Abstract：
:alpha-2-macroglobulin (alpha-2-M), a plasma proteinase inhibitor, plays an important role in the pathogenesis of lung inflammation. The purpose of this study was to develop a sensitive ELISA assay for rabbit alpha-2-M to allow us to define the role of this protein in a rabbit model of lung inflammation/injury. Therefore, we developed hybridomas which secrete monoclonal antibodies (mAbs) against rabbit alpha-2-M. From the antibodies produced, two (5B6 and 3C5) were selected. Both of them were of the IgG1 subclass. 5B6 reacted with native alpha-2-M as well as with the "fast" form of alpha-2-M (native alpha-2-M or the "slow" form is converted to the "fast" form by reaction with a proteinase). On the other hand, 3C5 reacted only with "fast" form of alpha-2-M. With these antibodies, we developed two ELISA assays which were used to determine the concentration of alpha-2-M in the lung fluids from rabbits with lung injury.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Kurdowska A,Miller EJ,Krupa A,Noble JM,Sakao Ydoi
10.1016/s0022-1759(02)00278-8keywords:
subject
Has Abstractpub_date
2002-12-15 00:00:00pages
147-53issue
2eissn
0022-1759issn
1872-7905pii
S0022175902002788journal_volume
270pub_type
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