Abstract:
:This paper describes a method to enhance the sensitivity of enzyme immunoassays by the use of solid tagged latex beads. Various bead sizes (0.21 and 0.81 micron) were tested using particles which had been covalently modified (or coated) with biotinylated rabbit anti-mouse immunoglobulin antibodies. Detection limits of mouse monoclonal antibodies or antigens (which have been reacted with specific mouse antibodies) were compared to those obtained using the general homogeneous 'sandwich' ELISA assay. Compared to the homogeneous biotin-avidin ELISA an increase in sensitivity level of about 6-10 times was observed in antigen detection assays. The stability of the reagent was excellent. The method gave low backgrounds and was as simple to use as the standard procedure.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Nilsson Bdoi
10.1016/0022-1759(88)90158-5subject
Has Abstractpub_date
1988-11-10 00:00:00pages
89-93issue
1-2eissn
0022-1759issn
1872-7905pii
0022-1759(88)90158-5journal_volume
114pub_type
杂志文章abstract::In an attempt to overcome the limitations of counterimmunoelectrophoresis in the detection of precipitating anti-DNA antibodies or circulating DNA, ethidium bromide has been used to increase the visibility of the precipitin lines and to confirm their specificity. ...
journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1985-11-07 00:00:00
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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更新日期:1993-01-14 00:00:00
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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