Hapten-specific naïve B cells are biomarkers of vaccine efficacy against drugs of abuse.

Abstract:

:Vaccination against drugs of abuse shows efficacy in animal models, yet few subjects achieve effective serum antibody titers in clinical studies. A barrier to translation is the lack of pre-vaccination screening assays that predict the most effective conjugate vaccines or subjects amenable to vaccination. To address this obstacle, we developed a fluorescent antigen-based enrichment method paired with flow cytometry to characterize hapten-specific B cells. Using this approach, we studied naïve and activated B cells specific for structurally-related model haptens based on derivatization of the morphinan structure at the C6 position on oxycodone or at the C8 position on hydrocodone, and showing different pre-clinical efficacy against the prescription opioid oxycodone. Prior to vaccination, naïve B cells exhibited relatively higher affinity for the more effective C6-derivatized oxycodone-based hapten (6OXY) and the 6OXY-specific naïve B cell population contained a higher number of B cells with greater affinity for free oxycodone. Higher affinity of naïve B cells for hapten or oxycodone reflected greater efficacy of vaccination in blocking oxycodone distribution to brain in mice. Shortly after immunization, activated hapten-specific B cells were detected prior to oxycodone-specific serum antibodies and provided earlier evidence of vaccine failure or success. Analysis of hapten-specific naïve and activated B cells may aid rational vaccine design and provide screening tools to predict vaccine clinical efficacy against drugs of abuse or other small molecules.

journal_name

J Immunol Methods

authors

Taylor JJ,Laudenbach M,Tucker AM,Jenkins MK,Pravetoni M

doi

10.1016/j.jim.2014.01.010

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

74-86

eissn

0022-1759

issn

1872-7905

pii

S0022-1759(14)00020-9

journal_volume

405

pub_type

杂志文章
  • A rapid immunoadsorbent radioimmunoassay for anti-acetylcholine receptor antibody.

    abstract::Antibody to the human nicotinic acetylcholine receptor has been demonstrated in the disorder myasthenia gravis and is the pathologic factor producing the characteristic symptoms of the disorder. A rapid, quantitative and sensitive radioimmunoassay using human acetylcholine receptor, affinity labeled with iodinated alp...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(81)90089-2

    authors: Tindall RS,Kent M,Wells L

    更新日期:1981-01-01 00:00:00

  • A microcapillary assay (MASS) for macrophage migration inhibition factor.

    abstract::A technically simple microassay for macrophage migration inhibition studies is described. Using simple 0.08 ml of test solution per assay, meaningful statistical analysis via multiple replicates is possible. A novel feature is that test solutions may be stored frozen in the test well until the indicating macrophages a...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(80)90252-5

    authors: Coyle DE,Brammer PW,Halsall HB

    更新日期:1980-01-01 00:00:00

  • Lateral flow-based antibody testing for Chlamydia trachomatis.

    abstract::We describe here a lateral flow-based assay (LFA) for the detection of antibodies against immunodominant antigen Pgp3 from Chlamydia trachomatis, the causative agent of urogenital chlamydia infection and ocular trachoma. Optimal signal detection was achieved when the gold-conjugate and test line contained Pgp3, creati...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2016.05.008

    authors: Gwyn S,Mitchell A,Dean D,Mkocha H,Handali S,Martin DL

    更新日期:2016-08-01 00:00:00

  • Development of a screening system for detection of somatic mutations. II. The use of peptides and insoluble protein fragments in a non-competitive solid-phase enzyme immunoassay.

    abstract::A system proposed for measurement of mutational risk consists in detection of hemoglobin mutations expressed in erythrocytes. For this detection the production of antibodies specific for Hb variants is essential. Recently we reported a sensitive solid-phase EIA for the production and selection of polyclonal and monocl...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(83)90189-8

    authors: Klasen EA,Rigutti A,Bos A,Bernini LF

    更新日期:1983-05-13 00:00:00

  • Analysis of hapten-carrier protein conjugates by nondenaturing gel electrophoresis.

    abstract::Hapten-carrier protein conjugates were made using five different small haptens (MW < 1000), two carrier proteins and two methods of conjugation. Nondenaturing agarose gel electrophoresis was used to demonstrate that when as few as two molecules of a small hapten are attached to the carrier, the conjugate band migrates...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(93)90317-z

    authors: Kamps-Holtzapple C,Carlin RJ,Sheffield C,Kubena L,Stanker L,DeLoach JR

    更新日期:1993-09-15 00:00:00

  • Determination of parameters that characterize effector-target conjugation of human NK and LAK cells by flow cytometry.

