Abstract:
:Isomaltulose production by bacterial fermentation was limited, due to generation of undesirable products and reduced yields. Isomaltulose production using sucrose isomerase (SIase) catalyzed methods was expected to be more applicable, but was hampered by low SIase activity and lack of a secreted SIase producer. Here, we aimed to obtain high levels of secreted SIase by overexpressing the SIase gene from Pantoea dispersa UQ68J in Yarrowia lipolytica, a successful host for efficient secretory expression, with a newly characterized strong constitutive promoter. After optimization of the culture medium, the engineered strain JD secreted SIase with an activity of 49.3 U/mL. The recombinant SIase was effectively immobilized onto polyvinyl alcohol-alginate, and the enzymatic activity recovery rate was up to 82.4%. The stability of the SIase was significantly improved by immobilization. Batch production of isomaltulose catalyzed by the immobilized SIase was performed under optimal conditions, generating 620.7 g/L isomaltulose with a yield of 0.96 g/g. The conversion rate of sucrose after 13 batches remained above 90%. These results demonstrated that the proposed SIase expression and immobilization method was promising in the industrial production of isomaltulose.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Zhang P,Wang ZP,Liu S,Wang YL,Zhang ZF,Liu XM,Du YM,Yuan XLdoi
10.1016/j.ijbiomac.2018.10.010subject
Has Abstractpub_date
2019-01-01 00:00:00pages
97-103eissn
0141-8130issn
1879-0003pii
S0141-8130(18)33573-6journal_volume
121pub_type
杂志文章abstract::The synthesis and characterization of a novel biodegradable graft copolymer based on partially hydrolyzed polymethylacrylate (PMA) grafted amylopectin (AP) was reported which was developed for the synthesis of silver nanoparticles from silver nitrate solution by facile green technique. The prepared graft copolymer was...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.07.046
更新日期:2015-11-01 00:00:00
abstract::Here, we aims to demonstrate a simple concept in biomaterials design by using natural resources solely as raw materials to fabricate elastic wound care dressing. Elasto-gel films comprise of silk sericin (SRC), natural rubber (NR), and chitin whisker (CTW) were developed. A glue-like protein SRC found in silk cocoons ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.03.094
更新日期:2017-08-01 00:00:00
abstract::The present study describes a novel ecofriendly series of chitin/curcumin/1,4-butane diol (BDO) blend derived polyurethanes (PUs), using hydroxy terminated polybutadiene (HTPB) and hexamethylene diisocyanate (HDI) along with different mole ratio of chitin, curcumin and BDO. The structural and morphological elucidation...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.07.149
更新日期:2017-12-01 00:00:00
abstract::Reduced graphene oxide (RGO) has shown tremendous potential as a NIR responsive nanomaterial and has been extensively explored for NIR mediated photothermal therapy and drug delivery. However, the potential of NIR as a stimulus to trigger release of entrapped/complexed DNA from its surface have not been explored. Stro...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.02.216
更新日期:2020-06-15 00:00:00
abstract::Pullulan (PU) is an exo-polysaccharide used in the food and pharmaceutical industries. However, the use of PU in different industries is limited due to its highly hydrophilic nature and consequently weakness in surface properties which can be remedied by its chemical modification with octenyl succinic anhydride (OSA)....
