Abstract:
:Cytokines and chemokines are soluble mediators of the immune system that play a crucial role in intercellular signaling, and in the recruitment of cells to inflammation sites. Identification of these molecules in nonhuman primates (NHP) is crucial for the understanding of complex physiological and pathological mechanisms that occur in these species, and to demonstrate whether these mechanisms function similarly in humans. The Luminex100 system is a bench-top flow cytometer that allows the user to perform up to 100 tests simultaneously in a single tube. Recently, a significant number of commercial vendors have developed kits for the simultaneous detection of multiple cytokines and chemokines of human origin with the Luminex system. These kits were tested for their capacity to recognize chemokines and cytokines of nonhuman primate origin. ELISA and ELISPOT assays were also adapted to the Luminex format, and novel assays based on new combinations of antibodies were developed. PBMC were isolated from blood from chimpanzees, rhesus macaques, baboons, cynomolgus macaques, pig-tailed macaques, and African green monkeys; these cells were stimulated in vitro and culture supernatants were used for the determination of cytokines and chemokines. Crossreactivity tables were prepared based on the ability of the reagents to detect cytokines and chemokines in NHP samples with similar intensity to the ones observed in human samples. By mixing commercially available reagents and newly developed ones, a combination has been created that allows for the detection of 20 NHP chemokines and cytokines in a single sample, including G-CSF, GM-CSF, IFN-gamma, IL-1beta, IL-1Ra, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 (p40), IL-17, IL-18, MCP-1, MIP-1alpha, MIP-1beta, RANTES, TNF-alpha, and TNF-beta. These reagents may become a very useful resource for scientists working with these NHP species, which are relevant pre-clinical models for human diseases and transplantation because they approximate humans in physiology and genetics more closely than any other animal.
journal_name
J Immunol Methodsjournal_title
Journal of immunological methodsauthors
Giavedoni LDdoi
10.1016/j.jim.2005.03.015keywords:
subject
Has Abstractpub_date
2005-06-01 00:00:00pages
89-101issue
1-2eissn
0022-1759issn
1872-7905pii
S0022-1759(05)00093-1journal_volume
301pub_type
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journal_title:Journal of immunological methods
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journal_title:Journal of immunological methods
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abstract::Stability of single-chain Fvs (scFvs) can be improved by mutagenesis followed by phage display selection where the unstable variants are first inactivated by, for example, denaturing treatment. Here we describe a modified strategy for the selection of stabilized antibody fragments by phage display, based on denaturati...
journal_title:Journal of immunological methods
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更新日期:2005-01-01 00:00:00
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journal_title:Journal of immunological methods
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更新日期:2001-03-01 00:00:00
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journal_title:Journal of immunological methods
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doi:10.1016/0022-1759(94)90264-x
更新日期:1994-03-10 00:00:00
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journal_title:Journal of immunological methods
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pub_type: 杂志文章
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更新日期:2006-10-20 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(94)90279-8
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journal_title:Journal of immunological methods
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doi:10.1016/0022-1759(90)90423-s
更新日期:1990-05-08 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1987-12-04 00:00:00
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pub_type: 杂志文章
doi:10.1016/0022-1759(85)90211-x
更新日期:1985-08-02 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(92)90302-a
更新日期:1992-08-30 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/j.jim.2012.09.008
更新日期:2013-01-31 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(89)90049-5
更新日期:1989-03-10 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of immunological methods
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pub_type: 临床试验,杂志文章,多中心研究
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
pub_type: 杂志文章
doi:10.1016/0022-1759(91)90021-7
更新日期:1991-03-21 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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更新日期:1995-10-26 00:00:00
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journal_title:Journal of immunological methods
pub_type: 杂志文章
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