    abstract::Effector-target conjugation between different cell populations of human NK cells and K562 tumor cells has been studied from binding isotherms obtained from data of effector (alpha) and target (beta) conjugate frequencies measured by flow cytometry analysis at different effector-to-target ratios. Non-linear and linear ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/s0022-1759(97)00153-1

    authors: Rubio G,Garcia-Garcia J,Galvez J,Lorenzo N,Lajarin F,Garcia-Peñarrubia P

    更新日期:1997-12-01 00:00:00

  • Terasaki-ELISA for murine IgE. III. Determination of concentration and functional affinity by sequential equilibrium binding analysis.

    abstract::A simple Terasaki tray-based ELISA technique with a fluorescent detecting system has been used to determine the affinity of murine IgE antibodies. The system was shown to be sensitive enough to measure affinities in the range of 10(-6)-10(-10) M as well as detect IgE antibodies down to a limit of 0.1 ng/ml. The result...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(89)90031-8

    authors: Pathak SS,Vos Q,Savelkoul HF

    更新日期:1989-09-29 00:00:00

  • Standardization of a micro-cytotoxicity assay for human natural killer cell lytic activity.

    abstract::Cytotoxicity assays are widely used to evaluate the functional activity of NK and T cells against tumour target cells and the release of radioactive sodium chromate from labelled target cells is still the most commonly used marker of target lysis in culture supernatants. We describe here the standardization of a micro...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(94)90104-x

    authors: Mariani E,Monaco MC,Sgobbi S,de Zwart JF,Mariani AR,Facchini A

    更新日期:1994-06-24 00:00:00

  • A micromethod for evaluating the phagocytic activity of human macrophages by ingestion of radio-labelled polystyrene particles.

    abstract::Studies in vitro of human macrophage function in health and disease have been impeded by the difficulty of obtaining such cells in sufficient number. Unlike animal species, the only readily available source of human macrophages are circulating monocytes. Herein, a method is described whereby the phagocytic rate of sma...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(76)90172-1

    authors: Al-Ibrahim MS,Chandra R,Kishore R,Valentine FT,Lawrence HS

    更新日期:1976-03-01 00:00:00

  • Scavenger receptor block as strategy for the identification of bone marrow homing phages by panning in vivo random peptide phage displayed libraries.

    abstract::Surface molecules exclusively expressed by cells of the bone marrow (BM) are candidate targets for delivering therapeutic agents to this tissue. To identify ligands specific for the BM, we performed a series of pannings in vivo with random peptide phage displayed libraries (RPPDL). We could show that phages bind to bo...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/s0022-1759(02)00089-3

    authors: Finger AN,Bisoffi M,Wetterwald A,Gautschi E,Hohenfeld U,Klima I,Stadler BM,Mazzucchelli L,Thalmann GN,Cecchini MG

    更新日期:2002-06-01 00:00:00

  • Real-time biospecific interaction analysis of antibody reactivity to peptides from the envelope glycoprotein, gp160, of HIV-1.

    abstract::A new assay designed to quantitate antibody reactivity to specific peptides using biospecific interaction analysis (BIAcore) has been developed. Peptides of various lengths (15-40 amino acids) and isoelectric points (pI = 4.5-13) were covalently linked (immobilized) to a biosensor and interacted with polyclonal human ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(92)90225-i

    authors: VanCott TC,Loomis LD,Redfield RR,Birx DL

    更新日期:1992-02-05 00:00:00

  • Th1/Th2 biasing effects of vaccination in cattle as determined by real-time PCR.

    abstract::Real-time polymerase chain reaction (PCR) is now becoming an accepted tool for measuring gene expression at the transcriptional level. In this study, a direct comparison between real-time PCR, enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunosorbent spot (ELISPOT) assay was performed. When interferon-...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/s0022-1759(02)00029-7

    authors: Mena A,Ioannou XP,Van Kessel A,Van Drunen Little-Van Den Hurk S,Popowych Y,Babiuk LA,Godson DL

    更新日期:2002-05-01 00:00:00

  • Improved methods for immunohistochemical detection of BrdU in hard tissue.

    abstract::Bromodeoxyuridine (BrdU) is used to label synthesizing DNA and to chase label-retaining cell (LRC). As stem cells divide slowly in adult tissues, they can be visualized as LRCs. In order to identify LRCs in hard tissue, we examined optimal conditions of fixation, demineralization, and DNA denaturation/antigen retrieva...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2008.07.013

    authors: Shimada A,Shibata T,Komatsu K,Nifuji A

    更新日期:2008-11-30 00:00:00

  • Turbidimetric microassay for macrophage-mediated antibody-dependent cellular cytotoxicity.