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.158
更新日期:2020-12-01 00:00:00
abstract::The exopolysaccharides from Arthrobacter sp. B4 (B4-EPS) exhibited an excellent chromium (VI) (Cr(VI)) removal capability without any pH adjustment, whereby 50mgL(-1) of Cr(VI) could be completely removed by 4gL(-1) of B4-EPS. The kinetics tests revealed that the first-order rate constant was 8.3×10(-5)s(-1) and the o...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.07.013
更新日期:2015-11-01 00:00:00
abstract::The neurotoxicity of HIV-1 Tat protein contributes significantly to the pathogenesis of HAND, and hence the attractive therapeutic strategies focusing on Tat-induced neurotoxicity are warranted. Salvia miltiorrhiza have been known to antioxidant property and neuroprotective effects. The Danshen granule is the pharmace...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.11.146
更新日期:2019-03-01 00:00:00
abstract::Several pectin derivatives were prepared by chemical modifications of the polysaccharide with natural fatty acids. The obtained biodegradable pectin-based materials, pectin-linoleate, pectin-oleate and pectin-palmitate, were investigated for their antimicrobial activity against several bacterial strains, Staphylococcu...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2014.04.011
更新日期:2014-07-01 00:00:00
abstract::This paper describes the use of a protocol for conformational analysis of oligosaccharide structures related to the cell-wall polysaccharide of Streptococcus group A. The polysaccharide features a branched structure with an L-rhamnopyranose (Rhap) backbone consisting of alternating alpha-(1-->2) and alpha-(1-->3) link...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/0141-8130(95)92678-j
更新日期:1995-06-01 00:00:00
abstract::Biopolymers are widely used for the removal of chromium from aqueous medium but it possesses limitations like poor sorption capacity and low stability. To overcome the limitations of biopolymers and to improve their properties, the present study was designed in such a way to develop a novel sorbent with enhanced chrom...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.10.010
更新日期:2016-02-01 00:00:00
abstract::Chronotherapeutically programmed hydroxyethylcellulose (HEC) based compression coated doxazosin tablets were prepared and the influence of disintegrants croscarmellose sodium, L-hydroxypropylcellulose (L-HPC), gellan gum on drug release and in vivo performance were investigated. Infrared spectroscopy and differential ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2014.08.028
更新日期:2015-01-01 00:00:00
abstract::Clinical use of camptothecin (CPT) is hindered due to its poor water and oil solubility, active lactone ring instability and non-targeted toxicity. Recently we reported formulation of camptothecin microemulsions with increased solubility for the improved treatment of breast cancer. In this research chitosan stabilized...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.05.127
更新日期:2017-11-01 00:00:00
abstract::Chitin and chitosan are amino polysaccharides having massive structural propensities to produce bioactive materials with innovative properties, functions and diverse applications particularly in biomedical field. The specific physico-chemical, mechanical, biological and degradation properties offer efficient way to bl...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/j.ijbiomac.2016.02.004
更新日期:2016-05-01 00:00:00
abstract::Chitosan was chemically modified through a four-step procedure. First, the amino groups of chitosan have reacted with benzaldehyde (Derivative 1); second, hydroxyl groups on C6 of Derivative 1 have reacted with epichlorohydrin (Derivative 2); third, the epoxy groups of Derivative 2 have reacted with 4-aminosalicylic a...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.01.298
更新日期:2020-05-15 00:00:00
abstract::Glycerol has emerged as a cheap waste material due to blooming biodiesel manufacturing units worldwide. The need is to exploit the crude glycerol (CG) to produce useful products such as polyhydroxyalkanoate (PHA). Bacillus thuringiensis EGU45 was found to produce 1.5-3.5 gP HA L(-1) from feed containing 1-10% CG (vv(-...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.03.046
更新日期:2015-01-01 00:00:00
abstract::Our previous report showed that the novel polysaccharide SAP isolated from the fruiting bodies of Sarcodon aspratus induced Hela cells apoptosis via mitochondrial dysfunction. In this study we found that SAP enhanced immunostimulatory activities of RAW264.7 cells, which was characterized by increased the production of...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.08.147
更新日期:2018-12-01 00:00:00
abstract::The present study addresses the immobilization of the GOX enzyme into a conventional polyvinyl alcohol (PVOH) membrane and a multi-layer membrane system (PCL/PVOH/PCL), both synthetized through electrospinning technique. More specifically, the enzyme was encapsulated inside a PVOH hydrophilic membrane, which was in tu...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.06.217
更新日期:2020-11-01 00:00:00
abstract::Insect mitochondrial DNA (mtDNA) ranges from 14 to 19 kbp, and the size difference is attributed to the AT-rich control region. Jewel wasps have a parasitoid lifestyle, which may affect mitochondria function and evolution. We sequenced, assembled, and annotated mitochondrial genomes in Nasonia and outgroup species. Ge...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.11.007
更新日期:2021-01-01 00:00:00
abstract::The water-soluble crude polysaccharides, extracted from the rhizome of Smilax glabra, were fractionated using an anion exchange chromatography, yielding two fractions, F1 and F2. The crude and fractions (F1 and F2) mainly consisted of carbohydrates (66.7%-91.1%), proteins (7.30%-23.9%) and minor amount of sulfates (1....
