abstract：:Highly sensitive and easy detection method for Alzheimer's disease (AD) with a suitable biomarker is mandatory for preventing the factors resulting from AD. This research reports a modified ELISA with graphene for the detection of AD biomarker amyloid beta (Aβ) oligomer. Gold nanoparticle (AuNP) conjugated aptamer was...

journal_title：Journal of immunological methods

authors： Zhao J,Chang W,Liu L,Xing X,Zhang C,Meng H,Gopinath SCB,Lakshmipriya T,Chen Y,Liu Y

更新日期：2021-02-01 00:00:00

abstract：BACKGROUND:The serological screening for celiac disease (CD) is currently based on the detection of anti-transglutaminase (tTG) IgA antibodies, subsequently confirmed by positive endomysial antibodies (EMA). When an anti-tTG IgA positive/EMA IgA negative result occurs, it can be due either to the lower sensitivity of t...

journal_title：Journal of immunological methods

authors： Infantino M,Merone M,Manfredi M,Grossi V,Landini A,Alessio MG,Previtali G,Trevisan MT,Porcelli B,Fabris M,Macchia D,Villalta D,Cinquanta L,D'Antoni F,Iannello G,Soda P,Bizzaro N

更新日期：2021-02-01 00:00:00

abstract：:In addition to their known implication in allergy studies, IgE antibodies are becoming an increasingly interesting antibody class in cancer research. However, large-scale purification of IgE antibodies still poses substantial challenges, as they cannot be purified using techniques commonly used for other immunoglobuli...

journal_title：Journal of immunological methods

authors： Vukovic N,Harraou S,van Duijnhoven SMJ,Zaiss DM,van Elsas A

更新日期：2021-02-01 00:00:00

abstract：INTRODUCTION:Basophils play a major physio-pathological role in hypersensitivity related diseases. Basophils express high affinity Immunoglobulin (Ig) E receptors (FcεRI), IgG and complement regulatory. Basophils also have immunoregulatory activity through interaction with T cells. The aim of this study was to look for...

journal_title：Journal of immunological methods

authors： Berger AE,Durrieu C,Dzviga C,Perrot JL,Lambert C

更新日期：2021-01-22 00:00:00

abstract：:Clostridioides difficile (C. difficile) is the most common cause of nosocomial antibiotic associated diarrhoea. The incidence of C. difficile infection (CDI) has been rising worldwide over the last 20 years with consequent rises in morbidity, mortality and healthcare costs, although the incidence has fallen in the UK ...

journal_title：Journal of immunological methods

authors： Anwar S,Bryan D,Rigsby P,Dougall T,Rijpkema S

更新日期：2021-01-01 00:00:00

abstract：BACKGROUND:ABO subgroups or weaker variants of A or B are group A or B subjects whose erythrocytes give a weak or negative reaction serologically with anti-A or Anti - B antisera respectively. Occurrence of these subgroups may lead to an ABO discrepancy which often puts transfusion services in a quandary. ABO subgroups...

journal_title：Journal of immunological methods

authors： Tiwari AK,Setya D,Arora D,Mehta SP,Aggarwal G,Mitra S

更新日期：2020-12-01 00:00:00

abstract：:A commonly employed method to determine the function of a particular cell population and to assess its contribution to the overall system in vivo is to selectively deplete that population and observe the effects. Using monoclonal antibodies to deliver toxins to target cells can achieve this with a high degree of effic...

journal_title：Journal of immunological methods

authors： Brown K,Meader L,Nowocin A,Edwards LA,Cheung LH,Smith RA,Rosenblum MG,Wong W

更新日期：2020-08-01 00:00:00

abstract：:Intracellular DNA triggers interferon release during the innate immune response. Cyclic GMP-AMP synthase (cGAS) senses intracellular double-stranded DNA not only in response to viral infection but also under autoimmune conditions. Measuring the levels of cyclic GMP-AMP (cGAMP) as a second messenger of cGAS activation ...

