Abstract:
:The possibility that immortalisation of in vitro immunised splenocytes may result in hybridomas secreting polyreactive antibodies was investigated. A panel of nine murine hybridomas, secreting IgM(kappa) anti-goat immunoglobulin G (anti-GIgG), was produced by immortalising splenocytes that had been immunised in vitro with GIgG. The ability of the corresponding monoclonal antibodies (Mabs) to bind multiple antigens was investigated using two techniques. First, the affinity constants characterising the interactions of each of the nine Mabs with each of a panel of six antigens were determined. Second, the specific anti-GIgG activities of each hybridoma supernatant and its corresponding affinity-purified IgM fraction were determined and compared. In total, these experiments indicated that eight of the nine hybridomas were polyreactive.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
McMahon MJ,O'Kennedy Rdoi
10.1016/s0022-1759(01)00481-1keywords:
subject
Has Abstractpub_date
2001-12-01 00:00:00pages
27-36issue
1-2eissn
0022-1759issn
1872-7905pii
S0022-1759(01)00481-1journal_volume
258pub_type
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