized successfully and used as a catalyst for photocatalytic degradation of ciprofloxacin (CIP) under visible light. The TEM results reve...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Alhokbany NS,Mousa R,Naushad M,Alshehri SM,Ahamad T

    更新日期:2020-12-01 00:00:00

  • Use of cellulose nanofibril (CNF)/silver nanoparticles (AgNPs) composite in salt hydrate phase change material for efficient thermal energy storage.

    abstract::Salt hydrate phase change materials (PCMs) possess the challenge of supercooling, which must be addressed to allow more efficient energy storage and utilisation. In this work, cellulose nanofibril (CNF), a versatile biopolymer was used to support and disperse silver nanoparticles (AgNPs), and the synthesised CNF/AgNPs...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Shen Z,Oh K,Kwon S,Toivakka M,Lee HL

    更新日期:2021-01-30 00:00:00

  • Enhanced activity and stability of papain by covalent immobilization on porous magnetic nanoparticles.

    abstract::Papain enzyme was successfully immobilized by covalent bonding onto biocompatible Fe3O4/SF nanoparticles, which were prepared with the soft template of silk fibroin (SF). The optimized immobilization condition is pH6.0, hydrolysis time of 60min, and an enzyme/support ratio of 10.0mg/g. Compared with free papain, the i...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Sheng W,Xi Y,Zhang L,Ye T,Zhao X

    更新日期:2018-07-15 00:00:00

  • Development of sustainable magnetic chitosan biosorbent beads for kinetic remediation of arsenic contaminated water.

    abstract::We report the preparation of both control chitosan and magnetic chitosan beads as biosorbents using chitosan as matrix and magnetite (Fe3O4) nanoparticles as reinforcement followed by detailed advanced characterization. The batch trials were performed to study the adsorption kinetics of biosorbents by removing As(III)...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Ayub A,Raza ZA,Majeed MI,Tariq MR,Irfan A

    更新日期:2020-11-15 00:00:00

  • Lycium barbarum polysaccharides grafted with doxorubicin: An efficient pH-responsive anticancer drug delivery system.

    abstract::Lycium barbarum polysaccharide-doxorubicin (LBP-DOX) nanoparticles were synthesized via a one-step Schiff base reaction between oxidized Lycium barbarum polysaccharide (oxidized LBP) and doxorubicin (DOX). The structure of LBP-DOX nanoparticles was characterized by multi-spectral methods, 1H nuclear magnetic resonance...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Wang Y,Bai F,Luo Q,Wu M,Song G,Zhang H,Cao J,Wang Y

    更新日期:2019-01-01 00:00:00

  • Preparation of formyl cellulose and its enhancement effect on the mechanical and barrier properties of polylactic acid films.

    abstract::Cellulose was modified by formic acid to prepare formyl cellulose (FC). The amount of formyl groups in FC was adjusted by controlling the reaction time, reaction temperature, and formic acid concentration. Then, FC was used to reinforce polylactic acid (PLA) films prepared by solution casting. Scanning electron micros...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Long S,Zhong L,Lin X,Chang X,Wu F,Wu R,Xie F

    更新日期:2021-01-09 00:00:00

  • The oxidation of potato starch by Electro-Fenton system in the presence of Fe(II) ions.

    abstract::An efficient method was developed to prepare oxidized potato starch under the pasting point by Electro-Fenton system, in which a complex of sodium citrate and Fe2+ (SC-Fe2+) was used as catalyst, and H2O2 was used as oxidant. In the preparation process the oxidation degree of starch was controlled by the molar ratios ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Dang X,Chen H,Shan Z,Zhen W,Yang M

    更新日期:2019-01-01 00:00:00

  • Isolation, cloning and characterization of an azoreductase and the effect of salinity on its expression in a halophilic bacterium.

    abstract::Understanding the molecular mechanisms of azo dye decolorization is important for the development of effective bioremediation for textile-colored wastewater. A halophilic bacterium Halomonas sp. strain GT was isolated, which could degrade the azo dye Acid Brilliant Scarlet GR at 10% NaCl. The complete genome sequence ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Tian F,Guo G,Zhang C,Yang F,Hu Z,Liu C,Wang SW

    更新日期:2019-02-15 00:00:00

  • Isolation, structural characterization and bioactivities of polysaccharides and its derivatives from Auricularia-A review.