journal_title：Journal of immunological methods

authors： Miyakawa S,Okui T,Shiraishi T,Yoshihara T,Hirayama M,Satomi Y,Hamada T,Nishida M,Akimoto C,Sato S

更新日期：2020-05-01 00:00:00

abstract：:Monitoring recency of infection helps to identify current transmission in vulnerable populations for effective disease control. We have established an in-house avidity based hepatitis C virus (HCV) recency assay based on the Monolisa Anti-HCV PLUS Version 3 ELISA kit for use of dried serum/plasma spots (DS/PS) in orde...

journal_title：Journal of immunological methods

authors： Eshetu A,Hauser A,An der Heiden M,Schmidt D,Meixenberger K,Ross S,Obermeier M,Ehret R,Bock CT,Bartmeyer B,Bremer V,Bannert N

更新日期：2020-04-01 00:00:00

abstract：BACKGROUND:Antibodies that target a single tumor antigen fail to cure stage IV cancer patients due to tumor heterogeneity and variable expression of antigen. Tumor cells with insufficient binding of antibody will not undergo antibody induced cytotoxicity. We describe targeting multiple tumor-specific antigens that resu...

journal_title：Journal of immunological methods

authors： Shukla GS,Sun YJ,Pero SC,Krag DN

更新日期：2020-03-01 00:00:00

abstract：:The use of helminth infections as tools to understand the type 2 immune response is a well-established technique and important to many areas of immunological research. The phenotype and function of immune cell populations at the site of infection is a key determinant of pathogen clearance. However, infections with hel...

journal_title：Journal of immunological methods

authors： Webster HC,Andrusaite AT,Shergold AL,Milling SWF,Perona-Wright G

更新日期：2020-02-01 00:00:00

abstract：:Human primary resting CD4+ T cells are difficult to transfect while preserving viability. The present study evaluated gymnotic delivery and RNase H1-dependent gene expression knockdown mediated by antisense oligonucleotides, called GapmeRs. Exposure of primary resting CD4+ T cells to GapmeRs did not cause cell activat...

journal_title：Journal of immunological methods

authors： Abewe H,Deshmukh S,Mukim A,Beliakova-Bethell N

更新日期：2020-01-01 00:00:00

abstract：:IgA antibodies are key immune effectors against invading pathogens but also possess essential immunoregulatory functions. Detecting and quantifying human IgA+ B-cell subsets and secreted IgA molecules is needed for investigating the protective, modulatory and pathophysiologic roles of IgAs. Here, we produced a recombi...

journal_title：Journal of immunological methods

authors： Planchais C,Mouquet H

更新日期：2020-01-01 00:00:00

abstract：:An anti-cocaine monoclonal antibody (mAb) designated h2E2 will soon enter clinical trials for the treatment of cocaine abuse disorders. Importantly, this antibody selectively binds cocaine and its active metabolite, cocaethylene, with high affinity, while binding inactive metabolites with substantially lower affinitie...

journal_title：Journal of immunological methods

authors： Kirley TL,Norman AB,Wetzel HN

更新日期：2020-01-01 00:00:00

abstract：:The clinical value of assessing immunoglobulin (Ig)G and IgA subclasses in addition to the isotypes of soluble Igs in serum has been well established. >20years ago, the International Union of Immunological Societies and the World Health Organization performed collaborative studies in order to validate antibody (Ab) cl...

journal_title：Journal of immunological methods

authors： Blanco E,Perez-Andres M,Sanoja-Flores L,Wentink M,Pelak O,Martín-Ayuso M,Grigore G,Torres-Canizales J,López-Granados E,Kalina T,van der Burg M,Arriba-Méndez S,Santa Cruz S,Puig N,van Dongen JJM,Orfao A

更新日期：2019-12-01 00:00:00

abstract：:Within EuroFlow, we recently developed screening tubes for hematological malignancies and immune deficiencies. Pipetting of antibodies for such 8-color 12-marker tubes however is time-consuming and prone to operational mistakes. We therefore evaluated dried formats of the lymphocytosis screening tube (LST) and of the ...