    abstract::An improved microassay for quantitation of murine macrophage-mediated antibody-dependent cellular cytotoxicity (ADCC) has been developed. The method is based on the turbidimetric measurement of sheep erythrocyte or nucleated (L1210) target cell suspensions at 630 nm with an automatic microtiter plate densitometer. The...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(86)90262-0

    authors: Rummage JA,Chang NS,Leu RW

    更新日期:1986-01-22 00:00:00

  • A simple multiple chamber apparatus for measuring chemotaxis of polymorphonuclear leukocytes utilizing centrifugation of the chambers before incubation.

    abstract::A simple apparatus for measuring chemotaxis is described which contains thirty chambers per unit. Only very few cells and small volumes of chemotactic factor solution are required per chamber. For satisfactory results the apparatus must be centrifuged briefly prior to incubation. Under these conditions results can be ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/s0022-1759(97)90008-9

    authors: Swanson MJ

    更新日期:1977-01-01 00:00:00

  • Chemical synthesis of bispecific monoclonal antibodies: potential advantages in immunoassay systems.

    abstract::To date, the applications of bispecific antibodies in immunoassay and immunocytochemical procedures have been directed at uniting two different biomolecules through the binding of epitopes on each respective substance. In this study, bispecific antibodies were constructed in which both binding sites were directed to t...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(94)90042-6

    authors: Cook AG,Wood PJ

    更新日期:1994-05-16 00:00:00

  • A novel method for measuring CTL and NK cell-mediated cytotoxicity using annexin V and two-color flow cytometry.

    abstract::An assay based on two-color flow cytometry has been developed to measure CTL and NK cell-mediated cytotoxicity. After effector/target cells are incubated together, CTL or NK populations are stained with an effector cell specific PE-conjugated mAb. Subsequently, annexin V-FITC binds to cells expressing phosphatidylseri...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/s0022-1759(98)00038-6

    authors: Goldberg JE,Sherwood SW,Clayberger C

    更新日期:1999-04-22 00:00:00

  • Pure populations of murine macrophages from cultured embryonic stem cells. Application to studies of chemotaxis and apoptotic cell clearance.

    abstract::Embryonic stem cells provide a potentially convenient source of macrophages in the laboratory. Given the propensity of macrophages for plasticity in phenotype and function, standardised culture and differentiation protocols are required to ensure consistency in population output and activity in functional assays. Here...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2012.06.008

    authors: Zhuang L,Pound JD,Willems JJ,Taylor AH,Forrester LM,Gregory CD

    更新日期:2012-11-30 00:00:00

  • Induction of abundant antibody formation with a protein-cellulose complex in mice.

    abstract::A method is described for immunizing mice with a protein-cellulose complex obtained by covalent coupling of antigenic protein molecules to suspended cellulose particles. Primary immunization of the animals with the complex led to a pronounced immune response persisting for 20-30 days. Subsequent administration of the ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(86)90526-0

    authors: Gurvich AE,Korukova A

    更新日期:1986-03-13 00:00:00

  • A capillary diffusion method for determining hemolytic complement activity in undiluted serum .

    abstract::A method is presented for measuring the hemolytic activity of undiluted guinea pig serum. Undiluted serum is placed in contact with a column of sensitized sheep red blood cells in an agar matrix in a capillary tube. After incubation, the length of the zone of lysis is measured. Comparison with a standard curve prepare...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(79)90212-6

    authors: Brockman JA,Bauman N

    更新日期:1979-01-01 00:00:00

  • Repeated fine-needle aspiration of solid tumours in mice allows the identification of multiple infiltrating immune cell types.

    abstract::This paper describes a novel method for following the changes in mouse tumour-infiltrating immune cell populations by repeated sampling of tumours by fine needle aspiration (FNA), followed by flow cytometry. Using this technique we were able to collect samples from P815 mouse mastocytomas, and identify and enumerate s...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2015.06.015

    authors: Carroll CSE,Altin JG,Neeman T,Fahrer AM

    更新日期:2015-10-01 00:00:00

  • Immunohistochemical application of monoclonal antibodies to reveal the structure and localization of carbohydrate antigens. N-acetyllactosamine-related carbohydrate antigens in human biliary epithelial cells.

