当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Isolation, cloning and characterization of an azoreductase and the effect of salinity on its expression in a halophilic bacterium.

Isolation, cloning and characterization of an azoreductase and the effect of salinity on its expression in a halophilic bacterium.

Abstract：

:Understanding the molecular mechanisms of azo dye decolorization is important for the development of effective bioremediation for textile-colored wastewater. A halophilic bacterium Halomonas sp. strain GT was isolated, which could degrade the azo dye Acid Brilliant Scarlet GR at 10% NaCl. The complete genome sequence of this strain was obtained using the PacBio RS II platform. Genome annotation revealed that four proteins are related to decolorization of azo dyes, such as azoreductase, laccases, benzene 1,2-dioxygenase, and catechol 1,2-dioxygenase. The putative azoreductase gene of Halomonas sp. strain GT responsible for the decolorization of azo dye in high salt environment was isolated. Phylogenetic tree analysis showed that the azoG (azoreductase gene of Halomonas sp. strain GT) and its homologs constituted a new branch of the NADH depending azoreductases, with all the homologous sequence of the protein from halophilic bacteria. At high NaCl concentrations, azoreductase gene expression and azoreductase activity were restrained in Halomonas sp. strain GT, which resulted in low a decolorization rate.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Tian F,Guo G,Zhang C,Yang F,Hu Z,Liu C,Wang SW

doi

10.1016/j.ijbiomac.2018.11.175

subject

Has Abstract

pub_date

2019-02-15 00:00:00

pages

1062-1069

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(18)35173-0

journal_volume

123

pub_type

杂志文章

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