    abstract::Auricularia mushrooms are known for their bioactive compounds, mostly polysaccharides, which have numerous biological activities, such as antioxidant, antitumor, immunomodulatory, hyperlipidemic, antidiabetic, anticoagulant and hepatoprotective effects. Over the past decades, there has been a consistent focus on the i...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审


    authors: Miao J,Regenstein JM,Qiu J,Zhang J,Zhang X,Li H,Zhang H,Wang Z

    更新日期:2020-05-01 00:00:00

  • Purification and structural characterization of a novel antioxidant and antibacterial protein from Arca inflata.

    abstract::A novel protein (J2-C4) with antioxidant and antibacterial activities was purified from the edible portion of Arca inflata. The purity of J2-C4 was measured to be 97.62% by RP-HPLC analysis, and the molecular weight was 20,537.0 Da by ESI-MS/MS. The isoelectric point of J2-C4 was determined to be 5.18 by IEF-PAGE. Sec...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Wang W,Shi H,Zhu J,Li C,Song L,Yu R

    更新日期:2018-09-01 00:00:00

  • Methylglyoxal induced glycation and aggregation of human serum albumin: Biochemical and biophysical approach.

    abstract::Serum protein glycation and formation of advanced glycation end products (AGEs) correlates with many diseases viz. diabetes signifying the importance of studying the glycation pattern of serum proteins. In our present study, methylglyoxal was investigated for its effect on the structure of human serum albumin (HSA); e...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Ahmed A,Shamsi A,Khan MS,Husain FM,Bano B

    更新日期:2018-07-01 00:00:00

  • Sustainable panels based on starch bioadhesives: An insight into structural and tribological performance.

    abstract::This work was focused on evaluating the effects of polycarboxylic acid addition and retrogradation phenomenon on tribological performance, thermal, and mechanical properties of both, bioadhesives and sustainable panels. The rheological behavior of adhesives was affected by retrogradation, exhibiting formulations conta...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Monroy Y,Seré P,Rivero S,García MA

    更新日期:2020-04-01 00:00:00

  • Sulfated polysaccharides as bioactive agents from marine algae.

    abstract::Recently, much attention has been paid by consumers toward natural bioactive compounds as functional ingredients in nutraceuticals. Marine algae are considered as valuable sources of structurally diverse bioactive compounds. Marine algae are rich in sulfated polysaccharides (SPs) such as carrageenans in red algae, fuc...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审


    authors: Ngo DH,Kim SK

    更新日期:2013-11-01 00:00:00

  • Chitin hoops obtained from Ommatoiulus sabulosus (Diplopoda, Julidae) used for bovine serum albumin adsorption.

    abstract::A new hoop shaped three dimensional chitin was obtained successfully from the body segment of a diplopod species (Ommatoiulus sabulosus) by following the procedure decolorization, demineralization and deproteinization. Purity of the hoop shaped three-dimensional chitin was proved by FT-IR analysis and chitinase digest...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Šatkauskienė I,Jarusevičiūtė S,Baublys V,Maheta M,Tubelytė V

    更新日期:2017-01-01 00:00:00

  • Structural features of polysaccharides from edible jambo (Syzygium jambos) fruits and antitumor activity of extracted pectins.

    abstract::Jambo is a tropical fruit cultivated in Southeast Asia and tropical regions of America and Africa. After extraction, its polysaccharides were structurally characterized. Water soluble polysaccharides (WSP) were composed of GalA:Ara:Gal:Glc:Rha in 51:22:16:5:6 molar ratio, indicating the presence of pectic polysacchari...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Tamiello CS,Adami ER,de Oliveira NMT,Acco A,Iacomini M,Cordeiro LMC

    更新日期:2018-10-15 00:00:00

  • Light-induced phosphorylation of crystallins in the retinal pigment epithelium.

    abstract::Protein phosphorylations have essential regulatory roles in visual signaling. Previously, we found that phosphorylation of several proteins in the retina and retinal pigment epithelium (RPE) is involved in anti-apoptotic signaling under oxidative stress conditions, including light exposure. In this study, we used a ph...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Lee H,Chung H,Lee SH,Jahng WJ

    更新日期:2011-01-01 00:00:00

  • Cyclodextrin based ternary system of modafinil: Effect of trimethyl chitosan and polyvinylpyrrolidone as complexing agents.