    abstract::With the use of a panel of monoclonal antibodies against structurally related carbohydrate antigens, finely regulated cellular phenotype of the carbohydrate antigens can be characterized. As a model system, the expression of N-acetyllactosamine-related antigens in human biliary epithelial cells was investigated using ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(88)90364-x

    authors: Okada Y,Tsuji T

    更新日期:1988-09-13 00:00:00

  • Ultra-sensitive immunochromatographic assay for quantitative determination of erythropoietin.

    abstract::An ultra-sensitive quantitative EPO (erythropoietin) lateral flow immunochromatographic test with a detection limit of 1.2 fM (10(-15) M), 0.035 ng EPO/L, which is 50-100 times more sensitive than a corresponding enzyme based immunoassay, is presented. In comparison with commercial lateral flow tests for other analyte...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2008.09.022

    authors: Lönnberg M,Drevin M,Carlsson J

    更新日期:2008-12-31 00:00:00

  • An efficient protocol for in vivo labeling of proliferating epithelial cells.

    abstract::The study of organogenesis, tissue-homeostasis and regeneration requires the precise assessment of in vivo cell proliferation. To this end a host of methods have been developed to detect and quantify DNA synthesis in proliferating cells. These include cell labeling with various nucleotide analogues and fluorescence re...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/j.jim.2018.03.015

    authors: Michel C,Küchler R,Martin I,Kyewski B,Pinto S

    更新日期:2018-06-01 00:00:00

  • A cassette vector for high-level reporter expression driven by a hybrid invariant chain promoter in transgenic mice.

    abstract::A plasmid cassette vector was designed to generate transgenic mice expressing reporter cDNAs at high levels in antigen-presenting cells under the control of the murine invariant chain (Ii) promoter. Analysis of several transgenic mice harboring a chimeric Ii cDNA placed in this vector showed that it can drive expressi...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/s0022-1759(00)00276-3

    authors: van Santen H,Benoist C,Mathis D

    更新日期:2000-11-01 00:00:00

  • Detection of C3bBb-stabilizing activity (C3 nephritic factor) in the serum from patients with membranoproliferative glomerulonephritis.

    abstract::It is known that membranoproliferative glomerulonephritis (MPGN), hypocomplementaemia and C3 nephritic factor (C3NeF) are closely related to each other, and the presence or absence of C3NeF in the serum is important for evaluating the nature of MPGN. However, some difficulties have been encountered in detecting this f...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(90)90234-m

    authors: Ohi H,Watanabe S,Fujita T,Seki M,Hatano M

    更新日期:1990-07-20 00:00:00

  • Determination of a glucose-containing tetrasaccharide in urine by radioimmunoassay.

    abstract::A radioimmunoassay is described that allows rapid determination of a urinary oligosaccharide -- Glc alpha 1-6Glc alpha 1-4Glc alpha 1-4Glc [(Glc)4] -- at concentrations greater than 2 pmol/microliter. Antibodies produced in rabbits immunized with the phenethylamine derivative of (Glc)4 coupled to keyhole limpet hemocy...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(82)90215-0

    authors: Zopf DA,Levinson RE,Lundblad A

    更新日期:1982-01-01 00:00:00

  • Immunoabsorbents prepared from cell membrane antigens: efficiency of incorporation and shedding of protein during use.

    abstract::Immunoabsorbents for the removal of antibodies against cell membrane antigens have been prepared by a variety of techniques in common use. Cells were surface-labelled by lactoperoxidase-catalysed radioiodination, and the different immunoabsorbent preparations were compared in terms of efficiency of incorporation and s...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(78)90222-3

    authors: Zola H

    更新日期:1978-01-01 00:00:00

  • Presence of matrix-specific antibodies in affinity-purified polyclonal antibodies.

    abstract::In general, antigen affinity columns made with commercially prepared activated affinity supports bind antibody specific for the coupled antigen. Nonetheless, in some cases affinity purification may yield antibodies to molecules other than the molecule of interest. In this report, we demonstrate such an occurrence: an ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(91)90246-c

    authors: Sweet SC,Gnegy ME,Welsh MJ

    更新日期:1991-01-24 00:00:00

  • A method for specifically detecting internal immunoglobulin by immunofluorescence.

    abstract::The detection of specifically internal immunoglobulin by staining of fixed preparations of lymphoid cells with appropriate fluorescein-conjugated antisera can be hampered by binding of the conjugates to membrane-bound immunoglobulin. Without preventing staining through membrane-bound immunoglobulin, it is impossible ...

    journal_title:Journal of immunological methods

    pub_type: 杂志文章

    doi:10.1016/0022-1759(79)90209-6

    authors: Mayrhofer G

    更新日期:1979-01-01 00:00:00