Production and physicochemical characterization of bacterial poly gamma- (glutamic acid) to investigate its performance on enhanced oil recovery.

Abstract:

:Bacillus licheniformis LMG 7559, which is capable of producing extracellular poly gamma- (glutamic acid) (PGA), was provided for the biopolymer synthesis. Using a modified PGA medium for PGA production, the isolated biopolymer, undergone dialysis process mainly for desalination and removal of other impurities. The bacteria produced high molecular weight biopolymers with a weight average molecular weight (M̅n) of 1.6 × 105 g/mole identified by gel permeation chromatography (GPC). Furthermore, GPC analysis was utilized to determine the poly-dispersity of PGA as well as molecular weight variation by cultivation time. The heavy weight fraction of 1.85 × 105 g/mole with poly-dispersity index of 7.42 was distinguished. For the extracted and dialyzed biopolymer, thermal properties were studied using DSC/TGA by which a mass loss of 36 percent was observed. Eventually, the biopolymer solution was injected into the oil saturated heterogeneous porous medium to evaluate the recovery factor enhancement by PGA flooding. It was found that 31.45% of oil in place was recovered by biopolymer flooding, whereas only 16.6% of oil in place was obtained by water flooding.

journal_name

Int J Biol Macromol

authors

Azarhava H,Bajestani MI,Jafari A,Vakilchap F,Mousavi SM

doi

10.1016/j.ijbiomac.2019.10.090

subject

Has Abstract

pub_date

2020-03-15 00:00:00

pages

1204-1212

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(19)35743-5

journal_volume

147

pub_type

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