    abstract::Modafinil is an approved drug for the treatment of narcolepsy and have a strong market presence in many countries. The drug is widely consumed for off-label uses and currently listed as a restricted drug. Modafinil has very low water solubility. To enhance the aqueous solubility of modafinil by the formation of a tern...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Patel P,Agrawal YK,Sarvaiya J

    更新日期:2016-03-01 00:00:00

  • Superhydrophobic cotton fabrics coated by chitosan and titanium dioxide nanoparticles with enhanced antibacterial and UV-protecting properties.

    abstract::Superhydrophobic cotton fabrics were fabricated using chitosan/titanium dioxide (TiO2) nanocomposites. Morphology results revealed that the fabric's surface was utterly coated by the nanoparticles leading to the formation of a highly packed nano-scale structure in the case of superhydrophobic coating. X-ray photoelect...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Raeisi M,Kazerouni Y,Mohammadi A,Hashemi M,Hejazi I,Seyfi J,Khonakdar HA,Davachi SM

    更新日期:2021-01-06 00:00:00

  • [HNMP]HSO4 catalyzed synthesis of selenized polysaccharide and its immunomodulatory effect on RAW264.7 cells via MAPKs pathway.

    abstract::In this paper, selenized Artemisia sphaerocephala polysaccharides (SePAS) were obtained through employing N-methyl-2-pyrrolidone hydrosulfate as catalyst, which showed a maximum Se content enhanced to 8744 μg/g. FT-IR, 1D/2D NMR, X-ray photoelectron spectroscopy (XPS) and size-exclusion chromatograph analysis exhibite...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Li R,Qin X,Liu S,Zhang X,Zeng X,Guo H,Wang T,Zhang Y,Zhang J,Zhang J,Wang J

    更新日期:2020-10-01 00:00:00

  • Effect of hydroxypropyl-β-cyclodextrin on the bounding of salazosulfapyridine to human serum albumin.

    abstract::The supramolecular interaction between salazosulfapyridine (SASP) and hydroxypropyl-β-cyclodextrin (HP-β-CD), as well as the influence of HP-β-CD on SASP's binding to human serum albumin (HSA), were investigated. Phase-solubility studies indicate that the HP-β-CD/SASP inclusion complex was formed at a 1:1 host-guest s...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Tang P,Tang B,Wang Q,Xu K,Xiong X,Li H

    更新日期:2016-11-01 00:00:00

  • Isolation and structural characterization of a novel polysaccharide from Hericium erinaceus fruiting bodies and its arrest of cell cycle at S-phage in colon cancer cells.

    abstract::The fruiting body of Hericium erinaceus has been used to treat digestive system disorder-related diseases for over 2000 years in China. A novel polysaccharide, HEFP-2b, was obtained from H. erinaceus fruiting bodies. Physical and chemical analysis showed that HEFP-2b consisted of fucose, galactose, glucose, and mannos...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Liu JY,Hou XX,Li ZY,Shan SH,Chang MC,Feng CP,Wei Y

    更新日期:2020-08-15 00:00:00

  • Endogenous calcium attenuates the immunomodulatory activity of a polysaccharide from Lycium barbarum L. leaves by altering the global molecular conformation.

    abstract::A heteropolysaccharide, LP5, was purified from Lycium barbarum L. leaves. It was identified as a calcium-rich polysaccharide (8.6 mg calcium/g) with a molecular weight of 2.50 × 105 Da. The polysaccharide was composed of six kinds of monosaccharides, of which mannose and xylose are the main components. And it contains...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Zhang B,Wang M,Wang C,Yu T,Wu Q,Li Y,Lv Z,Fan J,Wang L,Zhang B

    更新日期:2019-02-15 00:00:00

  • Exploration of the polar microenvironment around the reactive cysteine in rabbit muscle creatine kinase.

    abstract::The polar microenvironment around the reactive Cys283 of rabbit muscle creatine kinase was explored using kinetic analysis of substrates reaction in the presence of modifiers. In the present study, three specific sulphydryl reagents, 5,5'-dithiobis(2-nitrobenzoic acid), 6,6'-dithiodinicotinic acid and 2,2'-dithiodipyr...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: He HW,Li J,Zhao TJ,Ma Y,Shi F,Zhou HM

    更新日期:2007-10-01 00:00:00