Selective generation of antigen-specific human hybridomas optimized for large scale growth in serum-free medium.

abstract：:Plasma cells (PCs) secrete antibodies and play an essential role in protective immunity, but also in pathogenesis of antibody-mediated diseases. Physiologically, PCs mainly reside within bone marrow and spleen. In autoimmune diseases such as systemic lupus erythematosus (SLE) autoantibody-producing PCs can also be fou...

journal_title：Journal of immunological methods

authors： Schaffer S,Maul-Pavicic A,Voll RE,Chevalier N

更新日期：2019-11-01 00:00:00

abstract：:Phagocytosis plays a pivotal and essential role in host immune defense, both as a focal constituent of the innate immune response and a bridging element linking innate and adaptive immunity. Phagocytosis has been demonstrated to be critical in development, tissue remodeling, wound healing and resolution of inflammatio...

journal_title：Journal of immunological methods

authors： Neaga A,Lefor J,Lich KE,Liparoto SF,Xiao YQ

更新日期：2013-04-30 00:00:00

abstract：:A solid-phase enzyme immunoassay for the measurement of anti-acetylcholine receptor antibodies in the sera of patients with myasthenia gravis is reported. Sufficient amounts of acetylcholine receptor for the sensitive detection of anti-acetylcholine receptor antibody were directly fixed to Costar serocluster 96-well E...

journal_title：Journal of immunological methods

authors： Kobayashi N,Sugita H,Terada E,Ghoda A,Okudaira H,Ogita T,Miyamoto T

更新日期：1984-10-26 00:00:00

abstract：:Monoclonal antibodies have been prepared against a synthetic peptide with a sequence corresponding to a repeated hydrophilic region of the protein core of the human MUC-2 gastrointestinal mucin. Peptide conjugates, prepared by glutaraldehyde cross-linking with keyhole limpet haemocyanin (KLH) and bovine serum albumin ...

journal_title：Journal of immunological methods

authors： Price MR,Sekowski M,Hooi DS,Durrant LG,Hudecz F,Tendler SJ

更新日期：1993-02-26 00:00:00

abstract：:An assay for detection of monoclonal hybridoma antibodies against cell surface antigens is described. Samples of spent medium from the hybridoma cultures are incubated in microtest wells with cells, either as adherent monolayers or in suspension. Antibodies bound to surface antigens are detected by successive incubati...

journal_title：Journal of immunological methods

authors： Brown JP,Tamerius JD,Hellström I

更新日期：1979-01-01 00:00:00

abstract：:Many labs wish to measure cytokines in an accurate, reproducible, and rapid manner. An antibody-based membrane array for measuring cytokines has been developed based on the same technology as the traditional ELISA. The aim of this study was to compare results obtained with the traditional ELISA method with those from ...

journal_title：Journal of immunological methods

authors： Copeland S,Siddiqui J,Remick D

更新日期：2004-01-01 00:00:00

abstract：OBJECTIVE:The objective of this study was the identification of the stain HIF-alpha using the Image Cytometry, and to help to count the positive cells (with HIF-alpha) and the negative cells (without HIF-alpha) from the same sample. METHOD:17 images of renal tissues from male rats of Winstar lineage; overall, there we...

journal_title：Journal of immunological methods

authors： Buzin AR,Macedo ND,De Araujo IB,Nogueira BV,de Andrade TU,Endringer DC,Lenz D

更新日期：2017-05-01 00:00:00

abstract：:Picloram-specific variable fragments (V(HH)s) of heavy chain antibodies (HCAbs) were selected from a nai;ve-llama library using ribosome display technology. A cDNA library of V(HH)s was constructed from lymphocytes of a non-immunized llama and engineered to allow in vitro transcription and translation. With no stop co...

journal_title：Journal of immunological methods

authors： Yau KY,Groves MA,Li S,Sheedy C,Lee H,Tanha J,MacKenzie CR,Jermutus L,Hall JC

更新日期：2003-10-01 00:00:00

abstract：:A simple method to study 19S anti-SRBC antibody production is described. This technique, performed by incubation of immune spleen cells, SRBC and C in the fluid phase, utilises photomeric estimation of antibody mediated haemoglobin release. The test is quicker and more accurate than the two-step haemolytic plaque assa...

journal_title：Journal of immunological methods

authors： Van Dijk H,Bloksma N

更新日期：1977-01-01 00:00:00

abstract：:This paper describes a novel method for detecting immunoglobulin A (IgA) coated potentially pathogenic microorganisms (PPMs) in the human intestine. Essentially, the technique consists of 2 phases: one in which IgA coated bacteria are detected by immunofluorescence and a second in which these bacteria are subcultured ...

