The demonstration of cell surface antigens on T cells, B cells and accessory cells in paraffin-embedded human tissues.

abstract：:A method for synthesising omega-stearoyldextran for the detection of plaque forming cells specific for the alpha-(1 replaces 6) determinant is described. The omega-stearoyl derivative is obtained by reacting dextran with phenylboronic acid to give a 2,4-phenylboronate, which can be subsequently esterified in a pyridin...

journal_title：Journal of immunological methods

authors： Svensson S,Vicari G,Wilkinson S

更新日期：1976-01-01 00:00:00

abstract：:Ethanol-fixed paraffin-embedded specimens of human tissues were studied whether the surface antigens of leukocytes in these tissues can be stained and analyzed. Three-layer indirect immunoperoxidase staining was performed on the ethanol-fixed paraffin-embedded sections by the use of several monoclonal antibodies for w...

journal_title：Journal of immunological methods

authors： Ootaka T,Saito T,Soma J,Yoshinaga K

更新日期：1990-08-07 00:00:00

abstract：:We describe here the use of a new agarose for isoelectric focusing of body fluids and tissue proteins. Macromolecular proteins even when significantly greater in size than 1 X 10(6) mol.wt. were easily and rapidly focused. ...

journal_title：Journal of immunological methods

authors： Saravis CA,Zamcheck N

更新日期：1979-01-01 00:00:00

abstract：:The amino-acid sequence of the very high-affinity anti-angiotensin II monoclonal antibody 4D8 was predicted from the nucleotide sequence of the heavy and light chain variable genes. The single-chain variable fragment (scFv) was constructed and expressed in Escherichia coli as a soluble protein and at the surface of th...

journal_title：Journal of immunological methods

authors： Cohen P,Laune D,Teulon I,Combes T,Pugnière M,Badouaille G,Granier C,Mani JC,Simon D

更新日期：2001-08-01 00:00:00

abstract：:C3 nephritic factor (C3NeF) is an autoantibody against the C3 convertase which stabilizes this otherwise inherently labile neoenzyme and induces a continuous activation of the alternative pathway with C3 depletion. NeF is found in patients with membranoproliferative glomerulonephritis and/or partial lipodystrpohy. NeF...

journal_title：Journal of immunological methods

authors： Jelezarova E,Schlumberger M,Sadallah S,Späth PJ,Schifferli JA,Lutz HU

更新日期：2001-05-01 00:00:00

abstract：:Melanins are notoriously difficult to work with because of their unique physical and chemical properties. The study of melanins is hampered by the scarcity of melanin-specific reagents and serological techniques. In this study we describe modifications to the standard method for the isolation of melanins from in vitro...

journal_title：Journal of immunological methods

authors： Rosas AL,Nosanchuk JD,Gómez BL,Edens WA,Henson JM,Casadevall A

更新日期：2000-10-20 00:00:00

abstract：:Dilatometry has become a useful method for the study of proteins owing to its simplicity and accuracy. However, it has seldom been used in the study of immunoglobulins. Therefore possible applications of the method for that study are being discussed. A description is also given of the most common experimental set-up a...

journal_title：Journal of immunological methods

authors： Lapanje S,Skerjanc J

更新日期：1975-12-01 00:00:00

abstract：:Neisseria meningitidis is a leading cause of morbidity and mortality worldwide. The presently available capsular polysaccharide vaccine is poorly immunogenic in children under the age of 2 due to its T-independent (TI) nature. Efforts to overcome the TI response elicited by the polysaccharide vaccine have led to the d...

journal_title：Journal of immunological methods

authors： Prinz DM,Smithson SL,Westerink MA

更新日期：2004-02-01 00:00:00

abstract：:The major isoform of hGH is a polypeptide of 191 amino acids. Human GH1-43 is an amino terminal segment of hGH1-191 which comprises the first 43 amino acids. This peptide is a potent regulator of glucose homeostasis. To facilitate our understanding of the physiological regulation of hGH1-43 an assay to measure its lev...

journal_title：Journal of immunological methods

authors： López-Guajardo CC,Armstrong LS,Jordan L,Staten NR,Krivi GG,Martinez AO,Haro LS

更新日期：1998-06-01 00:00:00

abstract：:Direct application of membrane fractions to a nitrocellulose support without previous solubilization, denaturation or fixation of the antigen permits the quantitation of antibodies binding to denaturation sensitive epitopes. In experiments reported here, this method was used to determine the rate of EGF receptor expre...