journal_title：Journal of immunological methods

authors： van der Velden VHJ,Flores-Montero J,Perez-Andres M,Martin-Ayuso M,Crespo O,Blanco E,Kalina T,Philippé J,Bonroy C,de Bie M,Te Marvelde J,Teodosio C,Corral Mateos A,Kanderová V,van der Burg M,Van Hoof D,van Dongen JJM,Orf

更新日期：2019-12-01 00:00:00

abstract：:The EuroFlow Consortium developed a fully standardized flow cytometric approach from instrument settings, through antibody panel, reagents and sample preparation protocols, to data acquisition and analysis. The Swiss Cytometry Society (SCS) promoted a study to evaluate the feasibility of using such standardized measur...

journal_title：Journal of immunological methods

authors： Glier H,Heijnen I,Hauwel M,Dirks J,Quarroz S,Lehmann T,Rovo A,Arn K,Matthes T,Hogan C,Keller P,Dudkiewicz E,Stüssi G,Fernandez P,Swiss Cytometry Society.

更新日期：2019-12-01 00:00:00

abstract：:The rise in the analytical speed of mutiparameter flow cytometers made possible by the introduction of digital instruments, has brought up the possibility to manage progressively higher number of parameters simultaneously on significantly greater numbers of individual cells. This has led to an exponential increase in ...

journal_title：Journal of immunological methods

authors： Pedreira CE,Costa ESD,Lecrevise Q,Grigore G,Fluxa R,Verde J,Hernandez J,van Dongen JJM,Orfao A,EuroFlow.

更新日期：2019-12-01 00:00:00

abstract：:Plasma cells (PCs) secrete antibodies and play an essential role in protective immunity, but also in pathogenesis of antibody-mediated diseases. Physiologically, PCs mainly reside within bone marrow and spleen. In autoimmune diseases such as systemic lupus erythematosus (SLE) autoantibody-producing PCs can also be fou...

journal_title：Journal of immunological methods

authors： Schaffer S,Maul-Pavicic A,Voll RE,Chevalier N

更新日期：2019-11-01 00:00:00

abstract：:Proper evaluation of immunogenicity during clinical development of biotherapeutics is a major challenge to bioanalytical scientists, in part due to matrix interference in anti-drug antibody (ADA) and neutralizing antibody (NAB) assays. If not addressed, matrix interference could confound the immunogenicity assessment ...

journal_title：Journal of immunological methods

authors： Wang Y,Luong M,Guadiz C,Zhang M,Gorovits B

更新日期：2019-11-01 00:00:00

abstract：:We developed a noncompetitive two-site sandwich ELISA to quantitate monoclonal antibodies in culture supernatant. This assay measures the initial enzyme activity rate during the first minute of the reaction, which ensures linear velocity relative to time and a progress curve slope proportional to analyte concentration...

journal_title：Journal of immunological methods

authors： Domínguez M,Moreno I,Toraño A

更新日期：2019-11-01 00:00:00

abstract：:Introduction of a tumor antigen-specific T cell receptor (TCR) into patient-derived lymphocytes has already exhibited promising results for the treatment of melanoma and other malignancies in clinical trials. However, insufficient or unsuccessful ex vivo manufacturing of engineered T cells due to low expansion and/or ...

journal_title：Journal of immunological methods

authors： Simon B,Harrer DC,Thirion C,Schuler-Thurner B,Schuler G,Uslu U

更新日期：2019-09-01 00:00:00

abstract：BACKGROUND:Point of care tests would be valuable for field diagnosis. However, high sensitivity will likely be required in low endemicity sets where individuals with low schistosome burden are hard to diagnose. METHODS:Commercially available POC tests (POC-CCA® and Urine CCA (Schisto) ECO Teste®) were evaluated to evi...

journal_title：Journal of immunological methods

authors： Grenfell RFQ,Pedrosa ML,Couto FFB,Almeida A,Coelho PMZ,Katz N

更新日期：2019-09-01 00:00:00

abstract：:Using the advantages of phenol red, a signal enhancer, and bovine serum albumin (BSA), a stabilizer of horseradish peroxidase (HRP), added in HRP enzyme reaction of Amplex Red and H2O2, highly sensitive 1,1'-oxalyldiimidazole chemiluminescence enzyme immunoassay (ODI-CLEIA) was developed to rapidly quantify trace leve...