journal_title：Journal of immunological methods

authors： Van Saene HK,Van der Waaij D

更新日期：1979-01-01 00:00:00

abstract：:Dot-immunobinding assay for anti-calmodulin (CaM), and immunoblot assay for CaM have become feasible by proper choice of a fixative, i.e., formaldehyde vapor. The protocol for these simple and convenient immunological procedures is presented. Dot-immunobinding assay could be accomplished in 3 h, and immunoblot assay i...

journal_title：Journal of immunological methods

authors： Natsukari N,Ohta H,Fujita M

更新日期：1989-12-20 00:00:00

abstract：:The Immune Epitope Database and Analysis Resource (IEDB, http://www.iedb.org) hosts a continuously growing set of immune epitope data curated from the literature, as well as data submitted directly by experimental scientists. In addition, the IEDB hosts a collection of prediction tools for both MHC class I and II rest...

journal_title：Journal of immunological methods

authors： Kim Y,Sette A,Peters B

更新日期：2011-11-30 00:00:00

abstract：:Wegener's granulomatosis (WG) is a systemic vasculitis which is diagnosed on clinicopathological findings. The diagnosis may be aided by the presence of anti-neutrophil cytoplasm antibodies (ANCA). In WG, ANCA are primarily directed to proteinase 3 (PR3), a serine protease of the azurophilic granules of the neutrophil...

journal_title：Journal of immunological methods

authors： Baslund B,Petersen J,Permin H,Wiik A,Wieslander J

更新日期：1994-10-14 00:00:00

abstract：:C3 nephritic factor (C3NeF) is an autoantibody against the C3 convertase which stabilizes this otherwise inherently labile neoenzyme and induces a continuous activation of the alternative pathway with C3 depletion. NeF is found in patients with membranoproliferative glomerulonephritis and/or partial lipodystrpohy. NeF...

journal_title：Journal of immunological methods

authors： Jelezarova E,Schlumberger M,Sadallah S,Späth PJ,Schifferli JA,Lutz HU

更新日期：2001-05-01 00:00:00

abstract：:Helicobacter pylori infection is the most common human infection where approximately 50% of the world populations are infected. The diagnosis of such infection is mainly done by endoscopy where gastric biopsies are examined for the presence of H. pylori. Such invasive approach is costly, time consuming and generally r...

journal_title：Journal of immunological methods

authors： Karakus C,Salih BA

更新日期：2013-10-31 00:00:00

abstract：:Passage of human peripheral blood leucocytes through Sephadex G-10 columns results in a complete depletion of monocytes. Microscopic examination and ingestion of latex particles failed to reveal any monocytes in the column-passed cells. The study of surface markers shows a slight enrichment of T-cells and a decrease o...

journal_title：Journal of immunological methods

authors： Alonso CM,Bernabé RR,Moreno E,Diaz de Espada F

更新日期：1978-01-01 00:00:00

abstract：:Hybridomas secreting monoclonal antibodies specific for hemoglobin nonenzymatically glycated in the non-A1c position were produced by fusion of SP 2/0 myeloma cells with spleen cells from BALB/c mice immunized with nonenzymatically glycated hemoglobin prepared from human erythrocytes. Wells containing hydridomas secre...

journal_title：Journal of immunological methods

authors： Steward LA,Wu VY,Shea E,Cohen MP

更新日期：1991-07-05 00:00:00

abstract：:In this report we show that succinic groups are far more reactive to amino compounds than the carboxylic groups derived from Asp and Glu on the protein when using coupling via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDCI) (even by an 8 fold factor). Accordingly, a new carrier-protein was designed where both na...

journal_title：Journal of immunological methods

authors： Fuentes M,Palomo JM,Mateo C,Venteo A,Sanz A,Fernández-Lafuente R,Guisan JM

更新日期：2005-12-20 00:00:00

abstract：:Drug-specific monoclonal antibodies (MAbs) were produced against the very small drug hapten (162.15 Da), 5-benzimidazolecarboxylic acid, an analogue of 2-(4-Thiazolyl)benzimidazole (TBZ) but lacking the thiol group. TBZ is widely used as a broad-spectrum anthelmintic in various animal species and humans and also as a ...

journal_title：Journal of immunological methods

authors： Moran E,O'Keeffe M,O'Connor R,Larkin AM,Murphy P,Clynes M

更新日期：2002-12-20 00:00:00

abstract：:Here we extensively describe a FACS-based protocol for isolating intact non-stained human eosinophils from peripheral blood; a stop forward from our recently published initial study. This method of purification could be accomplished in <3 h with only small volumes of whole blood necessary, even in healthy subjects gen...

journal_title：Journal of immunological methods

authors： Wacht G,Poirot A,Charles AL,Radosavljevic M,Uring-Lambert B,de Blay F,Geny B,Bahram S,Barnig C

