Overexpression of secreted sucrose isomerase in Yarrowia lipolytica and its application in isomaltulose production after immobilization.

Abstract:

:Isomaltulose production by bacterial fermentation was limited, due to generation of undesirable products and reduced yields. Isomaltulose production using sucrose isomerase (SIase) catalyzed methods was expected to be more applicable, but was hampered by low SIase activity and lack of a secreted SIase producer. Here, we aimed to obtain high levels of secreted SIase by overexpressing the SIase gene from Pantoea dispersa UQ68J in Yarrowia lipolytica, a successful host for efficient secretory expression, with a newly characterized strong constitutive promoter. After optimization of the culture medium, the engineered strain JD secreted SIase with an activity of 49.3 U/mL. The recombinant SIase was effectively immobilized onto polyvinyl alcohol-alginate, and the enzymatic activity recovery rate was up to 82.4%. The stability of the SIase was significantly improved by immobilization. Batch production of isomaltulose catalyzed by the immobilized SIase was performed under optimal conditions, generating 620.7 g/L isomaltulose with a yield of 0.96 g/g. The conversion rate of sucrose after 13 batches remained above 90%. These results demonstrated that the proposed SIase expression and immobilization method was promising in the industrial production of isomaltulose.

journal_name

Int J Biol Macromol

authors

Zhang P,Wang ZP,Liu S,Wang YL,Zhang ZF,Liu XM,Du YM,Yuan XL

doi

10.1016/j.ijbiomac.2018.10.010

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

97-103

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(18)33573-6

journal_volume

121

pub_type

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