journal_title：Journal of immunological methods

authors： Von Knebel Doeberitz M,Drzonek H,Koch S,Becker CM

更新日期：1989-09-01 00:00:00

abstract：:In studies of the relation between structure and function of proteins of the immune system, there is a continuous need for screening of a large number of protein variants. To optimise the yield following transient gene expression in small or medium culture volumes, several parameters were investigated. First, secretio...

journal_title：Journal of immunological methods

authors： Berntzen G,Lunde E,Flobakk M,Andersen JT,Lauvrak V,Sandlie I

更新日期：2005-03-01 00:00:00

abstract：:A microELISA for the estimation of human serum thymosin alpha 1 is described. In this assay, antibody to thymosin alpha 1 is pre-incubated with the standard or serum at 4 degrees C. Unbound antibody in the liquid-phase then binds with solid-phase thymosin alpha 1. The method is sufficiently sensitive for measuring ser...

journal_title：Journal of immunological methods

authors： Weller FE,Mutchnick MG,Keren DF,Goldstein AL,Naylor PH

更新日期：1985-06-12 00:00:00

abstract：:Concanavalin A-activated murine suppressor T cells act via the mediator, soluble immune response suppressor (SIRS) which non-specifically suppresses IgM and IgG antibody responses to a variety of antigens, cytotoxic T lymphocyte responses and proliferative responses to alloantigens and mitogens in vitro. SIRS is a pro...

journal_title：Journal of immunological methods

authors： Aune TM,Pierce CW

更新日期：1982-08-27 00:00:00

abstract：:The sensitivity and specificity of immunohistological staining procedures employing horseadish peroxidase labeled antibody were evaluated with the aid of chessboard titrations. Two indirect staining systems, detecting antinuclear antibody and pemphigus intercellular antibody, were examined with reference to indirect i...

journal_title：Journal of immunological methods

authors： Shu S,Albini B

更新日期：1976-01-01 00:00:00

abstract：:A new T(H1)/T(H2) in vitro model for mechanistic studies and drug screening in human T cells was established working with ficoll-separated PBMCs or elutriated lymphocytes cultured in serum-free medium. Human T cells could be kept viable and reactive in this medium for several months. In this model, superantigens (SAs)...

journal_title：Journal of immunological methods

authors： Gehring S,Schlaak M,van der Bosch J

更新日期：1998-10-01 00:00:00

abstract：:The measurement of the Epstein-Barr viral load in peripheral blood has been recognised as an important way of monitoring the response to treatment in patients with Epstein-Barr virus (EBV)-related malignancies. In particular, EBV load in transplant recipients can be used as a predictive parameter for Post-transplant L...

journal_title：Journal of immunological methods

authors： Leung E,Shenton BK,Jackson G,Gould FK,Yap C,Talbot D

更新日期：2002-12-15 00:00:00

abstract：:The direct propagation of newly formed human hybridomas in serum-free medium selects for hybrids with a metabolism best suited to growth in this environment. Under optimal culture conditions, this procedure results in the generation of antigen-specific human hybridomas comparable in frequency, stability, and antibody ...

journal_title：Journal of immunological methods

authors： Lang AB,Schürch U,Zimmermann F,Bruderer U

更新日期：1992-09-18 00:00:00

abstract：:To compare the responses of individual neutrophils to chemoattractants, migration pathway data were obtained using TAXIScan, an optically accessible/horizontal apparatus in which a concentration gradient is established reproducibly for a given stimulus. The observed linear-mode trajectory pattern of neutrophils toward...

journal_title：Journal of immunological methods

authors： Yamauchi A,Degawa-Yamauchi M,Kuribayashi F,Kanegasaki S,Tsuchiya T

更新日期：2014-02-01 00:00:00

abstract：:IgM antibodies are often of low affinity (dissociation constant (Kd) > 10(-5) M) and therefore they are usually neglected as tools in, e.g., immunoassays. Previous studies have shown that low affinity biological interactions can be studied and exploited in affinity chromatography, biosensor technology and capillary el...

journal_title：Journal of immunological methods

authors： Strandh M,Ohlin M,Borrebaeck CA,Ohlson S

更新日期：1998-05-01 00:00:00

abstract：:The length of the heavy chain complementarity-determining region two (HCDR2) of the unmutated anti-p-azophenylarsonate (Ars) monoclonal antibody (36-65 mAb) was extended by three residues in order to test whether this insertion can provide additional contacts between the Ab and the antigen. Two libraries were generate...

journal_title：Journal of immunological methods

authors： Parhami-Seren B,Viswanathan M,Margolies MN

更新日期：2002-01-01 00:00:00

abstract：:Testing of diphtheria vaccines for routine lot release relies heavily on the use of in vivo potency assays. However, consistency of production is also recognised as an important feature of vaccine manufacture, and in vitro assays are superior to in vivo assays for providing this information. In adsorbed vaccines, anti...