journal_title：Journal of immunological methods

authors： Kim SS,Lee Y,Shin HS,Lee JH

更新日期：2019-08-01 00:00:00

abstract：:TNF antagonists such as infliximab are effective for the treatment of several inflammatory and autoimmune diseases. Recent clinical studies have advocated the importance of measuring trough infliximab levels to guide treatment decisions. We have developed a novel assay for measuring serum free infliximab levels using ...

journal_title：Journal of immunological methods

authors： Pavlov IY,Parker RL,Lázár-Molnár E,Strathmann FG,Delgado JC

更新日期：2019-07-01 00:00:00

abstract：OBJECTIVE:The central mechanism for establishing a self-tolerant and functional T cell repertoire includes the promiscuous expression of otherwise tissue-restricted proteins by medullary thymic epithelial cells (TEC). We here demonstrate a novel and highly efficient method for isolating this rare key cell type. METHOD...

journal_title：Journal of immunological methods

authors： Dohr D,Engelmann R,Müller-Hilke B

更新日期：2019-04-01 00:00:00

abstract：:A clonogenic assay system for thymic epithelial cells (TECs) is of crucial importance for identifying thymic epithelial stem and/or progenitor cells, evaluating their activities, and understanding the mechanisms of thymic involution. However, current systems are not sufficiently sensitive at detecting and quantifying ...

journal_title：Journal of immunological methods

authors： Sekai M,Wang J,Minato N,Hamazaki Y

更新日期：2019-04-01 00:00:00

abstract：:IL-21 is a pleiotropic cytokine that plays a key role in modulating inflammatory responses, including the promotion of autoimmune diseases. Several groups have quantitated circulating levels of IL-21 in plasma and serum samples using various commercial ELISAs. We determined, however, that the most commonly used commer...

journal_title：Journal of immunological methods

authors： Poorbaugh J,Samanta T,Bright SW,Sissons SE,Chang CY,Oberoi P,MacDonald AJ,Martin AP,Cox KL,Benschop RJ

更新日期：2019-03-01 00:00:00

abstract：:Sepsis is a systemic inflammatory response ensuing from presence and persistence of microorganisms in the bloodstream. The possibility to identify them at low concentrations may improve the problem of human health and therapeutic outcomes. So, sensitive and rapid diagnostic systems are essential to evaluate bacterial ...

journal_title：Journal of immunological methods

authors： De Plano LM,Fazio E,Rizzo MG,Franco D,Carnazza S,Trusso S,Neri F,Guglielmino SPP

更新日期：2019-02-01 00:00:00

abstract：:Development of cell lines for biotherapeutic protein production requires screening large numbers of clones to identify and isolate high producing ones. As such, stable cell line generation is a time- and resource-intensive process. There is an increasing need to enhance the selection efficiency of high-yielding clonal...

journal_title：Journal of immunological methods

authors： Chakrabarti L,Mathew A,Li L,Han S,Klover J,Albanetti T,Hawley-Nelson P

更新日期：2019-01-01 00:00:00

abstract：:Here we extensively describe a FACS-based protocol for isolating intact non-stained human eosinophils from peripheral blood; a stop forward from our recently published initial study. This method of purification could be accomplished in <3 h with only small volumes of whole blood necessary, even in healthy subjects gen...

journal_title：Journal of immunological methods

authors： Wacht G,Poirot A,Charles AL,Radosavljevic M,Uring-Lambert B,de Blay F,Geny B,Bahram S,Barnig C

更新日期：2018-12-01 00:00:00

abstract：:Antibody-based sensors are now widely used in therapeutics, diagnostics, and in environmental monitoring. Recombinant antibodies are becoming integral parts of such devices due to their reported high affinities, their capacity for engineering to achieve highly defined performance characteristics and the fact that thei...