更新日期：2018-12-01 00:00:00

abstract：:Upon treatment with protein therapeutics, a subset of patients will typically develop antibodies against the drug. These anti-drug antibodies can be of concern because they have the potential to alter the drug's therapeutic activity. In the case of relapsing-remitting multiple sclerosis (RRMS) patients receiving recom...

journal_title：Journal of immunological methods

authors： Brickelmaier M,Hochman PS,Baciu R,Chao B,Cuervo JH,Whitty A

更新日期：1999-07-30 00:00:00

abstract：:IgM antibodies are often of low affinity (dissociation constant (Kd) > 10(-5) M) and therefore they are usually neglected as tools in, e.g., immunoassays. Previous studies have shown that low affinity biological interactions can be studied and exploited in affinity chromatography, biosensor technology and capillary el...

journal_title：Journal of immunological methods

authors： Strandh M,Ohlin M,Borrebaeck CA,Ohlson S

更新日期：1998-05-01 00:00:00

abstract：:An improved microassay for quantitation of murine macrophage-mediated antibody-dependent cellular cytotoxicity (ADCC) has been developed. The method is based on the turbidimetric measurement of sheep erythrocyte or nucleated (L1210) target cell suspensions at 630 nm with an automatic microtiter plate densitometer. The...

journal_title：Journal of immunological methods

authors： Rummage JA,Chang NS,Leu RW

更新日期：1986-01-22 00:00:00

abstract：:The possibility that immortalisation of in vitro immunised splenocytes may result in hybridomas secreting polyreactive antibodies was investigated. A panel of nine murine hybridomas, secreting IgM(kappa) anti-goat immunoglobulin G (anti-GIgG), was produced by immortalising splenocytes that had been immunised in vitro ...

journal_title：Journal of immunological methods

authors： McMahon MJ,O'Kennedy R

更新日期：2001-12-01 00:00:00

abstract：:A plasmid cassette vector was designed to generate transgenic mice expressing reporter cDNAs at high levels in antigen-presenting cells under the control of the murine invariant chain (Ii) promoter. Analysis of several transgenic mice harboring a chimeric Ii cDNA placed in this vector showed that it can drive expressi...

journal_title：Journal of immunological methods

authors： van Santen H,Benoist C,Mathis D

更新日期：2000-11-01 00:00:00

abstract：:Monoclonal antibodies (MAbs) were selected for specific binding to spent media of cultured tumor cells. Out of more than 12,000 hybridomas screened, 19 were selected in preliminary inhibition assays for secretion of MAbs which detected antigens in cancer patients' sera. Antibody CO 29.11, which was studied in detail, ...

journal_title：Journal of immunological methods

authors： Herlyn M,Blaszczyk M,Bennicelli J,Sears HF,Ernst C,Ross AH,Koprowski H

更新日期：1985-06-12 00:00:00

abstract：:We have developed an enzyme-linked immunosorbent assay (ELISA) for the quantitation of C4 nephritic factor (C4NeF). Incubation of the C4NeF-positive serum from patient M.I. with normal human serum (NHS) in the presence of human aggregated IgG (AHG) resulted in the formation of stable C4-C2 complex. No complex was form...

journal_title：Journal of immunological methods

authors： Seino J,Kinoshita Y,Sudo K,Horigome I,Sato H,Narita M,Noshiro H,Kudo K,Fukuda K,Saito T

更新日期：1990-03-27 00:00:00

abstract：:Highly sensitive and easy detection method for Alzheimer's disease (AD) with a suitable biomarker is mandatory for preventing the factors resulting from AD. This research reports a modified ELISA with graphene for the detection of AD biomarker amyloid beta (Aβ) oligomer. Gold nanoparticle (AuNP) conjugated aptamer was...

journal_title：Journal of immunological methods

authors： Zhao J,Chang W,Liu L,Xing X,Zhang C,Meng H,Gopinath SCB,Lakshmipriya T,Chen Y,Liu Y

更新日期：2021-02-01 00:00:00

abstract：:Precipitation with polyethylene glycol 6000 is a satisfactory technique for "bound from free" separation in gastrin radioimmunoassay (RIA). This reagent, however, cannot be used in stoichiometric invertase-peptide conjugate-based enzyme immunoassays. ...

journal_title：Journal of immunological methods

authors： Pillai S,Mohimen A,Kanjilal A

更新日期：1980-01-01 00:00:00

abstract：:Hepatitis B surface antigen (HBsAg)-specific T cells were analysed in 16 healthy individuals vaccinated against hepatitis B, using optimised protocols. HBsAg-specific IFN-gamma producing CD4(+) T cells were found at a similar low frequency (0.01%) within the naive T cell subset, the central and the effector memory T c...

journal_title：Journal of immunological methods

authors： Litjens NH,Huisman M,Baan CC,van Druningen CJ,Betjes MG

更新日期：2008-01-31 00:00:00