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Isolating pure populations of monocytes from the blood of pregnant women: comparison of flotation in iodixanol with elutriation.

Abstract：

:Observations that the innate arm of the immune system is upregulated in pregnancy have highlighted the need for methods of isolating pure populations of monocytes for studies into pregnancy and pre-eclampsia without activating them during the isolation process. Density gradient centrifugation using iodixanol is a useful method for isolating relatively pure populations of unactivated monocytes from human blood but has not been validated in pregnant subjects. We compared the ability of monocytes isolated from pregnant women by density gradient centrifugation using iodixanol (n=6) with monocytes isolated by countercurrent centrifugal elutriation (n=6) in terms of their ability to produce interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) under basal conditions and after stimulation with bacterial lipopolysaccharide (LPS). Under basal conditions, monocytes isolated by density gradient centrifugation produced low amounts of IL-6 and MCP-1. Production of IL-6 and MCP-1 after stimulation of the monocytes with LPS was much greater (p<0.01). There was no statistically significant difference between the two methods in terms of stimulated levels of either cytokine.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Nutt JC,Willis CC,Morris JM,Gallery ED

doi

10.1016/j.jim.2004.05.011

keywords:

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

215-8

issue

1-2

eissn

0022-1759

issn

1872-7905

pii

S0022-1759(04)00278-9

journal_volume

293

pub_type

杂志文章

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