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.09.138
更新日期:2018-12-01 00:00:00
abstract::Genipin is a fully assessed non-cytotoxic crosslinking compound. The chitosan|genipin physical properties such as morphology, roughness, porosity, hydrophilicity, ζ-potential, surface area and surface energy exert control over cell adhesion, migration, phenotype maintenance and intracellular signaling in vitro, and ce...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
doi:10.1016/j.ijbiomac.2016.03.075
更新日期:2016-12-01 00:00:00
abstract::Salecan, a novel water-soluble extracellular β-glucan, is very suitable for the hydrogel preparation. Here, pH-sensitive magnetic composite hydrogel was fabricated by the graft copolymerization of crotonic acid (CA) and N-(hydroxymethyl) acrylamide (HMAA) onto salecan in the presence of Fe3O4@SiO2 nanoparticles for do...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.10.043
更新日期:2018-02-01 00:00:00
abstract::We isolated and purified polysaccharide from the Camellia oleifera Abel. seed cake (CCP) and studied its hypoglycemic activity in vitro. The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8. The CCP contained conjuga...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.04.054
更新日期:2018-08-01 00:00:00
abstract::Bacterial cellulose (BC) has emerged as a biomaterial for diverse biomedical applications owing to its unique structural, physico-chemical, mechanical, and biological features. Its porous geometry and three-dimensional fibrous structure allow the impregnation of various materials into its matrix. The current study was...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.07.050
更新日期:2019-09-15 00:00:00
abstract::Chitosan-based hydrogel nanoparticles provide a higher brain concentration of methotrexate (MTX) following IV administration in comparison with the drug's simple solution. The present study investigates the mechanism of this phenomenon, focusing on the possible role of P-gp. A previously developed MTX containing chito...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.12.093
更新日期:2019-03-15 00:00:00
abstract::Normal corn starch was treated with acidic ethanol solution with various concentrations of ethanol (0, 10, 30, 50 and 95%) and acid (0.0026, 0.0053 and 0.0079M) and then subjected to a dry heating treatment for 1, 2 and 4h at 130°C to prepare starch nanoparticles. Size of nanoparticles was determined using DLS (dynami...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.11.072
更新日期:2019-02-15 00:00:00
abstract::A novel thermostable xylanase gene from Chaetomium sp. CQ31 was cloned and codon-optimized (CsXynBop). The deduced protein sequence of the gene shared the highest similarity of 75% with the glycoside hydrolase (GH) family 10 xylanase from Achaetomium sp. Xz-8. CsXynBop was over-expressed in Pichia pastoris GS115 by hi...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.12.040
更新日期:2021-01-31 00:00:00
abstract::The present study was performed to investigate the chemical composition and antioxidant activity of glycoprotein purified from Undaria pinnatifida Harvey (UPGP). On SDS-PAGE, UPGP migrated as a single band with a molecular weight of approximately 10 kDa and confirmed by staining with Schiff's reagent as glycoprotein. ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2013.09.009
更新日期:2013-11-01 00:00:00
abstract::Starch retrogradation during freeze drying is a contributor to an undesirable decrease in the viscosity of soup prepared from instant soup with high starch content. Monitoring starch retrogradation during drying is therefore of importance if such a process is to be optimized. Here, cream mushroom soup was used as an e...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.01.084
更新日期:2020-03-15 00:00:00
abstract::In this paper, the electronspun Polylactic acid (PLA)/TiO2 nanofluids (nfs) fibrous membrane with good toughness, hydrophilicity and antibacterial activities are fabricated by taking full advantages of solvent-free TiO2 nfs with amphiphilicity and ionic conductivity. The resulting PLA/TiO2 nfs fibrous membrane exhibit...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.06.027
更新日期:2020-10-15 00:00:00
abstract::The phenomenon of polymer adsorption was applied to modify the surface of microcrystalline cellulose (MCC). The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs. The average particle size of unmodified MCC was about 14.208 ± 0.064 μm, and it was...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.04.051
更新日期:2019-07-01 00:00:00