Abstract:
:Passage of human peripheral blood leucocytes through Sephadex G-10 columns results in a complete depletion of monocytes. Microscopic examination and ingestion of latex particles failed to reveal any monocytes in the column-passed cells. The study of surface markers shows a slight enrichment of T-cells and a decrease of B-cells that is more pronounced in the IgG- and IgA-bearing lymphocytes. No changes were seen in the Fc receptor-positive cells. Con A-induced activation was significantly affected. Pokeweed mitogen activation was reduced 50% at optimal doses of mitogen whereas the response was unaffected at suboptimal doses. Mixed leucocyte reactions (MLR) were greatly depressed when monocytes were removed from either the responder or the stimulator cells, indicating an important role of monocytes both in the elicitation of the response and as auxiliary cells in the mixed leucocyte culture.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Alonso CM,Bernabé RR,Moreno E,Diaz de Espada Fdoi
10.1016/0022-1759(78)90043-1keywords:
subject
Has Abstractpub_date
1978-01-01 00:00:00pages
361-8issue
3-4eissn
0022-1759issn
1872-7905pii
0022-1759(78)90043-1journal_volume
22pub_type
杂志文章abstract::We sought to develop and optimize a hybridoma-based technology for generating human hybridomas that secrete virus-specific monoclonal antibodies for clinical diagnosis and therapy. We developed a novel electrofusion protocol for efficiently fusing Epstein-Barr virus (EBV)-transformed human B cells with myeloma partner...
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journal_title:Journal of immunological methods
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pub_type: 杂志文章
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更新日期:1985-12-17 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1990-08-28 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1998-06-01 00:00:00
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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更新日期:2007-04-30 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2016.05.001
更新日期:2016-08-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1982-12-17 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(83)90389-7
更新日期:1983-11-11 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(83)90401-5
更新日期:1983-11-11 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(77)90143-0
更新日期:1977-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章,评审
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1988-10-26 00:00:00
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更新日期:2009-10-31 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章,评审
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journal_title:Journal of immunological methods
pub_type: 杂志文章,评审
doi:10.1016/s0022-1759(02)00197-7
更新日期:2002-10-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(91)90163-a
更新日期:1991-04-25 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(79)90313-2
更新日期:1979-01-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(84)90474-5
更新日期:1984-03-16 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:2003-03-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2005.04.007
更新日期:2005-07-01 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(94)00255-u
更新日期:1995-01-27 00:00:00
abstract::Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are pathogens commonly associated with infectious diseases in childhood. This study aimed to develop a fluorescent multiplexed bead-based immunoassay (FMIA) using recombinant proteins for the quantitation of serum IgG antibodies against these ...
journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2014.02.002
更新日期:2014-03-01 00:00:00