journal_title：Journal of immunological methods

authors： Murphy C,Stack E,Krivelo S,Breheny M,Ma H,O'Kennedy R

更新日期：2018-12-01 00:00:00

abstract：:Cut point determination is an important aspect of immunogenicity assay development. The cut point can be influenced by a myriad of factors. Key among those is the analytical variability of the assay itself and biological variation due to test samples. Since a smaller cut point value may result in improved sensitivity,...

journal_title：Journal of immunological methods

authors： Kubiak RJ,Zhang J,Ren P,Yang H,Roskos LK

更新日期：2018-12-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) accounts for >700,000 deaths worldwide, largely related to poor rates of diagnosis. Our previous work identified glycoproteins with increased levels of fucosylation in HCC. Plate-based assays to measure this change were compromised by increased levels of heterophilic antibodies with glyc...

journal_title：Journal of immunological methods

authors： Wang M,Shen J,Herrera H,Singal A,Swindell C,Renquan L,Mehta A

更新日期：2018-11-01 00:00:00

abstract：:Ranibizumab (Lucentis®), a humanized antigen-binding fragment (Fab) monoclonal antibody that blocks VEGF-A activity, is currently approved for the treatment of several retinal degenerative diseases. The assessment of drug pharmacokinetics (PK) is essential for evaluating exposure as it relates to drug safety and effic...

journal_title：Journal of immunological methods

authors： Lowe J,Wakshull E,Shek T,Chuntharapai A,Elliott R,Rusit J,Maia M

更新日期：2018-10-01 00:00:00

abstract：:The aim of this study was to compare the results of serological assays using pneumococcal proteins or polysaccharides for the detection of pneumococcal infection in childhood pneumonia. Serological assays measured IgG against eight pneumococcal proteins (Ply,CbpA,PspA1,PspA2,PcpA,PhtD,StkP-C,PcsB-N), C-polysaccharide ...

journal_title：Journal of immunological methods

authors： Borges IC,Andrade DC,Ekström N,Virta C,Melin M,Saukkoriipi A,Leinonen M,Ruuskanen O,Käyhty H,Nascimento-Carvalho CM

更新日期：2018-09-01 00:00:00

abstract：:In the field of sexually transmitted infections (STI), the cervicovaginal explant (CVEx) model, not only provides the opportunity to study the different immunological arms present in these tissues under steady state conditions, but also their response against ex vivo infection with relevant pathogens. The methodology ...

journal_title：Journal of immunological methods

authors： Cantero J,Genescà M

更新日期：2018-09-01 00:00:00

abstract：:A rapid and simple immunostick immunosorbent ELISA has been developed for the diagnosis of myasthenia gravis. It is based on coating immunosticks with the major myasthenia gravis autoantigens acetylcholine receptor (AChR) or MuSK and it can be performed in <1 h, in a non-specialized examination place by non-skilled pe...

journal_title：Journal of immunological methods

authors： Trakas N,Tzartos SJ

更新日期：2018-09-01 00:00:00

abstract：:Cell-based assays to monitor antigen-specific T-cell responses are characterized by their high complexity and should be conducted under controlled conditions to lower multiple possible sources of assay variation. However, the lack of standard reagents makes it difficult to directly compare results generated in one lab...

journal_title：Journal of immunological methods

authors： Bidmon N,Kind S,Welters MJP,Joseph-Pietras D,Laske K,Maurer D,Hadrup SR,Schreibelt G,Rae R,Sahin U,Gouttefangeas C,Britten CM,van der Burg SH

更新日期：2018-07-01 00:00:00

abstract：:The study of organogenesis, tissue-homeostasis and regeneration requires the precise assessment of in vivo cell proliferation. To this end a host of methods have been developed to detect and quantify DNA synthesis in proliferating cells. These include cell labeling with various nucleotide analogues and fluorescence re...

journal_title：Journal of immunological methods

authors： Michel C,Küchler R,Martin I,Kyewski B,Pinto S

更新日期：2018-06-01 00:00:00