journal_title：Journal of immunological methods

authors： Coombes L,Stickings P,Tierney R,Rigsby P,Sesardic D

更新日期：2009-10-31 00:00:00

abstract：:In vitro antibody-dependent cell-mediated cytotoxicity (ADCC) assays are routinely performed to support the research and development of therapeutic antibodies. In ADCC assays, target cells bound by the antibodies are lysed by activated effector cells following interactions between the Fc region of the bound antibody a...

journal_title：Journal of immunological methods

authors： Chung S,Nguyen V,Lin YL,Kamen L,Song A

更新日期：2017-08-01 00:00:00

abstract：:Haemophilia A patients who receive repeated transfusion of fVIII concentrates often develop inhibitor alloantibodies, resulting in reduced efficacy of the therapy. Determination of fVIII epitopes for the alloantibodies is essential for an understanding of their inhibitory effect on blood coagulation. Random fragments ...

journal_title：Journal of immunological methods

authors： Kuwabara I,Maruyama H,Kamisue S,Shima M,Yoshioka A,Maruyama IN

更新日期：1999-04-22 00:00:00

abstract：:A sandwich amperometric enzyme immunoassay with flow injection for alpha-fetoprotein in human serum has been developed with alkaline phosphatase as the enzyme label. p-Hydroxyphenyl phosphate was used as the substrate for alkaline phosphatase. The hydrolysis product, hydroquinone, was detected by oxidative amperometry...

journal_title：Journal of immunological methods

authors： Della Ciana L,Bernacca G,De Nitti C,Massaglia A

更新日期：1996-06-14 00:00:00

abstract：:Helicobacter pylori infection is the most common human infection where approximately 50% of the world populations are infected. The diagnosis of such infection is mainly done by endoscopy where gastric biopsies are examined for the presence of H. pylori. Such invasive approach is costly, time consuming and generally r...

journal_title：Journal of immunological methods

authors： Karakus C,Salih BA

更新日期：2013-10-31 00:00:00

abstract：:Real-time polymerase chain reaction (PCR) is now becoming an accepted tool for measuring gene expression at the transcriptional level. In this study, a direct comparison between real-time PCR, enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunosorbent spot (ELISPOT) assay was performed. When interferon-...

journal_title：Journal of immunological methods

authors： Mena A,Ioannou XP,Van Kessel A,Van Drunen Little-Van Den Hurk S,Popowych Y,Babiuk LA,Godson DL

更新日期：2002-05-01 00:00:00

abstract：:A new antibody overlay technique for 2-dimensional immunoelectrophoresis (2-D-IEP) is described. After the first dimension electrophoresis of the antigen, the desired amount of antiserum is applied to the initial agarose layer and then evenly distributed over a defined surface of the gel with a PVC film. This modifica...

journal_title：Journal of immunological methods

authors： Lebecq JC,Salhi SL,Bastide JM

更新日期：1984-02-10 00:00:00

abstract：:The multiple antigen peptide (MAP) system has been proposed as a novel and valuable approach for eliciting antibodies to peptides and developing synthetic vaccines. The MAP system consists of a small immunogenically inert core matrix of lysine residues with alpha- and epsilon-amino groups for anchoring multiple copies...

journal_title：Journal of immunological methods

authors： Briand JP,Barin C,Van Regenmortel MH,Muller S

更新日期：1992-12-08 00:00:00

abstract：:The haem enzyme myeloperoxidase (MPO) (EC 1.11.1.7) with a spectral A430/A280 ratio greater than 0.7 and a specific activity of 125 U/mg was purified from isolated human neutrophils. To obtain a radioimmunoassay (RIA) for this enzyme, a specific antiserum against human neutrophil MPO was raised in rabbits and used at ...

journal_title：Journal of immunological methods

authors： Pincemail J,Deby-Dupont G,Deby C,Thirion A,Torpier G,Faymonville ME,Damas P,Tomassini M,Lamy M,Franchimont P

更新日期：1991-03-21 00:00:00

abstract：:Embryonic stem cells provide a potentially convenient source of macrophages in the laboratory. Given the propensity of macrophages for plasticity in phenotype and function, standardised culture and differentiation protocols are required to ensure consistency in population output and activity in functional assays. Here...

journal_title：Journal of immunological methods

authors： Zhuang L,Pound JD,Willems JJ,Taylor AH,Forrester LM,Gregory CD

更新日期：2012-11-30 00:00:00