Abstract：
:The use of helminth infections as tools to understand the type 2 immune response is a well-established technique and important to many areas of immunological research. The phenotype and function of immune cell populations at the site of infection is a key determinant of pathogen clearance. However, infections with helminths such as the murine nematode Heligomosmoides polygryrus cause increased mucus production and thickening of the intestinal wall, which can result in extensive cell death when isolating and analysing cells from the lamina propria (LP). Populations of larger immune cells such as macrophages and dendritic cells are often trapped within mucus or dying tissues. Here we describe an optimised protocol for isolating LP leukocytes from the small intestine of H.polygyrus -infected mice, and we demonstrate phenotypic and functional identification of myeloid and CD4+ T cell subsets using cytokine staining and flow cytometry. Our protocol may provide a useful experimental method for the immunological analysis of the affected tissue site during helminth infections.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Webster HC,Andrusaite AT,Shergold AL,Milling SWF,Perona-Wright Gdoi
10.1016/j.jim.2019.112702subject
Has Abstractpub_date
2020-02-01 00:00:00pages
112702eissn
0022-1759issn
1872-7905pii
S0022-1759(19)30352-7journal_volume
477pub_type
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journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
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pub_type： 杂志文章
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journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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更新日期：2004-01-01 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/0022-1759(85)90488-0
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journal_title：Journal of immunological methods
pub_type： 杂志文章
doi：10.1016/0022-1759(90)90337-u
更新日期：1990-02-20 00:00:00
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journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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journal_title：Journal of immunological methods
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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更新日期：1995-12-01 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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更新日期：1983-05-13 00:00:00
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journal_title：Journal of immunological methods
pub_type： 杂志文章
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更新日期：1990-02-20 00:00:00
