Abstract:
:Bacillus licheniformis LMG 7559, which is capable of producing extracellular poly gamma- (glutamic acid) (PGA), was provided for the biopolymer synthesis. Using a modified PGA medium for PGA production, the isolated biopolymer, undergone dialysis process mainly for desalination and removal of other impurities. The bacteria produced high molecular weight biopolymers with a weight average molecular weight (M̅n) of 1.6 × 105 g/mole identified by gel permeation chromatography (GPC). Furthermore, GPC analysis was utilized to determine the poly-dispersity of PGA as well as molecular weight variation by cultivation time. The heavy weight fraction of 1.85 × 105 g/mole with poly-dispersity index of 7.42 was distinguished. For the extracted and dialyzed biopolymer, thermal properties were studied using DSC/TGA by which a mass loss of 36 percent was observed. Eventually, the biopolymer solution was injected into the oil saturated heterogeneous porous medium to evaluate the recovery factor enhancement by PGA flooding. It was found that 31.45% of oil in place was recovered by biopolymer flooding, whereas only 16.6% of oil in place was obtained by water flooding.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Azarhava H,Bajestani MI,Jafari A,Vakilchap F,Mousavi SMdoi
10.1016/j.ijbiomac.2019.10.090subject
Has Abstractpub_date
2020-03-15 00:00:00pages
1204-1212eissn
0141-8130issn
1879-0003pii
S0141-8130(19)35743-5journal_volume
147pub_type
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