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A solid-phase enzyme immunoassay for anti-acetylcholine receptor antibody in myasthenia gravis patients.

Abstract：

:A solid-phase enzyme immunoassay for the measurement of anti-acetylcholine receptor antibodies in the sera of patients with myasthenia gravis is reported. Sufficient amounts of acetylcholine receptor for the sensitive detection of anti-acetylcholine receptor antibody were directly fixed to Costar serocluster 96-well EIA plates coated with poly-L-lysine hydrobromide. The solid-phase enzyme immunoassay detected anti-acetylcholine receptor antibodies in 91% of the myasthenia gravis patients including 4 out of 4 ocular type myasthenia patients, anti-acetylcholine receptor antibodies of which were not detectable by the immunoprecipitation assay. Correlation between antibody titers measured by enzyme immunoassay and the immunoprecipitation assay was significant.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Kobayashi N,Sugita H,Terada E,Ghoda A,Okudaira H,Ogita T,Miyamoto T

doi

10.1016/0022-1759(84)90401-0

subject

Has Abstract

pub_date

1984-10-26 00:00:00

pages

267-72

issue

2

eissn

0022-1759

issn

1872-7905

pii

0022-1759(84)90401-0

journal_volume

73

pub_type

杂志文章

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